Horticulture Environment and Biotechnology最新文献

{"title":"Identification and expression analysis of the bZIP and WRKY gene families during anthocyanins biosynthesis in Lagerstroemia indica L","authors":"Cuihua Gu, Sidan Hong, Jie Wang, Linxue Shang, Guozhe Zhang, Yu Zhao, Qingqing Ma, Dandan Ma","doi":"10.1007/s13580-023-00551-w","DOIUrl":"https://doi.org/10.1007/s13580-023-00551-w","url":null,"abstract":"<p><i>Lagerstroemia indica</i> is a summer ornamental flowering tree, belonging to the Lythraceae family. Its flower color is a key ornamental characteristic. Recent research indicates that WRKY and bZIP transcription factors play important role in flower color formation. Based on the transcriptome database of <i>L. indica</i>, this study identified 85 <i>LibZIPs</i> and 61 <i>LiWRKYs</i> from the <i>L. indica</i> transcriptome database and analyzed their expression profiles using qRT-PCR. Phylogenetic analysis divided <i>LibZIPs</i> into 11 subfamilies and <i>LiWRKYs</i> into six subfamilies. Nine <i>LibZIPs</i> and four <i>LiWRKYs</i> were found to have higher expression in colored samples compared to the white sample. <i>LibZIP31</i>, <i>LibZIP65</i>, <i>LibZIP34</i>, <i>LiWRKY16</i>, <i>LiWRKY25</i>, <i>LiWRKY40</i>, and <i>LiWRKY67</i> may play important roles in flower color formation in <i>L. indica</i>. These results provide valuable information for further functional analysis of the bZIP and WRKY genes families in <i>L. indica</i> and help clarify their roles in flower color formation.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139475466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, culture of Platycodon grandiflorus protoplasts: factors affecting protoplast yield, cell division, and micro-callus formation","authors":"Suk-Hyun Kwon, Hosakatte Niranjana Murthy, Jong-Eun Han, Hon-Sol Lee, So-Young Park","doi":"10.1007/s13580-023-00585-0","DOIUrl":"https://doi.org/10.1007/s13580-023-00585-0","url":null,"abstract":"<p><i>Plyatocodon grandiflorus</i> is an important medicinal herb, its rhizomes are utilized as vegetables and as medicinal food. In this investigation, we were able to successfully isolate protoplasts from <i>P. grandiflorus</i> leaf mesophyll tissues in order to promote the development of calli. It has been determined what are the ideal conditions for protoplast isolation, involving the combination of enzymes, the osmoticum, and the length of enzyme digestion. For the most effective results, cell protoplast washing (CPW) medium with 1.0% Viscozyme® L + 3% Celluclast® 1.5 L + 0.5% Pectinex® XXL + 0.4 M mannitol and 8 h of incubation was used to isolate protoplasts from leaf mesophyll tissues. The thin alginate layer (TAL) approach was utilized to embed the protoplasts. Subsequently, TAL segments were cultured in Murashige and Skoog (MS) media supplemented with 3% sucrose, 2,4-dichlorophenoxyacetic acid (2,4-D, 0, 0.56, 1.12 µM), and phytosulfokine (PSK, 0, 0.05, 0.1 µM). The optimal concentration of 2,4-D and PSK for initiation of protoplast division, multicell development, and micro-callus formation was predicted using response surface methodology (RSM) with central composite design (CCD). Based on the CCD responses, it was determined that 1.12 µM 2,4-D and 0.065 µM PSK were the best concentrations to induce protoplast division and the formation of micro-calluses. The acquired results are valuable for <i>P. grandiflorus</i> protoplast isolation and culture; however, coordinated efforts are required for plant regeneration using callus produced from protoplasts.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139476918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of inoculating different mycorrhizal fungi on rhizosphere soil fungi and nutrient uptake of blueberry","authors":"Zejun Song, Longhai Xiao, Xiaolan Guo, Yunzheng Zhu, Xiaoli An, Yi Tan, Xinyu Zhang, Delu Wang","doi":"10.1007/s13580-023-00527-w","DOIUrl":"https://doi.org/10.1007/s13580-023-00527-w","url":null,"abstract":"<p>The aim of this study was to explore the effects of inoculating with different fungi on the structural characteristics of soil fungal communities and the nutrient absorption of blueberry plants. The fungi DSE (<i>Phialocephala fortinii</i>), SS (<i>Penicillium pinophilum</i>), ZB (<i>Cladosporium cladosporioides</i>), QMK (<i>Chaetomium globosum</i>), and LZ (<i>Schizophyllum commune</i>) were selected for this study. The soil fungal diversity and community structure in the blueberry rhizosphere were compared after inoculation with these different mycorrhizal fungi. The fungal diversity in the blueberry rhizosphere soil was significantly higher after inoculation with DSE, QMK, and ZB than after inoculation with SS and LZ. The dominant class, order, family, and genus of soil fungi were <i>Eurotiomycetes</i> (25.49–36.91%), <i>Eurotiales</i> (24.78–35.00%), <i>Aspergillaceae</i> (24.47–34.74%), and <i>Penicllium</i> (24.34–34.63%), respectively. Among all the fungal treatments, DSE led to the highest abundance of dominant classes, orders, families, and genera. Inoculation with SS, LZ, and DSE increased the contents of nitrogen, phosphorus, and potassium in blueberry plants. The uptake of nitrogen, phosphorus, and potassium were highest in DSE-inoculated plants. The nitrogen content was significantly higher in ZB-inoculated plants than in the control. A TOPSIS analysis showed that among the five fungi, DSE was the best strain to improve the diversity of the blueberry rhizosphere fungal community and promote nutrient uptake by blueberry plants, followed by LZ and ZB. These findings provide scientific data for the development and utilization of soil microbial resources, and provide useful information for improving the cultivation and production of blueberries.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139424017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of gynoecious-parthenocarpic and monoecious cucumber lines (Cucumis sativus L.) and regression modelling to obtain high yielding and functionally rich genotypes","authors":"Suvojit Bose, Soham Hazra, Pranab Hazra, Arup Chattopadhyay, Anirban Maji, Shibnath Basfore, Chandan Karak","doi":"10.1007/s13580-023-00579-y","DOIUrl":"https://doi.org/10.1007/s13580-023-00579-y","url":null,"abstract":"<p>Cucumber (<i>Cucumis sativus</i> L) is a tropical vegetable crop of high economic importance. Cucumber is not only used as food but also the source of several antioxidant nutrients like β carotene and ascorbic acid. The present study was aimed to characterize gynoecious-parthenocarpic and monoecious cucumber lines along with their hybrids for qualitative, quantitative and quality parameters and regression modelling to predict the most sensitive characters influencing yield and nutrient contents (β carotene and ascorbic acid). Characterization revealed conspicuous variation in different qualitative and quantitative characters among the parental lines and hybrids. The gynoecious and parthenocarpic parent Pusa Parthenocarpic produced elongated fruits with acute shaped blossom and peduncle ends while, the other two monoecious and non-parthenocarpic parents, Devgiri and Seven Star produced cylindrical fruit with obtuse ends. All the monoecious × gynoecious hybrids expressed predominantly gynoecious sex form and late parthenocarpy. The monoecious parent recorded higher yield as compared to the gynoecious parent. Acute fruit shape at peduncle end was the sole qualitative character which positively influenced the ascorbic acid content of fruit. Regression modelling revealed fruit length, crispy fruit texture and total chlorophyll content to be most influential towards yield in a positive direction. This study confirmed the strategy of combining qualitative and quantitative characters to enhance yield and nutrient content which can be utilized to develop high yielding and functionally rich cucumber genotypes.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138716559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel edible coating of beeswax impregnated with karonda polyphenol rich extract maintains the chemical and bioactive potential of fresh ber fruit during storage at low temperature","authors":"Karandeep Kaur, Navjot Gupta, Monika Mahajan, Sukhjit Kaur Jawandha, Nirmaljit Kaur","doi":"10.1007/s13580-023-00533-y","DOIUrl":"https://doi.org/10.1007/s13580-023-00533-y","url":null,"abstract":"<p>Ber fruit is highly nutritious and rich in bioactive compounds known to have several health benefits. However, the significant loss of such compounds during long-term storage is a concern. Retaining its quality during post-harvest by natural preservatives could be a novel approach towards sustainability. In the present study, a novel composite edible coating of beeswax and polyphenols was developed. Beeswax, a lipid-based material with anti-transpiration properties, was derived from honeycomb and polyphenols with huge antioxidant and antimicrobial properties were extracted from underutilized fruit, karonda. The purpose of the present work is to evaluate the effect of this composite coating on the storage life of ber fruits. For this, beeswax (2%) alone or impregnated with the polyphenols at different concentrations 0.5% (300 mg/L), 1% (600 mg/L) and 1.5% (750 mg/L) was applied on the fruit's surface. The effect of this composite edible coating on the physicochemical properties, bioactive compounds and antioxidant enzymes activities of ber fruits was studied for 28 days during cold storage. The results revealed that the combination of beeswax coating with karonda polyphenols markedly reduced spoilage and maintained the firmness of ber fruit during storage. The coating has resulted in higher retention of ascorbic acid, flavonoids, phenolics and antioxidant activity during storage. The treated fruits showed delayed ripening by slowing down the synthesis of carotenoids as compared to the control fruit. Further, the coating resulted in the lower activities of cell wall degrading enzymes like pectin methyl esterase and cellulase enzymes in the stored ber fruits. Moreover, the concentrations of karonda polyphenols (KPP) have worked in a dose-dependent manner in the coating system, as beeswax with 1.5% KPP was found to be more effective in maintaining the quality and nutritional potential of ber fruit during storage.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138568359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethylene-driven expression of genes involved in carotenoid biosynthesis during postharvest ripening is different in creole and commercial Carica papaya L. fruits","authors":"Arianna C. Chan-León, Humberto Estrella-Maldonado, Gabriela Fuentes-Ortiz, Luis Torres, Sergio Peraza Sánchez, Jorge M. Santamaría","doi":"10.1007/s13580-023-00568-1","DOIUrl":"https://doi.org/10.1007/s13580-023-00568-1","url":null,"abstract":"<p>This paper reports ethylene production, expression levels of ethylene precursor’s genes, as well as carotenoid content and expression levels of carotenoid biosynthesis genes, during postharvest ripening of three different papaya genotypes of contrasting origin (2 creoles and 1 commercial) and pulp color (yellow, orange and red), under conditions that promote ethylene formation and under those inhibiting ethylene. Our study showed that naturally ripened papaya fruits presented relatively high ethylene production levels that was correlated with changes in expression levels of both ethylene precursor’s genes (CpACCs1, CpACCs2, CpACCo1 and CpACCo2), and carotenoid biosynthesis genes (CpPSY, CpZDS, CpLCY-β2, CpCHX-β). However, when papaya fruits were treated with an ethylene inducer (Ethephon; 2.5 g L^− 1 of 2-chloroethyl-phosphonic acid) fruits ripened faster (two days earlier than controls), and they also showed a higher ethylene peak and higher expression levels of both ethylene precursors genes and carotenoid biosynthesis genes, than control fruits. In contrast, papaya fruits treated with an ethylene inhibitor (1-MCP; 300 nL L^− 1 of 1-methylcyclopropene) never ripened (in fact they did not show changes in fruit firmness, °Brix, pH or acidity), and they showed very low ethylene production and low expression levels of ethylene precursors genes, as well as showing low expression of carotenogenic genes, throughout the postharvest ripening process. Correlation analysis showed that increased ethylene production was highly correlated with increased expression of both ethylene precursors genes and carotenoids biosynthesis genes, but the specific carotenogenic genes induced by ethylene differed among commercial and creole papaya genotypes.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138512425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a novel promoter region responsible for the embryo-specific expression of SERK1 in pineapple","authors":"Tao Xie, Wei Zhang, Chengjie Chen, Xiaoshuang Wang, Jing Zhang, Aiping Luan, Yehua He","doi":"10.1007/s13580-023-00542-x","DOIUrl":"https://doi.org/10.1007/s13580-023-00542-x","url":null,"abstract":"<p>Somatic embryogenesis (SE) is a key regeneration process in plant. <i>AcSERK1</i> is a gene specifically expressed in the early stage of SE in pineapple (<i>Ananas comosus</i>), suggesting that the promoter of <i>SERK1</i> might contain specific cis-acting element regulating SE. To identify embryonic cell-specific element in the <i>SERK1</i> promoter, a series of binary plant transformation vectors with GUS (β-glucuronidase) reporter gene were systematically analyzed by transient gene expression system in wild-type and transgenic pineapple embryogenic callus. Histochemical and quantitative GUS assays demonstrated that the activity of the <i>AcSERK1</i> upstream regulatory sequence lacking − 921 to -911 or -910 to -880 was significantly reduced in the embryonic callus of the pineapple, and these two regions were needed for the embryonic cell-specific. Besides, a promoter lacking − 943 to -922 was shown to significantly increase GUS activity in embryogenic callus, suggesting repressive elements exist in this region. Our data of stable transformation assays confirmed again the 5’ upstream regulatory sequence (-921 to -880) of the <i>AcSERK1</i> gene is an essential functional region. Our findings lay the basis for better understanding of the molecular mechanisms of <i>AcSERK1</i> gene in the regulation in early stage of SE.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138512420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of photosynthetic efficiency and growth characteristics of paprika in semi-closed vs. conventional greenhouses","authors":"Eun ji Kim, Beom Seon Lee, Yong Hwan Jeon, Se Hun Ju, Yoon Go, Jin Sub Park, Joonwoo Lee, Huijun Yang, Haeyoung Na","doi":"10.1007/s13580-023-00575-2","DOIUrl":"https://doi.org/10.1007/s13580-023-00575-2","url":null,"abstract":"<p>This study investigated the differences in light intensity, photosynthetic efficiency, and growth characteristics of paprika plants cultivated in a semi-closed greenhouse covered with low-iron diffuse glass (SG-LDG) and a conventional greenhouse covered with low-iron regular glass (CG-LRG). There was no significant difference in light intensity between the SG-LDG and CG-LRG from the apical meristem to below 100 cm. However, below 150 cm, the SG-LDG showed higher light intensity than the CG-LRG. The light response curve and atmospheric CO₂ response(A-C_a) curve, which represent photosynthetic efficiency, were not significantly affected by crop canopy differences between the two greenhouses during the early cultivation stage. However, after 12 weeks of planting, SG-LDG showed higher photosynthetic efficiency. The J_max and V_cmax values showed no differences between the plants in the two greenhouses until 4 weeks after planting. 12 weeks after planting, plants in SG-LDG showed higher J_max and V_cmax values than those in CG-LRG. All the growth characteristics of plants cultivated in SG-LDG were superior to those of plants cultivated in CG-LRG. Therefore, the improvement in light intensity and photosynthetic efficiency at the lower positions of plants in SG-LDG is considered to have a positive impact on crop quantity and quality.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138543019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and authentication of <i>Panax ginseng</i> cv. Sunhong with high yield and multiple tolerance to heat damage, rusty roots and lodging.","authors":"Jiho Seo,&nbsp;Joon-Soo Lee,&nbsp;Sung-Lye Shim,&nbsp;Jun-Gyo In,&nbsp;Chol-Soo Park,&nbsp;Yong-Jae Lee,&nbsp;Hee-Jun Ahn","doi":"10.1007/s13580-023-00526-x","DOIUrl":"https://doi.org/10.1007/s13580-023-00526-x","url":null,"abstract":"<p><p>Ginseng (<i>Panax ginseng</i>) has been used as a valuable medicinal plant in Asia, and the demand for ginseng production for health functional food is increasing worldwide after the COVID-19 crisis. Although a number of cultivars have been developed to increase ginseng production, none of them were widely cultivated in Korea because they could not resist various environmental stresses while being grown in one place for at least 4 years. To address this, Sunhong was developed as a ginseng cultivar with high yield and multiple stress tolerance by pure line selection. Sunhong showed high yield and heat tolerance comparable to Yunpoong, a representative high-yielding cultivar, and exhibited 1.4 times lower prevalence of rusty roots than Yunpoong, suggesting that Sunhong can keep its high yield and quality during long-term cultivation. In addition, distinct color and lodging resistance were expected to increase the convenience of cultivation. To supply pure seeds to farmers, we also established a reliable high-throughput authentication system for Sunhong and seven ginseng cultivars through genotyping-by-sequencing (GBS) analysis. The GBS approach enabled to identify a sufficient number of informative SNPs in ginseng, a heterozygous and polyploid species. These results contribute to the improvement of yield, quality, and homogeneity, and therefore promote the ginseng industry.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13580-023-00526-x.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10202357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10074241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of the protective antigen for PEDV in transgenic duckweed, <i>Lemna minor</i>.","authors":"Suk-Min Ko,&nbsp;Hyeon-Jin Sun,&nbsp;Myung Jin Oh,&nbsp;In-Ja Song,&nbsp;Min-Jae Kim,&nbsp;Hyun-Sook Sin,&nbsp;Chang-Hyo Goh,&nbsp;Yong-Woo Kim,&nbsp;Pyung-Ok Lim,&nbsp;Hyo-Yeon Lee,&nbsp;Suk Weon Kim","doi":"10.1007/s13580-011-0007-x","DOIUrl":"https://doi.org/10.1007/s13580-011-0007-x","url":null,"abstract":"<p><p>Duckweeds are small, floating aquatic plants with a number of useful characteristics, including edibility, fast-growing, and a clonal proliferation. Duckweed is also fed to animals as a diet complement because of its high nutritional value. Porcine epidemic diarrhea virus (PEDV) is a major causative agent of fatal diarrhea in piglets and is a serious problem in the hog-raising industry. In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1 using duckweed, <i>Lemna minor</i>. Stably transformed <i>Lemna</i> were obtained by co-cultivation with <i>A. tumefaciens</i> EHA105 harboring the PEDV spike protein gene. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic <i>Lemna</i>, respectively. This is the first report of the expression of a vaccine antigen against an animal infectious disease in duckweed.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13580-011-0007-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37783258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}