Arianna C. Chan-León, Humberto Estrella-Maldonado, Gabriela Fuentes-Ortiz, Luis Torres, Sergio Peraza Sánchez, Jorge M. Santamaría
{"title":"采后成熟过程中参与类胡萝卜素生物合成的基因的乙烯驱动表达在克里奥尔和商品番木瓜果实中是不同的","authors":"Arianna C. Chan-León, Humberto Estrella-Maldonado, Gabriela Fuentes-Ortiz, Luis Torres, Sergio Peraza Sánchez, Jorge M. Santamaría","doi":"10.1007/s13580-023-00568-1","DOIUrl":null,"url":null,"abstract":"<p>This paper reports ethylene production, expression levels of ethylene precursor’s genes, as well as carotenoid content and expression levels of carotenoid biosynthesis genes, during postharvest ripening of three different papaya genotypes of contrasting origin (2 creoles and 1 commercial) and pulp color (yellow, orange and red), under conditions that promote ethylene formation and under those inhibiting ethylene. Our study showed that naturally ripened papaya fruits presented relatively high ethylene production levels that was correlated with changes in expression levels of both ethylene precursor’s genes (CpACCs1, CpACCs2, CpACCo1 and CpACCo2), and carotenoid biosynthesis genes (CpPSY, CpZDS, CpLCY-β2, CpCHX-β). However, when papaya fruits were treated with an ethylene inducer (Ethephon; 2.5 g L<sup>− 1</sup> of 2-chloroethyl-phosphonic acid) fruits ripened faster (two days earlier than controls), and they also showed a higher ethylene peak and higher expression levels of both ethylene precursors genes and carotenoid biosynthesis genes, than control fruits. In contrast, papaya fruits treated with an ethylene inhibitor (1-MCP; 300 nL L<sup>− 1</sup> of 1-methylcyclopropene) never ripened (in fact they did not show changes in fruit firmness, °Brix, pH or acidity), and they showed very low ethylene production and low expression levels of ethylene precursors genes, as well as showing low expression of carotenogenic genes, throughout the postharvest ripening process. Correlation analysis showed that increased ethylene production was highly correlated with increased expression of both ethylene precursors genes and carotenoids biosynthesis genes, but the specific carotenogenic genes induced by ethylene differed among commercial and creole papaya genotypes.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.4000,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ethylene-driven expression of genes involved in carotenoid biosynthesis during postharvest ripening is different in creole and commercial Carica papaya L. fruits\",\"authors\":\"Arianna C. Chan-León, Humberto Estrella-Maldonado, Gabriela Fuentes-Ortiz, Luis Torres, Sergio Peraza Sánchez, Jorge M. Santamaría\",\"doi\":\"10.1007/s13580-023-00568-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>This paper reports ethylene production, expression levels of ethylene precursor’s genes, as well as carotenoid content and expression levels of carotenoid biosynthesis genes, during postharvest ripening of three different papaya genotypes of contrasting origin (2 creoles and 1 commercial) and pulp color (yellow, orange and red), under conditions that promote ethylene formation and under those inhibiting ethylene. Our study showed that naturally ripened papaya fruits presented relatively high ethylene production levels that was correlated with changes in expression levels of both ethylene precursor’s genes (CpACCs1, CpACCs2, CpACCo1 and CpACCo2), and carotenoid biosynthesis genes (CpPSY, CpZDS, CpLCY-β2, CpCHX-β). However, when papaya fruits were treated with an ethylene inducer (Ethephon; 2.5 g L<sup>− 1</sup> of 2-chloroethyl-phosphonic acid) fruits ripened faster (two days earlier than controls), and they also showed a higher ethylene peak and higher expression levels of both ethylene precursors genes and carotenoid biosynthesis genes, than control fruits. In contrast, papaya fruits treated with an ethylene inhibitor (1-MCP; 300 nL L<sup>− 1</sup> of 1-methylcyclopropene) never ripened (in fact they did not show changes in fruit firmness, °Brix, pH or acidity), and they showed very low ethylene production and low expression levels of ethylene precursors genes, as well as showing low expression of carotenogenic genes, throughout the postharvest ripening process. Correlation analysis showed that increased ethylene production was highly correlated with increased expression of both ethylene precursors genes and carotenoids biosynthesis genes, but the specific carotenogenic genes induced by ethylene differed among commercial and creole papaya genotypes.</p>\",\"PeriodicalId\":13123,\"journal\":{\"name\":\"Horticulture Environment and Biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2023-11-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Horticulture Environment and Biotechnology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s13580-023-00568-1\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Horticulture Environment and Biotechnology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s13580-023-00568-1","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
摘要
本文报道了不同来源的三种不同基因型(2种克里奥尔和1种商品)和果肉颜色(黄色、橙色和红色)在促进乙烯形成和抑制乙烯形成的条件下,采后成熟过程中乙烯的产量、乙烯前体基因的表达水平、类胡萝卜素含量和类胡萝卜素生物合成基因的表达水平。我们的研究表明,自然成熟的木瓜果实具有较高的乙烯产量,这与乙烯前体基因(CpACCs1、CpACCs2、CpACCo1和CpACCo2)和类胡萝卜素生物合成基因(CpPSY、CpZDS、CpLCY-β2、CpCHX-β)的表达水平变化有关。然而,当木瓜果实用乙烯诱导剂(乙烯利)处理时;2.5 g L−1 2-氯乙基膦酸处理的果实比对照早熟2 d,乙烯峰较高,乙烯前体基因和类胡萝卜素生物合成基因的表达量均高于对照。相比之下,木瓜果实用乙烯抑制剂(1-MCP;300 nL L−1的1-甲基环丙烯)从未成熟(事实上,它们没有表现出果实硬度、白度、pH或酸度的变化),并且在整个采后成熟过程中,它们表现出非常低的乙烯产量和乙烯前体基因的低表达水平,以及胡萝卜素基因的低表达。相关分析表明,乙烯产量的增加与乙烯前体基因和类胡萝卜素生物合成基因的表达增加高度相关,但乙烯诱导的特异性胡萝卜素基因在商品型和克里奥尔型木瓜中存在差异。
Ethylene-driven expression of genes involved in carotenoid biosynthesis during postharvest ripening is different in creole and commercial Carica papaya L. fruits
This paper reports ethylene production, expression levels of ethylene precursor’s genes, as well as carotenoid content and expression levels of carotenoid biosynthesis genes, during postharvest ripening of three different papaya genotypes of contrasting origin (2 creoles and 1 commercial) and pulp color (yellow, orange and red), under conditions that promote ethylene formation and under those inhibiting ethylene. Our study showed that naturally ripened papaya fruits presented relatively high ethylene production levels that was correlated with changes in expression levels of both ethylene precursor’s genes (CpACCs1, CpACCs2, CpACCo1 and CpACCo2), and carotenoid biosynthesis genes (CpPSY, CpZDS, CpLCY-β2, CpCHX-β). However, when papaya fruits were treated with an ethylene inducer (Ethephon; 2.5 g L− 1 of 2-chloroethyl-phosphonic acid) fruits ripened faster (two days earlier than controls), and they also showed a higher ethylene peak and higher expression levels of both ethylene precursors genes and carotenoid biosynthesis genes, than control fruits. In contrast, papaya fruits treated with an ethylene inhibitor (1-MCP; 300 nL L− 1 of 1-methylcyclopropene) never ripened (in fact they did not show changes in fruit firmness, °Brix, pH or acidity), and they showed very low ethylene production and low expression levels of ethylene precursors genes, as well as showing low expression of carotenogenic genes, throughout the postharvest ripening process. Correlation analysis showed that increased ethylene production was highly correlated with increased expression of both ethylene precursors genes and carotenoids biosynthesis genes, but the specific carotenogenic genes induced by ethylene differed among commercial and creole papaya genotypes.
期刊介绍:
Horticulture, Environment, and Biotechnology (HEB) is the official journal of the Korean Society for Horticultural Science, was launched in 1965 as the "Journal of Korean Society for Horticultural Science".
HEB is an international journal, published in English, bimonthly on the last day of even number months, and indexed in Biosys Preview, SCIE, and CABI.
The journal is devoted for the publication of original research papers and review articles related to vegetables, fruits, ornamental and herbal plants, and covers all aspects of physiology, molecular biology, biotechnology, protected cultivation, postharvest technology, and research in plants related to environment.