Future microbiology最新文献

Aqueous leaf extract from Eugenia dysenterica exhibits antimicrobial and antibiofilm activity against Staphylococcus aureus. 赤霉素叶水提物对金黄色葡萄球菌具有抗菌和抗膜活性。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-07-24 DOI: 10.1080/17460913.2025.2528419

Ivanilde Carmo Dos Santos, Flávio Santos Lopes, Paulo Henrique Gonçalves Dias Diniz, Cláudia Vieira Prudêncio

{"title":"Aqueous leaf extract from Eugenia dysenterica exhibits antimicrobial and antibiofilm activity against Staphylococcus aureus.","authors":"Ivanilde Carmo Dos Santos, Flávio Santos Lopes, Paulo Henrique Gonçalves Dias Diniz, Cláudia Vieira Prudêncio","doi":"10.1080/17460913.2025.2528419","DOIUrl":"https://doi.org/10.1080/17460913.2025.2528419","url":null,"abstract":"Staphylococcus aureus is a pathogen associated with various diseases, exhibiting biofilm ability and antimicrobial resistance. The search for new antimicrobial agents, especially natural ones, is crucial. This study evaluated the antibacterial and antibiofilm activities of the aqueous leaf extract from Eugenia dysenterica against S. aureus. Healthy leaves were dried, powdered and infused in water to obtain the extract. Reference strains S. aureus ATCC 25,923 and ATCC 29,213 were employed as model organisms. The minimum inhibitory concentration (MIC) was assessed using the microdilution technique in 96-well polystyrene microplates. The minimum bactericidal concentration (MBC) was evaluated by plating on standard count agar. Test surface assays were performed with polystyrene coupons, where the count of viable sessile cells (CVSC) was determined after sonication and agitation. Biofilm resistance was assessed after exposure to a chlorinated compound. The extract showed antimicrobial activity, with MICs values of 156-313 μg/mL and MBC values of 625-1,250 μg/mL. Antibiofilm activity was observed at inhibitory and subinhibitory concentrations. Even when CVSC was similar, the extract increased biofilm sensitivity to chlorinated compounds. The antimicrobial and antibiofilm potential of the aqueous leaf extract of E. dysenterica could aid S. aureus control in various fields, including the food and medical industries.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-8"},"PeriodicalIF":2.5,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144707261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical characteristics and surgical outcomes of endogenous Klebsiella pneumoniae endophthalmitis. 内源性肺炎克雷伯菌眼内炎的临床特点及手术效果。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-07-12 DOI: 10.1080/17460913.2025.2530840

Jinshan Suo, Xuzhong Shen, Dan Li, Jing Jiang, Yiwen Qian, Xin Wang, Pei Zhang, Minggui Wang, Zhiliang Wang

{"title":"Clinical characteristics and surgical outcomes of endogenous Klebsiella pneumoniae endophthalmitis.","authors":"Jinshan Suo, Xuzhong Shen, Dan Li, Jing Jiang, Yiwen Qian, Xin Wang, Pei Zhang, Minggui Wang, Zhiliang Wang","doi":"10.1080/17460913.2025.2530840","DOIUrl":"https://doi.org/10.1080/17460913.2025.2530840","url":null,"abstract":"Purpose: To evaluate surgical outcomes and prognostic factors related to visual acuity (VA) in endogenous Klebsiella pneumoniae endophthalmitis (EKE).Materials and methods: This retrospective study included patients diagnosed with EKE who underwent pars plana vitrectomy (PPV). Patients were divided into two groups based on the best-corrected VA (BCVA). Eyes with BCVA worse than counting fingers (CF) were classified as having poor vision, while those with BCVA of CF or better were classified as having good vision. Prognostic factors for poor outcomes were identified using least absolute shrinkage and selection operator (LASSO) and logistic regression.Results: Overall, 64 eyes from 56 patients with EKE were included, of which 51 eyes (43 patients) underwent PPV. At final follow-up, BCVA had improved among patients with PPV. In this subgroup, the mean logarithm of the minimum angle of resolution (logMAR) VA improved from 2.269 ± 0.570 to 2.092 ± 0.713. The VA preservation rate was 86.667%. LASSO and logistic regression showed that three predictors of poor outcomes: poor initial VA, presence of corneal edema, and delayed PPV.Conclusions: Early PPV is associated with improved VA outcomes in EKE. Poor initial VA, corneal edema, and delayed surgery were significantly associated with worse visual prognosis.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-9"},"PeriodicalIF":2.5,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144617239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical characteristics of children with disseminated Staphylococcus aureus infection involving bone and joint. 儿童弥散性金黄色葡萄球菌感染累及骨和关节的临床特点。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-07-09 DOI: 10.1080/17460913.2025.2525709

Yingtie Cui, Yunzhen Zhang, Shiguang Feng, Zhen Mao, Pengyuan Luo, Xiaokang Zhou

{"title":"Clinical characteristics of children with disseminated Staphylococcus aureus infection involving bone and joint.","authors":"Yingtie Cui, Yunzhen Zhang, Shiguang Feng, Zhen Mao, Pengyuan Luo, Xiaokang Zhou","doi":"10.1080/17460913.2025.2525709","DOIUrl":"https://doi.org/10.1080/17460913.2025.2525709","url":null,"abstract":"This retrospective study investigated the clinical characteristics and prognosis in 24 children (average age 5.6 ± 4.9 years) with disseminated Staphylococcus aureus bone and joint infections (May 2011-November 2022). Most infections (91.67%) were found to be associated with respiratory system infections. The femur (16 cases) was the most common bone; hip (6 cases) and knee (5 cases) were the most common joints. Notably, 14 cases (58.33%) had infections at > 3 osteoarticular sites, and 6 cases (25.00%) at > 4 sites (maximum 7). Cultures revealed 58.33% methicillin-resistant S. aureus ;(MRSA) and 41.67% methicillin-sensitive S. aureus ;(MSSA). All patients underwent surgery; 6 required secondary procedures. Patients were divided into sequelae (n = 10) and non-sequelae (n = 14) groups. The sequelae group had significantly longer time to surgery (11.20 ± 8.46 vs. 3.50 ± 2.53 days, p = 0.019), higher procalcitonin (27.28 ± 23.98 ng/ml vs. 9.02 ± 9.00 ng/ml, p = 0.043), and greater bacterial load (2760.86 ± 1592.02 vs. 155.25 ± 65.57, p = 0.005). Disseminated S. aureus bone and joint infections in children commonly affect the femur, hip, and knee. Delayed surgery and higher PCT levels are associated with sequelae.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-8"},"PeriodicalIF":2.5,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of colistin resistance in clinical Klebsiella pneumoniae isolates: a systematic review and meta-analysis. 临床肺炎克雷伯菌分离株中粘菌素耐药性的流行：一项系统综述和荟萃分析。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-07-06 DOI: 10.1080/17460913.2025.2527534

Negar Narimisa, Shirin Bavari, Forough Goodarzi, Amin Khoshbayan

{"title":"Prevalence of colistin resistance in clinical Klebsiella pneumoniae isolates: a systematic review and meta-analysis.","authors":"Negar Narimisa, Shirin Bavari, Forough Goodarzi, Amin Khoshbayan","doi":"10.1080/17460913.2025.2527534","DOIUrl":"https://doi.org/10.1080/17460913.2025.2527534","url":null,"abstract":"Objective: Klebsiella pneumoniae commonly causes nosocomial infections, and the rise of colistin-resistant strains poses a significant challenge to public health. This research aims to assess the worldwide prevalence of colistin resistance in K. pneumoniae isolates through a systematic review of scientific literature.Methods: We conducted a systematic literature review of relevant studies available in the PubMed, Scopus, and Web of Science databases up to December 2023. Following this, a meta-analysis was performed using Stata software to determine the overall prevalence of colistin resistance in K. pneumoniae isolates and to conduct subgroup analyses.Results: This meta-analysis reviewed 682 studies and found a global prevalence of colistin resistance at 13% among all K. pneumoniae isolates. Notably, the data shows an increase in colistin resistance from 6% in 2010 to 18% in 2023.Conclusion: This study presents evidence of a substantial global prevalence of colistin resistance among clinical isolates of K. pneumoniae. It is recommended to conduct regular surveillance and testing for colistin resistance to effectively monitor and address this public health issue.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-10"},"PeriodicalIF":2.5,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond the blame game: fostering consumer engagement in addressing antimicrobial resistance. 超越指责游戏：促进消费者参与解决抗菌素耐药性问题。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-07-03 DOI: 10.1080/17460913.2025.2528405

Anna-Carin Nordvall, Elin Nilsson, Emma Oljans, Mirko Ancillotti

引用次数: 0

Next stop, precision: targeted therapies for Staphylococcus aureus infections. 下一站，精准：金黄色葡萄球菌感染的靶向治疗。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-07-03 DOI: 10.1080/17460913.2025.2528416

Frank J Hernandez

引用次数: 0

Evaluation of a bivalent vaccine composed of iron scavenger receptors PMI1426 and PMI1945 against Proteus mirabilis. 铁清除受体PMI1426和PMI1945组成的抗奇异变形杆菌二价疫苗的评价。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-07-02 DOI: 10.1080/17460913.2025.2525711

Roghayeh Mohammadzadeh, Mehri Habibi, Mohammad Reza Asadi Karam

{"title":"Evaluation of a bivalent vaccine composed of iron scavenger receptors PMI1426 and PMI1945 against Proteus mirabilis.","authors":"Roghayeh Mohammadzadeh, Mehri Habibi, Mohammad Reza Asadi Karam","doi":"10.1080/17460913.2025.2525711","DOIUrl":"https://doi.org/10.1080/17460913.2025.2525711","url":null,"abstract":"Aim: Urinary tract infections (UTIs) caused by Proteus mirabilis are common infectious diseases. Currently, no vaccine is available against UTIs, making the development of an effective vaccine a critical goal to reduce the prevalence and antibiotic resistance of UTIs. Hence, we have investigated the immunogenicity and protective potential of a bivalent vaccine candidate comprising the iron scavenger receptors PMI1426 and PMI1945 from P. mirabilis in mice.Methods: In this study, vaccine candidates composed of PMI1426 and PMI1945 from P. mirabilis were developed, and their immunogenicity, efficacy, and synergistic effects were evaluated in mice.Results: Serum levels of IgG, IgG isotypes, IgA, and urinary IgA, as well as cytokine levels including IFN-γ, IL-4, and IL-17 were significantly elevated following vaccination with PMI1426 and PMI1945 as compared to the controls. The addition of alum enhanced the immune responses in both single and combined vaccine formulations. Furthermore, bacterial colonization in the bladder of mice challenged with P. mirabilis was significantly reduced in mice received the proteins admixed with alum compared to control groups.Conclusion: Our results support the development of a promising vaccine candidate against P. mirabilis UTIs. Future human clinical trials are needed to validate the efficacy and safety of the vaccine candidates.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-11"},"PeriodicalIF":2.5,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Employing machine learning for identifying antifungal compounds against Candida albicans. 利用机器学习鉴定抗白色念珠菌的抗真菌化合物。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-07-02 DOI: 10.1080/17460913.2025.2525717

Dienny Rodrigues de Souza, Lívia Do Carmo Silva, Kleber Santiago Freitas E Silva, Fabricio Silva de Jesus, Amanda Alves de Oliveira, Bruno Junior Neves, Maristela Pereira

引用次数: 0

Microbial isolates and resistance profiles in cerebrospinal fluid cultures: a five-year experience at a tertiary center. 脑脊液培养中的微生物分离物和耐药性：三级中心的五年经验。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-07-01 Epub Date: 2025-06-18 DOI: 10.1080/17460913.2025.2520666

Merve Gürler, Füsun Kırca, Figen Türen, İrem Tümkaya Kılınç, Ferhat Gürkan Aslan, Emrah Salman, Nilay Çöplü, Bedia Dinç

{"title":"Microbial isolates and resistance profiles in cerebrospinal fluid cultures: a five-year experience at a tertiary center.","authors":"Merve Gürler, Füsun Kırca, Figen Türen, İrem Tümkaya Kılınç, Ferhat Gürkan Aslan, Emrah Salman, Nilay Çöplü, Bedia Dinç","doi":"10.1080/17460913.2025.2520666","DOIUrl":"10.1080/17460913.2025.2520666","url":null,"abstract":"Purpose: Central nervous system infections are a significant global health concern that results in morbidity and mortality. In case of these infections, empirical antibiotic treatment should be initiated according to the microbiological findings of the cerebrospinal fluid (CSF), and the treatment should be rearranged according to the antibiogram results.The study aimed to determine the distribution of meningitis agents and antimicrobial resistance patterns in our hospital.Methods: A total of 17,594 CSF culture samples collected from January 2020 to December 2024 were analyzed retrospectively. The study assessed demographic data, causative microorganisms, and antimicrobial resistance rates.Results: Positive results were found in 7.3% of CSF culture samples, with 69.1% being gram-positive bacteria and 30.9% gram-negative bacteria. The highest positivity rate was detected in the palliative inpatient clinic (14.8%) despite most cultures being from the neonatal intensive care unit (23.5%). The most common microorganisms were coagulase-negative staphylococci(CoNS) (47.9%) and Klebsiella pneumoniae (K.pneumoniae) (9.1%). Methicillin resistance was present in 89.7% of CoNS, and the rate of extended-spectrum β-lactamase-producing strains in K.pneumoniae was 32.4%.Conclusion: Analysis of five-year data revealed the types of microorganisms and their antimicrobial resistance in positive CSF cultures. These findings will guide clinicians in promptly choosing the most effective empirical treatment.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"669-680"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12239795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro system for Taenia solium cysts culture and growth: progress toward a neurocysticercosis model. 猪带绦虫囊培养和生长的体外系统：神经囊虫模型的研究进展。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-07-01 Epub Date: 2025-06-06 DOI: 10.1080/17460913.2025.2516413

Miguel A Orrego, Oscar H Del Brutto, Carlos M Vasquez, Theodore E Nash, Hector H Garcia

{"title":"In vitro system for Taenia solium cysts culture and growth: progress toward a neurocysticercosis model.","authors":"Miguel A Orrego, Oscar H Del Brutto, Carlos M Vasquez, Theodore E Nash, Hector H Garcia","doi":"10.1080/17460913.2025.2516413","DOIUrl":"10.1080/17460913.2025.2516413","url":null,"abstract":"Aims: Subarachnoid neurocysticercosis is an aggressive form of Taeniasoliuminfection characterized by uncontrolled expansion of subarachnoid cysts. This study aims to investigate the role of exogenous lipidsand TGF-βin the proliferation of germinative cells and bladder wall expansion, providing insights into the biological mechanisms underlying cyst growth.Material & methods: Germinative cells and bladder wall sections discarded after surgical resection of subarachnoid cysts in humans were cultured in vitro under axenic conditions. The effects of TGF-βand exogenous lipids, both individually and combined, were assessed through proliferation assays and morphological analysis to determine their role in cyst expansion.Results: TGF-β-mediatedsignaling was found to be crucial for germinative cell replication, with lipid supply further enhancing bladder wall expansion. Long-term viability of cultured bladder wall sections was successfully maintained, enabling extended analysis of cyst biology.Conclusions: Thisinvitrosystem provides a powerful platform for characterizing the biology of subarachnoid cysts and for testing new therapeutic agents aimed at improving the treatment of human neurocysticercosis.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"631-637"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12239812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0