Future microbiology最新文献

Activity of extracts and isolated compounds Trichilia catigua against clinically relevant candida species.

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-12-22 DOI: 10.1080/17460913.2024.2444163

Mariane Roberta Ritter, Daniella Renata Faria, Franciele Abigail Vilugron Rodrigues, Danielle Lazarin-Bidóia, Daniela Cristina de Medeiros Araújo, Flavio Augusto Vicente Seixas, Érika Seki Kioshima, João Carlos Palazzo de Mello

{"title":"Activity of extracts and isolated compounds Trichilia catigua against clinically relevant candida species.","authors":"Mariane Roberta Ritter, Daniella Renata Faria, Franciele Abigail Vilugron Rodrigues, Danielle Lazarin-Bidóia, Daniela Cristina de Medeiros Araújo, Flavio Augusto Vicente Seixas, Érika Seki Kioshima, João Carlos Palazzo de Mello","doi":"10.1080/17460913.2024.2444163","DOIUrl":"https://doi.org/10.1080/17460913.2024.2444163","url":null,"abstract":"Objectives: To evaluate the in vitro antifungal activity of extracts and compounds from Trichilia catigua against clinically relevant Candida species, notably Candida glabrata, and investigate possible mechanisms of action using electron microscopy and in silico techniques.Methods: Extracts and fractions of T. catigua were obtained through turboextraction and partitioning, while the isolated compounds were previously purified. The ethyl acetate fraction (EAF) was characterized by HPLC. Antifungal activity against C. glabrata was evaluated through MIC tests, synergism was assessed via checkerboard assays, and structural changes were analyzed via electron microscopy. Molecular docking was performed to identify potential targets of action.Results: The EAF and isolated compounds (cinchonains and procyanidin B2) exhibited significant activity against C. glabrata, with MICs of 9.76 µg/mL (EAF) and 3.9 µg/mL (cinchonains Ia and Ib). Cinchonain Ib combined with epicatechin or procyanidin B2 displayed synergistic effects, particularly with amphotericin B. Microscopy analysis revealed cell membrane damage, and reverse docking analysis suggested that the compounds may target an enzyme essential to the metabolic processes of C. glabrata.Conclusions: The findings suggest that compounds isolated from T. catigua hold considerable potential for developing new antifungal agents against Candida species, particularly C. glabrata, with promising safety and synergistic profiles.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-9"},"PeriodicalIF":2.5,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in the gut microbiota and derived fecal metabolites may play a role in tacrolimus-induced diabetes in mice.

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-12-22 DOI: 10.1080/17460913.2024.2444761

Minyan Qian, Zhenwei Jiang, Caomei Xu, Liying Wang, Nan Hu

{"title":"Changes in the gut microbiota and derived fecal metabolites may play a role in tacrolimus-induced diabetes in mice.","authors":"Minyan Qian, Zhenwei Jiang, Caomei Xu, Liying Wang, Nan Hu","doi":"10.1080/17460913.2024.2444761","DOIUrl":"https://doi.org/10.1080/17460913.2024.2444761","url":null,"abstract":"Aims: A notable scarcity of research has focused on examining alterations in gut microbiota and its metabolites within tacrolimus (TAC)-induced diabetes models.Methods: Tacrolimus-induced changes in glucose and lipid metabolism indices were analyzed through different routes of administration. The potential role of gut microbiota and its metabolites in TAC-induced diabetes was investigated using 16S rRNA sequencing and non-targeted metabolomics.Results: After intraperitoneal(ip) and oral(po) administration of TAC, the α-diversity index of gut microbiota was significantly increased. The gut microbiota of the three groups of mice was significantly separated, and there were significant changes in composition and functional genes. Fecal metabolites changed significantly after TAC administration by different routes, and 53 metabolites (38 down-regulated and 15 up-regulated) were identified (CON vs. TACip). Similarly, 29 metabolites (8 down-regulated and 21 up-regulated) were identified (CON vs. TACpo). KEGG pathway analysis identified 4 and 13 significantly altered metabolic pathways, respectively. Correlation analysis suggested that microbiota and metabolites were involved in the pathogenesis of TAC-induced diabetes.Conclusion: This study investigated the alterations in gut microbiota and fecal metabolites in TAC-induced diabetic mice and evaluated the correlation between these changes. These findings provide valuable insights into potential biomarkers in the development of TAC-induced diabetes.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-10"},"PeriodicalIF":2.5,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel inhibitory strategy of Leishmania major using Kluyveromyces lactis and Saccharomyces cerevisiae killer toxins. 利用乳酸克鲁维酵母菌和酿酒酵母杀手毒素抑制大利什曼病菌的新策略。

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-12-20 DOI: 10.1080/17460913.2024.2443329

Azadeh Zolfaghari, Keivan Beheshti-Maal, Ali Mohammad Ahadi, Ramesh Monajemi

{"title":"A novel inhibitory strategy of Leishmania major using Kluyveromyces lactis and Saccharomyces cerevisiae killer toxins.","authors":"Azadeh Zolfaghari, Keivan Beheshti-Maal, Ali Mohammad Ahadi, Ramesh Monajemi","doi":"10.1080/17460913.2024.2443329","DOIUrl":"https://doi.org/10.1080/17460913.2024.2443329","url":null,"abstract":"Aim: Leishmaniasis is a globally prevalent parasitic disease that has drawn significant attention. Killer yeasts offer a novel biological control method, presenting a potential alternative for treating leishmaniasis. This study evaluates the antileishmanial activity of Kluyveromyces lactis and Saccharomyces cerevisiae killer toxins against Leishmania major.Materials & methods: Killer yeasts were isolated using the Well method. The genes encoding K2 and K.L killer toxins were identified by PCR, and the toxins were purified via SDS-PAGE. Antileishmanial and cytotoxic effects on L. major promastigotes and amastigotes were evaluated using the MTT assay.Results: The first killer isolate was identified as Saccharomyces cerevisiae ZBAM (GenBank accession: OQ376749.1) and the second as Kluyveromyces lactis ZBAM (GenBank accession: OQ401036.1). IC50 values of K2 and K.L toxins against L. major promastigotes were significantly lower than Glucantime and Amphotericin B. The EC50 values at 24 hours for Glucantime, K2, and K.L were 11.83 ± 0.02 μg/ml, 2.35 ± 0.01 μg/ml, and 3.23 ± 0.03 μg/ml, respectively. The EC50 values for K2 and K.L against L. major amastigotes were also lower than Glucantime.Conclusion: This is the first report of the antileishmanial effects of K2 and K.L toxins against L. major, suggesting these yeasts as promising candidates for biological leishmaniasis treatment.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-11"},"PeriodicalIF":2.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First case report of Corynebacterium minutissimum septic arthritis associated with an indwelling device.

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-12-20 DOI: 10.1080/17460913.2024.2444160

Samiha Akkaya, Yusuf Emre Ozdemir, Esra Ensaroglu, Ergun Cetin, Hayat Kumbasar Karaosmanoglu

{"title":"First case report of Corynebacterium minutissimum septic arthritis associated with an indwelling device.","authors":"Samiha Akkaya, Yusuf Emre Ozdemir, Esra Ensaroglu, Ergun Cetin, Hayat Kumbasar Karaosmanoglu","doi":"10.1080/17460913.2024.2444160","DOIUrl":"https://doi.org/10.1080/17460913.2024.2444160","url":null,"abstract":"Corynebacterium minutissimum usually presents with erythrasma, a macular rash, but rarely it can cause extracutaneous infections such as endocarditis, peritonitis, pyelonephritis, and catheter-related bloodstream infections. Here, we present the first case of septic arthritis caused by C. minutissimum and a literature review of invasive C. minutissimum infections. The diagnosis was confirmed by the patient's clinical findings and microbiological examination of the joint fluid. Considering the patients hemodialysis catheter and the pathogenic nature of the C. minutissimum, we strongly suspected a catheter-related infection. Clinical and laboratory follow-ups showed consistent improvement with treatment, characterized by reduced pain, swelling, and localized warmth in the left knee, along with a significant decline in acute-phase reactants. The patient achieved full recovery following a four-week course of antibiotics and catheter removal. Our report emphasizes the importance of not disregarding the isolation of non-diphtheria Corynebacterium from appropriately collected sterile samples in symptomatic patients, particularly those with indwelling devices. Based on this case report, clinicians should consider the rare possibility of this microorganism being the cause of septic arthritis.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-6"},"PeriodicalIF":2.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex and gender in rhinosinusitis: a review.

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-12-14 DOI: 10.1080/17460913.2024.2441010

C S Thornton, L Radu, N Boechler, J Clark, R Somayaji

引用次数: 0

Targeted enzymes delivered by liposomes could address an unmet need in mycobacterial respiratory infections.

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-12-08 DOI: 10.1080/17460913.2024.2423558

Jason W Holder, Richard A Slayden

引用次数: 0

The dual role of microbes in food safety and human health: from pathogens to probiotics.

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-12-05 DOI: 10.1080/17460913.2024.2437273

Helen Onyeaka, Olumide Odeyemi

引用次数: 0

A plain language summary of the STRIVE and ReSTORE studies, which tested if rezafungin is effective and as safe as caspofungin at treating people with candidaemia and invasive candidiasis.

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-12-04 DOI: 10.1080/17460913.2024.2426387

George R Thompson, Alex Soriano, Oliver A Cornely, Jalal A Aram, Peter G Pappas

引用次数: 0

Detection of MPT-64 protein in pleural tuberculosis cases by magnetic bead-gold nanoparticle-PCR amplified immunoassay.

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-11-29 DOI: 10.1080/17460913.2024.2432179

Aishwarya Soni, Kiran Nehra, Bhawna Dahiya, Anam Rais, Tulika Prasad, Anjum Gahlaut, Vikas Raj, Reetu Sheoran, Aparna Parmar, Promod K Mehta

{"title":"Detection of MPT-64 protein in pleural tuberculosis cases by magnetic bead-gold nanoparticle-PCR amplified immunoassay.","authors":"Aishwarya Soni, Kiran Nehra, Bhawna Dahiya, Anam Rais, Tulika Prasad, Anjum Gahlaut, Vikas Raj, Reetu Sheoran, Aparna Parmar, Promod K Mehta","doi":"10.1080/17460913.2024.2432179","DOIUrl":"10.1080/17460913.2024.2432179","url":null,"abstract":"Aim: Diagnosis of pleural tuberculosis (TB) is challenging; thus, an efficient method is urgently needed.Methods: We developed a magnetic-bead-gold nanoparticle-PCR amplified immunoassay (MB-AuNP-I-PCR, liquid system) to detect the Mycobacterium tuberculosis MPT-64 protein in pleural TB patients. AuNPs functionalized with detection antibodies/oligonucleotides were characterized by UV-vis spectroscopy, Transmission/Scanning electron microscopy, Fourier-transform infrared spectrometer, ELISA, and PCR, whereas MBs conjugated with detection antibodies were validated by magneto-ELISA/UV-vis spectroscopy.Results: We utilized the MB-AuNP-I-PCR for MPT-64 detection in 99 clinical specimens which displayed 85.2% sensitivity and 97.8% specificity to diagnose pleural TB cases. Markedly, the sensitivity achieved by MB-AuNP-I-PCR was noticeably higher (p < 0.01) than magneto-ELISA and GeneXpert.Conclusion: This is a preliminary report to diagnose pleural TB cases by MB-AuNP-I-PCR with promising results that require further corroboration in a higher number of specimens.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-9"},"PeriodicalIF":2.5,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multidrug-resistant keratinolytic dermatophytes and non-dermatophytes causing onychomycosis in outpatients. 门诊病人中引起甲癣的耐多药角化溶解性皮癣菌和非皮癣菌。

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-11-26 DOI: 10.1080/17460913.2024.2428140

Sajeda Akter, Parveen Afroz Chowdhury, Marufatuzzahan, Al Hakim, Mehejabin Nurunnahar, Md Asraful Jahan, Md Siraj Uddin, Abul Kalam Azad

{"title":"Multidrug-resistant keratinolytic dermatophytes and non-dermatophytes causing onychomycosis in outpatients.","authors":"Sajeda Akter, Parveen Afroz Chowdhury, Marufatuzzahan, Al Hakim, Mehejabin Nurunnahar, Md Asraful Jahan, Md Siraj Uddin, Abul Kalam Azad","doi":"10.1080/17460913.2024.2428140","DOIUrl":"https://doi.org/10.1080/17460913.2024.2428140","url":null,"abstract":"Aims: This study identified and determined antibiograms of keratinolytic dermatophytes (DM), non-dermatophytic molds (NDM), and yeasts causing onychomycosis.Methods: Morphological, cultural, and biochemical characteristics were used to identify DM and NDM. The keratinolytic activity (KA) and antibiograms were conducted with keratin azure and the agar diffusion method, respectively. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were determined using the microdilution method.Results: Onychomycosis was more prevalent in males (53%) than females, toenails (57%) than fingernails, and commercial employees (40%) than other employees or unemployed. Fungal growth was observed in 92.5% nail samples. DM, NDM, and yeasts caused 46%, 35%, and 19% onychomycosis, respectively. Trichophyton rubrum and Trichophyton mentagrophytes were the common DM. Five different genus of NDM and three different yeasts were isolated. The KA of DM was 30-45% higher than that of NDM and yeasts. All fungal isolates (FI) were resistant to griseofulvin and fluconazole. However, 71%, 64%, and 36% of FI were sensitive to terbinafine hydrochloride, nystatin, and ketoconazole, respectively, while 84% of DM and 46% of NDM were multidrug-resistant. The MIC and MFC of these antifungals against FI ranged from micrograms to milligrams.Conclusion: Multidrug resistance is growing in keratinolytic DM and NDM.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-11"},"PeriodicalIF":2.5,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142727980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0