In vitro system for Taenia solium cysts culture and growth: progress toward a neurocysticercosis model.

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Future microbiology Pub Date : 2025-06-06 DOI:10.1080/17460913.2025.2516413

Miguel A Orrego, Oscar H Del Brutto, Carlos M Vasquez, Theodore E Nash, Hector H Garcia

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引用次数: 0

Abstract

Aims: Subarachnoid neurocysticercosis is an aggressive form of Taeniasoliuminfection characterized by uncontrolled expansion of subarachnoid cysts. This study aims to investigate the role of exogenous lipidsand TGF-βin the proliferation of germinative cells and bladder wall expansion, providing insights into the biological mechanisms underlying cyst growth.

Material & methods: Germinative cells and bladder wall sections discarded after surgical resection of subarachnoid cysts in humans were cultured in vitro under axenic conditions. The effects of TGF-βand exogenous lipids, both individually and combined, were assessed through proliferation assays and morphological analysis to determine their role in cyst expansion.

Results: TGF-β-mediatedsignaling was found to be crucial for germinative cell replication, with lipid supply further enhancing bladder wall expansion. Long-term viability of cultured bladder wall sections was successfully maintained, enabling extended analysis of cyst biology.

Conclusions: Thisinvitrosystem provides a powerful platform for characterizing the biology of subarachnoid cysts and for testing new therapeutic agents aimed at improving the treatment of human neurocysticercosis.

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猪带绦虫囊培养和生长的体外系统：神经囊虫模型的研究进展。

目的：蛛网膜下腔神经囊虫病是一种侵袭性带绦虫感染，其特征是蛛网膜下腔囊肿不受控制的扩张。本研究旨在探讨外源性脂质和TGF-β在萌发细胞增殖和膀胱壁扩张中的作用，为囊肿生长的生物学机制提供新的见解。材料与方法：在体外无菌条件下培养人蛛网膜下腔囊肿切除术后丢弃的生殖细胞和膀胱壁切片。通过增殖实验和形态学分析来评估TGF-β和外源性脂质单独或联合的作用，以确定它们在囊肿扩张中的作用。结果：发现TGF-β介导的信号传导对萌发性细胞复制至关重要，脂质供应进一步促进膀胱壁扩张。成功地维持了培养膀胱壁切片的长期生存能力，使囊肿生物学的扩展分析成为可能。结论：该体外系统为描述蛛网膜下腔囊肿的生物学特性和测试新的治疗药物提供了一个强大的平台，旨在改善人类神经囊虫病的治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Future microbiology 生物-微生物学

CiteScore

4.90

自引率

3.20%

发文量

134

审稿时长

6-12 weeks

期刊介绍： Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.