In Vitro Cellular & Developmental Biology - Plant最新文献

{"title":"Tissue culture and high-efficiency transformation in an apomictic initial variety of Paspalum notatum Flüggé","authors":"Rafael Narancio, Daniel Isenegger, Rafael Reyno, German Spangenberg, Marco Dalla-Rizza","doi":"10.1007/s11627-024-10434-z","DOIUrl":"https://doi.org/10.1007/s11627-024-10434-z","url":null,"abstract":"<p><i>Paspalum notatum</i> Flüggé is a subtropical grass native from the Americas and one of the main components of the subtropical South American grasslands. Although <i>P. notatum</i> exhibits a high forage productivity and persistence, some limitations are observed in the species, such as forage quality due to high lignin content. The development of efficient tissue culture and plant transformation protocols in cultivars for forage production are necessary to embrace novel genetic improvement through transgenesis and genome editing. Efficient regeneration and transformation systems for many plant species can be hampered by genotype dependency, which can only be determined through extensive laboratory testing and evaluation. In this study, various factors extracted from protocols have been evaluated to establish efficient somatic embryogenesis, plant regeneration, and genetic transformation in apomictic initial variety INIA Sepé. Optimal somatic embryogenesis and regeneration from explant sources, such as mature seeds and meristematic tissue derived from <i>in vitro</i> propagated tillers, ranged between 79 and 88%. Microprojectile bombardment of two plasmids encoding a constitutively regulated reporter gene and <i>nptII</i> selectable marker expression constructs showed an average transformation efficiency of 10.3% from meristematic tissue as an explant source. Furthermore, the constitutive promoters, <i>35S</i> and <i>ZmUbi</i>, were tested in <i>GFP</i> transient expression assays to evaluate optimal cassettes for transgene(s) expression. Translation of a wheat codon–optimized <i>Cas9</i> sequence using <i>Cas9:2A:tuGFP</i> fusion protein was also tested in protoplasts. The information generated on promoter activity and Cas9 translation, and the establishment of an efficient transformation system, provided useful tools for future work in genetic engineering and genome editing in <i>P. notatum</i> cv. INIA Sepé.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"22 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141503379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mannitol and sorbitol concentration optimization for effective Epipactis flava Seidenf. in vitro slow growth storage","authors":"Julaluk Linjikao, Phithak Inthima, Apinun Limmongkon, Anupan Kongbangkerd","doi":"10.1007/s11627-024-10437-w","DOIUrl":"https://doi.org/10.1007/s11627-024-10437-w","url":null,"abstract":"<p><i>Epipactis flava</i> Seidenf., a Thai rheophytic orchid, is endangered due to habitat destruction and climate change. <i>In vitro</i> conservation serves as an effective technique for the <i>ex situ</i> preservation of orchid diversity. This study investigated the <i>in vitro</i> storage of <i>E</i>. <i>flava</i> seedlings under slow growth conditions using Murashige and Skoog (MS) medium supplemented with either sorbitol or mannitol individually at concentrations of 1.0%, 2.0%, 3.0%, and 4.0% (<i>w/v</i>) for 24 wk without subculture. The storage medium added with 3.0% (<i>w/v</i>) sucrose was used as the control. After 24 wk of storage, the storage medium containing 2.0% mannitol provided the highest survival rate (97.6%) while the highest leaf formation (70.8%) was achieved with 3.0% sucrose. The addition of 1.0 to 2.0% sorbitol, 1.0% mannitol, and 3.0 to 4.0% mannitol showed the highest shoot bud formation (100%). At the end of 24 wk, the plantlets were transferred to fresh medium for an 8-wk growth recovery. The plantlets derived from the storage medium with 3.0% mannitol exhibited the highest survival rate (73.5%). Conversely, the highest number of shoot buds (9.9 shoot buds per plantlet) was found on plantlets derived from storage medium with 1.0% sorbitol while the highest number of shoots (4.8 shoots per plantlet) was achieved in plantlets derived from 1.0% mannitol. Consequently, these results suggested that the storage medium with 3.0% mannitol is optimal for conserving plantlets for up to 24 wk, and all plantlets could successfully produce shoot buds and shoots during <i>in vitro</i> recovery. Hence, this strategy has potential in the conservation and utilization of this endangered species.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"41 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141252995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of GRF-GIF genes enhances plant regeneration in cassava (Manihot esculenta)","authors":"Rosana Segatto, Gecele M. Paggi, Nigel J. Taylor","doi":"10.1007/s11627-024-10435-y","DOIUrl":"https://doi.org/10.1007/s11627-024-10435-y","url":null,"abstract":"<p>The <i>GROWTH-REGULATING FACTOR</i> (GRF) and its <i>INTERACTING FACTOR</i> (GIF) have been shown to stimulate regeneration of transgenic plants with studies reporting increased transformation efficiency in multiple species, including wheat, beet, and citrus. The present work evaluated the effects of overexpressing <i>GRF4-GIF1</i> and <i>GRF5</i> on the regeneration of transgenic plants in cassava (<i>Manihot esculenta</i> Crantz). Effects of <i>GRF4-GIF1</i> and <i>GRF5</i> sequences derived from <i>Vitis vinifera</i> and <i>Arabidopsis thaliana</i> were assessed by cloning expression cassettes under control of strong constitutive promoters. Friable embryogenic callus from cassava varieties 60444 and NASE 13 were transformed with <i>Agrobacterium tumefaciens</i> strains LBA4404 and LBA4404 THY-, and multiple independent transgenic plant lines recovered. Expression of the morphogenic genes did not enhance transformation efficiency, nor efficiency or timing of somatic embryo regeneration or whole plant recovery above the green fluorescent protein (GFP) control. Organogenesis experiments were carried out to observe effects of transgenic expression of these genes on morphogenesis from petiole, leaf-petiole, and stem explants. Results differed between the two genotypes evaluated. Expression of <i>Vitis vinifera GRF4-GIF1</i> was effective for stimulation of rapid organogenesis and shoot regeneration at 30 to 37% from leaf-petiole explants of cultivar 60444. In contrast, <i>Arabidopsis thaliana GRF5</i> was superior in stimulating shoot regeneration in cultivar NASE 13 with 40 to 50% of leaf-petiole explants regenerating shoots. In both cultivars, caulogenesis occurred rapidly within 3 to 4 wk culture on medium containing the cytokinin meta-topolin. Effects of overexpression of these morphogenic genes at the whole plant level were accessed by establishing plants in the greenhouse. <i>GRF4-GIF1</i> overexpression resulted in significantly shorter plants with increased leaf size and total leaf area while <i>AtGRF5</i> stimulated above average storage root weight.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"13 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141167676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel approach for direct shoot regeneration, anatomical characterization, and withanolides content in micropropagated plants of Withania somnifera (L.) Dunal—an important medicinal plant","authors":"Ganesan Mahendran, Laiq ur Rahman","doi":"10.1007/s11627-024-10428-x","DOIUrl":"https://doi.org/10.1007/s11627-024-10428-x","url":null,"abstract":"<p><i>Withania somnifera</i> (Ashwagandha) is a valuable therapeutic plant used in many Ayurvedic drugs marketed for health restoration. However, its commercial production faces challenges due to poor seed viability, low germination rates, and the absence of standardized propagation methods. Therefore, this investigation aimed to establish a fast and highly efficient method for direct shoot regeneration in <i>W. somnifera</i> by using the apical meristem perforation technique, which is a novel approach. Initially, rapid seed germination was standardized by soaking the seeds in distilled water for varying durations (0 to 48 h). The highest rates of shoot initiation (90.40 ± 0.51%) and mean number of direct shoot regenerations (16.20 ± 0.50 per explant) were achieved on MS basal medium fortified with meta-topolin (mT) at 3.0 mg L⁻¹. For optimal root development, shoots (3 to 4 cm) were cultured on half-strength Murashige and Skoog (MS) medium with 3.0 mg L⁻¹ indole-3-butyric acid (IBA). Subsequently, well-rooted seedlings (90% survival rate) were acclimatized under glasshouse conditions in pots filled with a mixture of sandy loam and vermicompost (4:1). Anatomical examination of leaves and stems from micropropagated and acclimatized glasshouse plants revealed well-developed cuticles, differentiated mesophylls, and vascular bundles. Furthermore, the accumulations of withaferin A (1.26 ± 0.12 mg g⁻¹), withanone (1.39 ± 0.18 mg g⁻¹), and withanoside V (0.11 ± 0.04 mg g⁻¹) in greenhouse-acclimated plant leaves were 1.88, 2.28, and 2.30 times higher, respectively, than in tissue culture samples (0.52 ± 0.07 mg g⁻¹, 0.73 ± 0.02 mg g⁻¹, and 0.05 ± 0.02 mg g⁻¹). In conclusion, the described procedure offered an effective and straightforward method for large-scale production of <i>W. somnifera</i> plants for commercial metabolites.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"41 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140929872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low temperature and dark culture inhibited explant browning and promoted in vitro regeneration of Juglans mandshurica","authors":"Chunping Liu, Xinrui Fan, Baiting Qin, Aonan Li, Lijie Zhang","doi":"10.1007/s11627-024-10433-0","DOIUrl":"https://doi.org/10.1007/s11627-024-10433-0","url":null,"abstract":"<p>\u0000<i>Juglans mandshurica</i> is an important component of Korean pine mixed and secondary forests in Northeast China and is also a precious timber and economic species. In this study, plant regeneration of <i>J. mandshurica</i> has been established using stems with bud and mature zygotic embryos as explants. The results showed that placing the stems with buds in low-temperature conditions (0 to 4°C) and in darkness for 5 d inhibited the browning of the explants and promoted bud sprouting; Na₂S₂O₃ also inhibited the browning of mature zygotic embryos significantly. The suitable basal media and hormones for bud sprouting and zygotic embryo germination were (1) WPM medium (woody plant medium) containing 2.0 mg·L⁻¹ 6-benzylaminopurine (6-BA) and 0.01 mg·L⁻¹ 3-indolebutyric acid (IBA) and (2) DKW medium (Driver and Kuniyuki medium) containing 2.5 mg·L⁻¹ 6-BA and 0.1 mg·L⁻¹ IBA. The suitable basal medium and hormone for rooting of shoots from buds was 1/2 Murashige and Skoog (MS) medium (the macro-elements were half-strength of MS medium) supplemented with 1.0 mg·L⁻¹ IBA, and the suitable basal medium and hormone for rooting of seedlings derived from zygotic embryo was 1/2 MS medium containing 1.0 mg·L⁻¹ IBA, and DKW medium with 2.5 mg·L⁻¹ 6-BA, 0.1 mg·L⁻¹ IBA, 0.2 mg·L⁻¹ gibberellin A3 (GA₃), and 0.2 g·L⁻¹ activated carbon (AC). About 95% of the rooted plantlets survived after acclimation and transplantation. These results provided a foundation for the scale propagation to meet the demands of forestry production of the species.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"63 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140887776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shoot propagation, regeneration, and callus induction and differentiation, of Axonopus compressus (Swartz) Beauv","authors":"Yuping Xiong, Zhenpeng Wei, Junyu Liu, Jianrong Li, Shuguang Jian, Xinhua Zhang, Yuan Li, Zhan Bian, Kunlin Wu, Songjun Zeng, Jaime A. Teixeira da Silva, Guohua Ma","doi":"10.1007/s11627-024-10432-1","DOIUrl":"https://doi.org/10.1007/s11627-024-10432-1","url":null,"abstract":"<p><i>Axonopus compressus</i> (Swartz) Beauv., a perennial herb in the Poaceae that has been introduced to a number of tropical and subtropical countries and regions, can serve as a lawn ground cover while its leaves can be developed as a bioenergy resource. It also displays some resistance to heavy metals, allowing it to be planted in areas and urban green spaces polluted by heavy metals. <i>A. compressus</i> is also used as a traditional Chinese medicine. This study is the first report on tissue culture of <i>A. compressus</i>. Stem explants induced shoot clusters on 6-benzyladenine (BA)-containing Murashige and Skoog (MS) medium, with 2.0 mg L^–1 BA inducing a shoot proliferation coefficient (SPC) of 10.89 within 30 d, while MS medium containing 2.0 mg L^–1 BA and 0.1 mg L^–1 α-naphthaleneacetic acid (NAA) amplified SPC to 12.88 within 30 d. SPC on MS medium supplemented with kinetin (KIN) or thidiazuron (TDZ) was not as high as on medium with BA and never exceeded 3.57 within 30 d. <i>A. compressus</i> formed adventitious roots easily, within 15 d, most efficiently on ½MS medium supplemented with 0.1 to 0.5 mg L^–1 IAA, NAA, or IBA, or even on auxin-free medium. Resulting plantlets displayed a high survival rate (&gt; 98%) when transplanted to a substrate containing humus, or humus mixed with vermiculite or sand. Callus was also successfully induced from leaf sheaths on MS medium containing 1.0 mg L^–1 2,4-dichlorophenoxyacetic acid, even more so when 1.15 g L^–1 <span>l</span>-proline was added. Induced callus was able to differentiate into adventitious shoots on MS medium with 0.1 to 0.5 mg L^–1 KIN or BA. An efficient system of shoot proliferation, callus induction, and regeneration in <i>A. compressus</i> has been established.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"27 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140827836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plantlet regeneration via somatic embryogenesis and changes in endogenous hormone content of Rosa ‘John F. Kennedy’","authors":"Li Du, Xiaoling Kang, Zhongfeng Zhu, Yu Ma, Haoran Guo, Jingman Li, Chuanyu Ding","doi":"10.1007/s11627-024-10426-z","DOIUrl":"https://doi.org/10.1007/s11627-024-10426-z","url":null,"abstract":"<p>This study describes a plantlet regeneration protocol of somatic embryos in <i>Rosa</i> ‘John F. Kennedy’ (hybrid tea rose). Different somatic embryo sizes exhibited significant differences in the single bud (SB type) regeneration rate and multiple bud (MB type) regeneration rate. The highest single bud (SB type) regeneration rate (27.10%) was obtained from the large size (4 mm × 5 mm). The multiple bud regeneration rate was highest at 39.60% for the medium size (3 mm × 4 mm). Changes in the endogenous hormone content and ratios of various types of embryogenic cultures were clearly diverse: higher contents of abscisic acid (ABA) and indole-3-acetic acid (IAA) occurred in the SPC explant (single-piece cotyledonary somatic embryo) with a regenerated single bud (SB type). In a MW-type somatic embryo (milky-white single-piece-cotyledon explant), the gibberellic acid (GA₃)/ABA ratio was the highest (1.807), and the IAA/GA₃ ratio was the lowest (0.902). However, the highest ratios of IAA/GA₃ (6.159) and the lowest ratios of GA₃/ABA (0.383) appeared in SB-type cultures. Additionally, the highest IAA/ABA ratios (6.535) and higher ratios of GA₃/ABA (1.729) were found in MB-type cultures. This indicated that ways to regulate plant cell totipotency in <i>Rosa</i> ‘John F. Kennedy’ somatic embryos differed between single bud (SB type) regeneration and multiple bud (MB type) regeneration. Finally, this study classified and summarized common intermediate materials in <i>in vitro</i> culture based on morphological characteristics and plantlet regeneration pathways.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140827674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Droplet vitrification: a lifeline for long-term conservation of threatened species Garcinia indica","authors":"Vartika Srivastava, Bart Panis, Anuradha Agrawal","doi":"10.1007/s11627-024-10423-2","DOIUrl":"https://doi.org/10.1007/s11627-024-10423-2","url":null,"abstract":"<p>Cryopreservation is a promising technique for the <i>ex situ</i> long-term conservation of plant biodiversity particularly of species that are not amenable to seed bank conservation. <i>Garcinia indica</i> (Thouars) Choisy is a species endemic to the biodiversity hotspot of the Western Ghats of India. Conservation of this species is difficult as it produces recalcitrant seeds, and therefore, it can only be conserved in the field genebanks or in their natural habitats. This high-value fruit tree species is listed as vulnerable due to the rapid loss of the natural population, and therefore, the conservation of its genetic diversity is imperative. This study described the first successful cryopreservation protocol for <i>G. indica</i> through a modified droplet vitrification technique using <i>in vitro</i>–derived shoots conserved in the <i>In Vitro</i> Gene Bank of the Indian Council of Agricultural Research - National Bureau of Plant Genetic Resources (ICAR-NBPGR), New Delhi. Among vitrification (V) and droplet vitrification (DV) techniques, only the DV technique yielded explant regeneration after cryopreservation. Apical shoots (2 mm long) collected from 24-wk-old explants (IC638183), without any pre-culture, showed maximum regeneration (51.76%) on Murashige and Skoog (MS) medium supplemented with 2.22 μM 6-benzylaminopurine (BAP). The regenerated shoots were hardened in a mist chamber successfully (85% survival), which is essential for the future restoration of the species in the field. With genotypic-dependent variation, this protocol was applicable to the other three accessions (IC638184, IC638185, and IC638186) with an average of 43.7% regeneration after cryopreservation, which can be implemented for the effective conservation of <i>G. indica</i> germplasm.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"29 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140800309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scratching the surface: The in vitro research that will be critical for conserving exceptional plants to scale","authors":"Valerie C. Pence, Emily Beckman Bruns","doi":"10.1007/s11627-023-10405-w","DOIUrl":"https://doi.org/10.1007/s11627-023-10405-w","url":null,"abstract":"<p>The conservation of threatened exceptional plants, which cannot be conserved by seed banking, requires <i>in vitro</i> technologies for many of the approaches needed for their long-term <i>ex situ</i> conservation. This study evaluated the current <i>in vitro</i> plant literature, as represented in Web of Science, to determine its taxonomic overlap with the families and genera of the 775 species currently listed as exceptional. Web of Science was searched using the terms micropropagation, somatic embryogenesis, zygotic embryo, and cryopreservation, and the target genera and families were identified in the more than 19,000 articles evaluated. There were five families with significant overlap between the <i>in vitro</i> literature and exceptional species: Fabaceae, Asteraceae, Orchidaceae, Arecaceae, and Rutaceae. However, there was less overlap at the level of genus, with <i>Citrus, Coffea,</i> and <i>Quercus</i> having the most articles<i>.</i> Significant gaps were also found, with 14 exceptional families and half of the exceptional genera having no representation in the Web of Science search results. The 20 exceptional species with the most articles were all economically important species, and these had 343 threatened congeners that could be prioritized for research. A highly important group of exceptional plants that was significantly under-represented in the literature was tropical woody species, which form the backbone of the diversity of the world’s threatened rainforests. Overall, there are areas of strength upon which to build future work, but significant gaps where research should be prioritized for effectively conserving exceptional plants.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"54 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140811548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification, characterization, and expression analysis of the MAPK gene family in Nardostachys jatamansi (D. Don) DC","authors":"Shubham Joshi, Jhilmil Nath, Rohit Joshi","doi":"10.1007/s11627-024-10430-3","DOIUrl":"https://doi.org/10.1007/s11627-024-10430-3","url":null,"abstract":"<p>Mitogen-activated protein kinase (<i>MAPK</i>) cascade is a widely distributed signaling pathway, which is involved in growth, development, and stress responses in plants. <i>Nardostachys jatamansi</i> (Caprifoliaceae) is a perennial, high-altitude Himalayan medicinal plant, which experiences varying environmental fluctuations throughout its life span. However, how the fluctuating environment is regulated <i>via</i> <i>MAPKs</i> in high-altitude medicinal plants, such as <i>Nardostachys jatamansi</i> as well as in other members of the Caprifoliaceae family, is poorly understood. In the present study, <i>Lonicera japonica</i> Thunb. (Caprifoliacae) was used as a model for genome-wide understanding of <i>MAPKs</i> in the Caprifoliaceae family; further, <i>Nardostachys jatamansi</i> was used for expression profiling of <i>MAPKs</i> under various growth stages and abiotic stress conditions. Twenty <i>LjMAPKs</i> and 20 <i>NjMAPKs</i> were identified from the <i>Lonicera japonica</i> genome and <i>Nardostachys jatamansi</i> transcriptome database. The identification, characterization, subcellular localization, phylogenetic analysis, chromosomal localization, gene structure, synteny, and cis-acting elements of the <i>LjMAPKs</i> and <i>NjMAPKs</i> gene family were evaluated using <i>in silico</i> approaches. Phylogenetic and other <i>in silico</i> investigations showed maximum similarity between <i>LjMAPKs</i> and <i>NjMAPKs</i> protein sequences. Cis-regulatory elements identified in the promoter region of <i>LjMAPKs</i> included development, light, phytohormone, and stress-responsive elements. Tissue-specific expression profiling showed ubiquitous expression of <i>MAPKs</i> in both <i>L. japonica</i> and <i>N. jatamansi</i>. However, differential transcript abundance was observed in leaf and root tissues of <i>NjMAPKs</i> under varying abiotic stress conditions. The present study’s findings provided a fundamental understanding of <i>MAPKs</i> in <i>L. japonica</i> and <i>N. jatamansi</i> and may contribute towards a better understanding of the molecular mechanisms regulating plant development and abiotic stress response.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"41 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140637303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}