In vivo最新文献

{"title":"Waikato Environment for Knowledge Analysis (WEKA) as a Data Analysis Method Identifying Potential Hematological Parameters for Early Diagnosis of Cervical Cancer.","authors":"Hung-Ming Chiu, Shih-En Lin, Yen-Wei Chu, Chih-Jung Chen","doi":"10.21873/invivo.13909","DOIUrl":"10.21873/invivo.13909","url":null,"abstract":"<p><strong>Background/aim: </strong>The present study explored the use of Waikato Environment for Knowledge Analysis (WEKA) to analyze hematological parameters for distinguishing potential development and progression of cervical cancer. Specifically, we aimed to identify significant biomarkers capable of differentiating atypical squamous cells of undetermined significance (ASC-US) and low-grade squamous intraepithelial lesions (LSIL) from cervical cancer-negative and advanced conditions such as cervical adenocarcinoma.</p><p><strong>Materials and methods: </strong>Hematological and biochemical data were collected from patients and analyzed using data-mining algorithms available in WEKA. The random forest algorithm was employed to identify patterns among key hematological and biochemical biomarkers, alongside one-way analysis of variance to determine significant alterations in these parameters across cancer-negative, ASC-US, LSIL and adenocarcinoma groups.</p><p><strong>Results: </strong>Random forest was the classifier model that demonstrated superior performance metrics with high recall (1.000) and accuracy (0.843), Matthews correlation coefficient (0.510) and area under the curve (0.708), effectively identifying significant patterns within the datasets. One-way analysis of variance indicated significant alterations in red and white blood cell counts, platelet count, hemoglobin, hematocrit and other white blood cell parameters between cancer-negative, ASC-US, LSIL and adenocarcinoma, emphasizing the role of hematological parameters in identifying progression risk.</p><p><strong>Conclusion: </strong>The consistency in conclusions drawn from data mining and statistical analyses highlight the utility of hematological parameters as potential non-invasive biomarkers for cervical cancer screening and progression monitoring. These findings suggest that integrating machine-learning algorithms, particularly random forest, with hematological analysis might enhance early diagnosis and improve clinical outcomes for patients with cervical abnormalities.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"1042-1053"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of the COVID-19 Pandemic on Ophthalmologists Residents Well-being and Training Programs: A Systematic Review.","authors":"Vasiliki Dimaki, Christos P Zampetidis, Efthimios Vasilopoulos, Dimitrios Papakonstantinou, Klio Chatzistefanou, Marilita M Moschos","doi":"10.21873/invivo.13867","DOIUrl":"10.21873/invivo.13867","url":null,"abstract":"<p><strong>Background/aim: </strong>The COVID-19 pandemic significantly impacted residents' well-being and training programs, with potentially severe consequences for specialties like ophthalmology, where residents were often reassigned to non-specialty-related units and tasks. This study aimed to systematically review the pandemic's effects on ophthalmology residents' well-being and training.</p><p><strong>Patients and methods: </strong>A systematic review of the literature was carried out using MEDLINE, Scopus, and EBSCO databases. After removing duplicates, 173 unique records were identified, and 30 were considered as potentially relevant. Of these, 13 studies met the inclusion and exclusion criteria and were further analyzed.</p><p><strong>Results: </strong>The reviewed studies revealed a notable decline in both theoretical and practical aspects of residency training during the pandemic. These impacts were supported by both objective data (<i>e.g.</i>, the number of surgeries carried out during the training), and residents' self-reported experiences. The pandemic also had a negative impact on residents' mental health, as evidenced by comparative analyses of data collected prior and during the pandemic. The studies highlighted a correlation between the severity of training disruptions and the negative effects on mental health.</p><p><strong>Conclusion: </strong>Education programs should be implemented to cover the knowledge gap of those who were residents during the pandemic. Additionally, targeted mental health support initiatives are recommended to mitigate the long-term impacts on residents' psychological well-being.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"621-633"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Preoperative Immune Prognostic Index for Predicting Outcomes in Patients Undergoing Radical Nephroureterectomy for Upper Tract Urothelial Carcinoma.","authors":"Ken Shibata, Kosuke Iwatani, Y U Imai, Kentaro Yoshihara, Keiichiro Miyajima, Wataru Fukuokaya, Kagenori Ito, Taro Igarashi, Takafumi Yanagisawa, Kojiro Tashiro, Shusuke Tsuzuki, Steffi Kar Kei Yuen, Jeremy Yuen-Chun Teoh, Brendan A Yanada, Yusuke Koike, Tatsuya Shimomura, Hiroki Yamada, Akira Furuta, Jun Miki, Takahiro Kimura, Fumihiko Urabe","doi":"10.21873/invivo.13885","DOIUrl":"10.21873/invivo.13885","url":null,"abstract":"<p><strong>Background/aim: </strong>The lung immune prognostic index (LIPI), which is determined by assessing the derived neutrophil-to-lymphocyte ratio in conjunction with the level of lactate dehydrogenase, predicts outcomes in various cancers. Its utility as a preoperative biomarker in upper tract urothelial carcinoma (UTUC) patients remains unexplored.</p><p><strong>Patients and methods: </strong>This is a retrospective study of UTUC patients who underwent radical nephroureterectomy. Patients were stratified into favorable, intermediate, and poor LIPI groups. Non-urothelial tract recurrence-free survival (NUTRFS) was evaluated using Kaplan-Meier analysis, and Cox regression analyses were performed to identify risk factors for NUTRFS.</p><p><strong>Results: </strong>A total of 567 UTUC patients were included, with 46.4% in the favorable, 44.6% in the intermediate, and 9.0% in the poor (9.0%) LIPI groups. The median participant age was 74 years, with a median follow-up of 26 months. Kaplan-Meier curves demonstrated that NUTRFS was significantly worse in patients with poor and intermediate LIPI scores than in those with favorable LIPI scores. Furthermore, combining LIPI status with pathological stages (pT3-4/ypT2-4) and lymph node metastasis status improved postoperative non-urothelial tract recurrence prognosis assessment.</p><p><strong>Conclusion: </strong>The preoperative LIPI is a prognostic indicator for patients with UTUC undergoing nephroureterectomy. Its predictive accuracy improves when combined with advanced pathological stages and lymph node metastasis status.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"824-833"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential Common Molecular Mechanisms Between Periodontitis and Prostate Cancer: A Network Analysis of Differentially Expressed miRNAs.","authors":"Aylin Kanli, Deniz Sunnetci-Akkoyunlu, Nurhan Kulcu-Sarikaya, Cansu Ugurtaş, Gurler Akpinar, Murat Kasap","doi":"10.21873/invivo.13863","DOIUrl":"10.21873/invivo.13863","url":null,"abstract":"<p><strong>Background/aim: </strong>Prostate cancer is the second leading cause of cancer-related deaths in men. Periodontitis is considered a high-risk factor for prostate cancer, but the genetic mechanism is unclear. This study aims to identify dysregulated miRNAs, their associated genes, signaling pathways, and compounds linking periodontitis to prostate cancer.</p><p><strong>Materials and methods: </strong>The miRNA expression datasets of prostate cancer and periodontitis were obtained from the GEO database. Differentially expressed miRNAs (DEmiRNAs) were identified, and common DEmiRNAs (Co-DEmiRNAs) between both datasets were determined. The Co-DEmiRNA-target network structure and functional analyses, including miRNet 2.0, were performed, encompassing Co-DEmiRNA-gene, Co-DEmiRNA-transcription factor (TF), and Co-DEmiRNA-compound networks. Functional enrichment analysis for Co-DEmiRNA genes and Co-DEmiRNA-TF networks was conducted using KEGG, Reactome pathways, and Gene Ontology (GO). Co-up and co-down DEmiRNAs were validated with TCGA miRNA-seq data.</p><p><strong>Results: </strong>hsa-mir-148a-3p, hsa-mir-148b-5p, and hsa-mir-623 are the top miRNA nodes in Co-DEmiRNA-Target networks. The most significant candidate miRNA dysregulation genes are POU2F1, TMOD3, SCD, PRRC2C, and MAT2A, while the most important dysregulation TF includes TP53, CREB1, DNMT1, E2F1, and EGR1. Arsenic trioxide, gemcitabine, and 1,2,6-tri-O-galloyl-beta-D-glucopyranose are the most correlated compounds. Functional analyses revealed multiple cell signaling pathways, such as NOTCH and CREB phosphorylation, and regulation of processes, such as RNA metabolism and transcription.</p><p><strong>Conclusion: </strong>Our study suggests candidate molecular mechanisms linking periodontitis to prostate cancer, highlighting potential compounds targeting both diseases. These findings provide a foundation for guiding future basic and clinical research.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"795-809"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Claudin 18.2 Is Not a Promising Biomarker for Targeted Immunotherapy in Prostatic Cancers.","authors":"Jina Baek, Young Ran Shim, Mi-Jin Gu","doi":"10.21873/invivo.13884","DOIUrl":"10.21873/invivo.13884","url":null,"abstract":"<p><strong>Background/aim: </strong>Claudin 18.2 (CLDN18.2) has emerged as a highly selective biomarker and its abnormal expression has been reported in various primary malignant tumors. Recently, CLDN18.2 has gained attention as both a prognostic biomarker and a potential therapeutic target in gastric and gastroesophageal junction cancer. The purpose of this study was to investigate CLDN18.2 expression in a large cohort of 168 prostate cancer (PC) tissues, assess its correlation with clinicopathological factors, and evaluate its potential as a therapeutic target for anti-CLDN18.2 therapy.</p><p><strong>Materials and methods: </strong>Immunohistochemical staining was conducted using an approved diagnostic CLDN18.2 immunohistochemistry protocol (Ventana, 43-14A clone). Interpretation was carried out following the general rules for staining evaluation.</p><p><strong>Results: </strong>Non-neoplastic gastric mucosa, used as a positive control for CLDN18.2 immunostaining, exhibited strong membranous staining. In contrast, no CLDN18.2 expression was detected in normal ductal and acinar cells of prostate tissue, nor in any of the 168 variable PC tissues.</p><p><strong>Conclusion: </strong>CLDN18.2 is not expressed in normal prostate tissue or PC, suggesting that it is unlikely to serve as a prognostic marker or a potential target for immunotherapy in PC.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"819-823"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Delayed Cortisol Peak Effect After Severe Burn Injury: A Prospective Observational Study.","authors":"Maximilian Bamberg, Máté Elod Maros, Maximilian Michael Menger, Johannes Tobias Thiel, Claudius Illg, Tim Viergutz, Armin Kalenka, Johann Fontana","doi":"10.21873/invivo.13891","DOIUrl":"10.21873/invivo.13891","url":null,"abstract":"<p><strong>Background/aim: </strong>In contrast to patients with major trauma and septic shock, little is known about the incidence and relevance of acute adrenal insufficiency among patients with burn injuries. Addressing this data gap, the current study was designed to analyze the spontaneous cortisol stress response after severe burn injury.</p><p><strong>Patients and methods: </strong>The study was designed as a prospective observational analysis. From 09/2023 until 06/2024, all patients admitted to the Intensive Care Unit of the BG Tübingen Burn Center were included. The daily cortisol levels were determined in combination with the daily routine laboratory values between 5 and 6 a.m. from day 1 until 7. The data acquisition included the following parameters: Age, sex, total body surface area (TBSA) burned, Abbreviated Burn Severity Index (ABSI), tracheotomy, inhalation trauma, serum cortisol, interleukin-6 (IL-6), leukocyte count and C-reactive protein (CRP).</p><p><strong>Results: </strong>A total of 19 patients with a mean TBSA burned of 33.58±22.68% were included. The mean cortisol values from days 1 to 4 stayed within the physiological range with a delayed significant increase from day 5 until 7 (day 1: 503.3±279.5 <i>vs.</i> day 7: 812.5±330.6 nmol/l; physiological range=130-630 nmol/l). TBSA burned, ABSI, and third-degree injury failed to demonstrate predictive potential, while the IL-6 and leukocyte courses demonstrated significant correlation with the cortisol course.</p><p><strong>Conclusion: </strong>The findings of the current study align with previous studies suggesting a relative cortisol deficit during the acute stages of severe burn injury, supporting the treatment approach of early low-dose hydrocortisone supplementation in patients with severe burn injury.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"877-884"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility of a \"Stomach-preserving Strategy\" for Perforated Gastric Cancer in Patients With Distant Metastasis.","authors":"Masayoshi Terayama, Koshi Kumagai, Motonari Ri, Rie Makuuchi, Masaru Hayami, Satoshi Ida, Manabu Ohashi, Takeshi Sano, Souya Nunobe","doi":"10.21873/invivo.13902","DOIUrl":"10.21873/invivo.13902","url":null,"abstract":"<p><strong>Background/aim: </strong>Gastrectomy is often performed for perforated gastric cancer in patients receiving treatment with curative intent. However, gastrectomy is not a curative procedure, precludes oral intake, and may hinder palliative chemotherapy in patients with metastatic disease. The present study assessed the feasibility of a \"stomach-preserving strategy\" comprising peritoneal lavage and repair surgery for the management of gastric cancer perforation in patients with distant metastasis.</p><p><strong>Patients and methods: </strong>We retrospectively reviewed the medical records of patients with gastric cancer who underwent surgical treatment at our hospital from 2013 to 2021. The clinical courses of patients who had undergone peritoneal lavage and repair surgery for perforated gastric cancer with distant metastasis were reviewed to evaluate postoperative outcomes.</p><p><strong>Results: </strong>During the study period, 3,862 patients underwent radical gastrectomy. Additionally, nine patients with stage IV gastric cancer with distant metastasis prior to treatment underwent emergency surgery due to gastric perforation. Of the nine patients that underwent emergency surgery, seven patients underwent peritoneal lavage and repair surgery and two underwent peritoneal lavage only. No cases of secondary leakage were observed. Seven patients (78%) had a good postoperative course including the resumption of meals and continuation of chemotherapy. The remaining two died of sepsis. The median overall survival time was five months from surgery and 12 months from the initiation of palliative chemotherapy.</p><p><strong>Conclusion: </strong>A \"stomach-preserving strategy\" for the management of perforated gastric cancer is safe in patients with stage IV gastric cancer with distant metastasis and allows continuation of oral intake and palliative chemotherapy.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"969-975"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flow Cytometry for Assessing Human Sperm Acrosomal Reaction (AR) and Correlation of AR and Acrosomal Enzyme Activity With Semen Parameters.","authors":"Zubin He, Jinliang Duan, Xin Zhou, Qingdi Quentin Li, Zhuguang Liang, Honglin Chen, Jian Xie, Jiaqiang Luo, Yaobin Wang, Guangzhi Liu, Meifang Zeng","doi":"10.21873/invivo.13896","DOIUrl":"10.21873/invivo.13896","url":null,"abstract":"<p><strong>Background/aim: </strong>This study aimed to evaluate human sperm acrosomal reaction (AR) using flow cytometry and examine the correlation of AR and acrosomal enzyme activity (AEA) with seven semen parameters: total sperm count, concentration, vitality, motility, morphology, survival rate, and sperm DNA fragmentation index (DFI).</p><p><strong>Patients and methods: </strong>A retrospective analysis was conducted on semen data from 398 men in the AR group and 526 men in the AEA group. AR was assessed using flow cytometry, while AEA was measured with the solid-phase BAPNA method. Correlations between AR, AEA, and the seven semen parameters were analyzed. Subgroups based on standard reference values were also compared.</p><p><strong>Results: </strong>AEA demonstrated significant positive correlations with total sperm count, concentration, vitality, motility, morphology, and survival rate, and a negative correlation with DFI (<i>p</i><0.05). In contrast, AR correlated only with sperm concentration. Subgroup analyses revealed that in the AR group, the lower subgroup correlated with concentration and vitality, while the normal subgroup correlated only with concentration. In the AEA group, the normal subgroup showed significant correlations with all seven parameters, while none were found in the lower subgroup. The normal subgroup exhibited significantly higher total count, concentration, vitality, motility, morphology, and survival rate, and lower DFI (<i>p</i><0.05). No significant differences were observed between the normal and lower AR subgroups, except for survival rate (<i>p</i>>0.05).</p><p><strong>Conclusion: </strong>AEA shows stronger and more consistent correlations with semen parameters compared with AR, suggesting its potential as a reliable marker for evaluating male fertility. Further research is needed to clarify the predictive value of AR in male fertility assessment.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"917-926"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Patient-specific Quality Assurance in Carbon-ion Radiation Therapy: Recalculating Delivered Dose Distribution Using Log Data.","authors":"Yongdo Yun, Min Cheol Han, Changhwan Kim, Chae-Seon Hong, Dong Wook Kim, H O Lee, Hojin Kim, Soorim Han, Hikaru Souda, Sung Hyun Lee, Yuya Miyasaka, Takeo Iwai, Jin Sung Kim","doi":"10.21873/invivo.13913","DOIUrl":"10.21873/invivo.13913","url":null,"abstract":"<p><strong>Background/aim: </strong>Log data from radiation treatment machines can play a crucial role in quality assurance by enabling the recalculation of the delivered dose distribution and identification of deviations in treatment delivery. This article proposes a novel method for recalculating the delivered dose distribution in carbon-ion radiation therapy using log data.</p><p><strong>Materials and methods: </strong>The proposed approach leverages existing functionality in commercial treatment planning systems, thus eliminating the need for specialized in-house software for dose calculation and evaluation. The performed tests entail data generation from actual log files using the Digital Imaging and Communications in Medicine standard, biological dose calculations, and gamma evaluations.</p><p><strong>Results: </strong>The log-based approach demonstrated notable advantages, including improved time efficiency and the ability to calculate three-dimensional biological doses. By recalculating the delivered dose distributions, our method improves quality assurance accuracy in carbon-ion radiation therapy. It complements conventional measurement-based patient-specific quality assurance methods, serving as a valuable addition to the arsenal of tools available for treatment evaluation.</p><p><strong>Conclusion: </strong>We believe that our method has the potential to enhance the efficacy of patient-specific quality assurance and contribute significantly to the advancement of carbon-ion radiation therapy, thereby reinforcing the ongoing evolution of particle therapy.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"1086-1093"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Unexpected Relationship Between Human Papillomavirus and Head and Neck Squamous Cell Carcinoma: A Mendelian Randomization Study.","authors":"Yong Feng, Juan Zhai, Tobias Vollkommer, Martin Gosau, Ralf Smeets, Rico Rutkowski, Jieheng Wu, Liyuan Xiao, Reinhard E Friedrich","doi":"10.21873/invivo.13918","DOIUrl":"10.21873/invivo.13918","url":null,"abstract":"<p><strong>Background/aim: </strong>Head and neck cancer (HNC) is the sixth most common type of cancer worldwide. While it is widely accepted that human papillomavirus (HPV) infection is a crucial risk factor for the development of this type of cancer, there is still a lack of support from large-scale studies. This article addresses this gap by comprehensively analyzing the causal relationship between HPV infection and head and neck cancer using Mendelian randomization methods.</p><p><strong>Patients and methods: </strong>The HPV database was utilized to identify instrumental variables <i>via</i> the most enormous GWAS database search tool. The study examined five cancer groups of data of interest from the UK Biobank and three groups of cancer data from the GAME-ON network, all obtained from public databases. The causal relationship was estimated using various approaches, including IVW, weighted median, MR-Egger, simple mode, and weighted mode.</p><p><strong>Results: </strong>The results indicated no causal relationship between HPV16/18 E7 proteins and head and neck cancer, oral cancer, oropharyngeal cancer, and laryngeal cancer, including UKB database and GEME-ON network. Furthermore, there was no heterogeneity or horizontal pleiotropy in the data.</p><p><strong>Conclusion: </strong>The analysis does not support a causal relationship between HPV infection and HNC, as indicated by the lack of evidence linking HPV16/18 E7 proteins to the development of this cancer.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"1134-1147"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}