PEDV保护抗原在转基因小扁豆中的表达。

IF 2.4 3区 农林科学 Q1 Agricultural and Biological Sciences
Suk-Min Ko, Hyeon-Jin Sun, Myung Jin Oh, In-Ja Song, Min-Jae Kim, Hyun-Sook Sin, Chang-Hyo Goh, Yong-Woo Kim, Pyung-Ok Lim, Hyo-Yeon Lee, Suk Weon Kim
{"title":"PEDV保护抗原在转基因小扁豆中的表达。","authors":"Suk-Min Ko,&nbsp;Hyeon-Jin Sun,&nbsp;Myung Jin Oh,&nbsp;In-Ja Song,&nbsp;Min-Jae Kim,&nbsp;Hyun-Sook Sin,&nbsp;Chang-Hyo Goh,&nbsp;Yong-Woo Kim,&nbsp;Pyung-Ok Lim,&nbsp;Hyo-Yeon Lee,&nbsp;Suk Weon Kim","doi":"10.1007/s13580-011-0007-x","DOIUrl":null,"url":null,"abstract":"<p><p>Duckweeds are small, floating aquatic plants with a number of useful characteristics, including edibility, fast-growing, and a clonal proliferation. Duckweed is also fed to animals as a diet complement because of its high nutritional value. Porcine epidemic diarrhea virus (PEDV) is a major causative agent of fatal diarrhea in piglets and is a serious problem in the hog-raising industry. In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1 using duckweed, <i>Lemna minor</i>. Stably transformed <i>Lemna</i> were obtained by co-cultivation with <i>A. tumefaciens</i> EHA105 harboring the PEDV spike protein gene. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic <i>Lemna</i>, respectively. This is the first report of the expression of a vaccine antigen against an animal infectious disease in duckweed.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":"52 5","pages":"511"},"PeriodicalIF":2.4000,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13580-011-0007-x","citationCount":"25","resultStr":"{\"title\":\"Expression of the protective antigen for PEDV in transgenic duckweed, <i>Lemna minor</i>.\",\"authors\":\"Suk-Min Ko,&nbsp;Hyeon-Jin Sun,&nbsp;Myung Jin Oh,&nbsp;In-Ja Song,&nbsp;Min-Jae Kim,&nbsp;Hyun-Sook Sin,&nbsp;Chang-Hyo Goh,&nbsp;Yong-Woo Kim,&nbsp;Pyung-Ok Lim,&nbsp;Hyo-Yeon Lee,&nbsp;Suk Weon Kim\",\"doi\":\"10.1007/s13580-011-0007-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Duckweeds are small, floating aquatic plants with a number of useful characteristics, including edibility, fast-growing, and a clonal proliferation. Duckweed is also fed to animals as a diet complement because of its high nutritional value. Porcine epidemic diarrhea virus (PEDV) is a major causative agent of fatal diarrhea in piglets and is a serious problem in the hog-raising industry. In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1 using duckweed, <i>Lemna minor</i>. Stably transformed <i>Lemna</i> were obtained by co-cultivation with <i>A. tumefaciens</i> EHA105 harboring the PEDV spike protein gene. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic <i>Lemna</i>, respectively. This is the first report of the expression of a vaccine antigen against an animal infectious disease in duckweed.</p>\",\"PeriodicalId\":13123,\"journal\":{\"name\":\"Horticulture Environment and Biotechnology\",\"volume\":\"52 5\",\"pages\":\"511\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2011-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1007/s13580-011-0007-x\",\"citationCount\":\"25\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Horticulture Environment and Biotechnology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s13580-011-0007-x\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2011/10/28 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Horticulture Environment and Biotechnology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s13580-011-0007-x","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2011/10/28 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 25

摘要

浮萍是一种小型、漂浮的水生植物,具有许多有用的特性,包括可食用、快速生长和无性系繁殖。由于浮萍的高营养价值,它也被用作动物饲料的补充。猪流行性腹泻病毒(PEDV)是仔猪致死性腹泻的主要病原体,是养猪业面临的一个严重问题。在这项研究中,我们评估了利用浮萍lena minor生产PEDV刺突蛋白1保护性抗原的可行性。通过与携带PEDV刺突蛋白基因的瘤胃杆菌EHA105共培养,获得了稳定转化的Lemna。转基因Lemna分别通过基因组PCR、RT-PCR和western blot分析证实了PEDV刺突蛋白1基因的整合和表达。这是首次报道在浮萍中表达抗动物传染病的疫苗抗原。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression of the protective antigen for PEDV in transgenic duckweed, Lemna minor.

Duckweeds are small, floating aquatic plants with a number of useful characteristics, including edibility, fast-growing, and a clonal proliferation. Duckweed is also fed to animals as a diet complement because of its high nutritional value. Porcine epidemic diarrhea virus (PEDV) is a major causative agent of fatal diarrhea in piglets and is a serious problem in the hog-raising industry. In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1 using duckweed, Lemna minor. Stably transformed Lemna were obtained by co-cultivation with A. tumefaciens EHA105 harboring the PEDV spike protein gene. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic Lemna, respectively. This is the first report of the expression of a vaccine antigen against an animal infectious disease in duckweed.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Horticulture Environment and Biotechnology
Horticulture Environment and Biotechnology Agricultural and Biological Sciences-Horticulture
CiteScore
4.30
自引率
4.20%
发文量
0
审稿时长
6 months
期刊介绍: Horticulture, Environment, and Biotechnology (HEB) is the official journal of the Korean Society for Horticultural Science, was launched in 1965 as the "Journal of Korean Society for Horticultural Science". HEB is an international journal, published in English, bimonthly on the last day of even number months, and indexed in Biosys Preview, SCIE, and CABI. The journal is devoted for the publication of original research papers and review articles related to vegetables, fruits, ornamental and herbal plants, and covers all aspects of physiology, molecular biology, biotechnology, protected cultivation, postharvest technology, and research in plants related to environment.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信