In Vitro Cellular & Developmental Biology - Plant最新文献

{"title":"Establishment of tissue culture regeneration system for an important medicinal plant: Codonopsis pilosula","authors":"Yuan Xu, Xuehong Qi, Wenhui Zhu, Xinxin Zhang, Bingqing Tao, Xingzheng Wang, Wei Wang, Ning Yang","doi":"10.1007/s11627-025-10588-4","DOIUrl":"https://doi.org/10.1007/s11627-025-10588-4","url":null,"abstract":"","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"62 1","pages":"98-113"},"PeriodicalIF":0.0,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147331140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scalable production of Actinidia arguta adventitious roots in air-lift bioreactors and their antibacterial efficacy against Staphylococcus aureus","authors":"Wen Jiang, Ximei Zong, Shunqing An, Jing-Run Xiu, Shuo Yu, Kexin Zhang, Yue‐Jun Hao, Meiyu Jin","doi":"10.1007/s11627-025-10558-w","DOIUrl":"https://doi.org/10.1007/s11627-025-10558-w","url":null,"abstract":"","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"61 6","pages":"1215-1227"},"PeriodicalIF":0.0,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147331913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis reveals the mechanism of silicon-regulated reversion of hyperhydricity in Dendrobium officinale","authors":"Hongli Shang, Xi Huang, Xinyu Zhang, Zi‐Qi Zheng, Quan Yang, Hongyang Gao","doi":"10.1007/s11627-025-10553-1","DOIUrl":"https://doi.org/10.1007/s11627-025-10553-1","url":null,"abstract":"","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"61 2","pages":"443-456"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147332435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of autoallododecaploid barnyard grass (Echinochloa crus-galli L.) induced by in vitro colchicine treatment","authors":"Keyi Liu, Xiaoling Zhou, Wei Wang, Xiaoxiao Wu, Ziwei Zhao, Xiaolu Yang, Ziyi Feng, Pincang Lv, Jie Wang, Wangwen Zhu, Xianhua Zhang, Yuchi He, Detian Cai, Zhaojian Song","doi":"10.1007/s11627-024-10461-w","DOIUrl":"https://doi.org/10.1007/s11627-024-10461-w","url":null,"abstract":"<p>A protocol for <i>in vitro</i> induction of autoallododecaploid <i>Echinochloa crus-galli</i> with colchicine treatment was established. Colchicine concentration and exposure time had significant effects on the callus survival rate, shoot differentiation rate, and chromosome doubling rate. With the increase of colchicine concentration and the extension of treatment time, the callus survival rate and shoot differentiation rate both decreased. Treatment with 0.05% colchicine for 48 hr was the most effective condition for polyploidization, yielding 42.99% dodecaploids. The authenticity of the induced dodecaploid was confirmed by nuclear DNA content, chromosome number, and stomatal and morphological characteristics. Dodecaploid plants showed the obvious “giant” effects of polyploid organs, as well as significantly reduced seed set rate. The concentrations of calcium, iron, most free amino acids, and total free amino acids in dodecaploid plants were significantly higher than those in hexaploid plants. The results showed that the chromosome of allohexaploid <i>E. crus-galli</i> could be redoubled. The dodecaploid <i>E. crus-galli</i> could still grow normally and showed advantages associated with polyploidization, such as enlarged organs and increased nutrient concentrations. The study enriched the germplasm resources of barnyard grass and laid a foundation for the utilization and theoretical research of <i>E. crus-galli</i> in the future.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"46 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142249422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of a Malus baccata (L.) Borkh WRKY transcription factor gene MbWRKY65 increased the tolerance to cold and drought in transgenic tomato","authors":"Chunwen Yu, Anqi Yao, Xingguo Li, Wenhui Li, Ruina Gao, Yuqing Feng, Zhuxuan Li, Xinxin Guo, Lihua Zhang, Deguo Han","doi":"10.1007/s11627-024-10458-5","DOIUrl":"https://doi.org/10.1007/s11627-024-10458-5","url":null,"abstract":"<p>Plants exposed to harsh external environments such as cold and drought display stunted growth and delayed development. WRKY proteins can bind to the cis-acting element W-box to activate or repress transcription of downstream target genes to regulate responses, including different abiotic and biotic stresses. In this experiment, <i>Malus baccata</i> (L.) Borkh was used to isolate the <i>MbWRKY65</i> gene to study its cold and drought resistance functions. The outcomes demonstrated that <i>MbWRKY65</i> is most similar to the apple <i>MdWRKY65</i> protein. Its location in the nucleus is shown by subcellular localization data, which is in line with its role as a transcription factor. <i>MbWRKY65</i> was expressed richer in roots and older leaves in response to cold and drought treatments. The transgenic tomato showed considerably reduced contents of malondialdehyde (MDA), hydrogen peroxide (H₂O₂), superoxide anion radical (O₂⁻), and relative conductivity during cold and drought stress in contrast to wild-type (WT) and unloaded lines (UL), but greater contents of proline and chlorophyll, as well as higher activity of catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD). Stress-related genes (<i>LeCBF1</i>, <i>LeABI3</i>, <i>LeABF4</i>, <i>LeCBF3</i>, <i>LeNCED1</i>, and <i>LeDREB1</i>) in transgenic tomato plants were positively regulated under conditions of cold and drought, and in contrast to the WT and UL lines, the transgenic tomato showed a considerably greater expression level. In conclusion, <i>MbWRKY65</i> actively contributes to plant tolerance to cold and drought.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"191 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142249423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of plant growth regulators on in vitro regeneration of Saposhnikovia divaricata (Turcz. ex Ledeb.) Schischk","authors":"Tatiana Zheleznichenko, Tatyana Elisafenko, Bayarma Zhigmittsyrenova, Maksim Kazakov, Vasilii Taraskin","doi":"10.1007/s11627-024-10462-9","DOIUrl":"https://doi.org/10.1007/s11627-024-10462-9","url":null,"abstract":"<p><i>Saposhnikovia</i> <i>divaricata (Turcz. ex Ledeb.) Schischk</i> is a medicinal plant with a broad spectrum of pharmacological activities, which are attributed to the presence of various bioactive compounds, including chromones. The uncontrolled harvesting of this plant has resulted in the depletion of its natural resources. The ability of <i>S. divaricata</i> to regenerate <i>in vitro</i> was studied. Aseptic culture was established by sterilizing seeds and germinating them, both with and without the pericarp. Adventitious shoot formation through direct morphogenesis was achieved on Murashige and Skoog medium in the presence of different cytokinins: benzylaminopurine, <i>meta</i>-topolin, and thidiazuron, at concentrations of 0.1 µM, 0.5 µM, and 1.0 µM. It has been determined that the most preferable explants were the cotyledonary nodes of aseptic seedlings, as they enable the rapid production of 1.4 ± 0.5 to 10.2 ± 2.0 microshoots per explant, depending on the type and concentration of the cytokinin used. Successful regeneration also occurred from the first true leaves of seedlings <i>in vitro</i>. Microshoots were formed directly from the explant tissues, with their quantity ranging from 1.7 ± 0.7 to 4.4 ± 0.9. The highest number of microshoots was induced by thidiazuron, but after cultivation with <i>meta</i>-topolin, the regenerated plants showed the highest frequency of rooting, ranging from 66 to 97%. Somatic embryoids were obtained when the first true leaves were cultivated with 2,4-dichlorophenoxyacetic acid (10.0 µM). Morphogenesis occurred <i>via</i> the indirect pathway (through the callus formation stage), accompanied by the formation of up to 24.2 ± 6.1 somatic embryoids, with a rooting frequency reaching 96%. These studies provide a preliminary basis for developing a protocol for the clonal micropropagation of <i>S. divaricata.</i></p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"7 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro regeneration protocol for endemic Campanula leblebicii Yıldırım","authors":"Yasemin Kartal, Başar Sevindik, Ademi Fahri Pirhan","doi":"10.1007/s11627-024-10447-8","DOIUrl":"https://doi.org/10.1007/s11627-024-10447-8","url":null,"abstract":"<p><i>Campanula leblebicii</i>, discovered by Yıldırım, is found only in the village of Ovacık in the Kemalpaşa district of İzmir province, and it is one of the valuable endemic species for Turkey flora. Petiole and leaf explants were cultured on full-strength Murashige and Skoog (MS) medium containing 30.0 g L⁻¹ sucrose; 4.0 g L⁻¹ Gelrite; 0.3 mg L⁻¹ 1-naphthaleneacetic acid (NAA); 0, 0.5, 1.0, 2.0, or 3.0 mg L⁻¹ zeatin (ZEA); and 0, 0.5, 1.0, 2.0, or 3.0 mg L⁻¹ 6-benzyladenine (BA) for callus initiation. To obtain shoot formation, callus was transferred to full-strength MS medium containing 0.3 mg L⁻¹ NAA; 0, 0.5, 1.0, 2.0, or 3.0 mg L⁻¹ gibberellic acid (GA₃); and 0, 0.5, 1.0, 2.0, 3.0 mg L⁻¹ BA. Efficient callus (93.33%) and shoot formation (73.33) were observed from petiole explants cultured on MS medium containing 0.3 mg L⁻¹ NAA and 1.0 mg L⁻¹ BA. Rooting was obtained spontaneously in all shoot initiation medium, but efficient rooting occurred on MS medium supplemented with 0.3 mg L⁻¹ NAA and 1.0 mg L⁻¹ BA. Plants were acclimatized to the pots including sterile peat and perlite (1:1, v/v), and, in total, 12 plants were acclimatized from one genotype.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"18 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of an efficient micropropagation protocol for Persicaria glabra by the synergistic effect of ancymidol and benzyladenine","authors":"Kakkerintavida Arsha, E. A. Siril","doi":"10.1007/s11627-024-10455-8","DOIUrl":"https://doi.org/10.1007/s11627-024-10455-8","url":null,"abstract":"<p>An efficient <i>in vitro</i> propagation protocol for a medicinal plant, <i>Persicaria glabra</i> (Willd.) M. Gomez, has been established. Nodal explants were cultured <i>in vitro</i> on agar-gelled (0.8%) Murashige and Skoog (MS) medium containing different concentrations of cytokinins, namely N⁶-benzyladenine (BA), 6-(γ,γ-dimethylallylamino)purine (2-iP), or kinetin (KIN). BA (2.5 µM) was found to be the most effective cytokinin for axillary bud proliferation. A growth retardant ancymidol (ANC) was combined with 2.5 µM BA to enhance the multiple shoot induction and ensure the overall health of micro shoots. The nodal explants taken from micro shoots developed in 2.5 µM BA were subcultured on agar-gelled MS medium supplemented with different concentrations of ANC along with 2.5 µM BA. Among the different concentrations tested, 4.0 µM ANC combined with 2.5 µM BA produced a maximum number of multiple shoots (13 shoots per explant). A progressive decline in the shoot height was observed with increasing concentrations of ANC and significantly reduced shoot length (0.5 cm) at a higher concentration (12.0 µM ANC). Reduced leaf area and more chlorophyll were noticed in ANC-treated plants than in BA-alone-treated plants. This facilitated better growth and development of robust micro shoots. Well-developed micro shoots were excised from 4.0 µM ANC along with 2.5 µM BA and subjected to <i>ex vitro</i> rooting experiments using different concentrations of auxins, including α-naphthaleneacetic acid (NAA) or indole-3-butyric acid (IBA). The maximum number of roots (46.33 roots per shoot) and root length (14.53 cm) were observed in 0.5 mM IBA with 89.11% of the response. The rooted plants were successfully acclimatized in field conditions with 100% survival. The genetic fidelity analysis of <i>in vitro</i>–raised plants was tested using inter simple sequence repeat (ISSR) markers, and they showed genetic monomorphism with the mother plant. The present study suggests that adding ANC and BA would enhance the multiple shoots induction and high rate of acclimatization of <i>P. glabra</i> cultures. The addition of ANC resulted in a 2.6-fold increase in shoot number and avoided undesirable elongation of shoots. Healthy shoots, thus, produced in ANC combined with BA-added medium doubled the survival of plants during acclimatization over a BA-alone medium and can be used in many other plant species that have the problem of developing shoots of an unhealthy nature coupled with abnormal internodal elongations.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"93 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro propagation of triploid Toxicodendron vernicifluum (Stokes) F. A. Barkl","authors":"Xiaolin Zhang, Jiayu Feng, Yiran Luo, Xiaomeng Duan, Chengzhong He, Dan Zong","doi":"10.1007/s11627-024-10452-x","DOIUrl":"https://doi.org/10.1007/s11627-024-10452-x","url":null,"abstract":"<p>Triploid lacquer trees have a higher yield of raw lacquer and greater stress resistance; however, it is challenging to develop efficient and rapid seedling culture techniques because of the influence of metabolites with raw lacquer. In this study, the stem segments of a triploid lacquer tree were used to investigate the effects of explant sterilization treatment, different combinations of exogenous hormone concentrations on the induction of axillary buds, seedling development, and rooting, as well as transplanting substrate ratios. The results showed that the optimal combination of disinfection treatment for triploid <i>Toxicodendron vernicifluum</i> (Stokes) F. A. Barkl explants involves 75% alcohol immersion for 10 s, followed by 2.0% benzalkonium chloride treatment for 1 min and 0.1% HgCl₂ treatment for 4 min. The suitable medium for axillary bud induction was Murashige and Skoog (MS) containing 0.1 mg•L⁻¹ zeatin (ZT) and 0.1 mg•L⁻¹ 6-benzylaminopurine (6-BA), resulting in a high induction rate of 95.0%. Use of 1/2 MS supplemented with 0.5 mg•L⁻¹ 6-BA and 0.3 mg•L⁻¹ 2,4-dichlorophenoxyacetic acid (2,4-D) was the most effective for axillary bud proliferation. The combination of 1/2 MS containing 0.1 mg•L⁻¹ 6-BA, 0.5 mg•L⁻¹ 2,4-D, 0.5 mg•L⁻¹ indole-3-butyric acid (IBA), and 0.2% activated carbon (AC) was an optimum for hardening-off. With woody plant medium (WPM) supplemented with 0.5 mg•L⁻¹ naphthaleneacetic acid (NAA) and 0.5 mg•L⁻¹ 2,4-D and 0.5 g•L⁻¹ AC as a rooting medium, the rooting rate was 67.8%. Also, the appropriate transplanting substrate ratio for triploid <i>Toxicodendron vernicifluum</i> (Stokes) F. A. Barkl was grass charcoal:laterite:humus = 1:1:1. Collectively, the results provided the technical assurance for seedling scale multiplication and propagation, facilitating its application in production.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"273 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a high-frequency in vitro regeneration system in Indian lotus (Nelumbo nucifera Gaertn.)","authors":"Rita Verma, Ankita Yadav, Rajan Kumar Gupta, Indraneel Sanyal","doi":"10.1007/s11627-024-10453-w","DOIUrl":"https://doi.org/10.1007/s11627-024-10453-w","url":null,"abstract":"<p>The Indian lotus (<i>Nelumbo nucifera</i> Gaertn.) is a popular ornamental plant and a source of traditional herbal medicine. Its various parts are widely used in the pharmaceutical, cosmeceutical, and nutraceutical industries. The recent study aimed to develop a high-frequency <i>in vitro</i> regeneration system in Indian lotus. This study utilizes the pink lotus cultivar from the Botanical Garden of CSIR-NBRI Lucknow. The study was successfully achieved through direct and indirect methods using different plant growth regulators (PGRs). A direct regeneration system was established using explants shoot apical meristem and plumule cultured on SIM supplemented with 17 combinations and concentrations of BAP and NAA. Both explants produced the highest number of shoots with a combination of 4.44 μM BAP and 0.55 μM NAA. The highest number of shoots per explant 25 ± 1.0 was developed from the shoot apical meristem, while the plumule explant developed 16.3 ± 0.5 shoots per explant. Thereafter, the plantlets were transferred to LRIM, which contained 17 combinations and concentrations of NAA or IBA and BAP. The maximum number of roots, per explant 23.6 ± 0.5, was developed from shoot apical meristem using 2.22 μM NAA and 0.54 μM BAP. The highest number of roots, per explant 23 ± 1.0, was developed from the plumule using 4.44 μM IBA. Indirect somatic embryogenesis has been established through callus culture. The leaf segments were cultured onto a callus induction medium supplemented with ten combinations of 2,4-D and BAP. The high-frequency callus formation 24.33 ± 0.5 was obtained with a 5.0 μM 2,4-D and 1.0 μM BAP combination. All developmental stages at the proembryo, globular, heart, torpedo, and mature embryos were formed on concentrations of 2,4-D and BAP. After inducing shoot and root growth, well-developed plantlets were transferred to the greenhouse, resulting in a success rate of 18.47%.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"54 9 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}