{"title":"体外繁殖三倍体 Toxicodendron vernicifluum (Stokes) F. A. Barkl","authors":"Xiaolin Zhang, Jiayu Feng, Yiran Luo, Xiaomeng Duan, Chengzhong He, Dan Zong","doi":"10.1007/s11627-024-10452-x","DOIUrl":null,"url":null,"abstract":"<p>Triploid lacquer trees have a higher yield of raw lacquer and greater stress resistance; however, it is challenging to develop efficient and rapid seedling culture techniques because of the influence of metabolites with raw lacquer. In this study, the stem segments of a triploid lacquer tree were used to investigate the effects of explant sterilization treatment, different combinations of exogenous hormone concentrations on the induction of axillary buds, seedling development, and rooting, as well as transplanting substrate ratios. The results showed that the optimal combination of disinfection treatment for triploid <i>Toxicodendron vernicifluum</i> (Stokes) F. A. Barkl explants involves 75% alcohol immersion for 10 s, followed by 2.0% benzalkonium chloride treatment for 1 min and 0.1% HgCl<sub>2</sub> treatment for 4 min. The suitable medium for axillary bud induction was Murashige and Skoog (MS) containing 0.1 mg•L<sup>−1</sup> zeatin (ZT) and 0.1 mg•L<sup>−1</sup> 6-benzylaminopurine (6-BA), resulting in a high induction rate of 95.0%. Use of 1/2 MS supplemented with 0.5 mg•L<sup>−1</sup> 6-BA and 0.3 mg•L<sup>−1</sup> 2,4-dichlorophenoxyacetic acid (2,4-D) was the most effective for axillary bud proliferation. The combination of 1/2 MS containing 0.1 mg•L<sup>−1</sup> 6-BA, 0.5 mg•L<sup>−1</sup> 2,4-D, 0.5 mg•L<sup>−1</sup> indole-3-butyric acid (IBA), and 0.2% activated carbon (AC) was an optimum for hardening-off. With woody plant medium (WPM) supplemented with 0.5 mg•L<sup>−1</sup> naphthaleneacetic acid (NAA) and 0.5 mg•L<sup>−1</sup> 2,4-D and 0.5 g•L<sup>−1</sup> AC as a rooting medium, the rooting rate was 67.8%. Also, the appropriate transplanting substrate ratio for triploid <i>Toxicodendron vernicifluum</i> (Stokes) F. A. Barkl was grass charcoal:laterite:humus = 1:1:1. Collectively, the results provided the technical assurance for seedling scale multiplication and propagation, facilitating its application in production.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"273 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In vitro propagation of triploid Toxicodendron vernicifluum (Stokes) F. A. 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Barkl explants involves 75% alcohol immersion for 10 s, followed by 2.0% benzalkonium chloride treatment for 1 min and 0.1% HgCl<sub>2</sub> treatment for 4 min. The suitable medium for axillary bud induction was Murashige and Skoog (MS) containing 0.1 mg•L<sup>−1</sup> zeatin (ZT) and 0.1 mg•L<sup>−1</sup> 6-benzylaminopurine (6-BA), resulting in a high induction rate of 95.0%. Use of 1/2 MS supplemented with 0.5 mg•L<sup>−1</sup> 6-BA and 0.3 mg•L<sup>−1</sup> 2,4-dichlorophenoxyacetic acid (2,4-D) was the most effective for axillary bud proliferation. The combination of 1/2 MS containing 0.1 mg•L<sup>−1</sup> 6-BA, 0.5 mg•L<sup>−1</sup> 2,4-D, 0.5 mg•L<sup>−1</sup> indole-3-butyric acid (IBA), and 0.2% activated carbon (AC) was an optimum for hardening-off. With woody plant medium (WPM) supplemented with 0.5 mg•L<sup>−1</sup> naphthaleneacetic acid (NAA) and 0.5 mg•L<sup>−1</sup> 2,4-D and 0.5 g•L<sup>−1</sup> AC as a rooting medium, the rooting rate was 67.8%. Also, the appropriate transplanting substrate ratio for triploid <i>Toxicodendron vernicifluum</i> (Stokes) F. A. Barkl was grass charcoal:laterite:humus = 1:1:1. 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引用次数: 0
摘要
三倍体漆树具有更高的生漆产量和更强的抗逆性;然而,由于生漆代谢产物的影响,开发高效快速的幼苗培养技术具有挑战性。本研究利用三倍体漆树的茎段,研究了外植体消毒处理、不同浓度的外源激素组合对腋芽诱导、幼苗发育和生根的影响,以及移栽基质配比。结果表明,三倍体 Toxicodendron vernicifluum (Stokes) F. A. Barkl 外植体消毒处理的最佳组合是 75% 酒精浸泡 10 秒,然后 2.0% 苯扎氯铵处理 1 分钟和 0.1% HgCl2 处理 4 分钟。腋芽诱导的合适培养基是含有 0.1 mg-L-1 玉米素(ZT)和 0.1 mg-L-1 6-苄基氨基嘌呤(6-BA)的 Murashige and Skoog(MS),其诱导率高达 95.0%。添加 0.5 mg-L-1 6-BA 和 0.3 mg-L-1 2,4-二氯苯氧乙酸(2,4-D)的 1/2 MS 对腋芽增殖最有效。含有 0.1 mg-L-1 6-BA、0.5 mg-L-1 2,4-D、0.5 mg-L-1 吲哚-3-丁酸(IBA)和 0.2% 活性碳(AC)的 1/2 MS 组合是硬化的最佳选择。用木质植物培养基(WPM)添加 0.5 mg-L-1 萘乙酸(NAA)、0.5 mg-L-1 2,4-D 和 0.5 g-L-1 AC 作为生根培养基,生根率为 67.8%。此外,三倍体 Toxicodendron vernicifluum (Stokes) F. A. Barkl 的适宜移植基质比例为草炭:红土:腐殖土=1:1:1。总之,这些结果为幼苗规模化繁殖提供了技术保证,有利于其在生产中的应用。
In vitro propagation of triploid Toxicodendron vernicifluum (Stokes) F. A. Barkl
Triploid lacquer trees have a higher yield of raw lacquer and greater stress resistance; however, it is challenging to develop efficient and rapid seedling culture techniques because of the influence of metabolites with raw lacquer. In this study, the stem segments of a triploid lacquer tree were used to investigate the effects of explant sterilization treatment, different combinations of exogenous hormone concentrations on the induction of axillary buds, seedling development, and rooting, as well as transplanting substrate ratios. The results showed that the optimal combination of disinfection treatment for triploid Toxicodendron vernicifluum (Stokes) F. A. Barkl explants involves 75% alcohol immersion for 10 s, followed by 2.0% benzalkonium chloride treatment for 1 min and 0.1% HgCl2 treatment for 4 min. The suitable medium for axillary bud induction was Murashige and Skoog (MS) containing 0.1 mg•L−1 zeatin (ZT) and 0.1 mg•L−1 6-benzylaminopurine (6-BA), resulting in a high induction rate of 95.0%. Use of 1/2 MS supplemented with 0.5 mg•L−1 6-BA and 0.3 mg•L−1 2,4-dichlorophenoxyacetic acid (2,4-D) was the most effective for axillary bud proliferation. The combination of 1/2 MS containing 0.1 mg•L−1 6-BA, 0.5 mg•L−1 2,4-D, 0.5 mg•L−1 indole-3-butyric acid (IBA), and 0.2% activated carbon (AC) was an optimum for hardening-off. With woody plant medium (WPM) supplemented with 0.5 mg•L−1 naphthaleneacetic acid (NAA) and 0.5 mg•L−1 2,4-D and 0.5 g•L−1 AC as a rooting medium, the rooting rate was 67.8%. Also, the appropriate transplanting substrate ratio for triploid Toxicodendron vernicifluum (Stokes) F. A. Barkl was grass charcoal:laterite:humus = 1:1:1. Collectively, the results provided the technical assurance for seedling scale multiplication and propagation, facilitating its application in production.
期刊介绍:
Founded in 1965, In Vitro Cellular & Developmental Biology - Plant is the only journal devoted solely to worldwide coverage of in vitro biology in plants. Its high-caliber original research and reviews make it required reading for anyone who needs comprehensive coverage of the latest developments and state-of-the-art research in plant cell and tissue culture and biotechnology from around the world.