Transcriptome Analysis of the Effects of X-Ray Radiotherapy on Non-small-cell Lung Cancer Using Next-generation Sequencing.

IF 1.8 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

In vivo Pub Date : 2025-09-01 DOI:10.21873/invivo.14070

Serhat Aras, Tuğba Kul Köprülü, Burçin Erkal Çam, Jülide Balkan, Esra Erdem, Esra Çikler, Altan Kara

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Abstract

Background/aim: This study investigated the acute effects of flattening filter (FF) and flattening filter-free (FFF) beams on gene expression in non-small-cell lung cancer (NSCLC).

Materials and methods: Thirty-six adult athymic nude mice were divided into five groups. The control group did not undergo any radiotherapy or treatment procedures, whereas in the lung cancer (LCa) group, a cancer model was created but not irradiated. LCa models received 20 Gy radiotherapy with FF at 400 MU/min, or with FFF at 1,000 or 1,800 MU/min dose rates. The mice were irradiated 20 days after A549 cancer cell-line implantation and sacrificed 48 h after irradiation for genetic analysis.

Results: Twelve genes were identified as being common across all radiotherapy groups. The expression of most of these genes changed as the dose rate increased. Seven of these genes were also common to the LCa and control groups. Three genes down-regulated in the untreated cancer group showed increased expression with higher dose rates in treated groups. Significant differences were observed in glutamatergic synapse, actin cytoskeleton regulation, and steroid synthesis in FF-400 and FFF-1000. The FFF-1800 group exhibited significant changes in RNA transport, actin cytoskeleton regulation, and phagosome-associated pathways.

Conclusion: FFF beams induced more extensive and pronounced gene-expression changes compared to FF beams in NSCLC.

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利用下一代测序技术分析x射线放疗对非小细胞肺癌的影响。

背景/目的：本研究探讨了扁平滤光剂（FF）和无扁平滤光剂（FFF）对非小细胞肺癌（NSCLC）基因表达的急性影响。材料与方法：将36只成年胸腺裸鼠分为5组。对照组不进行任何放疗或治疗程序，而肺癌（LCa）组则建立了癌症模型，但未进行放疗。LCa模型接受20 Gy放疗，FF剂量率为400 MU/min， FFF剂量率为1000或1800 MU/min。A549癌细胞植入后20天照射小鼠，照射后48 h处死进行遗传分析。结果：12个基因被鉴定为所有放疗组的共同基因。这些基因的表达随着剂量率的增加而改变。其中7个基因在LCa组和对照组中也很常见。在未治疗的癌症组中下调的三个基因在治疗组中随着剂量率的增加而表达增加。FF-400和FFF-1000在谷氨酸突触、肌动蛋白细胞骨架调节和类固醇合成方面存在显著差异。FFF-1800组在RNA转运、肌动蛋白细胞骨架调节和吞噬体相关途径上表现出显著变化。结论：FFF束在NSCLC中诱导的基因表达变化比FF束更广泛、更明显。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

In vivo 医学-医学：研究与实验

CiteScore

4.20

自引率

4.30%

发文量

330

审稿时长

3-8 weeks

期刊介绍： IN VIVO is an international peer-reviewed journal designed to bring together original high quality works and reviews on experimental and clinical biomedical research within the frames of physiology, pathology and disease management. The topics of IN VIVO include: 1. Experimental development and application of new diagnostic and therapeutic procedures; 2. Pharmacological and toxicological evaluation of new drugs, drug combinations and drug delivery systems; 3. Clinical trials; 4. Development and characterization of models of biomedical research; 5. Cancer diagnosis and treatment; 6. Immunotherapy and vaccines; 7. Radiotherapy, Imaging; 8. Tissue engineering, Regenerative medicine; 9. Carcinogenesis.