Silver nitrate mediated improvement in micropropagation and amelioration of micro-morpho-anatomical structures in Oxystelma esculentum (L.f.) Sm.

IF 2.2 3区生物学 Q4 CELL BIOLOGY

In Vitro Cellular & Developmental Biology - Plant Pub Date : 2024-03-05 DOI:10.1007/s11627-024-10417-0

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引用次数: 0

Abstract

The present study highlights the effect of silver nitrate (AgNO₃) in the promotion of in vitro regeneration and morpho-structural growth and developments in Oxystelma esculentum (L.f.) Sm. The incorporation of AgNO₃ into the Murashige and Skoog (MS) medium resulted in a favorable enhancement of shoot proliferation. Among the different concentrations of AgNO₃ screened, 0.5 mg L⁻¹ AgNO₃ along with 0.5 mg L⁻¹ meta-Topolin (mT) and 0.25 mg L⁻¹ indole-3-acetic acid (IAA) stimulated the highest count, and length of shoots (143 shoots and 14.0 cm length) after 3 mo of incubation and the 3rd subculture. The in vitro–induced deformities in the tissues of the control stem and foliages were improved when the shoots were grown in AgNO₃-enriched medium. Silver nitrate increased the photosynthetic pigments and improved the formation of functional stomata. The AgNO₃-supplemented medium-derived shoots exhibited accelerated adventitious root induction (100% rhizogenesis, 21.0 roots per shoot) when the cut ends of shoots were treated with 200.0 mg L⁻¹ indole-3-butyric acid (IBA) for 5 min under greenhouse conditions. The incorporation of AgNO₃ with optimal plant growth regulators in the medium promoted overall growth. It prevented in vitro–induced morphological and anatomical anomalies in the leaf and stem, which considerably improved the greenhouse and in vivo survival of micropropagated plantlets.

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硝酸银介导的 Oxystelma esculentum（L.f. Sm.

摘要本研究强调了硝酸银（AgNO3）对促进Oxystelma esculentum（L.f.）Sm.离体再生和形态结构生长发育的影响。在 Murashige 和 Skoog（MS）培养基中加入 AgNO3 能有效促进芽的增殖。在筛选出的不同浓度的 AgNO3 中，0.5 mg L-1 AgNO3 与 0.5 mg L-1 meta-Topolin (mT) 和 0.25 mg L-1 indole-3-acetic acid (IAA) 在培养 3 个月和第 3 次亚培养后刺激的芽数量和长度（143 个芽和 14.0 cm 长）最高。在富含 AgNO3 的培养基中培育嫩枝时，离体诱导的对照茎和叶组织畸形得到了改善。硝酸银增加了光合色素，改善了功能气孔的形成。在温室条件下，用 200.0 mg L-1 的吲哚-3-丁酸（IBA）处理嫩枝切端 5 分钟，AgNO3 补充培养基衍生的嫩枝表现出加速的不定根诱导（100% 根瘤发生，每枝 21.0 根）。在培养基中加入 AgNO3 和最佳植物生长调节剂可促进整体生长。它防止了体外诱导的叶片和茎的形态和解剖异常，从而大大提高了微繁殖植株在温室和体内的存活率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

In Vitro Cellular & Developmental Biology - Plant 生物-发育生物学

CiteScore

5.00

自引率

7.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Founded in 1965, In Vitro Cellular & Developmental Biology - Plant is the only journal devoted solely to worldwide coverage of in vitro biology in plants. Its high-caliber original research and reviews make it required reading for anyone who needs comprehensive coverage of the latest developments and state-of-the-art research in plant cell and tissue culture and biotechnology from around the world.