越橘胼胝体培养产生花青素的替代方法及产生花青素的胼胝体的超微结构

IF 2.2 3区 生物学 Q4 CELL BIOLOGY
Havva Karahan, Elif Onan, Hatice Çölgeçen
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引用次数: 0

摘要

本研究的目的是尝试增加胼胝体中花青素的产生量,并研究越橘(Vaccinium arctostaphylos L.)叶片、顶端分生组织和节的外植体中正在发育的胼胝体细胞的超微结构。为了产生胼胝体,将灭菌的外植体播种在含有不同浓度吲哚乙酸或玉米素的木本植物培养基(WPM)中。在含有 2.0 mg L-1 吲哚乙酸的培养基上培养的顶端分生组织外植体获得的含花青素最多的胼胝体。用不同浓度的蔗糖对含 2.0 mg L-1 吲哚乙酸的培养基进行改良,以提高花青素产量和胼胝体生物量。在含 50.0 g L-1 蔗糖的培养基上培养的顶端分生组织外植体产生的胼胝体最好,在 525 纳米绿色和 610 纳米红色波长的荧光显微镜下观察,胼胝体呈粉红色。胼胝体具有自发荧光。制备过程结束后,使用透射电子显微镜(TEM)检查了在含 50.0 g L-1 蔗糖的培养基中生长的粉色和黄色胼胝体的细胞超微结构。由黄色胼胝体发育而成的细胞被称为 1 型细胞,其形状为椭圆形或长角形。1 型细胞有一个大液泡。液泡周围有电子致密的小泡,附着在营养体的内表面,液泡中可见类似花青素的液泡包涵体。细胞质和细胞器被困在液泡和细胞壁之间。据观察,由粉红色胼胝体发育而成的细胞(称为 2 型)通常呈椭圆形。与 1 型细胞相似,它也有一个大液泡;细胞质被困在细胞壁和液泡之间,细胞间的空隙很少。一些液泡内有花青素泡状包涵体。细胞质小泡和液泡之间有类似大自噬的融合。这些观察结果表明,旱金莲的花青素生产细胞利用液泡运输法将花青素运输到液泡中。目前的研究首次填补了文献空白,对今后的研究具有极其重要的参考价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The alternative approaches to anthocyanin production by callus culture of Vaccinium arctostaphylos L. and the ultrastructure of anthocyanin-producing callus

The alternative approaches to anthocyanin production by callus culture of Vaccinium arctostaphylos L. and the ultrastructure of anthocyanin-producing callus

In this study, the aim was to try to increase the anthocyanin-producing in callus and to investigate the ultrastructure of the developing callus cells of leaf, apical meristem, and node explants of Vaccinium arctostaphylos L. To generate callus, sterilized explants were seeded in Woody Plant Medium (WPM) containing different concentrations of indoleacetic acid or zeatin. The most calluses containing anthocyanin were obtained from the apical meristem explant cultured on medium containing 2.0 mg L−1 indoleacetic acid. The medium containing 2.0 mg L−1 indoleacetic acid was modified with different sucrose concentrations and were prepared to increase anthocyanin production and callus biomass. The best callus production was obtained with apical meristem explants cultured on medium containing 50.0 g L−1 sucrose and was observed as a pink color callus when examined under a fluorescence microscope with 525-nm green and 610-nm red wavelengths. The callus had autofluorescent. The cell ultrastructures of the pink- and yellow-colored callus growing in medium containing 50.0 g L−1 sucrose were examined using a transmission electron microscope (TEM) after the preparation process. Cells that developed from yellow callus and were called type 1 had an elliptical or long, angular shape. Type 1 cells had a large vacuole. There were electron-dense vesicles around the vacuoles attached to the inner surface of the tonoplast, and anthocyanic vacuolar inclusion–like inclusions were seen in the vacuole. The cytoplasm and organelles were trapped between the vacuole and the cell wall. It was observed that cells developed from pink color callus (called type 2) often had an elliptical shape. Similar to type 1 cells, it has a large vacuole; its cytoplasm is trapped between the cell wall and the vacuole with very few intercellular spaces. Some of the vesicles had an anthocyanic vacuolar inclusion appearance within the vacuole. A macroautophagy-like fusion was placed between the cytoplasmic vesicles and the vacuole. These observations showed that the anthocyanin-producing cells of the V. arctostaphylos L. transported anthocyanins to the vacuole using the vesicular transport method. The current research is the first study to fill the gap in the literature, so it is extremely important as a source for future studies.

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来源期刊
CiteScore
5.00
自引率
7.70%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Founded in 1965, In Vitro Cellular & Developmental Biology - Plant is the only journal devoted solely to worldwide coverage of in vitro biology in plants. Its high-caliber original research and reviews make it required reading for anyone who needs comprehensive coverage of the latest developments and state-of-the-art research in plant cell and tissue culture and biotechnology from around the world.
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