Horticulture Environment and Biotechnology最新文献_第7页

Identification of a novel promoter region responsible for the embryo-specific expression of SERK1 in pineapple 菠萝SERK1胚胎特异性表达的新启动子区域的鉴定

IF 2.4 3区农林科学

Horticulture Environment and Biotechnology Pub Date : 2023-11-22 DOI: 10.1007/s13580-023-00542-x

Tao Xie, Wei Zhang, Chengjie Chen, Xiaoshuang Wang, Jing Zhang, Aiping Luan, Yehua He

{"title":"Identification of a novel promoter region responsible for the embryo-specific expression of SERK1 in pineapple","authors":"Tao Xie, Wei Zhang, Chengjie Chen, Xiaoshuang Wang, Jing Zhang, Aiping Luan, Yehua He","doi":"10.1007/s13580-023-00542-x","DOIUrl":"https://doi.org/10.1007/s13580-023-00542-x","url":null,"abstract":"Somatic embryogenesis (SE) is a key regeneration process in plant. AcSERK1 is a gene specifically expressed in the early stage of SE in pineapple (Ananas comosus), suggesting that the promoter of SERK1 might contain specific cis-acting element regulating SE. To identify embryonic cell-specific element in the SERK1 promoter, a series of binary plant transformation vectors with GUS (β-glucuronidase) reporter gene were systematically analyzed by transient gene expression system in wild-type and transgenic pineapple embryogenic callus. Histochemical and quantitative GUS assays demonstrated that the activity of the AcSERK1 upstream regulatory sequence lacking − 921 to -911 or -910 to -880 was significantly reduced in the embryonic callus of the pineapple, and these two regions were needed for the embryonic cell-specific. Besides, a promoter lacking − 943 to -922 was shown to significantly increase GUS activity in embryogenic callus, suggesting repressive elements exist in this region. Our data of stable transformation assays confirmed again the 5’ upstream regulatory sequence (-921 to -880) of the AcSERK1 gene is an essential functional region. Our findings lay the basis for better understanding of the molecular mechanisms of AcSERK1 gene in the regulation in early stage of SE.","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":"10 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138512420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of photosynthetic efficiency and growth characteristics of paprika in semi-closed vs. conventional greenhouses 半封闭与常规温室中辣椒光合效率及生长特性的比较

IF 2.4 3区农林科学

Horticulture Environment and Biotechnology Pub Date : 2023-11-20 DOI: 10.1007/s13580-023-00575-2

Eun ji Kim, Beom Seon Lee, Yong Hwan Jeon, Se Hun Ju, Yoon Go, Jin Sub Park, Joonwoo Lee, Huijun Yang, Haeyoung Na

{"title":"Comparison of photosynthetic efficiency and growth characteristics of paprika in semi-closed vs. conventional greenhouses","authors":"Eun ji Kim, Beom Seon Lee, Yong Hwan Jeon, Se Hun Ju, Yoon Go, Jin Sub Park, Joonwoo Lee, Huijun Yang, Haeyoung Na","doi":"10.1007/s13580-023-00575-2","DOIUrl":"https://doi.org/10.1007/s13580-023-00575-2","url":null,"abstract":"This study investigated the differences in light intensity, photosynthetic efficiency, and growth characteristics of paprika plants cultivated in a semi-closed greenhouse covered with low-iron diffuse glass (SG-LDG) and a conventional greenhouse covered with low-iron regular glass (CG-LRG). There was no significant difference in light intensity between the SG-LDG and CG-LRG from the apical meristem to below 100 cm. However, below 150 cm, the SG-LDG showed higher light intensity than the CG-LRG. The light response curve and atmospheric CO2 response(A-Ca) curve, which represent photosynthetic efficiency, were not significantly affected by crop canopy differences between the two greenhouses during the early cultivation stage. However, after 12 weeks of planting, SG-LDG showed higher photosynthetic efficiency. The Jmax and Vcmax values showed no differences between the plants in the two greenhouses until 4 weeks after planting. 12 weeks after planting, plants in SG-LDG showed higher Jmax and Vcmax values than those in CG-LRG. All the growth characteristics of plants cultivated in SG-LDG were superior to those of plants cultivated in CG-LRG. Therefore, the improvement in light intensity and photosynthetic efficiency at the lower positions of plants in SG-LDG is considered to have a positive impact on crop quantity and quality.","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":"316 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138543019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and authentication of Panax ginseng cv. Sunhong with high yield and multiple tolerance to heat damage, rusty roots and lodging. 人参的开发与鉴定。孙红产量高，耐热害、耐锈根、耐倒伏。

IF 2.4 3区农林科学

Horticulture Environment and Biotechnology Pub Date : 2023-05-22 DOI: 10.1007/s13580-023-00526-x

Jiho Seo, Joon-Soo Lee, Sung-Lye Shim, Jun-Gyo In, Chol-Soo Park, Yong-Jae Lee, Hee-Jun Ahn

{"title":"Development and authentication of Panax ginseng cv. Sunhong with high yield and multiple tolerance to heat damage, rusty roots and lodging.","authors":"Jiho Seo, Joon-Soo Lee, Sung-Lye Shim, Jun-Gyo In, Chol-Soo Park, Yong-Jae Lee, Hee-Jun Ahn","doi":"10.1007/s13580-023-00526-x","DOIUrl":"https://doi.org/10.1007/s13580-023-00526-x","url":null,"abstract":"Ginseng (Panax ginseng) has been used as a valuable medicinal plant in Asia, and the demand for ginseng production for health functional food is increasing worldwide after the COVID-19 crisis. Although a number of cultivars have been developed to increase ginseng production, none of them were widely cultivated in Korea because they could not resist various environmental stresses while being grown in one place for at least 4 years. To address this, Sunhong was developed as a ginseng cultivar with high yield and multiple stress tolerance by pure line selection. Sunhong showed high yield and heat tolerance comparable to Yunpoong, a representative high-yielding cultivar, and exhibited 1.4 times lower prevalence of rusty roots than Yunpoong, suggesting that Sunhong can keep its high yield and quality during long-term cultivation. In addition, distinct color and lodging resistance were expected to increase the convenience of cultivation. To supply pure seeds to farmers, we also established a reliable high-throughput authentication system for Sunhong and seven ginseng cultivars through genotyping-by-sequencing (GBS) analysis. The GBS approach enabled to identify a sufficient number of informative SNPs in ginseng, a heterozygous and polyploid species. These results contribute to the improvement of yield, quality, and homogeneity, and therefore promote the ginseng industry.Supplementary information: The online version contains supplementary material available at 10.1007/s13580-023-00526-x.","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":" ","pages":"1-12"},"PeriodicalIF":2.4,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10202357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10074241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Expression of the protective antigen for PEDV in transgenic duckweed, Lemna minor. PEDV保护抗原在转基因小扁豆中的表达。

IF 2.4 3区农林科学

Horticulture Environment and Biotechnology Pub Date : 2011-01-01 Epub Date: 2011-10-28 DOI: 10.1007/s13580-011-0007-x

Suk-Min Ko, Hyeon-Jin Sun, Myung Jin Oh, In-Ja Song, Min-Jae Kim, Hyun-Sook Sin, Chang-Hyo Goh, Yong-Woo Kim, Pyung-Ok Lim, Hyo-Yeon Lee, Suk Weon Kim

{"title":"Expression of the protective antigen for PEDV in transgenic duckweed, Lemna minor.","authors":"Suk-Min Ko, Hyeon-Jin Sun, Myung Jin Oh, In-Ja Song, Min-Jae Kim, Hyun-Sook Sin, Chang-Hyo Goh, Yong-Woo Kim, Pyung-Ok Lim, Hyo-Yeon Lee, Suk Weon Kim","doi":"10.1007/s13580-011-0007-x","DOIUrl":"https://doi.org/10.1007/s13580-011-0007-x","url":null,"abstract":"Duckweeds are small, floating aquatic plants with a number of useful characteristics, including edibility, fast-growing, and a clonal proliferation. Duckweed is also fed to animals as a diet complement because of its high nutritional value. Porcine epidemic diarrhea virus (PEDV) is a major causative agent of fatal diarrhea in piglets and is a serious problem in the hog-raising industry. In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1 using duckweed, Lemna minor. Stably transformed Lemna were obtained by co-cultivation with A. tumefaciens EHA105 harboring the PEDV spike protein gene. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic Lemna, respectively. This is the first report of the expression of a vaccine antigen against an animal infectious disease in duckweed.","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":"52 5","pages":"511"},"PeriodicalIF":2.4,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13580-011-0007-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37783258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25