Low-temperature storage in dark condition improved the in vitro regeneration of Plumbago zeylanica synthetic seeds: a medicinally valuable species

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Ravishankar Chauhan, Priya Banjare, Subir Kumar Parey, Afreen Anjum, Afaque Quraishi
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Abstract

Medicinal applications of Plumbago zeylanica and its metabolites on various diseases and low viability and inconsistent germination of its seeds are the reasons behind the loss of its genetic diversity. Hence, an efficient protocol for the short-term storage of P. zeylanica synthetic seeds, which is an overexploited medicinally valuable plant, was developed. Initially, in vitro culture was performed from nodal explants to develop synthetic seeds from its proliferated shoots. Murashige and Skoog (MS) medium augmented with 0.5 mg L−1 6-benzylaminopurine (BAP) resulted in the best morphogenetic response. Thereafter, the developed synseeds were stored for 2 wk at a temperature of 10 or 25°C in different conditions and further evaluated for regeneration. Higher re-growth rate (80%) and the identical morphogenetic response were recorded for the P. zeylanica synthetic seeds, which were stored at a temperature of 10°C in dark condition after its storage period. As per the available literature, this is the first report pertaining to in vitro low-temperature storage of synthetic seeds of P. zeylanica and can further be utilized for the conservation of elite clones for the study of medicinally potent species.

Abstract Image

黑暗条件下的低温贮藏改善了药用珍贵树种板蓝根(Plumbago zeylanica)合成种子的体外再生能力
Plumbago zeylanica 及其代谢物可用于治疗各种疾病,但其种子存活率低且发芽率不稳定,这是其遗传多样性丧失的原因。因此,我们开发了一种有效的方法,用于短期贮藏被过度开发的药用植物 P. zeylanica 合成种子。最初,从节的外植体进行离体培养,从其增殖的嫩枝中培育合成种子。在 Murashige 和 Skoog(MS)培养基中添加 0.5 mg L-1 6-苄基氨基嘌呤(BAP)可获得最佳的形态发生反应。之后,将发育好的合子在 10 或 25°C 的不同温度条件下贮藏 2 周,并进一步评估其再生情况。泽兰合成种子的再生率更高(80%),形态发生反应也相同。根据现有文献,这是第一份关于离体低温贮藏泽兰合成种子的报告,可进一步用于保存精英克隆,以研究具有药效的物种。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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