菠萝SERK1胚胎特异性表达的新启动子区域的鉴定

IF 2.4 3区 农林科学 Q1 Agricultural and Biological Sciences
Tao Xie, Wei Zhang, Chengjie Chen, Xiaoshuang Wang, Jing Zhang, Aiping Luan, Yehua He
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引用次数: 0

摘要

体细胞胚胎发生是植物再生的关键过程。AcSERK1是菠萝(Ananas comosus) SE早期特异性表达的基因,提示SERK1的启动子可能含有特异性的顺式作用元件调控SE。为了鉴定SERK1启动子中的胚胎细胞特异性元件,采用转基因和野生型菠萝胚性愈伤组织瞬时基因表达系统分析了一系列带有GUS (β-葡萄糖醛酸酶)报告基因的二元植物转化载体。组织化学和定量GUS分析表明,缺乏- 921 ~ -911或-910 ~ -880的AcSERK1上游调控序列在菠萝胚胎愈伤组织中活性显著降低,这两个区域是胚胎细胞特异性所必需的。此外,缺失- 943 ~ -922的启动子显著提高了胚性愈伤组织中GUS活性,表明该区域存在抑制因子。我们的稳定转化实验数据再次证实,AcSERK1基因的5 '上游调控序列(-921至-880)是一个重要的功能区域。我们的发现为更好地理解AcSERK1基因在SE早期调控中的分子机制奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Identification of a novel promoter region responsible for the embryo-specific expression of SERK1 in pineapple

Identification of a novel promoter region responsible for the embryo-specific expression of SERK1 in pineapple

Somatic embryogenesis (SE) is a key regeneration process in plant. AcSERK1 is a gene specifically expressed in the early stage of SE in pineapple (Ananas comosus), suggesting that the promoter of SERK1 might contain specific cis-acting element regulating SE. To identify embryonic cell-specific element in the SERK1 promoter, a series of binary plant transformation vectors with GUS (β-glucuronidase) reporter gene were systematically analyzed by transient gene expression system in wild-type and transgenic pineapple embryogenic callus. Histochemical and quantitative GUS assays demonstrated that the activity of the AcSERK1 upstream regulatory sequence lacking − 921 to -911 or -910 to -880 was significantly reduced in the embryonic callus of the pineapple, and these two regions were needed for the embryonic cell-specific. Besides, a promoter lacking − 943 to -922 was shown to significantly increase GUS activity in embryogenic callus, suggesting repressive elements exist in this region. Our data of stable transformation assays confirmed again the 5’ upstream regulatory sequence (-921 to -880) of the AcSERK1 gene is an essential functional region. Our findings lay the basis for better understanding of the molecular mechanisms of AcSERK1 gene in the regulation in early stage of SE.

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来源期刊
Horticulture Environment and Biotechnology
Horticulture Environment and Biotechnology Agricultural and Biological Sciences-Horticulture
CiteScore
4.30
自引率
4.20%
发文量
0
审稿时长
6 months
期刊介绍: Horticulture, Environment, and Biotechnology (HEB) is the official journal of the Korean Society for Horticultural Science, was launched in 1965 as the "Journal of Korean Society for Horticultural Science". HEB is an international journal, published in English, bimonthly on the last day of even number months, and indexed in Biosys Preview, SCIE, and CABI. The journal is devoted for the publication of original research papers and review articles related to vegetables, fruits, ornamental and herbal plants, and covers all aspects of physiology, molecular biology, biotechnology, protected cultivation, postharvest technology, and research in plants related to environment.
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