Future microbiology最新文献_第2页

Multidrug-resistant hypervirulent Klebsiella pneumoniae: an evolving superbug.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-26 DOI: 10.1080/17460913.2025.2482478

Yu Zheng, Xiaojue Zhu, Chao Ding, Weiqiang Chu, Xiaoxiao Pang, Ruxia Zhang, Jiucheng Ma, Guoxin Xu

引用次数: 0

Clinical Spectrum of Toxocariasis: A Retrospective Study From a Tertiary Care Center in Lebanon.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-13 DOI: 10.1080/17460913.2025.2477919

Nour El Meski, L'Emir Wassim El Ayoubi, Samar Hassani, Nayda Bidikian, Johnny Zakhour, Jad Bou Khalil, Zeina A Kanafani, Souha S Kanj

{"title":"Clinical Spectrum of Toxocariasis: A Retrospective Study From a Tertiary Care Center in Lebanon.","authors":"Nour El Meski, L'Emir Wassim El Ayoubi, Samar Hassani, Nayda Bidikian, Johnny Zakhour, Jad Bou Khalil, Zeina A Kanafani, Souha S Kanj","doi":"10.1080/17460913.2025.2477919","DOIUrl":"10.1080/17460913.2025.2477919","url":null,"abstract":"Aims: Toxocariasis is a prevalent zoonotic disease worldwide caused primarily by Toxocara canis and Toxocara cati. Despite its prevalence, studies focusing on clinical manifestations, and laboratory findings of toxocariasis in the Middle East North African (MENA) region are limited, highlighting a significant gap in research. The aim of this study is to describe the spectrum of toxocariasis observed at a tertiary center in Lebanon and review the geographic distribution of infected individuals and their demographics.Methods: The medical records of 225 patients who tested positive for Toxocara IgG via ELISA between 2002 and 2022 at the American University of Beirut Medical Center (AUBMC) were reviewed.Results: 141/225 patients had confirmed positive Toxocara-specific IgG by Western blot. These patients were mostly middle-aged, with a mean of 46 years and predominantly males. Most were Lebanese (95.3%), residing in Mount Lebanon or Beirut governates. Patients were mainly symptomatic (78.5%), and the most common presenting symptoms were neurological complaints. For those with Toxocara myelitis (23 patients), spine magnetic resonance imaging (MRI) findings showed focal lesions (54.5%) mostly at the thoracic vertebrae.Conclusions: Given the challenges associated with diagnosing this disease, further studies are essential to enhance our understanding of toxocariasis in the region.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"419-427"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of melanin in Fonsecaea monophora pathogenicity: adhesion, fibroblast interactions, and implications for chromoblastomycosis.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-20 DOI: 10.1080/17460913.2025.2480973

Tengteng Xin, Yushi Wu, Junmin Zhang

{"title":"The role of melanin in Fonsecaea monophora pathogenicity: adhesion, fibroblast interactions, and implications for chromoblastomycosis.","authors":"Tengteng Xin, Yushi Wu, Junmin Zhang","doi":"10.1080/17460913.2025.2480973","DOIUrl":"10.1080/17460913.2025.2480973","url":null,"abstract":"Aims: Fonsecaea monophora is a pathogen of chromoblastomycosis (CBM), and melanin is one of the important virulence factors of F.monophora. Despite the crucial role fibroblasts play in inflammatory diseases, the interaction between fibroblasts and F.monophora remains poorly understood. This study investigated fibroblast responses to wild-type (WT) and polyketide synthase A gene knockout (ΔpksA) strains, aiming to elucidate the role of fungal melanin in adhesion mechanisms and the interactions between fibroblasts and pathogens.Materials & methods: Coculture systems of fibroblasts with WT and ΔpksA strains of F.monophora were established. Adhesion and ectophosphatase activity assays evaluated fungal attachment. Cell scratch and Transwell migration assays assessed fibroblast motility. RT-qPCR and ELISA quantified collagen synthesis, myofibroblast differentiation, and apoptosis-related gene expression.Results and conclusions: The WT strain exhibited greater adhesion and inhibited fibroblast migration, whereas the ΔpksA strain showed reduced adhesion and enhanced migration, correlating with melanin content and ectophosphatase activity. Both strains inhibited fibroblast-to-myofibroblast differentiation, collagen synthesis, and extracellular matrix deposition, contributing to tissue remodeling. These findings highlight melanin's role in F. monophora's pathogenicity and its disruption of fibroblast function, potentially delaying wound healing in CBM.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"449-456"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repair capacity of Taenia solium extraparenchymal cysts: radiological and in vitro evidence.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-04 DOI: 10.1080/17460913.2025.2472594

Andrea Toledo, Pedro Tadao Hamamoto Filho, Marco A Gaona Corona, Diana Sierra León, Obet Jair Canela Calderon, Marco Antônio Zanini, Roger Carrillo Mezo, Agnès Fleury

{"title":"Repair capacity of Taenia solium extraparenchymal cysts: radiological and in vitro evidence.","authors":"Andrea Toledo, Pedro Tadao Hamamoto Filho, Marco A Gaona Corona, Diana Sierra León, Obet Jair Canela Calderon, Marco Antônio Zanini, Roger Carrillo Mezo, Agnès Fleury","doi":"10.1080/17460913.2025.2472594","DOIUrl":"10.1080/17460913.2025.2472594","url":null,"abstract":"Extraparenchymal neurocysticercosis (EP-NC) responds poorly to anthelmintic treatment. Several factors are involved in this low responsiveness, including the host's heterogeneous immune response and the ability of the parasite to evade it. In this study, we present radiological and in vitro findings that demonstrate that Taenia solium cysts have the capacity to repair from injuries. Six patients (three with cases of subarachnoid, two with cases of intraventricular, and one with a case of mixed subarachnoid and intraventricular cysts) presented with neurological complaints and underwent either medical or surgical treatment. Follow-up magnetic resonance imaging (MRI) showed apparent resolution of the cysts. However, months later (10-56) new MRI scans revealed cysts at the same sites observed before treatment. Cysts surgically removed were maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum. Monthly assessments demonstrated the growth of the parasites and the release of HP-10. Our findings demonstrate the ability of T. solium extraparenchymal cysts to grow and repair themselves. This capacity is likely another factor involved in the disease's poor treatment response.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"383-389"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143541152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ceftazidime-avibactam for the treatment of Pseudomonas aeruginosa: a plain language summary.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-30 DOI: 10.1080/17460913.2025.2479967

Pattarachai Kiratisin, Marie Kempf, Gregory Stone, Eric Utt

引用次数: 0

Unveiling antifungal resistance and biocide tolerance in clinical isolates of Candida spp.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-22 DOI: 10.1080/17460913.2025.2480924

Jamile De Paiva Macedo, Aripuana Aranha Sakurada Watanabe, Alessandra Barbosa Ferreira Machado, Cláudio Galuppo Diniz, Vania Lucia Da Silva, Vanessa Cordeiro Dias

{"title":"Unveiling antifungal resistance and biocide tolerance in clinical isolates of Candida spp.","authors":"Jamile De Paiva Macedo, Aripuana Aranha Sakurada Watanabe, Alessandra Barbosa Ferreira Machado, Cláudio Galuppo Diniz, Vania Lucia Da Silva, Vanessa Cordeiro Dias","doi":"10.1080/17460913.2025.2480924","DOIUrl":"10.1080/17460913.2025.2480924","url":null,"abstract":"Aims: Candidiasis, caused by Candida spp. is an opportunistic infection with significant healthcare risks, worsened by trends in antifungal resistance. This study aimed to evaluate the antifungal susceptibility profile, investigate resistance mechanisms, assess efflux pump activity, and examine biocide tolerance in clinical Candida isolates.Methods: A total of 100 Candida isolates from hospitalized and outpatient individuals were analyzed for their antifungal susceptibility profile, molecular resistance mechanisms through PCR, efflux pump activity with the Cartwheel method, and biocide tolerance (sodium hypochlorite, hydrogen peroxide, and benzalkonium chloride), which was assessed by disk diffusion.Results: A high prevalence of resistance (87%) to at least one antifungal was observed, with 47.12% of isolates showing simultaneous multiple resistance to three azole derivatives. The highest antifungal agent resistance was observed for fluconazole (n = 70) and the highest susceptibility for amphotericin B (n = 1). The most common mutation was in the ERG11 gene (n = 38/43.7%). Efflux pump activity was detected in both C. albicans and non-albicans Candida species. Biocide testing revealed a higher tolerance for sodium hypochlorite, with an inhibition zone ranging from 18.25 (4.40) to 34.0 (4.00).Conclusions: This study highlights significant antifungal resistance in Candida spp. particularly to azoles, stressing the need for improved infection control and novel therapeutic strategies.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"457-468"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143676911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prohibitins in infection: potential therapeutic targets.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-01 Epub Date: 2025-01-29 DOI: 10.1080/17460913.2025.2459530

Nuo Chen, Xiaolan Liu, Lulu Wang, Hui Yu, Fangqian Liu, Mengran Yuan, Qimeng Wang, Tianyi Zhang, Xiaoxiang Zhou, Hua Wang, Zengjun Ji, Hongxing Shen

{"title":"Prohibitins in infection: potential therapeutic targets.","authors":"Nuo Chen, Xiaolan Liu, Lulu Wang, Hui Yu, Fangqian Liu, Mengran Yuan, Qimeng Wang, Tianyi Zhang, Xiaoxiang Zhou, Hua Wang, Zengjun Ji, Hongxing Shen","doi":"10.1080/17460913.2025.2459530","DOIUrl":"10.1080/17460913.2025.2459530","url":null,"abstract":"Prohibitins (PHBs) are members of a highly conserved family of proteins, including prohibitin1 and prohibitin2. These proteins are predominantly localized in mitochondria, the nucleus, and cell membranes, where they play critical roles in mitochondrial biogenesis, apoptosis, immune regulation, and other biological processes. Recent studies have demonstrated that both PHB1 and PHB2 can act as a complex or independently to participate in the pathogen infection process. This review focuses on the regulatory roles of PHB1 and PHB2 in viral, bacterial, parasitic and fungal infections, providing a theoretical basis and innovative perspectives for a comprehensive understanding of the roles and mechanisms of PHB1 and PHB2 in the regulation of microbial infections. Due to exerting multiple functions, PHB proteins have been recognized as a potential target for therapeutic interventions, with the expectation that targeting PHB proteins will provide new strategies for the treatment of infection-related diseases.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"345-355"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trichosporon asahii: emerging challenges in pathogenesis and drug resistance.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-01 Epub Date: 2025-01-27 DOI: 10.1080/17460913.2025.2457858

Hanzhao Zhang, Jingping Zhang

{"title":"Trichosporon asahii: emerging challenges in pathogenesis and drug resistance.","authors":"Hanzhao Zhang, Jingping Zhang","doi":"10.1080/17460913.2025.2457858","DOIUrl":"10.1080/17460913.2025.2457858","url":null,"abstract":"Trichosporon asahii (T. asahii) is an opportunistic pathogenic fungus that often causes severe infections in immunosuppressed patients. Among Trichosporon species, T. asahii is the most pathogenic and lethal species. Current research faces challenges related to unknown pathogenic mechanisms, complex resistance mechanisms, insufficiently rapid and accurate diagnostic methods, and insufficient research on susceptibility to infection. These issues need to be explored in depth. This review summarizes research progress on the origin and classification of T. asahii, its virulence factors and pathogenic mechanisms, epidemiological characteristics, infection modes, diagnostic methods, drug treatment options, and drug resistance mechanisms. Traditional culture combined with molecular biology techniques, such as polymerase chain reaction and gene sequencing, has improved the accuracy and speed of detection. Treatment relies mainly on azole antifungal drugs and amphotericin B; however, patients are facing the problem of drug resistance. New techniques, such as gene knockout and gene sequencing, have identified resistance mechanisms, thus supporting the development of novel antifungal drugs. In summary, an in-depth study of T. asahii will aid in developing more effective diagnostic and therapeutic methods and improve patient prognosis.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"333-343"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Propionylation of Fis K32 in Salmonella enterica serovar Typhi: a key modification affecting pathogenicity.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-01 Epub Date: 2025-01-30 DOI: 10.1080/17460913.2025.2460338

Hao Tang, Ziyang Zhan, Xiucheng Liu, Xinxiang Huang

{"title":"Propionylation of Fis K32 in Salmonella enterica serovar Typhi: a key modification affecting pathogenicity.","authors":"Hao Tang, Ziyang Zhan, Xiucheng Liu, Xinxiang Huang","doi":"10.1080/17460913.2025.2460338","DOIUrl":"10.1080/17460913.2025.2460338","url":null,"abstract":"Aim: This study aims to explore the role of propionylation at the K32 residue of the global regulator Fis in Salmonella enterica serovar Typhi (S. Typhi) and its influence on the pathogenicity of the bacteria.Materials & methods: Bacterial strains were cultured in media with sodium propionate supplementation. The propionylation status of Fis was determined through Western blot and mass spectrometry analyses. The DNA-binding capability of Fis was assessed using EMSA. The invasion and survival capacities of S. Typhi were examined using T84 cells and THP-1 macrophages.Results: Propionylation at the K32 site of Fis was found to down-regulate its DNA-binding ability, leading to a reduction in the invasion and survival of S. Typhi within host cells. The K32Q mutant exhibited significantly decreased invasion and survival capabilities compared to the wild-type and K32R mutant strains.Conclusion: Propionylation of Fis at the K32 residue impacts the pathogenicity of S. Typhi, shedding light on the role of post-translational modifications in bacterial infections.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"295-303"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938949/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cefepime-enmetazobactam: first approved cefepime-β- lactamase inhibitor combination for multi-drug resistant Enterobacterales.

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-03-01 Epub Date: 2025-02-26 DOI: 10.1080/17460913.2025.2468112

Tanaya Bhowmick, Rafael Canton, Federico Pea, Juan Quevedo, Anne Santerre Henriksen, Jean-François Timsit, Keith S Kaye

{"title":"Cefepime-enmetazobactam: first approved cefepime-β- lactamase inhibitor combination for multi-drug resistant Enterobacterales.","authors":"Tanaya Bhowmick, Rafael Canton, Federico Pea, Juan Quevedo, Anne Santerre Henriksen, Jean-François Timsit, Keith S Kaye","doi":"10.1080/17460913.2025.2468112","DOIUrl":"10.1080/17460913.2025.2468112","url":null,"abstract":"Cefepime-enmetazobactam is a β-lactam/β-lactamase inhibitor (BL/BLI) combination that has demonstrated potent activity against extended spectrum beta-lactamase (ESBL)-producing Enterobacterales, recently approved by the US FDA for the treatment of complicated urinary tract infections (cUTI) and by the European Medicines Agency and the UK Healthcare products Regulatory Agency for the treatment of cUTI, hospital-acquired pneumonia including ventilator-associated pneumonia and bacteremia in adults. Cefepime is a 4th generation cephalosporin with a broad spectrum bactericidal activity and enhanced stability to degradation by chromosomal and plasmid-mediated AmpC cephalosporinases as well as carbapenemase OXA-48 like enzymes. Enmetazobactam is a novel penicillanic acid sulfone β-lactamase inhibitor structurally similar to tazobactam with activity against CTX-M, TEM, and SHV ESBL, and other class A β-lactamases, that restores cefepime activity in vitro and in vivo against ESBLs-producing Enterobacterales. The targeted activity of cefepime-enmetazobactam against ESBLs infections which are resistant to common antibiotics, together with its superior results against piperacillin/tazobactam in a phase 3 trial make this new BL/BLI combination an effective carbapenem sparing option for the treatment of serious infections caused by ESBLs-producing Enterobacterales. In addition, if clinical data are supportive, cefepime-enmetazobactam has a potential role in the treatment of pathogens co-producing OXA-48 like enzymes along with ESBLs.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"277-286"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0