Yuping Xiong, Zhenpeng Wei, Junyu Liu, Jianrong Li, Shuguang Jian, Xinhua Zhang, Yuan Li, Zhan Bian, Kunlin Wu, Songjun Zeng, Jaime A. Teixeira da Silva, Guohua Ma
{"title":"Axonopus compressus (Swartz) Beauv 的嫩枝繁殖、再生、胼胝体诱导和分化","authors":"Yuping Xiong, Zhenpeng Wei, Junyu Liu, Jianrong Li, Shuguang Jian, Xinhua Zhang, Yuan Li, Zhan Bian, Kunlin Wu, Songjun Zeng, Jaime A. Teixeira da Silva, Guohua Ma","doi":"10.1007/s11627-024-10432-1","DOIUrl":null,"url":null,"abstract":"<p><i>Axonopus compressus</i> (Swartz) Beauv., a perennial herb in the Poaceae that has been introduced to a number of tropical and subtropical countries and regions, can serve as a lawn ground cover while its leaves can be developed as a bioenergy resource. It also displays some resistance to heavy metals, allowing it to be planted in areas and urban green spaces polluted by heavy metals. <i>A. compressus</i> is also used as a traditional Chinese medicine. This study is the first report on tissue culture of <i>A. compressus</i>. Stem explants induced shoot clusters on 6-benzyladenine (BA)-containing Murashige and Skoog (MS) medium, with 2.0 mg L<sup>–1</sup> BA inducing a shoot proliferation coefficient (SPC) of 10.89 within 30 d, while MS medium containing 2.0 mg L<sup>–1</sup> BA and 0.1 mg L<sup>–1</sup> α-naphthaleneacetic acid (NAA) amplified SPC to 12.88 within 30 d. SPC on MS medium supplemented with kinetin (KIN) or thidiazuron (TDZ) was not as high as on medium with BA and never exceeded 3.57 within 30 d. <i>A. compressus</i> formed adventitious roots easily, within 15 d, most efficiently on ½MS medium supplemented with 0.1 to 0.5 mg L<sup>–1</sup> IAA, NAA, or IBA, or even on auxin-free medium. Resulting plantlets displayed a high survival rate (> 98%) when transplanted to a substrate containing humus, or humus mixed with vermiculite or sand. Callus was also successfully induced from leaf sheaths on MS medium containing 1.0 mg L<sup>–1</sup> 2,4-dichlorophenoxyacetic acid, even more so when 1.15 g L<sup>–1</sup> <span>l</span>-proline was added. Induced callus was able to differentiate into adventitious shoots on MS medium with 0.1 to 0.5 mg L<sup>–1</sup> KIN or BA. An efficient system of shoot proliferation, callus induction, and regeneration in <i>A. compressus</i> has been established.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"27 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Shoot propagation, regeneration, and callus induction and differentiation, of Axonopus compressus (Swartz) Beauv\",\"authors\":\"Yuping Xiong, Zhenpeng Wei, Junyu Liu, Jianrong Li, Shuguang Jian, Xinhua Zhang, Yuan Li, Zhan Bian, Kunlin Wu, Songjun Zeng, Jaime A. Teixeira da Silva, Guohua Ma\",\"doi\":\"10.1007/s11627-024-10432-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><i>Axonopus compressus</i> (Swartz) Beauv., a perennial herb in the Poaceae that has been introduced to a number of tropical and subtropical countries and regions, can serve as a lawn ground cover while its leaves can be developed as a bioenergy resource. It also displays some resistance to heavy metals, allowing it to be planted in areas and urban green spaces polluted by heavy metals. <i>A. compressus</i> is also used as a traditional Chinese medicine. This study is the first report on tissue culture of <i>A. compressus</i>. Stem explants induced shoot clusters on 6-benzyladenine (BA)-containing Murashige and Skoog (MS) medium, with 2.0 mg L<sup>–1</sup> BA inducing a shoot proliferation coefficient (SPC) of 10.89 within 30 d, while MS medium containing 2.0 mg L<sup>–1</sup> BA and 0.1 mg L<sup>–1</sup> α-naphthaleneacetic acid (NAA) amplified SPC to 12.88 within 30 d. SPC on MS medium supplemented with kinetin (KIN) or thidiazuron (TDZ) was not as high as on medium with BA and never exceeded 3.57 within 30 d. <i>A. compressus</i> formed adventitious roots easily, within 15 d, most efficiently on ½MS medium supplemented with 0.1 to 0.5 mg L<sup>–1</sup> IAA, NAA, or IBA, or even on auxin-free medium. Resulting plantlets displayed a high survival rate (> 98%) when transplanted to a substrate containing humus, or humus mixed with vermiculite or sand. Callus was also successfully induced from leaf sheaths on MS medium containing 1.0 mg L<sup>–1</sup> 2,4-dichlorophenoxyacetic acid, even more so when 1.15 g L<sup>–1</sup> <span>l</span>-proline was added. Induced callus was able to differentiate into adventitious shoots on MS medium with 0.1 to 0.5 mg L<sup>–1</sup> KIN or BA. An efficient system of shoot proliferation, callus induction, and regeneration in <i>A. compressus</i> has been established.</p>\",\"PeriodicalId\":13293,\"journal\":{\"name\":\"In Vitro Cellular & Developmental Biology - Plant\",\"volume\":\"27 1\",\"pages\":\"\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"In Vitro Cellular & Developmental Biology - Plant\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s11627-024-10432-1\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"In Vitro Cellular & Developmental Biology - Plant","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11627-024-10432-1","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
Axonopus compressus (Swartz) Beauv.是一种多年生草本植物,属于蒲葵科,已被引入多个热带和亚热带国家和地区。它还对重金属有一定的抗性,因此可以种植在受重金属污染的地区和城市绿地。压片草还被用作传统中药。本研究首次报道了压片草的组织培养。茎外植体在含 6-苄基腺嘌呤(BA)的 Murashige and Skoog(MS)培养基上诱导出芽簇,2.0 mg L-1 BA 在 30 d 内诱导出 10.89 的芽增殖系数(SPC),而含 2.0 mg L-1 BA 和 0.1 mg L-1 α-萘乙酸(NAA)的 MS 培养基在 30 d 内将 SPC 放大到 12.88。在添加了 0.1 至 0.5 mg L-1 IAA、NAA 或 IBA 的 ½MS 培养基上,甚至在不含助剂的培养基上,压条菌都能在 15 d 内最有效地形成不定根。当移栽到含有腐殖质或腐殖质与蛭石或沙子混合的基质上时,结果植株显示出很高的存活率(98%)。在含有 1.0 mg L-1 2,4-dichlorophenoxyacetic acid 的 MS 培养基上,也能成功地从叶鞘中诱导出胼胝体。在含有 0.1 至 0.5 mg L-1 KIN 或 BA 的 MS 培养基上,诱导的胼胝体能够分化为不定芽。已经建立了一套有效的压条莲芽增殖、胼胝体诱导和再生系统。
Shoot propagation, regeneration, and callus induction and differentiation, of Axonopus compressus (Swartz) Beauv
Axonopus compressus (Swartz) Beauv., a perennial herb in the Poaceae that has been introduced to a number of tropical and subtropical countries and regions, can serve as a lawn ground cover while its leaves can be developed as a bioenergy resource. It also displays some resistance to heavy metals, allowing it to be planted in areas and urban green spaces polluted by heavy metals. A. compressus is also used as a traditional Chinese medicine. This study is the first report on tissue culture of A. compressus. Stem explants induced shoot clusters on 6-benzyladenine (BA)-containing Murashige and Skoog (MS) medium, with 2.0 mg L–1 BA inducing a shoot proliferation coefficient (SPC) of 10.89 within 30 d, while MS medium containing 2.0 mg L–1 BA and 0.1 mg L–1 α-naphthaleneacetic acid (NAA) amplified SPC to 12.88 within 30 d. SPC on MS medium supplemented with kinetin (KIN) or thidiazuron (TDZ) was not as high as on medium with BA and never exceeded 3.57 within 30 d. A. compressus formed adventitious roots easily, within 15 d, most efficiently on ½MS medium supplemented with 0.1 to 0.5 mg L–1 IAA, NAA, or IBA, or even on auxin-free medium. Resulting plantlets displayed a high survival rate (> 98%) when transplanted to a substrate containing humus, or humus mixed with vermiculite or sand. Callus was also successfully induced from leaf sheaths on MS medium containing 1.0 mg L–1 2,4-dichlorophenoxyacetic acid, even more so when 1.15 g L–1l-proline was added. Induced callus was able to differentiate into adventitious shoots on MS medium with 0.1 to 0.5 mg L–1 KIN or BA. An efficient system of shoot proliferation, callus induction, and regeneration in A. compressus has been established.
期刊介绍:
Founded in 1965, In Vitro Cellular & Developmental Biology - Plant is the only journal devoted solely to worldwide coverage of in vitro biology in plants. Its high-caliber original research and reviews make it required reading for anyone who needs comprehensive coverage of the latest developments and state-of-the-art research in plant cell and tissue culture and biotechnology from around the world.