Mannitol and sorbitol concentration optimization for effective Epipactis flava Seidenf. in vitro slow growth storage

IF 2.2 3区 生物学 Q4 CELL BIOLOGY
Julaluk Linjikao, Phithak Inthima, Apinun Limmongkon, Anupan Kongbangkerd
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Abstract

Epipactis flava Seidenf., a Thai rheophytic orchid, is endangered due to habitat destruction and climate change. In vitro conservation serves as an effective technique for the ex situ preservation of orchid diversity. This study investigated the in vitro storage of E. flava seedlings under slow growth conditions using Murashige and Skoog (MS) medium supplemented with either sorbitol or mannitol individually at concentrations of 1.0%, 2.0%, 3.0%, and 4.0% (w/v) for 24 wk without subculture. The storage medium added with 3.0% (w/v) sucrose was used as the control. After 24 wk of storage, the storage medium containing 2.0% mannitol provided the highest survival rate (97.6%) while the highest leaf formation (70.8%) was achieved with 3.0% sucrose. The addition of 1.0 to 2.0% sorbitol, 1.0% mannitol, and 3.0 to 4.0% mannitol showed the highest shoot bud formation (100%). At the end of 24 wk, the plantlets were transferred to fresh medium for an 8-wk growth recovery. The plantlets derived from the storage medium with 3.0% mannitol exhibited the highest survival rate (73.5%). Conversely, the highest number of shoot buds (9.9 shoot buds per plantlet) was found on plantlets derived from storage medium with 1.0% sorbitol while the highest number of shoots (4.8 shoots per plantlet) was achieved in plantlets derived from 1.0% mannitol. Consequently, these results suggested that the storage medium with 3.0% mannitol is optimal for conserving plantlets for up to 24 wk, and all plantlets could successfully produce shoot buds and shoots during in vitro recovery. Hence, this strategy has potential in the conservation and utilization of this endangered species.

Abstract Image

优化甘露醇和山梨醇的浓度,以实现 Epipactis flava Seidenf.
Epipactis flava Seidenf.是一种泰国流苏兰,由于栖息地遭到破坏和气候变化而濒临灭绝。离体保存是异地保护兰花多样性的有效技术。本研究使用添加了山梨醇或甘露醇(浓度分别为 1.0%、2.0%、3.0% 和 4.0%(w/v))的 Murashige 和 Skoog(MS)培养基,在生长缓慢的条件下对花叶酢浆草幼苗进行了 24 周的体外贮藏,且无需进行亚培养。添加 3.0%(重量/体积)蔗糖的贮藏培养基作为对照。贮藏 24 周后,含 2.0% 甘露醇的贮藏培养基的存活率最高(97.6%),而含 3.0% 蔗糖的贮藏培养基的成叶率最高(70.8%)。添加 1.0% 至 2.0% 山梨醇、1.0% 甘露醇和 3.0% 至 4.0% 甘露醇时,芽的形成率最高(100%)。在 24 周结束时,将小植株转移到新鲜培养基中,进行为期 8 周的生长恢复。从含 3.0% 甘露醇的贮藏培养基中提取的小植株成活率最高(73.5%)。相反,从含 1.0% 山梨糖醇的贮藏培养基中提取的小植株的芽数量最多(每个小植株有 9.9 个芽苞),而从含 1.0% 甘露醇的贮藏培养基中提取的小植株的芽数量最多(每个小植株有 4.8 个芽苞)。因此,这些结果表明,含 3.0% 甘露醇的贮藏培养基是保存小植株长达 24 周的最佳培养基,而且所有小植株都能在离体恢复期间成功产生芽和嫩枝。因此,这一策略在保护和利用这一濒危物种方面具有潜力。
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来源期刊
CiteScore
5.00
自引率
7.70%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Founded in 1965, In Vitro Cellular & Developmental Biology - Plant is the only journal devoted solely to worldwide coverage of in vitro biology in plants. Its high-caliber original research and reviews make it required reading for anyone who needs comprehensive coverage of the latest developments and state-of-the-art research in plant cell and tissue culture and biotechnology from around the world.
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