Future microbiology最新文献

Functional efficacy of Enterococcus faecium HN4 and Lactobacillus delbrueckii HN5 strains isolated from human milk.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-28 DOI: 10.1080/17460913.2025.2484924

Harsha Nirvan, Garima Deswal, Manjit K Selwal, Krishan Kumar Selwal

引用次数: 0

Resistance genes are not like chemical pollutants and surveillance of them should reflect that.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-10 DOI: 10.1080/17460913.2025.2476880

Patrick Munk, Christian Brinch, Frank M Aarestrup

引用次数: 0

Sodium ibuprofenate: antibacterial activities and potential β-lactamase inhibition in critical Gram-negative bacteria.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-09 DOI: 10.1080/17460913.2025.2475639

Franco A Manzanelli, Camila M Clemente, Luciana P Campagno, Dante M Beltramo, Sara M Robledo, Soledad Ravetti, Ariel G Garro

{"title":"Sodium ibuprofenate: antibacterial activities and potential β-lactamase inhibition in critical Gram-negative bacteria.","authors":"Franco A Manzanelli, Camila M Clemente, Luciana P Campagno, Dante M Beltramo, Sara M Robledo, Soledad Ravetti, Ariel G Garro","doi":"10.1080/17460913.2025.2475639","DOIUrl":"10.1080/17460913.2025.2475639","url":null,"abstract":"Aims: To evaluate the antibacterial and antibiofilm activities of sodium ibuprofenate (NaI) and its hypertonic variant (NaIHS) against multidrug-resistant Gram-negative bacteria (MDR-GNB) and explore their potential to inhibit β-lactamase enzymes.Methods: Antibacterial activity was assessed using minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and time-kill assays. Antibiofilm activity was evaluated by measuring bacterial viability and biomass reduction in preformed biofilms. Scanning electron microscopy (SEM) was used to observe membrane effects. Molecular docking and molecular dynamics simulations were conducted to analyze the binding affinity of ibuprofen to the active sites of β-lactamases (CTX-M-15, KPC-2, OXA-23).Results: NaI exhibited bactericidal activity at concentrations of 25-75 mm, with Acinetobacter baumannii being the most susceptible. NaCl (≥0.5 M) enhanced bactericidal efficacy and lowered MBCs. Time-kill assays indicated rapid bacterial eradication within 2 hours, with NaIHS achieving similar results at lower concentrations. SEM confirmed membrane disruption. Both formulations reduced bacterial viability in biofilms, with NaIHS showing greater efficiency. In silico studies suggest ibuprofen may inhibit β-lactamases, with enhanced interactions in saline environments.Conclusion: Sodium ibuprofenate, particularly in its hypertonic form, demonstrates strong antibacterial, antibiofilm, and potential β-lactamase inhibitory activity, making it a promising candidate for treating MDR-GNB infections.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"395-407"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reviving the past for a healthier future: ancient molecules and remedies as a solution to the antibiotic crisis.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-18 DOI: 10.1080/17460913.2025.2476290

Miguel Ángel Díaz-Guerrero, Israel Castillo-Juárez, Rimma Zurabian, Alejandra Valdez, Kokila Kota, Yuki Hoshiko, Ekaprana Ramesh, Mariano Martínez-Vazquez, Corina Diana Ceapă, Mariel Hernandez-Garnica, Frederic Cadet, Rodolfo García-Contreras

{"title":"Reviving the past for a healthier future: ancient molecules and remedies as a solution to the antibiotic crisis.","authors":"Miguel Ángel Díaz-Guerrero, Israel Castillo-Juárez, Rimma Zurabian, Alejandra Valdez, Kokila Kota, Yuki Hoshiko, Ekaprana Ramesh, Mariano Martínez-Vazquez, Corina Diana Ceapă, Mariel Hernandez-Garnica, Frederic Cadet, Rodolfo García-Contreras","doi":"10.1080/17460913.2025.2476290","DOIUrl":"10.1080/17460913.2025.2476290","url":null,"abstract":"Options to combat bacterial infections are becoming scarce. We require innovative approaches to enhance the discovery of effective antimicrobials capable of combating bacteria resistant to multiple or all antibiotics. These methods should either directly eliminate resistant bacteria or indirectly influence their viability by inhibiting their virulence or reducing their resistance to antibiotics. One interesting approach is to analyze ancient remedies used to treat bacterial infections, formulate them, and test them against modern microbes. This field has recently been named \"ancientbiotics.\" This approach allows us to leverage centuries of empirical knowledge accumulated, from traditional medicines across various ancient cultures worldwide. The strategy has already yielded promising formulations to combat the ESKAPE group of nosocomial pathogens. Additionally, molecular de-extinction, which involves genome analysis of extinct species to search for useful antimicrobials, such as peptides, offers another avenue. In this review, we compile the antimicrobial effects of ancient remedies and de-extinct molecules known to modern science and discuss possible new strategies to further harness the potential of past remedies and molecules to fight the rise of superbugs.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"429-441"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface display of LukF-PV on Bacillus subtilis spore for oral administration.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-21 DOI: 10.1080/17460913.2025.2480933

Nhi Ny Nguyen, Lan Duong, Minh B Doan, Hoang Duc Nguyen

{"title":"Surface display of LukF-PV on Bacillus subtilis spore for oral administration.","authors":"Nhi Ny Nguyen, Lan Duong, Minh B Doan, Hoang Duc Nguyen","doi":"10.1080/17460913.2025.2480933","DOIUrl":"10.1080/17460913.2025.2480933","url":null,"abstract":"Aim: Bacillus subtilis spores are promising candidates for oral vaccine, serving as carriers for foreign proteins from pathogens. Here we utilized CotB, a spore coat protein, to anchor the LukF-PV from Staphylococcus aureus onto the surface of B. subtilis spores. Our goal was to generate a new B. subtilis strain and assess the capacity of the recombinant spore strain to induce antibody production in mice.Methods: The fusion sequence of cotB-lukF-PV was cloned into E. coli and transformed into B. subtilis HT800F. Colony PCR confirmed the generation of the recombinant strain. SporeELISA verified the display of LukF-PV. Mice were orally gavaged with the spore, and the production of IgA antibodies in feces and IgG in blood was evaluated.Results: We generated a new B. subtilis strain, BsHT2332, that integrated the PcotB-cotB-lukF-PV into its genome at amyE locus. BsHT2332 successfully displayed LukF-PV on the spore surface. The recombinant spores induced significant IgA and IgG immune responses in mice.Conclusion: This study demonstrated that B. subtilis spores expressing the S. aureus antigen LukF-PV can induce an immune response. These findings underscore the potential of the B. subtilis spore platform as a promising approach for vaccine development against S. aureus.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"469-477"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A family cluster of Brucella abortus infections possibly due to contact with a sika deer in Northeast China.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-19 DOI: 10.1080/17460913.2025.2479297

Kun Li, Dawei Cheng, Zhifeng Xing, Yu Fan, Qingqing Xu, Xueying Chen, Meinan Li, Hongyan Zhao, Dongri Piao, Hai Jiang

引用次数: 0

Correction.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-02-05 DOI: 10.1080/17460913.2025.2463265

引用次数: 0

Modelling the effects of temperature, pH and osmotic shifts on the autofluorescence of Staphylococcus aureus in vitro.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-11 DOI: 10.1080/17460913.2025.2476875

Ao Du, Xiaofen Sun, Meili Dong, Yong Liu, Mingwei Chen, Yuhan Wang, Yuanzhi Zhang, Yao Huang, Zhongsheng Li, Xiang Huang, Yikun Wang, Jingshu Ni

{"title":"Modelling the effects of temperature, pH and osmotic shifts on the autofluorescence of Staphylococcus aureus in vitro.","authors":"Ao Du, Xiaofen Sun, Meili Dong, Yong Liu, Mingwei Chen, Yuhan Wang, Yuanzhi Zhang, Yao Huang, Zhongsheng Li, Xiang Huang, Yikun Wang, Jingshu Ni","doi":"10.1080/17460913.2025.2476875","DOIUrl":"10.1080/17460913.2025.2476875","url":null,"abstract":"Aims: This study aims to investigate how different wound microenvironmental factors (temperature, pH, and osmotic pressure) influence the autofluorescence of Staphylococcus aureus (S. aureus) and its underlying molecular mechanisms, specifically focusing on the porphobilinogen synthase gene (hemB) gene expression.Methods: We measured the average fluorescence intensity of S. aureus colonies under varying conditions of pH (3, 5, 7, 9, 11), temperature (25°C, 31°C, 37°C, 43°C), and osmotic pressure (0.9%, 1.8%, 2.7%, 3.6%) over time. Fluorescence intensity was quantified using ImageJ software. Additionally, RT-qPCR was used to analyze the expression levels of the hemB under these conditions.Results: Bacterial fluorescence intensity increased as the temperature ranged from 25°C to 43°C, with corresponding upregulation of hemB expression. At pH values between 3 and 11, fluorescence intensity decreased as pH increased, reflecting a similar trend in hemB expression. Fluorescence also diminished with higher osmotic pressures (0.9% to 3.6%), mirroring the downregulation of hemB.Conclusions: Our findings indicate that temperature, pH, and osmotic pressure significantly affect the autofluorescence of S. aureus by modulating porphyrin accumulation through hemB gene expression. These environmental factors should be considered when using bacterial fluorescence for wound infection assessment.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"409-418"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Not all disinfectants are created equal: the importance of mechanistic understanding to drive research forward. 并非所有消毒剂都是一样的：从机制上理解推动研究向前发展的重要性。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-17 DOI: 10.1080/17460913.2025.2480946

Elise L Bezold, Kevin P C Minbiole, William M Wuest

引用次数: 0

Does Cryptococcus neoformans infection alter antifungal distribution: an animal model exploring pharmacokinetic changes.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-04-03 DOI: 10.1080/17460913.2025.2484956

Izabel Almeida Alves, Keli Jaqueline Staudt, Bruna Gaelzer Silva Torres, Calinca Elioterio Oliveira, Ryan Lago Araujo Rieper, Layza Kowalski, Bibiana Verlindo de Araújo

{"title":"Does Cryptococcus neoformans infection alter antifungal distribution: an animal model exploring pharmacokinetic changes.","authors":"Izabel Almeida Alves, Keli Jaqueline Staudt, Bruna Gaelzer Silva Torres, Calinca Elioterio Oliveira, Ryan Lago Araujo Rieper, Layza Kowalski, Bibiana Verlindo de Araújo","doi":"10.1080/17460913.2025.2484956","DOIUrl":"10.1080/17460913.2025.2484956","url":null,"abstract":"Aim: Assessing the disseminated meningitis caused by Cryptoccocus neoformans in Wistar rats and its impact on antifungal distribution by microdialysis (µD).Materials & methods: The yeast presence was investigated in different tissues by histological and microbiological assays, and biochemical parameters such as urea, glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), creatinine, creatine kinase (CK), creatinine, albumin level, leukocyte counts, and brain vascular permeability (Evans blue test) were evaluated in healthy and infected groups. Levels of fluconazole reached in the animal's brain were determined by µD.Results: Differences in albumin, urea, GPT, and CK between healthy and infected animals were observed in the levels of Evans blue as well as in the brain (0.51 vs 1.50 µg/gbrain). The drugs' distribution in the brain of infected animals was higher than that in the brain of healthy ones (ft = 1.37 vs ft = 0.54).Conclusion: The model validated presents characteristics similar to those observed in patients and can be applied to pharmacokinetic investigations.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"489-498"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0