In Vitro Cellular & Developmental Biology - Plant最新文献

{"title":"Induction of somatic embryogenesis and ectopic proliferation in Tecoma stans (L.) Juss. ex Kunth cell suspension culture","authors":"Rowida Omar, Ivan Kushkevych, Mohamed Abd El-Salam","doi":"10.1007/s11627-024-10421-4","DOIUrl":"https://doi.org/10.1007/s11627-024-10421-4","url":null,"abstract":"<p>Somatic embryogenesis is a developmental pathway where somatic cells of plants generate embryogenic cells that subsequently mature into somatic embryos under favorable conditions. This process is one of the most important <i>in vitro</i> techniques for plant propagation, with diverse practical implications. In this study, ectopic proliferation and somatic embryos from <i>Tecoma stans</i> (L.) Juss. ex Kunth cell cultures were induced by employing primary conditioning Murashige and Skoog medium supplemented with 1.0 mg L⁻¹ 2,4-dichlorophenoxyacetic acid. Subsequently, a secondary induction medium supplemented with a combination of 1.0 mg L⁻¹ 2,4-dichlorophenoxyacetic acid with various concentrations of 6-benzyladenine cytokinin (1 to 5 mg L⁻¹) was used to promote embryogenesis. The results revealed the successful formation of pre-embryonic and embryonic stages, including globular, heart, torpedo, and cotyledon stages within a 2-wk incubation period under the specified hormonal conditions, leading to subsequent development into the mature vegetative phase after an additional 4 wk. Significant embryo production (16 ± 2.0 torpedo stage embryos per 50 mL culture media) was observed in Murashige and Skoog medium enriched with 1.0 mg L⁻¹ 2,4-dichlorophenoxyacetic acid and 2.0 mg L⁻¹ 6-benzyladenine, surpassing the results observed with other concentrations (<i>p</i>-value &lt; 0.0001). The generated somatic embryos can serve as a potential <i>in vitro</i> tool for the propagation, generation, and organogenesis of <i>T. stans</i>, contributing to its role as both an ornamental and medicinal plant. Moreover, the induction of somatic embryogenesis opens avenues for the potential production of <i>T. stans</i> bioactive secondary metabolites and diverse applications in biotechnology, biotransformation, and biocatalysis, particularly in the conversion of both exogenous and endogenous substrates, such as tecomine—the principal antidiabetic alkaloid in the leaf extract.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"59 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140616217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alternative system for micropropagation of Prunus campanulata (Maxim.)","authors":"Daniela Sanson, Alexandre Techy de Almeida Garrett, Isy Cavalhães Rodrigues, Gabriel de Magalhães Miranda, Fabricio William de Ávila, Cristiane Alves Fogaça, Ezequiel Gasparin, Rogério Bobrowski, Fabiana Schmidt Bandeira Peres","doi":"10.1007/s11627-024-10424-1","DOIUrl":"https://doi.org/10.1007/s11627-024-10424-1","url":null,"abstract":"<p>Herein, an <i>in vitro</i> multiplication protocol for <i>Prunus campanulata</i> was evaluated. The effects of 15.0, 30.0, and 45.0 g L⁻¹ sucrose and three flask sealing methods (PVC film, rigid polypropylene lid, and rigid lid with membrane) were analyzed at the multiplication, rooting, and acclimatization stages. For <i>in vitro</i> multiplication, shoot tips of approximately 1 cm in length containing two pairs of leaves from seedlings germinated <i>in vitro</i> in Woody Plant Medium (WPM) were used. Evaluations considered the multiplication of shoot apexes in three subcultures (at 40, 80, and 120 d) for survival, number of shoots, shoot length, number of leaves, and multiplication rate. In the rooting phase, the rooting percentage, number of roots, and root length were assessed. In the acclimatization phase under shaded and full sun conditions, seedling survival and biometric characteristics were evaluated, including stem diameter, height, and number of leaves. The addition of sucrose to the medium, at a concentration of 45.0 g L⁻¹, associated with sealing using PVC plastic film, showed inadequate results for <i>in vitro</i> multiplication of <i>P. campanulata</i>. The addition of sucrose to the culture medium in concentrations ranging from 15.0 to 30.0 g L⁻¹ favored <i>in vitro</i> rooting. The use of a permeable membrane for sealing enabled the growth of more vigorous shoots in terms of percentage of rooting and root length, and these seedlings stood out in the acclimatization phases with greater rates of survival and improved biometric characteristics.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"55 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140595474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Soybean androgenesis II: non-gametophytic morphologies in isolated microspore culture","authors":"Brett Hale, Callie Phipps, Naina Rao, Caroline Kelley, Gregory C. Phillips","doi":"10.1007/s11627-024-10422-3","DOIUrl":"https://doi.org/10.1007/s11627-024-10422-3","url":null,"abstract":"","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"8 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140595532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth of in vitro–regenerated plants of Gerbera jamesonii following micropropagation in temporary immersion bioreactors","authors":"","doi":"10.1007/s11627-024-10429-w","DOIUrl":"https://doi.org/10.1007/s11627-024-10429-w","url":null,"abstract":"<h3>Abstract</h3> <p>Gerbera represents one of the top five most important traditional cut flowers. A major factor influencing the success of the cut flower industry is vase life which in turn has a significant influence on consumer preference. Therefore, the present study considered the effect of silver nanoparticles (AgNPs) supplied in temporary immersion bioreactors (TIBs) on the growth and subsequent shelf life of gerbera flowers. The results showed an initial lag in the growth of plantlets produced from tissue culture, but this effect was reversed by the end of the study, particularly for plants exposed to AgNPs. Although AgNPs did not shorten the time to flowering (67 d in control plants compared with 73 d in AgNPs-treated plants), flower characteristics were not adversely affected by this delay. A significant finding from this study was the observation of improved vase life of flowers grown from plants treated with AgNPs (9.8 d) relative to control plants (8.2 d). This indicated the potential of AgNPs to sustain the longevity of cut flowers after harvest.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"207 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140595364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardization of in vitro multiplication technique for Areca concinna Thwaites, an endangered palm species for its conservation and utilization","authors":"Aparna Veluru, Neema Mohamed, Sandip Shil, Krishna Prakash, K. Kavya, S. Anand, Sudha Raju","doi":"10.1007/s11627-024-10425-0","DOIUrl":"https://doi.org/10.1007/s11627-024-10425-0","url":null,"abstract":"<p><i>Areca concinna</i> Thwaites (Arecaceae) is an endangered plant species endemic to South-Western Sri Lanka and distributed primarily in Sri Lanka, India, and Southeast Asia. This palm was listed under endangered plants species by IUCN (The International Union for Conservation of Nature) due to habitat loss. Clonal propagation is essential for conservation and maintenance of this endangered species. In an effort to conserve this plant species, a protocol was developed for clonal multiplication of <i>A. concinna</i> Thwaites. Immature inflorescence, immature embryos, and mature embryos were tested for callogenesis and subsequent somatic embryogenesis in M72 basal medium supplemented with the auxins 2,4-D and picloram. Of the three explants, somatic embryos were obtained only from mature embryos. Callus multiplication and somatic embryo formation in M72 basal medium supplemented with 2,4-D were found to be better over picloram media. Serial transfer of explants from higher to lower concentrations was essential for sustaining the multiplication of callus as well as for induction of somatic embryos. Somatic embryos grown in Y3 or 1/2 Y3 basal medium supplemented with 1.0 mg L⁻¹ BAP, 0.5 mg L⁻¹ NAA, and 0.25 mg L⁻¹ IBA exhibited shoot initiation and plantlet development. This protocol has its application in rapid multiplication of <i>A. concinna</i> Thwaites palms thereby enhancing the possibility of conservation of the endangered palm.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"69 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140313362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-temperature storage in dark condition improved the in vitro regeneration of Plumbago zeylanica synthetic seeds: a medicinally valuable species","authors":"Ravishankar Chauhan, Priya Banjare, Subir Kumar Parey, Afreen Anjum, Afaque Quraishi","doi":"10.1007/s11627-024-10416-1","DOIUrl":"https://doi.org/10.1007/s11627-024-10416-1","url":null,"abstract":"<p>Medicinal applications of <i>Plumbago</i> <i>zeylanica</i> and its metabolites on various diseases and low viability and inconsistent germination of its seeds are the reasons behind the loss of its genetic diversity. Hence, an efficient protocol for the short-term storage of <i>P.</i> <i>zeylanica</i> synthetic seeds, which is an overexploited medicinally valuable plant, was developed. Initially, <i>in vitro</i> culture was performed from nodal explants to develop synthetic seeds from its proliferated shoots. Murashige and Skoog (MS) medium augmented with 0.5 mg L⁻¹ 6-benzylaminopurine (BAP) resulted in the best morphogenetic response. Thereafter, the developed synseeds were stored for 2 wk at a temperature of 10 or 25°C in different conditions and further evaluated for regeneration. Higher re-growth rate (80%) and the identical morphogenetic response were recorded for the <i>P.</i> <i>zeylanica</i> synthetic seeds, which were stored at a temperature of 10°C in dark condition after its storage period. As per the available literature, this is the first report pertaining to <i>in vitro</i> low-temperature storage of synthetic seeds of <i>P.</i> <i>zeylanica</i> and can further be utilized for the conservation of elite clones for the study of medicinally potent species.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"8 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140100003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different healing agents on acclimatization success of in vitro rooted Garnem (Prunus dulcis × Prunus persica) rootstock","authors":"Heydem Ekinci, Necla Saskin, Bekir Erol Ak, Birgul Dikmetas Dogan","doi":"10.1007/s11627-024-10420-5","DOIUrl":"https://doi.org/10.1007/s11627-024-10420-5","url":null,"abstract":"<p>Continuing productivity of the acclimatization stage of plantlets means successful micropropagation. Due to the free water and high humidity in the culture container environment, poorly developed cuticle results in rapid water loss and drying of plantlets with watery stems and leaves, incomplete stomata, and large intercellular space. When plantlets are exposed to an environmental condition different from the culture medium, problems, such as rapid water loss and drying, may be encountered, and the survival rate of plantlets <i>in vitro</i> may be reduced. The aim of this study was to reduce the stress factors that occur during the acclimatization phase. For this reason, various healing agents have been used. Some of these compounds are ascorbic acid (AsA), salicylic acid (SA), and sodium nitroprusside (SNP). In the study, the response of AsA (100.0 and 200.0 mg L⁻¹), SA (100.0 and 200.0 mg L⁻¹), and SNP (100.0 and 200.0 µM) applications on growth parameters (survival rate (%), node count (pieces per plant), count of leaves (pieces per plant), shoot length (mm), and shoot diameter (mm)) and physiological variable (Soil Plant Analysis Development (SPAD)) were examined. The AsA100 (100.0 mg L⁻¹ ascorbic acid), AsA200 (200.0 mg L⁻¹ ascorbic acid), and SNP100 (100.0 µM sodium nitroprusside) applications resulted in an improvement in node count, leaf count per plant, shoot length, and shoot diameter parameters. The highest shoot length (60.50 ± 7.81 mm) and node count (16.83 ± 1.15 pieces per plantlet) were achieved with the AsA100 application. The maximum stem diameter (2.31 ± 0.37 mm) was determined with the SNP100 application. There were no statistically significant differences found in the survival rate, leaf count, and SPAD parameter. The current study determined that AsA, SA, and SNP applications were effective in regulating several growth parameters in Garnem plantlets and in reducing acclimation stress thereby facilitating adaptation to external conditions.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"25 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140100039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silver nitrate mediated improvement in micropropagation and amelioration of micro-morpho-anatomical structures in Oxystelma esculentum (L.f.) Sm.","authors":"","doi":"10.1007/s11627-024-10417-0","DOIUrl":"https://doi.org/10.1007/s11627-024-10417-0","url":null,"abstract":"<h3>Abstract</h3> <p>The present study highlights the effect of silver nitrate (AgNO₃) in the promotion of <em>in vitro</em> regeneration and morpho-structural growth and developments in <em>Oxystelma esculentum</em> (L.f.) Sm. The incorporation of AgNO₃ into the Murashige and Skoog (MS) medium resulted in a favorable enhancement of shoot proliferation. Among the different concentrations of AgNO₃ screened, 0.5 mg L⁻¹ AgNO₃ along with 0.5 mg L⁻¹ <em>meta</em>-Topolin (<em>m</em>T) and 0.25 mg L⁻¹ indole-3-acetic acid (IAA) stimulated the highest count, and length of shoots (143 shoots and 14.0 cm length) after 3 mo of incubation and the 3rd subculture. The <em>in vitro</em>–induced deformities in the tissues of the control stem and foliages were improved when the shoots were grown in AgNO₃-enriched medium. Silver nitrate increased the photosynthetic pigments and improved the formation of functional stomata. The AgNO₃-supplemented medium-derived shoots exhibited accelerated adventitious root induction (100% rhizogenesis, 21.0 roots per shoot) when the cut ends of shoots were treated with 200.0 mg L⁻¹ indole-3-butyric acid (IBA) for 5 min under greenhouse conditions. The incorporation of AgNO₃ with optimal plant growth regulators in the medium promoted overall growth. It prevented <em>in vitro</em>–induced morphological and anatomical anomalies in the leaf and stem, which considerably improved the greenhouse and <em>in vivo</em> survival of micropropagated plantlets.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"34 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140047687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of tissue culture regeneration system of Ficus tikoua","authors":"Xufei Li, Qianxia Li, Ying Cui, Tongfei Liu, Yanni Zhang","doi":"10.1007/s11627-024-10414-3","DOIUrl":"https://doi.org/10.1007/s11627-024-10414-3","url":null,"abstract":"<p><i>Ficus tikoua</i> Bureau is an ecologically functional perennial vine that belongs to the Moraceae family. Tissue culture is a common method for the rapid propagation of plants and is suitable for the rapid propagation of <i>F. tikoua</i>. The objective of the current study was to evaluate the process of <i>F. tikoua</i> organogenesis <i>in vitro</i> utilizing stem explants. An efficient tissue culture propagation method was developed by manipulating several plant growth regulators (PGRs) and their proportions, as well as evaluating alternative medium options for specific phases of differentiation. Explants were cultured on MS medium, supplemented with various thidiazuron (TDZ) concentrations with 6-benzylaminopurine (BA) and naphthaleneacetic acid (NAA), to investigate their efficacy during <i>in vitro</i> shoot organogenesis. The results demonstrated that the combination of 0.5 mg L⁻¹ BA, 1.0 mg L⁻¹ NAA, and 1.0 mg L⁻¹ TDZ was the most effective for callus formation (91.1%) and that the combination of 2.0 mg L⁻¹ BA and 0.5 mg L⁻¹ TDZ was the most suited for adventitious shoot induction (61.11%). The medium supplemented with 1.0 mg L⁻¹ BA, 1.0 mg L⁻¹ NAA, and 0.5 mg L⁻¹ TDZ yielded the highest bud multiplication number with a 6.05 proliferation factor. The cultured shoots rooted easily on half-strength MS medium containing 1.0 mg L⁻¹ IBA and 0.1 mg L⁻¹ NAA (68.52%). The subsequent regenerated plants were grown in a mixture of garden soil, perlite, and vermiculite in a ratio of 2:1:1, resulting in a survival rate of 86.17%.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"119 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140032521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of endogenous U6 promoters for expanding the CRISPR toolbox in Nicotiana tabacum","authors":"Pan Luo, Siyuan Li, Lulu Li, Yanyan Li, Ying Qiao, Xianzhi Wu, Meiling Wu, Zhihao Song, Yong Yang, Ji Feng, Xingchun Tang","doi":"10.1007/s11627-024-10418-z","DOIUrl":"https://doi.org/10.1007/s11627-024-10418-z","url":null,"abstract":"","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"48 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140047591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}