开发菡萏(Nelumbo nucifera Gaertn.)高频离体再生系统

IF 2.2 3区 生物学 Q4 CELL BIOLOGY
Rita Verma, Ankita Yadav, Rajan Kumar Gupta, Indraneel Sanyal
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引用次数: 0

摘要

菡萏(Nelumbo nucifera Gaertn.)它的各个部分被广泛用于制药、药妆和保健品行业。最近的研究旨在开发菡萏的高频体外再生系统。这项研究利用了来自勒克瑙 CSIR-NBRI 植物园的粉红莲品种。该研究通过使用不同的植物生长调节剂(PGRs)的直接和间接方法成功实现。直接再生系统是利用芽顶端分生组织和小叶在添加了 17 种组合和浓度的 BAP 和 NAA 的 SIM 上培养的外植体建立的。在 4.44 μM BAP 和 0.55 μM NAA 的组合下,两种外植体产生的芽数量最多。芽顶端分生组织每个外植体产生的芽数最多,为 25 ± 1.0 个,而胚珠外植体每个外植体产生的芽数为 16.3 ± 0.5 个。之后,将小植株转移到含有 17 种 NAA 或 IBA 和 BAP 组合和浓度的 LRIM 中。使用 2.22 μM NAA 和 0.54 μM BAP 时,每个外植体的根数量最多,为 23.6 ± 0.5。使用 4.44 μM IBA,从胚珠中长出的根数最多,为每个外植体 23 ± 1.0 条。通过胼胝体培养建立了间接体细胞胚胎发生。将叶片培养在胼胝体诱导培养基上,培养基中添加 2,4-D 和 BAP 的十种组合。5.0 μM 2,4-D 和 1.0 μM BAP 的组合获得了 24.33 ± 0.5 的高频率胼胝体形成。原胚、球胚、心胚、鱼雷胚和成熟胚的所有发育阶段均在 2,4-D 和 BAP 的浓度下形成。在诱导芽和根的生长后,将发育良好的小苗移入温室,成功率为 18.47%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Development of a high-frequency in vitro regeneration system in Indian lotus (Nelumbo nucifera Gaertn.)

Development of a high-frequency in vitro regeneration system in Indian lotus (Nelumbo nucifera Gaertn.)

The Indian lotus (Nelumbo nucifera Gaertn.) is a popular ornamental plant and a source of traditional herbal medicine. Its various parts are widely used in the pharmaceutical, cosmeceutical, and nutraceutical industries. The recent study aimed to develop a high-frequency in vitro regeneration system in Indian lotus. This study utilizes the pink lotus cultivar from the Botanical Garden of CSIR-NBRI Lucknow. The study was successfully achieved through direct and indirect methods using different plant growth regulators (PGRs). A direct regeneration system was established using explants shoot apical meristem and plumule cultured on SIM supplemented with 17 combinations and concentrations of BAP and NAA. Both explants produced the highest number of shoots with a combination of 4.44 μM BAP and 0.55 μM NAA. The highest number of shoots per explant 25 ± 1.0 was developed from the shoot apical meristem, while the plumule explant developed 16.3 ± 0.5 shoots per explant. Thereafter, the plantlets were transferred to LRIM, which contained 17 combinations and concentrations of NAA or IBA and BAP. The maximum number of roots, per explant 23.6 ± 0.5, was developed from shoot apical meristem using 2.22 μM NAA and 0.54 μM BAP. The highest number of roots, per explant 23 ± 1.0, was developed from the plumule using 4.44 μM IBA. Indirect somatic embryogenesis has been established through callus culture. The leaf segments were cultured onto a callus induction medium supplemented with ten combinations of 2,4-D and BAP. The high-frequency callus formation 24.33 ± 0.5 was obtained with a 5.0 μM 2,4-D and 1.0 μM BAP combination. All developmental stages at the proembryo, globular, heart, torpedo, and mature embryos were formed on concentrations of 2,4-D and BAP. After inducing shoot and root growth, well-developed plantlets were transferred to the greenhouse, resulting in a success rate of 18.47%.

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来源期刊
CiteScore
5.00
自引率
7.70%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Founded in 1965, In Vitro Cellular & Developmental Biology - Plant is the only journal devoted solely to worldwide coverage of in vitro biology in plants. Its high-caliber original research and reviews make it required reading for anyone who needs comprehensive coverage of the latest developments and state-of-the-art research in plant cell and tissue culture and biotechnology from around the world.
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