Future microbiology最新文献

Sublingual MV140 vaccine: a promising noninvasive approach for recurrent urinary tract infections in women. 舌下MV140疫苗：一种治疗女性复发性尿路感染的有前途的无创方法

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-05-13 DOI: 10.1080/17460913.2025.2503695

John Carlo Pansaon Piedad, Bob Yang

{"title":"Sublingual MV140 vaccine: a promising noninvasive approach for recurrent urinary tract infections in women.","authors":"John Carlo Pansaon Piedad, Bob Yang","doi":"10.1080/17460913.2025.2503695","DOIUrl":"https://doi.org/10.1080/17460913.2025.2503695","url":null,"abstract":"Recurrent urinary tract infections (rUTIs) are a growing global public health problem that has a significant impact on patient morbidity, quality of life, and economic burden. This is compounded by excessive antibiotic use in rUTI prophylaxis, prompting consideration of non-antibiotic prophylactic approaches. There has been increasing understanding of the pathophysiology of rUTIs, where bacteria subvert the host immune response and evade therapeutic strategies. Immuno-prophylaxis with monovalent and polyvalent bacterial preparations to elicit an immune response have come into the spotlight. Increasing understanding of how they achieve this based on innovative mechanistic in vitro and in vivo studies has highlighted the role of enhancing the helper T cell type 1 and diminishing the type 2 response. MV140 (Inmunotek SL, Madrid, Spain) is a quadrivalent bacterial preparation which has contemporary evidence to show good efficacy in improving rUTI clinical parameters, which is acceptable to patients, associated with improvements in quality of life, as well as reduced healthcare resource usage and costs. Importantly, it also appears to be safe and is associated with improved rationalization of antibiotic use. Whilst more rigorous studies are awaited, it shows promise in tackling heterogenous clinical entity.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-9"},"PeriodicalIF":2.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiviral defense against filovirus infections: targets and evasion mechanisms. 丝状病毒感染的抗病毒防御：靶点和逃避机制。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-05-07 DOI: 10.1080/17460913.2025.2501924

Bianca S Bodmer, Lisa Wendt, Juliette Dupré, Allison Groseth, Thomas Hoenen

{"title":"Antiviral defense against filovirus infections: targets and evasion mechanisms.","authors":"Bianca S Bodmer, Lisa Wendt, Juliette Dupré, Allison Groseth, Thomas Hoenen","doi":"10.1080/17460913.2025.2501924","DOIUrl":"https://doi.org/10.1080/17460913.2025.2501924","url":null,"abstract":"Filoviruses include a number of serious human pathogens, infections with which result in the development of hemorrhagic fevers with high case fatality rates. As for other RNA viruses, viral replication generates both protein and RNA species that can serve as danger signals, leading to the activation of antiviral defense pathways. However, in order to be able to efficiently infect humans these viruses have developed mechanisms that allow them to evade diverse host antiviral defense mechanisms. Consequently, in addition to their functions within the viral lifecycle many filovirus proteins have been shown to have accessory functions involved in the regulation of diverse host pathways. These include those of the type-I interferon response, other pathways involved in dsRNA-sensing, as well as the selective inhibition of interferon stimulated gene activities. Further, filoviruses have developed mechanisms to subvert recognition of infected cells and the generation of neutralizing antibodies. This review focuses on bringing together the evidence to date supporting the existence of diverse mechanisms aimed at regulating these pathways as well as providing details of the mechanisms involved.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-15"},"PeriodicalIF":2.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selected honey as a multifaceted antimicrobial agent: review of compounds, mechanisms, and research challenges. 选择蜂蜜作为一个多方面的抗菌剂：化合物，机制和研究挑战的回顾。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-28 DOI: 10.1080/17460913.2025.2498233

António Machado, Duarte Toubarro, José Baptista, Eduardo Tejera, José M Álvarez-Suárez

{"title":"Selected honey as a multifaceted antimicrobial agent: review of compounds, mechanisms, and research challenges.","authors":"António Machado, Duarte Toubarro, José Baptista, Eduardo Tejera, José M Álvarez-Suárez","doi":"10.1080/17460913.2025.2498233","DOIUrl":"https://doi.org/10.1080/17460913.2025.2498233","url":null,"abstract":"Honey, derived from floral nectar, has been valued for its nutritional and therapeutic properties, with recent studies emphasizing its broad-spectrum antimicrobial potential, especially against antimicrobial resistance (AMR). Honey's antimicrobial activity stems from its unique composition, including high sugar content, low pH, and bioactive compounds like hydrogen peroxide, methylglyoxal (MGO), and phenolic compounds. Distinct honey types, such as Manuka, Sidr, and Tualang, demonstrate varying antimicrobial effects based on their botanical and geographical origins. Manuka honey, rich in MGO, is notably effective against multidrug-resistant pathogens, while Sidr and heather honeys excel in biofilm inhibition and antioxidative properties. Bioactive components, including phenolics, flavonoids, enzymes, and antimicrobial peptides, disrupt microbial membranes, inhibit metabolic pathways, and induce oxidative stress. Advanced analytical techniques like HPLC and GC-MS have identified these compounds, though gaps remain in understanding secondary metabolites and synergistic actions. This review highlights honey's potential as a sustainable antimicrobial resource, emphasizing the need for standardization, clinical validation, and interdisciplinary research. Honey represents a promising solution to AMR and offers opportunities for integration into modern medicine and healthcare strategies.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-22"},"PeriodicalIF":2.5,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial action and mechanism of piscidin against Helicobacter pylori. 鱼青素对幽门螺杆菌的抑菌作用及机制研究。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-22 DOI: 10.1080/17460913.2025.2491253

Yunhai Wei, Wenjing Hu, Huancen Guo, Jinyu Zhang, Haiyan Dong, Chu Shen, Jingrui Cao, Zhongxin Wu, Lei Yin, Yuhai Gao

{"title":"Antimicrobial action and mechanism of piscidin against Helicobacter pylori.","authors":"Yunhai Wei, Wenjing Hu, Huancen Guo, Jinyu Zhang, Haiyan Dong, Chu Shen, Jingrui Cao, Zhongxin Wu, Lei Yin, Yuhai Gao","doi":"10.1080/17460913.2025.2491253","DOIUrl":"https://doi.org/10.1080/17460913.2025.2491253","url":null,"abstract":"Background: The rise of antibiotic-resistant Helicobacter pylori (Hp) strains is complicating the management of common gastrointestinal conditions such as gastritis and ulcers. In search of effective treatment options, antimicrobial peptides are being explored as a promising strategy.Methods: Leveraging bioinformatics, we dissected structural and sequence profiles of piscidin, an antimicrobial peptide. We investigated whether long-term piscidin exposure induces resistance in Hp strains by measuring their minimum inhibitory concentration. The Oxford cup test was applied to measure inhibition zones of piscidin against Hp strains. Following piscidin treatment, we monitored morphological alterations in Hp strains using scanning electron microscopy, and changes in urease activity, expressions of virulence factors CagA and UreB, and adenosine triphosphate levels. Cytotoxicity of piscidin was evaluated in AGS and 293T cells.Results: Piscidin inhibited proliferation of Hp strains, with a low propensity of inducing resistance. Delving into underlying mechanisms, we observed piscidin could alter morphology of Hp strains, suppress urease activity, and downregulate expressions of virulence factors CagA and UreB, while also reducing intracellular ATP levels. Piscidin had good security.Conclusion: Piscidin is a potent antibacterial agent against Hp with low resistance risk, making it a safe and effective treatment candidate.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-10"},"PeriodicalIF":2.5,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering probiotic potential: a comprehensive guide to probiogenomic analyses. 解读益生菌潜力：益生菌基因组分析的综合指南。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-14 DOI: 10.1080/17460913.2025.2492472

Yasmin Neves Vieira Sabino, Aline Dias Paiva, Bárbara Ribeiro Fonseca, Julliane Dutra Medeiros, Alessandra Barbosa Ferreira Machado

{"title":"Deciphering probiotic potential: a comprehensive guide to probiogenomic analyses.","authors":"Yasmin Neves Vieira Sabino, Aline Dias Paiva, Bárbara Ribeiro Fonseca, Julliane Dutra Medeiros, Alessandra Barbosa Ferreira Machado","doi":"10.1080/17460913.2025.2492472","DOIUrl":"https://doi.org/10.1080/17460913.2025.2492472","url":null,"abstract":"In recent years, the study of probiotics has advanced significantly, driven by growing interest in their potential health benefits and applications in the food and pharmaceutical industries. Probiotics are claimed to enhance gut health, modulate immune responses, improve digestion, synthesize beneficial compounds for the host, and even impact mental health through the gut-brain axis. However, traditional in vitro methods for identifying probiotics have limitations, such as low reproducibility in phenotypic screening, limited capacity to discover new strains, restricted evaluation of safety, and inefficiencies in fully understanding the biological properties responsible for health-promoting effects. Advancements in genomic analysis technology have provided a cost-effective approach to further explore probiotic strains and enhance understanding of the molecular mechanisms driving their beneficial effects in hosts. Here, we describe a comprehensive workflow for probiogenomic analysis aimed at establishing a gold-standard pipeline for screening probiotic potential based on genome sequencing. This pipeline encompasses steps from acquiring genomes to screening for safety-related features, genomic plasticity, and probiotic markers through whole-genome sequencing. In addition, this study outlines the respective methodological approaches and provides the most comprehensive database documented to date, comprising 243 genes potentially associated with probiotic function.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-12"},"PeriodicalIF":2.5,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing colistin efficacy with combination therapies for multidrug-resistant P. aeruginosa and A. baumannii isolates. 联合治疗多药耐药铜绿假单胞菌和鲍曼假单胞菌提高粘菌素疗效。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-10 DOI: 10.1080/17460913.2025.2490377

Elif Karaaslan, Ozlem Oyardi, Sibel Dosler

{"title":"Enhancing colistin efficacy with combination therapies for multidrug-resistant P. aeruginosa and A. baumannii isolates.","authors":"Elif Karaaslan, Ozlem Oyardi, Sibel Dosler","doi":"10.1080/17460913.2025.2490377","DOIUrl":"https://doi.org/10.1080/17460913.2025.2490377","url":null,"abstract":"Aim: Increasing resistance among ESKAPEEc pathogens, particularly Acinetobacter baumannii and Pseudomonas aeruginosa, has necessitated the use of last-resort antibiotics such as colistin. This study aimed to evaluate the effectiveness and reveal a dynamic picture of colistin-based combination therapies.Materials & methods: This study evaluated the in vitro efficacy of colistin in combination with doxycycline, doripenem, and rifampicin against multidrug-resistant clinical isolates of P. aeruginosa (n = 23) and A. baumannii (n = 26). Susceptibility testing performed by microbroth dilution method, and synergistic interactions were assessed via checkerboard and time-kill curve (TKC) assays.Results: All isolates were resistant to colistin, according to their MICs. In checkerboard assays, according to synergism rates, colistin-doripenem and colistin-doxycycline combinations were particularly effective. The degrees of synergy for doripenem, doxycycline, and rifampicin were 30%, 90%, and 20%, respectively, against P. aeruginosa, and 30%, 60%, and 30%, respectively, against A. baumannii. In TKC analysis, synergistic interactions are generally observed with colistin at 1/4×MIC or 1×MIC, and indifference effects at 4×MIC, similar to colistin monotherapy. TKCs also confirmed the bactericidal activities of combinations that achieved ≥3-log10 reductions in initial bacterial counts.Conclusions: Colistin-based combination therapies, especially colistin-doripenem, may be promising approaches for combating multidrug-resistant pathogens while potentially reducing nephrotoxicity risk.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-9"},"PeriodicalIF":2.5,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional efficacy of Enterococcus faecium HN4 and Lactobacillus delbrueckii HN5 strains isolated from human milk. 人乳中分离的粪肠球菌HN4和德布吕氏乳杆菌HN5的功能效果。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-28 DOI: 10.1080/17460913.2025.2484924

Harsha Nirvan, Garima Deswal, Manjit K Selwal, Krishan Kumar Selwal

引用次数: 0

Resistance genes are not like chemical pollutants and surveillance of them should reflect that. 抗性基因不像化学污染物，对它们的监测应该反映这一点。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-10 DOI: 10.1080/17460913.2025.2476880

Patrick Munk, Christian Brinch, Frank M Aarestrup

引用次数: 0

Sodium ibuprofenate: antibacterial activities and potential β-lactamase inhibition in critical Gram-negative bacteria. 布洛芬酸钠：抗菌活性和潜在的β-内酰胺酶抑制临界革兰氏阴性菌。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-09 DOI: 10.1080/17460913.2025.2475639

Franco A Manzanelli, Camila M Clemente, Luciana P Campagno, Dante M Beltramo, Sara M Robledo, Soledad Ravetti, Ariel G Garro

{"title":"Sodium ibuprofenate: antibacterial activities and potential β-lactamase inhibition in critical Gram-negative bacteria.","authors":"Franco A Manzanelli, Camila M Clemente, Luciana P Campagno, Dante M Beltramo, Sara M Robledo, Soledad Ravetti, Ariel G Garro","doi":"10.1080/17460913.2025.2475639","DOIUrl":"10.1080/17460913.2025.2475639","url":null,"abstract":"Aims: To evaluate the antibacterial and antibiofilm activities of sodium ibuprofenate (NaI) and its hypertonic variant (NaIHS) against multidrug-resistant Gram-negative bacteria (MDR-GNB) and explore their potential to inhibit β-lactamase enzymes.Methods: Antibacterial activity was assessed using minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and time-kill assays. Antibiofilm activity was evaluated by measuring bacterial viability and biomass reduction in preformed biofilms. Scanning electron microscopy (SEM) was used to observe membrane effects. Molecular docking and molecular dynamics simulations were conducted to analyze the binding affinity of ibuprofen to the active sites of β-lactamases (CTX-M-15, KPC-2, OXA-23).Results: NaI exhibited bactericidal activity at concentrations of 25-75 mm, with Acinetobacter baumannii being the most susceptible. NaCl (≥0.5 M) enhanced bactericidal efficacy and lowered MBCs. Time-kill assays indicated rapid bacterial eradication within 2 hours, with NaIHS achieving similar results at lower concentrations. SEM confirmed membrane disruption. Both formulations reduced bacterial viability in biofilms, with NaIHS showing greater efficiency. In silico studies suggest ibuprofen may inhibit β-lactamases, with enhanced interactions in saline environments.Conclusion: Sodium ibuprofenate, particularly in its hypertonic form, demonstrates strong antibacterial, antibiofilm, and potential β-lactamase inhibitory activity, making it a promising candidate for treating MDR-GNB infections.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"395-407"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reviving the past for a healthier future: ancient molecules and remedies as a solution to the antibiotic crisis. 为更健康的未来复兴过去：古老的分子和药物作为抗生素危机的解决方案。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-18 DOI: 10.1080/17460913.2025.2476290

Miguel Ángel Díaz-Guerrero, Israel Castillo-Juárez, Rimma Zurabian, Alejandra Valdez, Kokila Kota, Yuki Hoshiko, Ekaprana Ramesh, Mariano Martínez-Vazquez, Corina Diana Ceapă, Mariel Hernandez-Garnica, Frederic Cadet, Rodolfo García-Contreras

{"title":"Reviving the past for a healthier future: ancient molecules and remedies as a solution to the antibiotic crisis.","authors":"Miguel Ángel Díaz-Guerrero, Israel Castillo-Juárez, Rimma Zurabian, Alejandra Valdez, Kokila Kota, Yuki Hoshiko, Ekaprana Ramesh, Mariano Martínez-Vazquez, Corina Diana Ceapă, Mariel Hernandez-Garnica, Frederic Cadet, Rodolfo García-Contreras","doi":"10.1080/17460913.2025.2476290","DOIUrl":"10.1080/17460913.2025.2476290","url":null,"abstract":"Options to combat bacterial infections are becoming scarce. We require innovative approaches to enhance the discovery of effective antimicrobials capable of combating bacteria resistant to multiple or all antibiotics. These methods should either directly eliminate resistant bacteria or indirectly influence their viability by inhibiting their virulence or reducing their resistance to antibiotics. One interesting approach is to analyze ancient remedies used to treat bacterial infections, formulate them, and test them against modern microbes. This field has recently been named \"ancientbiotics.\" This approach allows us to leverage centuries of empirical knowledge accumulated, from traditional medicines across various ancient cultures worldwide. The strategy has already yielded promising formulations to combat the ESKAPE group of nosocomial pathogens. Additionally, molecular de-extinction, which involves genome analysis of extinct species to search for useful antimicrobials, such as peptides, offers another avenue. In this review, we compile the antimicrobial effects of ancient remedies and de-extinct molecules known to modern science and discuss possible new strategies to further harness the potential of past remedies and molecules to fight the rise of superbugs.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"429-441"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0