Future microbiology最新文献

Arginine combination with fluoride and calcium glycerophosphate: effects of concentration and on biofilm fluid. 精氨酸与氟化物和甘油磷酸钙的结合：浓度和对生物膜液体的影响。

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-11-07 DOI: 10.1080/17460913.2024.2411921

Marina Lins Miranda, Karina Borges Salomão, Alberto Carlos Botazzo Delbem, Marcelle Danelon, Elis Rodrigues Oliveira Barbosa, Caio Sampaio, Lucas Arrais Campos, Fernanda Lourenção Brighenti

{"title":"Arginine combination with fluoride and calcium glycerophosphate: effects of concentration and on biofilm fluid.","authors":"Marina Lins Miranda, Karina Borges Salomão, Alberto Carlos Botazzo Delbem, Marcelle Danelon, Elis Rodrigues Oliveira Barbosa, Caio Sampaio, Lucas Arrais Campos, Fernanda Lourenção Brighenti","doi":"10.1080/17460913.2024.2411921","DOIUrl":"https://doi.org/10.1080/17460913.2024.2411921","url":null,"abstract":"Aim: To study the influence of varying concentrations of arginine (Arg) combined with fluoride (F) and/or calcium glycerophosphate (CaGP) on biofilms.Materials & methods: Biofilms were analyzed for acidogenicity, microbial viability and Ca, F and inorganic phosphorus (P) concentrations.Results: For total bacteria, the lowest viability was found in F-containing groups, regardless of the arginine concentrations and presence of CaGP. For aciduric bacteria, no significant differences were found among arginine concentrations in the presence of F. For MS, arginine concentrations did not influence MS viability in the presence of fluoride and CaGP only decreased viability at 3.2% Arg concentration. The arginine-treated groups showed the lowest acidogenicity. For ion concentrations in biofilms, CaGP showed the highest values for P; Arg+F for F; and CaGP/Arg+CaGP for Ca.Conclusion: Different concentrations of arginine did not affect the microbial viability or acidogenicity of biofilms. Moreover, 0.8% Arg did not increase ion concentration in biofilm fluid.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural polymicrobial Staphylococcus aureus-Pseudomonas aeruginosa interactions and antimicrobial resistance in ex vivo cornea model. 体外角膜模型中金葡萄球菌-铜绿假单胞菌相互作用的超微结构和抗菌药耐药性。

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-11-06 DOI: 10.1080/17460913.2024.2417617

Sanchita Mitra, Nagapriya Banka, Soumyava Basu, Tirupathi Rao

{"title":"Ultrastructural polymicrobial Staphylococcus aureus-Pseudomonas aeruginosa interactions and antimicrobial resistance in ex vivo cornea model.","authors":"Sanchita Mitra, Nagapriya Banka, Soumyava Basu, Tirupathi Rao","doi":"10.1080/17460913.2024.2417617","DOIUrl":"https://doi.org/10.1080/17460913.2024.2417617","url":null,"abstract":"Aim: To investigate antagonistic interactions among pathogens, in ex vivo donor corneas infected with monomicrobial or polymicrobial combinations of antibiotic susceptible and resistant clinical isolates of Staphylococcus aureus (MSSA, MRSA) and Pseudomonas aeruginosa (S-PA, MDR-PA).Materials & methods: Scanning electron microscopy and antimicrobial susceptibility testing (AST, broth microdilution for minimum inhibitory and bactericidal concentrations [MIC/MBC]) pre-and post-polymicrobial interactions, in infected donor corneas.Results: MSSA lost viability with S-PA/MDR-PA, while MRSA formed larger cells, biofilm and lower MIC (teicoplanin) with S-PA, but lost viability with MDR-PA. S-PA had lower MIC (ceftazidime, meropenem, chloramphenicol) with MSSA, and lower MBC (cefoperazone, ciprofloxacin) and fewer cells with MRSA. MDR-PA had abundant cells and no change in AST with MSSA or MRSA.Conclusion: Significant antagonistic interactions occur in ocular polymicrobial infections, affecting antibiotic susceptible isolates more than resistant ones.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142580806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosensing technology for detection and assessment of pathogenic microorganisms. 用于检测和评估病原微生物的生物传感技术。

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-10-29 DOI: 10.1080/17460913.2024.2417621

Sakshi Sinha, Lata Sheo Bachan Upadhyay

{"title":"Biosensing technology for detection and assessment of pathogenic microorganisms.","authors":"Sakshi Sinha, Lata Sheo Bachan Upadhyay","doi":"10.1080/17460913.2024.2417621","DOIUrl":"10.1080/17460913.2024.2417621","url":null,"abstract":"At present, the prevalence of infectious diseases is rising annually, making it an important risk factor for human health that should not be neglected. Consequently, infection control and prevention have become even more important. The key to determining and designing the most effective anti-infectious medication depends upon the immediate and accurate identification of the causative agent. The standard techniques used for routine infection screening and surveillance tests are shifting toward biosensors. Furthermore, biosensors are projected to be employed for microbiological detection to satisfy the higher accuracy required for clinical diagnosis. This is because of their compact size, real-time monitoring and ability to analyze large sample numbers with less sophistication and manpower requirement, which have allowed them to develop quickly with extensive uses. Biosensors have multiple applications in food safety, environmental surveillance, drug sensing and national security because they offer several advantages such as quick response, outstanding sensitivity, remarkable selectivity, high degree of accuracy and precision, ease of use and affordable price. This review highlights the performance aspects of recently developed biosensors for the detection of infectious bacteria and viruses in biological and environmental samples and emphasizes the significance of nanotechnology in signal amplification for enhanced biosensor performance and dependability.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 更正。

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-10-29 DOI: 10.1080/17460913.2024.2422226

引用次数: 0

Microbiome and bladder cancer: the role of probiotics in treatment. 微生物组与膀胱癌：益生菌在治疗中的作用。

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-10-24 DOI: 10.1080/17460913.2024.2414671

Leila Dadgar-Zankbar, Maryam Mokhtaryan, Elnaz Bafandeh, Zahra Javanmard, Parisa Asadollahi, Taleih Darbandi, Roghayeh Afifirad, Shirin Dashtbin, Atieh Darbandi, Roya Ghanavati

{"title":"Microbiome and bladder cancer: the role of probiotics in treatment.","authors":"Leila Dadgar-Zankbar, Maryam Mokhtaryan, Elnaz Bafandeh, Zahra Javanmard, Parisa Asadollahi, Taleih Darbandi, Roghayeh Afifirad, Shirin Dashtbin, Atieh Darbandi, Roya Ghanavati","doi":"10.1080/17460913.2024.2414671","DOIUrl":"https://doi.org/10.1080/17460913.2024.2414671","url":null,"abstract":"Bladder cancer (BCa) remains a significant global health challenge, with increasing interest in the role of the bladder microbiome in its pathogenesis, progression and treatment outcomes. The complex relationship between bladder cancer and the microbiome, as well as the potential impact of probiotics on treatment effectiveness, is currently under investigation. Research suggests that the microbiota may influence BCa recurrence prevention and enhance the efficacy of the Bacillus Calmette-Guérin (BCG) vaccine. Recent studies reveal differences in the bladder microbiome between individuals without bladder cancer and those with the disease. In the healthy bladder, Streptococcus and Lactobacillus are consistently identified as the most prevalent genera. However, in men, the predominant bacterial genera are Staphylococcus, Corynebacterium and Streptococcus, while in women with bladder cancer, Gardnerella and Lactobacillus are dominant. Probiotics, particularly Lactobacillus spp., can exhibit anti-tumor properties by competing with pathogenic strains involved in carcinogenesis or by producing regulatory substances. They regulate cancer signaling, induce apoptosis, inhibit mutagenic activity, downregulate oncogene expression, induce autophagy, inhibit kinases, reactivate tumor suppressors and prevent metastasis. These mechanisms have shown promising results in both preclinical and some clinical studies.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new N-acylhydrazone oxadiazole derivative with activity against mycobacteria. 一种具有抗分枝杆菌活性的新型 N-酰腙噁二唑衍生物。

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-10-23 DOI: 10.1080/17460913.2024.2412439

Izabella Ventura Souza, Maria Luiza Fróes da Motta Dacome, Andrew Matheus Frederico Rozada, Jonathan Sanches Rosa, Eloisa Gibin Sampiron, Deisiany Gomes Ferreira, Gisele Freitas Gauze, Melyssa Fernanda Norman Negri, Regiane Bertin de Lima Scodro, Rosilene Fressatti Cardoso, Katiany Rizzieri Caleffi-Ferracioli

{"title":"A new N-acylhydrazone oxadiazole derivative with activity against mycobacteria.","authors":"Izabella Ventura Souza, Maria Luiza Fróes da Motta Dacome, Andrew Matheus Frederico Rozada, Jonathan Sanches Rosa, Eloisa Gibin Sampiron, Deisiany Gomes Ferreira, Gisele Freitas Gauze, Melyssa Fernanda Norman Negri, Regiane Bertin de Lima Scodro, Rosilene Fressatti Cardoso, Katiany Rizzieri Caleffi-Ferracioli","doi":"10.1080/17460913.2024.2412439","DOIUrl":"https://doi.org/10.1080/17460913.2024.2412439","url":null,"abstract":"Aim: To evaluate the anti-Mycobacterium tuberculosis (Mtb) potential of the hybrid oxadiazol-4-methoxynaphthalene (6n) derived from N-acylhydrazone (4k).Materials & methods: The study determined the minimal inhibitory concentration of (6n) against Mtb H37Rv and Mtb clinical isolates, potential combination of (6n) with anti-tuberculosis drugs and carried out time kill curve assay of Mtb H37Rv. Additional contribution for the analysis of (6n) was explored by in silico pharmacokinetics, and in vitro and in vivo cytotoxicity determinations.Results: The newly synthesized molecule (6n) demonstrated anti-Mtb activity, low cytotoxicity and selectivity for Mtb.Conclusion: The derivative (6n) emerges as a potential anti-TB drug candidate.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current trends to design antimalarial drugs targeting N-myristoyltransferase. 设计以 N-肉豆蔻酰转移酶为靶点的抗疟疾药物的当前趋势。

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-10-23 DOI: 10.1080/17460913.2024.2412397

Misael de Azevedo Teotônio Cavalcanti, Karla Joane Da Silva Menezes, Jéssika De Oliveira Viana, Éric de Oliveira Rios, Arthur Gabriel Corrêa de Farias, Karen Cacilda Weber, Fatima Nogueira, Igor José Dos Santos Nascimento, Ricardo Olimpio de Moura

引用次数: 0

Effect of amikacin-humic acid combination on Acinetobacter baumannii biofilm: an in vitro and in silico study. 阿米卡星-腐植酸复方制剂对鲍曼不动杆菌生物膜的影响：体外和硅学研究。

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-10-21 DOI: 10.1080/17460913.2024.2412431

Seetha Lakshmi Rajangam, Kakithakara Vajravelu Leela, Manonmoney Jayaraman, Sarvesh Sabarathinam, Manoj Kumar Narasimhan

{"title":"Effect of amikacin-humic acid combination on Acinetobacter baumannii biofilm: an in vitro and in silico study.","authors":"Seetha Lakshmi Rajangam, Kakithakara Vajravelu Leela, Manonmoney Jayaraman, Sarvesh Sabarathinam, Manoj Kumar Narasimhan","doi":"10.1080/17460913.2024.2412431","DOIUrl":"https://doi.org/10.1080/17460913.2024.2412431","url":null,"abstract":"Aim: Acinetobacter baumannii (AB) is a clinically important bacterial pathogen responsible for nosocomial infections. The biofilm-forming capability of these pathogens reduces the antibiotic penetration and its efficacy, thereby complicating the treatment. The current work aims to isolate the most potent biofilm-forming Acinetobacter species from clinical isolates of the patient samples and to evaluate the efficacy of the amikacin-humic acid combination against it.Methods: The combination effect of Amikacin-Humic (AMK-HUM) acid against the highest biofilm-producing A. baumannii SLMK001 was studied via in-vitro (microscopic analysis) and in-silico (Network Pharmacology) analysis.Results: The amikacin-humic acid combination significantly inhibited both the biofilm formation and cell viability of A. baumannii SLMK001. The images observed via Scanning Electron Microscope (SEM) showed a significant decrease in the biofilm matrix. Confocal Laser Scanning Microscope (CLSM) confirmed a reduction of the Z value of its three-dimensional structure. Further, the Network Pharmacology approach supported these experimental findings by identifying the key targets of the amikacin-humic acid combination against the biofilm pathways of A. baumannii.Conclusion: The in-vitro results aligned with the in-silico findings, indicating that the AMK-HUM combination is a promising treatment that significantly activates the key proteins against A. baumannii biofilm formation and pathogenesis.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142463284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering the role of VapBC toxin-antitoxin systems in Mycobacterium tuberculosis stress adaptation. 解密 VapBC 毒素-抗毒素系统在结核分枝杆菌应激适应中的作用。

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-10-21 DOI: 10.1080/17460913.2024.2412447

Zoozeal Thakur, Renu Chaudhary, Promod K Mehta

{"title":"Deciphering the role of VapBC toxin-antitoxin systems in Mycobacterium tuberculosis stress adaptation.","authors":"Zoozeal Thakur, Renu Chaudhary, Promod K Mehta","doi":"10.1080/17460913.2024.2412447","DOIUrl":"https://doi.org/10.1080/17460913.2024.2412447","url":null,"abstract":"Mycobacterium tuberculosis (Mtb) harbors a high number of Toxin-Antitoxin (TA) systems, wherein half of them belong to virulence associated proteins B and C (VapBC) family that has a characteristic PilT N-terminus domain and ribonuclease activity. Functional insights into Mtb VapBC TA modules unraveled their role in adaptation to various host-mediated stressors, including oxidative/nitrosative, chemical and nutrient starvation as well as multidrug tolerance and establishment of persistence. To understand the intricacies of Mtb's pathogenesis, absolute cellular targets of 19 VapC(s) were determined. Some exhibit a shared ribonuclease activity, whereas others harbor tRNAse and 23S rRNA cleavage activity. The detailed functional characterization of VapBC4, VapBC12 and VapBC22, including in vivo deletion mutant studies revealed their role in Mtb's virulence/persistence. For example, the VapC22 mutant was attenuated for Mtb's growth in mice and elicited a decreased TH1 response, whereas mice infected with VapC12 mutant displayed a substantially higher bacillary load and pro-inflammatory response than the wild type, showing a hyper-virulent phenotype. Further experimental studies are needed to decode the functional role of VapBC systems and unravel their cellular targets. Taken together, Mtb VapBC TA systems seem to be promising drug targets owing to their key role in enduring stressors, antibiotic resistance and persistence.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142463283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clioquinol influences cell membrane, attenuates virulence factors, induces apoptosis to inhibit Candida albicans growth. Clioquinol 可影响细胞膜、减弱毒力因子、诱导细胞凋亡，从而抑制白色念珠菌的生长。

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-10-17 DOI: 10.1080/17460913.2024.2408136

Zimeng You, Yaling Dai, Yuping Ran

{"title":"Clioquinol influences cell membrane, attenuates virulence factors, induces apoptosis to inhibit Candida albicans growth.","authors":"Zimeng You, Yaling Dai, Yuping Ran","doi":"10.1080/17460913.2024.2408136","DOIUrl":"https://doi.org/10.1080/17460913.2024.2408136","url":null,"abstract":"Aim: To investigate the antifungal mechanism of clioquinol and indicate that clioquinol has potential as a novel therapeutic antifungal agent.Materials & methods: Analyze differentially expressed genes of Candida albicans treated with clioquinol using RNA-sequencing. The effects on cell wall and membrane features, virulence factors, apoptosis-induced cell death were also investigated.Results: The differentially expressed genes of C. albicans after treated with clioquinol focused on cell wall and membrane synthesis, antioxidant system and energy metabolism. Clioquinol did not change cell wall components levels while it decreased squalene epoxidase activity to influence the ergosterol biosynthesis in cell membrane. It also decreased cellular surface hydrophobicity and induced β-glucan unmasking to attenuate virulence factors. Meanwhile, clioquinol influenced enzyme activities involved in antioxidant system, citrate cycle, oxidative phosphorylation and decreased the ATP levels. Clioquinol induced apoptosis in C. albicans to exert its fungicidal activity. It induced reactive oxygen species and calcium ion elevation, leading to loss of mitochondrial membrane potential, cytochrome C release, metacaspase activation, thereby triggering apoptosis.Conclusion: Clioquinol exerted anti-C. albicans activity through influencing cell membrane, attenuating virulence factors and inducing apoptosis.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142463282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0