In vitro regeneration protocol for endemic Campanula leblebicii Yıldırım

IF 2.2 3区 生物学 Q4 CELL BIOLOGY
Yasemin Kartal, Başar Sevindik, Ademi Fahri Pirhan
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Abstract

Campanula leblebicii, discovered by Yıldırım, is found only in the village of Ovacık in the Kemalpaşa district of İzmir province, and it is one of the valuable endemic species for Turkey flora. Petiole and leaf explants were cultured on full-strength Murashige and Skoog (MS) medium containing 30.0 g L−1 sucrose; 4.0 g L−1 Gelrite; 0.3 mg L−1 1-naphthaleneacetic acid (NAA); 0, 0.5, 1.0, 2.0, or 3.0 mg L−1 zeatin (ZEA); and 0, 0.5, 1.0, 2.0, or 3.0 mg L−1 6-benzyladenine (BA) for callus initiation. To obtain shoot formation, callus was transferred to full-strength MS medium containing 0.3 mg L−1 NAA; 0, 0.5, 1.0, 2.0, or 3.0 mg L−1 gibberellic acid (GA3); and 0, 0.5, 1.0, 2.0, 3.0 mg L−1 BA. Efficient callus (93.33%) and shoot formation (73.33) were observed from petiole explants cultured on MS medium containing 0.3 mg L−1 NAA and 1.0 mg L−1 BA. Rooting was obtained spontaneously in all shoot initiation medium, but efficient rooting occurred on MS medium supplemented with 0.3 mg L−1 NAA and 1.0 mg L−1 BA. Plants were acclimatized to the pots including sterile peat and perlite (1:1, v/v), and, in total, 12 plants were acclimatized from one genotype.

Abstract Image

地方性金钟罩(Campanula leblebicii Yıldırım )的体外再生方案
Yıldırım 发现的 Campanula leblebicii 仅见于伊兹密尔省 Kemalpaşa 地区的 Ovacık 村,是土耳其植物区系中珍贵的特有物种之一。叶柄和叶片外植体在全强度的 Murashige 和 Skoog(MS)培养基上培养,培养基中含有 30.0 g L-1 蔗糖;4.0 g L-1 Gelrite;0.3 mg L-1 1-萘乙酸(NAA);0、0.5、1.0、2.0 或 3.0 mg L-1玉米素(ZEA);以及 0、0.5、1.0、2.0 或 3.0 mg L-1 6-苄基腺嘌呤(BA)。为了获得芽的形成,将胼胝体转移到含有 0.3 mg L-1 NAA;0、0.5、1.0、2.0 或 3.0 mg L-1 赤霉素(GA3);以及 0、0.5、1.0、2.0、3.0 mg L-1 BA 的全强度 MS 培养基中。在含有 0.3 mg L-1 NAA 和 1.0 mg L-1 BA 的 MS 培养基上培养的叶柄外植体可观察到高效的胼胝体(93.33%)和芽形成(73.33%)。在所有芽萌发培养基上都能自然生根,但在添加 0.3 mg L-1 NAA 和 1.0 mg L-1 BA 的 MS 培养基上生根效率较高。植株在包括无菌泥炭和珍珠岩(1:1,v/v)的花盆中进行了适应性栽培,一个基因型共适应性栽培了 12 株植株。
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来源期刊
CiteScore
5.00
自引率
7.70%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Founded in 1965, In Vitro Cellular & Developmental Biology - Plant is the only journal devoted solely to worldwide coverage of in vitro biology in plants. Its high-caliber original research and reviews make it required reading for anyone who needs comprehensive coverage of the latest developments and state-of-the-art research in plant cell and tissue culture and biotechnology from around the world.
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