Future microbiology最新文献_第3页

Ceftazidime-avibactam for the treatment of Pseudomonas aeruginosa: a plain language summary. 头孢他啶-阿维巴坦治疗铜绿假单胞菌：简单的语言总结。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-30 DOI: 10.1080/17460913.2025.2479967

Pattarachai Kiratisin, Marie Kempf, Gregory Stone, Eric Utt

引用次数: 0

Unveiling antifungal resistance and biocide tolerance in clinical isolates of Candida spp. 揭示临床分离念珠菌的抗真菌耐药性和生物杀灭剂耐受性。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-22 DOI: 10.1080/17460913.2025.2480924

Jamile De Paiva Macedo, Aripuana Aranha Sakurada Watanabe, Alessandra Barbosa Ferreira Machado, Cláudio Galuppo Diniz, Vania Lucia Da Silva, Vanessa Cordeiro Dias

{"title":"Unveiling antifungal resistance and biocide tolerance in clinical isolates of Candida spp.","authors":"Jamile De Paiva Macedo, Aripuana Aranha Sakurada Watanabe, Alessandra Barbosa Ferreira Machado, Cláudio Galuppo Diniz, Vania Lucia Da Silva, Vanessa Cordeiro Dias","doi":"10.1080/17460913.2025.2480924","DOIUrl":"10.1080/17460913.2025.2480924","url":null,"abstract":"Aims: Candidiasis, caused by Candida spp. is an opportunistic infection with significant healthcare risks, worsened by trends in antifungal resistance. This study aimed to evaluate the antifungal susceptibility profile, investigate resistance mechanisms, assess efflux pump activity, and examine biocide tolerance in clinical Candida isolates.Methods: A total of 100 Candida isolates from hospitalized and outpatient individuals were analyzed for their antifungal susceptibility profile, molecular resistance mechanisms through PCR, efflux pump activity with the Cartwheel method, and biocide tolerance (sodium hypochlorite, hydrogen peroxide, and benzalkonium chloride), which was assessed by disk diffusion.Results: A high prevalence of resistance (87%) to at least one antifungal was observed, with 47.12% of isolates showing simultaneous multiple resistance to three azole derivatives. The highest antifungal agent resistance was observed for fluconazole (n = 70) and the highest susceptibility for amphotericin B (n = 1). The most common mutation was in the ERG11 gene (n = 38/43.7%). Efflux pump activity was detected in both C. albicans and non-albicans Candida species. Biocide testing revealed a higher tolerance for sodium hypochlorite, with an inhibition zone ranging from 18.25 (4.40) to 34.0 (4.00).Conclusions: This study highlights significant antifungal resistance in Candida spp. particularly to azoles, stressing the need for improved infection control and novel therapeutic strategies.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"457-468"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143676911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prohibitins in infection: potential therapeutic targets. 感染禁止药：潜在的治疗靶点。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-01 Epub Date: 2025-01-29 DOI: 10.1080/17460913.2025.2459530

Nuo Chen, Xiaolan Liu, Lulu Wang, Hui Yu, Fangqian Liu, Mengran Yuan, Qimeng Wang, Tianyi Zhang, Xiaoxiang Zhou, Hua Wang, Zengjun Ji, Hongxing Shen

{"title":"Prohibitins in infection: potential therapeutic targets.","authors":"Nuo Chen, Xiaolan Liu, Lulu Wang, Hui Yu, Fangqian Liu, Mengran Yuan, Qimeng Wang, Tianyi Zhang, Xiaoxiang Zhou, Hua Wang, Zengjun Ji, Hongxing Shen","doi":"10.1080/17460913.2025.2459530","DOIUrl":"10.1080/17460913.2025.2459530","url":null,"abstract":"Prohibitins (PHBs) are members of a highly conserved family of proteins, including prohibitin1 and prohibitin2. These proteins are predominantly localized in mitochondria, the nucleus, and cell membranes, where they play critical roles in mitochondrial biogenesis, apoptosis, immune regulation, and other biological processes. Recent studies have demonstrated that both PHB1 and PHB2 can act as a complex or independently to participate in the pathogen infection process. This review focuses on the regulatory roles of PHB1 and PHB2 in viral, bacterial, parasitic and fungal infections, providing a theoretical basis and innovative perspectives for a comprehensive understanding of the roles and mechanisms of PHB1 and PHB2 in the regulation of microbial infections. Due to exerting multiple functions, PHB proteins have been recognized as a potential target for therapeutic interventions, with the expectation that targeting PHB proteins will provide new strategies for the treatment of infection-related diseases.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"345-355"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trichosporon asahii: emerging challenges in pathogenesis and drug resistance. 朝日毛丝虫病：发病机制和耐药性的新挑战。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-01 Epub Date: 2025-01-27 DOI: 10.1080/17460913.2025.2457858

Hanzhao Zhang, Jingping Zhang

{"title":"Trichosporon asahii: emerging challenges in pathogenesis and drug resistance.","authors":"Hanzhao Zhang, Jingping Zhang","doi":"10.1080/17460913.2025.2457858","DOIUrl":"10.1080/17460913.2025.2457858","url":null,"abstract":"Trichosporon asahii (T. asahii) is an opportunistic pathogenic fungus that often causes severe infections in immunosuppressed patients. Among Trichosporon species, T. asahii is the most pathogenic and lethal species. Current research faces challenges related to unknown pathogenic mechanisms, complex resistance mechanisms, insufficiently rapid and accurate diagnostic methods, and insufficient research on susceptibility to infection. These issues need to be explored in depth. This review summarizes research progress on the origin and classification of T. asahii, its virulence factors and pathogenic mechanisms, epidemiological characteristics, infection modes, diagnostic methods, drug treatment options, and drug resistance mechanisms. Traditional culture combined with molecular biology techniques, such as polymerase chain reaction and gene sequencing, has improved the accuracy and speed of detection. Treatment relies mainly on azole antifungal drugs and amphotericin B; however, patients are facing the problem of drug resistance. New techniques, such as gene knockout and gene sequencing, have identified resistance mechanisms, thus supporting the development of novel antifungal drugs. In summary, an in-depth study of T. asahii will aid in developing more effective diagnostic and therapeutic methods and improve patient prognosis.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"333-343"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Propionylation of Fis K32 in Salmonella enterica serovar Typhi: a key modification affecting pathogenicity. 伤寒沙门氏菌血清型中Fis K32丙酸化：影响致病性的关键修饰。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-01 Epub Date: 2025-01-30 DOI: 10.1080/17460913.2025.2460338

Hao Tang, Ziyang Zhan, Xiucheng Liu, Xinxiang Huang

{"title":"Propionylation of Fis K32 in Salmonella enterica serovar Typhi: a key modification affecting pathogenicity.","authors":"Hao Tang, Ziyang Zhan, Xiucheng Liu, Xinxiang Huang","doi":"10.1080/17460913.2025.2460338","DOIUrl":"10.1080/17460913.2025.2460338","url":null,"abstract":"Aim: This study aims to explore the role of propionylation at the K32 residue of the global regulator Fis in Salmonella enterica serovar Typhi (S. Typhi) and its influence on the pathogenicity of the bacteria.Materials & methods: Bacterial strains were cultured in media with sodium propionate supplementation. The propionylation status of Fis was determined through Western blot and mass spectrometry analyses. The DNA-binding capability of Fis was assessed using EMSA. The invasion and survival capacities of S. Typhi were examined using T84 cells and THP-1 macrophages.Results: Propionylation at the K32 site of Fis was found to down-regulate its DNA-binding ability, leading to a reduction in the invasion and survival of S. Typhi within host cells. The K32Q mutant exhibited significantly decreased invasion and survival capabilities compared to the wild-type and K32R mutant strains.Conclusion: Propionylation of Fis at the K32 residue impacts the pathogenicity of S. Typhi, shedding light on the role of post-translational modifications in bacterial infections.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"295-303"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938949/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cefepime-enmetazobactam: first approved cefepime-β- lactamase inhibitor combination for multi-drug resistant Enterobacterales. 头孢吡肟-恩美唑巴坦：首个批准用于多重耐药肠杆菌的头孢吡肟-β-内酰胺酶抑制剂联合用药。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-01 Epub Date: 2025-02-26 DOI: 10.1080/17460913.2025.2468112

Tanaya Bhowmick, Rafael Canton, Federico Pea, Juan Quevedo, Anne Santerre Henriksen, Jean-François Timsit, Keith S Kaye

{"title":"Cefepime-enmetazobactam: first approved cefepime-β- lactamase inhibitor combination for multi-drug resistant Enterobacterales.","authors":"Tanaya Bhowmick, Rafael Canton, Federico Pea, Juan Quevedo, Anne Santerre Henriksen, Jean-François Timsit, Keith S Kaye","doi":"10.1080/17460913.2025.2468112","DOIUrl":"10.1080/17460913.2025.2468112","url":null,"abstract":"Cefepime-enmetazobactam is a β-lactam/β-lactamase inhibitor (BL/BLI) combination that has demonstrated potent activity against extended spectrum beta-lactamase (ESBL)-producing Enterobacterales, recently approved by the US FDA for the treatment of complicated urinary tract infections (cUTI) and by the European Medicines Agency and the UK Healthcare products Regulatory Agency for the treatment of cUTI, hospital-acquired pneumonia including ventilator-associated pneumonia and bacteremia in adults. Cefepime is a 4th generation cephalosporin with a broad spectrum bactericidal activity and enhanced stability to degradation by chromosomal and plasmid-mediated AmpC cephalosporinases as well as carbapenemase OXA-48 like enzymes. Enmetazobactam is a novel penicillanic acid sulfone β-lactamase inhibitor structurally similar to tazobactam with activity against CTX-M, TEM, and SHV ESBL, and other class A β-lactamases, that restores cefepime activity in vitro and in vivo against ESBLs-producing Enterobacterales. The targeted activity of cefepime-enmetazobactam against ESBLs infections which are resistant to common antibiotics, together with its superior results against piperacillin/tazobactam in a phase 3 trial make this new BL/BLI combination an effective carbapenem sparing option for the treatment of serious infections caused by ESBLs-producing Enterobacterales. In addition, if clinical data are supportive, cefepime-enmetazobactam has a potential role in the treatment of pathogens co-producing OXA-48 like enzymes along with ESBLs.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"277-286"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tracking bla_CTX-M transmission through transposable elements in uropathogenic and commensal E. coli. 追踪blaCTX-M通过尿路致病性和共生大肠杆菌中的转座因子传播。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-01 Epub Date: 2025-01-29 DOI: 10.1080/17460913.2025.2459526

Denyss Guilcazo, Liseth Salinas, Cristina Chavez, Katherine Vasquez, Gabriela I Mendez, Lance B Price, Jay P Graham, Joseph N S Eisenberg, Gabriel Trueba

{"title":"Tracking blaCTX-M transmission through transposable elements in uropathogenic and commensal E. coli.","authors":"Denyss Guilcazo, Liseth Salinas, Cristina Chavez, Katherine Vasquez, Gabriela I Mendez, Lance B Price, Jay P Graham, Joseph N S Eisenberg, Gabriel Trueba","doi":"10.1080/17460913.2025.2459526","DOIUrl":"10.1080/17460913.2025.2459526","url":null,"abstract":"Aim: To investigate the nucleotide sequences associated with transposable elements carrying blaCTX-M allelic variants as potential markers for the transmission of antimicrobial resistance genes between domestic animals, humans and the environment.Materials & methods: We conducted whole-genome sequencing and analyzed the nucleotide sequences of most abundant blaCTX-M allelic variants (blaCTX-M-27, blaCTX-M-55, and blaCTX-M-65) in commensal Escherichia coli (n = 20) from household members in Quito and uropathogenic E. coli (UPEC) (n = 149) isolated from nine clinics in Quito, Ecuador.Results: The Ecuadorian commensal E. coli and UPEC displayed identical nucleotide sequences surrounding the blaCTX-M gene and the synteny was similar to those found in other parts of the world; however phylogenetic analysis indicated that the genetic environments in Ecuadorian isolates were unique.Conclusion: These findings suggest that the nucleotide sequences flanking the blaCTX-M genes may be useful for resolving ARG transmission pathways, especially inter-regional analyses.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"287-293"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of nitrofuran derivatives against biofilms of Histoplasma capsulatum strains and their in vivo toxicity. 硝基呋喃衍生物对荚膜组织浆菌生物膜的抑制作用及其体内毒性。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-01 Epub Date: 2025-02-05 DOI: 10.1080/17460913.2025.2457286

Carolina Orlando Vaso, Níura Madalena Bila, Rosângela Aparecida Moraes da Silva, Angélica Romão de Carvalho, Jennyfie Araújo Belizário, Fabiana Pandolfi, Daniela De Vita, Martina Bortolami, Maria José Soares Mendes-Giannini, Luigi Scipione, Roberto Di Santo, Roberta Costi, Caroline Barcelos Costa-Orlandi, Ana Marisa Fusco-Almeida

{"title":"Efficacy of nitrofuran derivatives against biofilms of Histoplasma capsulatum strains and their in vivo toxicity.","authors":"Carolina Orlando Vaso, Níura Madalena Bila, Rosângela Aparecida Moraes da Silva, Angélica Romão de Carvalho, Jennyfie Araújo Belizário, Fabiana Pandolfi, Daniela De Vita, Martina Bortolami, Maria José Soares Mendes-Giannini, Luigi Scipione, Roberto Di Santo, Roberta Costi, Caroline Barcelos Costa-Orlandi, Ana Marisa Fusco-Almeida","doi":"10.1080/17460913.2025.2457286","DOIUrl":"10.1080/17460913.2025.2457286","url":null,"abstract":"Aim: To evaluate the efficacy of two nitrofuran derivatives against biofilms formed by two strains of Histoplasma capsulatum and to study the toxicity of these compounds in alternative models: Caenorhabditis elegans, Galleria mellonella, and zebrafish.Methods: The metabolic activity of biofilms was measured after treatment using the XTT reduction assay. Scanning electron microscopy (SEM) and confocal microscopy were used to observe damage to mature biofilms. Survival curves were generated for G. mellonella, while percentage survival was determined for C. elegans and zebrafish.Results: The compounds showed efficacy against early and mature biofilms at concentrations equal to or up to two times higher than those required to eliminate planktonic fungal cells (3.90 to 31.25 μg/mL). Micrographs showed a reduction in metabolic activity, biofilm thickness, and extracellular matrix. In addition, the compounds showed little or no toxicity in alternative models, even at the highest concentrations tested.Conclusion: These results are promising for the development of new therapeutic alternatives, especially for species, such as H. capsulatum, which are recognized as high-priority pathogens. Few studies have investigated resistance and antifungal treatment targeting biofilms of this species, making this work a relevant contribution to future approaches.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"305-314"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11951717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plain language summary: efficacy and safety of gepotidacin, a new oral antibiotic, compared with nitrofurantoin, a commonly used oral antibiotic, for treating uncomplicated urinary tract infection. 摘要：比较新型口服抗生素吉波替达星与常用口服抗生素呋喃妥因治疗无并发症尿路感染的疗效和安全性。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-01 Epub Date: 2025-02-23 DOI: 10.1080/17460913.2025.2460387

Florian Wagenlehner, Caroline R Perry, Thomas M Hooton, Nicole E Scangarella-Oman, Helen Millns, Salim Janmohamed, Melissa Kramer, Pamela Kushner, Marcy Powell, Emily Jarvis, Jeremy Dennison, Amanda Sheets, Deborah Butler, John Breton

引用次数: 0

Properties and antibacterial effectiveness of metal-ion doped borate-based bioactive glasses. 金属离子掺杂硼酸盐基生物活性玻璃的性能及抗菌效果。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-01 Epub Date: 2025-03-13 DOI: 10.1080/17460913.2025.2470029

Sarah Fakher, David Westenberg

{"title":"Properties and antibacterial effectiveness of metal-ion doped borate-based bioactive glasses.","authors":"Sarah Fakher, David Westenberg","doi":"10.1080/17460913.2025.2470029","DOIUrl":"10.1080/17460913.2025.2470029","url":null,"abstract":"Bioactive glasses (BGs) are physiologically reactive surface biomaterials widely used in biomedical applications and various treatments. Borate bioactive glasses (BBGs) are third-generation BGs, and they exhibit superior biodegradable, bioactive, osteoconductive, antibacterial, and biocompatible properties compared to other types of BGs. Certain concentrations of dopant ions can be incorporated into the chemical structure of BBGs to enhance their biological functionalities and antimicrobial properties. It was demonstrated that those ions play a crucial role in the biological responsiveness in vitro and in vivo once in contact with a physiological environment. The dissolution products of ion-doped BBGs were noted in their ability to stimulate gene expression related to cell differentiation and proliferation, promote angiogenesis, display anti-inflammatory effects, and inhibit bacterial growth within a few hours. Thus, metal-ion-doped BBGs address several limitations encountered by biomedical, tissue engineering, and infection control applications. Considering the research studies on BBGs to date, this review aims to analyze metal-ion-doped BBGs based on their primary antibacterial properties and effectiveness.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"315-331"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0