{"title":"In vitro micropropagation of a novel non-flowering radiation mutant of Platanus × acerifolia Willd.","authors":"","doi":"10.1007/s11627-023-10409-6","DOIUrl":null,"url":null,"abstract":"<h3>Abstract</h3> <p>An efficient <em>in vitro</em> micropropagation system for <em>Platanus</em> × <em>acerifolia</em> ‘F03’, a radiation mutant that has not flowered for 19 yr, has been established. Axillary buds were used as explants. After sprouting on Murashige and Skoog medium (MS) supplemented with 0.3 mg·L<sup>−1</sup> 6-benzylaminopurine (6-BA) together with 0.05 mg·L<sup>−1</sup> α-naphthaleneacetic acid (NAA), the best medium for multiplication and elongation was MS basal medium supplemented with 0.1 mg·L<sup>−1</sup> 6-benzylaminopurine and with a multiplication rate of 3.43 ± 0.57. Plantlets over 1.5 cm could root on McCown Woody Plant Medium (WPM) supplemented with 0.01 mg·L<sup>−1</sup> α-naphthaleneacetic acid and 7.0 g·L<sup>−1</sup> agar with an ideal rooting rate and transplanting survival rate of 100 ± 0%. The optimum survival rate was possible when trays were covered with a thin plastic lid for more than 14 d. After transplanting, air humidity and temperature were crucial for young plant survival. The system established here could serve as a pathway for commercial production of the line and provide sterile leaf explants for regeneration and further genetic improvement of the species.</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"1 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"In Vitro Cellular & Developmental Biology - Plant","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11627-023-10409-6","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
An efficient in vitro micropropagation system for Platanus × acerifolia ‘F03’, a radiation mutant that has not flowered for 19 yr, has been established. Axillary buds were used as explants. After sprouting on Murashige and Skoog medium (MS) supplemented with 0.3 mg·L−1 6-benzylaminopurine (6-BA) together with 0.05 mg·L−1 α-naphthaleneacetic acid (NAA), the best medium for multiplication and elongation was MS basal medium supplemented with 0.1 mg·L−1 6-benzylaminopurine and with a multiplication rate of 3.43 ± 0.57. Plantlets over 1.5 cm could root on McCown Woody Plant Medium (WPM) supplemented with 0.01 mg·L−1 α-naphthaleneacetic acid and 7.0 g·L−1 agar with an ideal rooting rate and transplanting survival rate of 100 ± 0%. The optimum survival rate was possible when trays were covered with a thin plastic lid for more than 14 d. After transplanting, air humidity and temperature were crucial for young plant survival. The system established here could serve as a pathway for commercial production of the line and provide sterile leaf explants for regeneration and further genetic improvement of the species.
期刊介绍:
Founded in 1965, In Vitro Cellular & Developmental Biology - Plant is the only journal devoted solely to worldwide coverage of in vitro biology in plants. Its high-caliber original research and reviews make it required reading for anyone who needs comprehensive coverage of the latest developments and state-of-the-art research in plant cell and tissue culture and biotechnology from around the world.