Future microbiology最新文献

Ceftazidime-avibactam for the treatment of Pseudomonas aeruginosa: a plain language summary.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-30 DOI: 10.1080/17460913.2025.2479967

Pattarachai Kiratisin, Marie Kempf, Gregory Stone, Eric Utt

引用次数: 0

Functional efficacy of Enterococcus faecium HN4 and Lactobacillus delbrueckii HN5 strains isolated from human milk.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-28 DOI: 10.1080/17460913.2025.2484924

Harsha Nirvan, Garima Deswal, Manjit K Selwal, Krishan Kumar Selwal

引用次数: 0

Multidrug-resistant hypervirulent Klebsiella pneumoniae: an evolving superbug.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-26 DOI: 10.1080/17460913.2025.2482478

Yu Zheng, Xiaojue Zhu, Chao Ding, Weiqiang Chu, Xiaoxiao Pang, Ruxia Zhang, Jiucheng Ma, Guoxin Xu

引用次数: 0

Unveiling antifungal resistance and biocide tolerance in clinical isolates of Candida spp.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-22 DOI: 10.1080/17460913.2025.2480924

Jamile De Paiva Macedo, Aripuana Aranha Sakurada Watanabe, Alessandra Barbosa Ferreira Machado, Cláudio Galuppo Diniz, Vania Lucia Da Silva, Vanessa Cordeiro Dias

{"title":"Unveiling antifungal resistance and biocide tolerance in clinical isolates of Candida spp.","authors":"Jamile De Paiva Macedo, Aripuana Aranha Sakurada Watanabe, Alessandra Barbosa Ferreira Machado, Cláudio Galuppo Diniz, Vania Lucia Da Silva, Vanessa Cordeiro Dias","doi":"10.1080/17460913.2025.2480924","DOIUrl":"https://doi.org/10.1080/17460913.2025.2480924","url":null,"abstract":"Aims: Candidiasis, caused by Candida spp. is an opportunistic infection with significant healthcare risks, worsened by trends in antifungal resistance. This study aimed to evaluate the antifungal susceptibility profile, investigate resistance mechanisms, assess efflux pump activity, and examine biocide tolerance in clinical Candida isolates.Methods: A total of 100 Candida isolates from hospitalized and outpatient individuals were analyzed for their antifungal susceptibility profile, molecular resistance mechanisms through PCR, efflux pump activity with the Cartwheel method, and biocide tolerance (sodium hypochlorite, hydrogen peroxide, and benzalkonium chloride), which was assessed by disk diffusion.Results: A high prevalence of resistance (87%) to at least one antifungal was observed, with 47.12% of isolates showing simultaneous multiple resistance to three azole derivatives. The highest antifungal agent resistance was observed for fluconazole (n = 70) and the highest susceptibility for amphotericin B (n = 1). The most common mutation was in the ERG11 gene (n = 38/43.7%). Efflux pump activity was detected in both C. albicans and non-albicans Candida species. Biocide testing revealed a higher tolerance for sodium hypochlorite, with an inhibition zone ranging from 18.25 (4.40) to 34.0 (4.00).Conclusions: This study highlights significant antifungal resistance in Candida spp. particularly to azoles, stressing the need for improved infection control and novel therapeutic strategies.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-12"},"PeriodicalIF":2.5,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143676911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface display of LukF-PV on Bacillus subtilis spore for oral administration.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-21 DOI: 10.1080/17460913.2025.2480933

Nhi Ny Nguyen, Lan Duong, Minh B Doan, Hoang Duc Nguyen

{"title":"Surface display of LukF-PV on Bacillus subtilis spore for oral administration.","authors":"Nhi Ny Nguyen, Lan Duong, Minh B Doan, Hoang Duc Nguyen","doi":"10.1080/17460913.2025.2480933","DOIUrl":"10.1080/17460913.2025.2480933","url":null,"abstract":"Aim: Bacillus subtilis spores are promising candidates for oral vaccine, serving as carriers for foreign proteins from pathogens. Here we utilized CotB, a spore coat protein, to anchor the LukF-PV from Staphylococcus aureus onto the surface of B. subtilis spores. Our goal was to generate a new B. subtilis strain and assess the capacity of the recombinant spore strain to induce antibody production in mice.Methods: The fusion sequence of cotB-lukF-PV was cloned into E. coli and transformed into B. subtilis HT800F. Colony PCR confirmed the generation of the recombinant strain. SporeELISA verified the display of LukF-PV. Mice were orally gavaged with the spore, and the production of IgA antibodies in feces and IgG in blood was evaluated.Results: We generated a new B. subtilis strain, BsHT2332, that integrated the PcotB-cotB-lukF-PV into its genome at amyE locus. BsHT2332 successfully displayed LukF-PV on the spore surface. The recombinant spores induced significant IgA and IgG immune responses in mice.Conclusion: This study demonstrated that B. subtilis spores expressing the S. aureus antigen LukF-PV can induce an immune response. These findings underscore the potential of the B. subtilis spore platform as a promising approach for vaccine development against S. aureus.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-9"},"PeriodicalIF":2.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of melanin in Fonsecaea monophora pathogenicity: adhesion, fibroblast interactions, and implications for chromoblastomycosis.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-20 DOI: 10.1080/17460913.2025.2480973

Tengteng Xin, Yushi Wu, Junmin Zhang

{"title":"The role of melanin in Fonsecaea monophora pathogenicity: adhesion, fibroblast interactions, and implications for chromoblastomycosis.","authors":"Tengteng Xin, Yushi Wu, Junmin Zhang","doi":"10.1080/17460913.2025.2480973","DOIUrl":"https://doi.org/10.1080/17460913.2025.2480973","url":null,"abstract":"Aims: Fonsecaea monophora is a pathogen of chromoblastomycosis (CBM), and melanin is one of the important virulence factors of F.monophora. Despite the crucial role fibroblasts play in inflammatory diseases, the interaction between fibroblasts and F.monophora remains poorly understood. This study investigated fibroblast responses to wild-type (WT) and polyketide synthase A gene knockout (ΔpksA) strains, aiming to elucidate the role of fungal melanin in adhesion mechanisms and the interactions between fibroblasts and pathogens.Materials & methods: Coculture systems of fibroblasts with WT and ΔpksA strains of F.monophora were established. Adhesion and ectophosphatase activity assays evaluated fungal attachment. Cell scratch and Transwell migration assays assessed fibroblast motility. RT-qPCR and ELISA quantified collagen synthesis, myofibroblast differentiation, and apoptosis-related gene expression.Results and conclusions: The WT strain exhibited greater adhesion and inhibited fibroblast migration, whereas the ΔpksA strain showed reduced adhesion and enhanced migration, correlating with melanin content and ectophosphatase activity. Both strains inhibited fibroblast-to-myofibroblast differentiation, collagen synthesis, and extracellular matrix deposition, contributing to tissue remodeling. These findings highlight melanin's role in F. monophora's pathogenicity and its disruption of fibroblast function, potentially delaying wound healing in CBM.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-8"},"PeriodicalIF":2.5,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A family cluster of Brucella abortus infections possibly due to contact with a sika deer in Northeast China.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-19 DOI: 10.1080/17460913.2025.2479297

Kun Li, Dawei Cheng, Zhifeng Xing, Yu Fan, Qingqing Xu, Xueying Chen, Meinan Li, Hongyan Zhao, Dongri Piao, Hai Jiang

引用次数: 0

Reviving the past for a healthier future: ancient molecules and remedies as a solution to the antibiotic crisis.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-18 DOI: 10.1080/17460913.2025.2476290

Miguel Ángel Díaz-Guerrero, Israel Castillo-Juárez, Rimma Zurabian, Alejandra Valdez, Kokila Kota, Yuki Hoshiko, Ekaprana Ramesh, Mariano Martínez-Vazquez, Corina Diana Ceapă, Mariel Hernandez-Garnica, Frederic Cadet, Rodolfo García-Contreras

{"title":"Reviving the past for a healthier future: ancient molecules and remedies as a solution to the antibiotic crisis.","authors":"Miguel Ángel Díaz-Guerrero, Israel Castillo-Juárez, Rimma Zurabian, Alejandra Valdez, Kokila Kota, Yuki Hoshiko, Ekaprana Ramesh, Mariano Martínez-Vazquez, Corina Diana Ceapă, Mariel Hernandez-Garnica, Frederic Cadet, Rodolfo García-Contreras","doi":"10.1080/17460913.2025.2476290","DOIUrl":"10.1080/17460913.2025.2476290","url":null,"abstract":"Options to combat bacterial infections are becoming scarce. We require innovative approaches to enhance the discovery of effective antimicrobials capable of combating bacteria resistant to multiple or all antibiotics. These methods should either directly eliminate resistant bacteria or indirectly influence their viability by inhibiting their virulence or reducing their resistance to antibiotics. One interesting approach is to analyze ancient remedies used to treat bacterial infections, formulate them, and test them against modern microbes. This field has recently been named \"ancientbiotics.\" This approach allows us to leverage centuries of empirical knowledge accumulated, from traditional medicines across various ancient cultures worldwide. The strategy has already yielded promising formulations to combat the ESKAPE group of nosocomial pathogens. Additionally, molecular de-extinction, which involves genome analysis of extinct species to search for useful antimicrobials, such as peptides, offers another avenue. In this review, we compile the antimicrobial effects of ancient remedies and de-extinct molecules known to modern science and discuss possible new strategies to further harness the potential of past remedies and molecules to fight the rise of superbugs.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-13"},"PeriodicalIF":2.5,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Not all disinfectants are created equal: the importance of mechanistic understanding to drive research forward. 并非所有消毒剂都是一样的：从机制上理解推动研究向前发展的重要性。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-17 DOI: 10.1080/17460913.2025.2480946

Elise L Bezold, Kevin P C Minbiole, William M Wuest

引用次数: 0

Clinical Spectrum of Toxocariasis: A Retrospective Study From a Tertiary Care Center in Lebanon.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-13 DOI: 10.1080/17460913.2025.2477919

Nour El Meski, L'Emir Wassim El Ayoubi, Samar Hassani, Nayda Bidikian, Johnny Zakhour, Jad Bou Khalil, Zeina A Kanafani, Souha S Kanj

{"title":"Clinical Spectrum of Toxocariasis: A Retrospective Study From a Tertiary Care Center in Lebanon.","authors":"Nour El Meski, L'Emir Wassim El Ayoubi, Samar Hassani, Nayda Bidikian, Johnny Zakhour, Jad Bou Khalil, Zeina A Kanafani, Souha S Kanj","doi":"10.1080/17460913.2025.2477919","DOIUrl":"https://doi.org/10.1080/17460913.2025.2477919","url":null,"abstract":"Aims: Toxocariasis is a prevalent zoonotic disease worldwide caused primarily by Toxocara canis and Toxocara cati. Despite its prevalence, studies focusing on clinical manifestations, and laboratory findings of toxocariasis in the Middle East North African (MENA) region are limited, highlighting a significant gap in research. The aim of this study is to describe the spectrum of toxocariasis observed at a tertiary center in Lebanon and review the geographic distribution of infected individuals and their demographics.Methods: The medical records of 225 patients who tested positive for Toxocara IgG via ELISA between 2002 and 2022 at the American University of Beirut Medical Center (AUBMC) were reviewed.Results: 141/225 patients had confirmed positive Toxocara-specific IgG by Western blot. These patients were mostly middle-aged, with a mean of 46 years and predominantly males. Most were Lebanese (95.3%), residing in Mount Lebanon or Beirut governates. Patients were mainly symptomatic (78.5%), and the most common presenting symptoms were neurological complaints. For those with Toxocara myelitis (23 patients), spine magnetic resonance imaging (MRI) findings showed focal lesions (54.5%) mostly at the thoracic vertebrae.Conclusions: Given the challenges associated with diagnosing this disease, further studies are essential to enhance our understanding of toxocariasis in the region.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-9"},"PeriodicalIF":2.5,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0