Future microbiology最新文献

{"title":"A case report of fatal <i>Rickettsia japonica</i> bloodstream infection in Zhejiang, China.","authors":"Jianzhen Wang, Suqing Fan, Jing He, Xuxia Jiang, Rui Lin, Youqi Ji, Yumei Ge","doi":"10.1080/17460913.2024.2406122","DOIUrl":"https://doi.org/10.1080/17460913.2024.2406122","url":null,"abstract":"<p><p>Japanese spotted fever (JSF) is an emerging acute febrile natural infectious disease caused by the neglected zoonotic pathogen <i>Rickettsia japonica</i>. Here we reported a 64-year-old female patient who initially presented to the local hospital with an intermittent fever of unknown origin (FUO). A systemic, evident edema and eschar on the skin of the patient's upper limb was observed. The patient was diagnosed with critical <i>Rickettsia japonica</i> bloodstream infection by Q-mNGS and treated with doxycycline, as well as symptomatic treatments. Unfortunately, the patient passed away as a result of complications of septic shock and multiple organ and acute respiratory failure. Delayed treatment resulting from the nonspecific clinical symptoms in the early stages of infection can lead to fatal complications. Q-mNGS is an emerging pathogen detection method with the advantages of comprehensive detection, high accuracy and sensitivity and should be promoted and applied by clinicians.</p>","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142389913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic review of tuberculous meningitis in high-risk populations: mortality and diagnostic disparities.","authors":"Andressa Lorena Ieque, Carolina Trevisolli Palomo, Deborah de Castro Moreira, Jean Eduardo Meneguello, Letícia Sayuri Murase, Lincoln Luís Silva, Vanessa Pietrowski Baldin, Katiany Rizzieri Caleffi-Ferracioli, Vera Lucia Dias Siqueira, Rosilene Fressatti Cardoso, Jorge Juarez Vieira Teixeira, Regiane Bertin De Lima Scodro","doi":"10.1080/17460913.2024.2366604","DOIUrl":"10.1080/17460913.2024.2366604","url":null,"abstract":"<p><p><b>Aim:</b> Tuberculous meningitis (TBM) is one of the most severe clinical forms of tuberculosis (TB). Since epidemiological studies can contribute to TB control, we conducted a review and meta-analysis of epidemiological publications of adults TBM cases in countries with high incidence of TB.<b>Materials & methods:</b> The search resulted in 11,855 articles, in which 21 ultimately were included in our review and 15 in our meta-analysis.<b>Results:</b> TBM mortality was 25% with death rates of 70% in Africa. The review showed different and non-concordant diagnostic techniques and treatment schemes.<b>Conclusion:</b> Adults living in the African region are at high risk of death from TBM, highlighting an urgent need of guidelines to support diagnosis and treatment, and ultimately, to reduce mortality.</p>","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142365055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fecal microbiota spores, live-brpk (VOWST™/VOS) for prevention of recurrent <i>Clostridioides difficile</i> infection.","authors":"Parul Berry, Sahil Khanna","doi":"10.1080/17460913.2024.2403892","DOIUrl":"https://doi.org/10.1080/17460913.2024.2403892","url":null,"abstract":"<p><p><i>Clostridioides difficile</i> infection (CDI) is a health crisis comprising a majority of healthcare-associated infections and is now being seen in the community. Persistent dysbiosis despite treatment with standard-of-care antibiotics increases risk of recurrent infections. Fecal microbiota transplantation has been an effective way of addressing dysbiosis, but the studies have lacked standardization, which makes outcome and safety data difficult to interpret. Standardized microbiome therapies have demonstrated efficacy and safety for recurrent CDI and have been approved to prevent recurrent infection. In this review, we discuss the data behind and the practice use of fecal microbiota spores, live-brpk (VOWST™ / VOS), a US FDA approved live biotherapeutic for the prevention of recurrent CDI.</p>","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142345126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The anti-biofilm efficacy of copper and zinc doped borate bioactive glasses.","authors":"Sarah Fakher, David Westenberg","doi":"10.1080/17460913.2024.2398410","DOIUrl":"https://doi.org/10.1080/17460913.2024.2398410","url":null,"abstract":"<p><p><b>Aim:</b> Healthcare-acquired infections (HAIs) pose significant challenges in medical settings due to their resistance to conventional treatment methods. The role of bacterial biofilms in exacerbating these infections is well-documented, making HAIs particularly difficult to eradicate. Despite numerous research efforts, an effective solution to combat these infections remains elusive. This study aims to explore the potential of metal-ion (copper and zinc) doped borate bioactive glasses (BBGs) as a novel treatment modality to inhibit bacterial species commonly implicated in HAIs: <i>Staphylococcus epidermidis</i>, <i>Escherichia coli</i>, and <i>Pseudomonas aeruginosa</i>.<b>Methods:</b> The study analyzed the efficacy of both direct and indirect applications of BBGs on severe biofilms pre-formed under static and dynamic growth conditions; a comprehensive predictive modeling was developed, simulating diverse clinically relevant conditions.<b>Results:</b> Results demonstrate more than 4 log reduction in bacterial growth within 2 days for direct application and 3 days for indirect application of copper and zinc-doped BBGs. These findings were consistent across the three bacterial species, in both static and dynamic conditions.<b>Conclusion:</b> Copper and zinc-doped BBGs can be an effective approach in combating HAIs complicated by biofilms.</p>","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142284405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two 5-year studies of bictegravir/emtricitabine/tenofovir alafenamide in people with HIV: a plain-language summary.","authors":"Paul E Sax, Jason T Hindman, Hal Martin, David Wohl","doi":"10.1080/17460913.2024.2372231","DOIUrl":"https://doi.org/10.1080/17460913.2024.2372231","url":null,"abstract":"<p><strong>What is this summary about?: </strong>This is a plain-language summary of an article that reported on two studies of the medication bictegravir/emtricitabine/tenofovir alafenamide (shortened to B/F/TAF). B/F/TAF is a single pill containing three different drugs used to treat human immunodeficiency virus (known as HIV). The drugs work together to lower the levels of HIV (called viral load) in the body and make the virus undetectable in the blood. Researchers measured whether B/F/TAF was safe and effective when taken over 5 years in over 400 people in 10 countries who had never taken HIV medication before.</p><p><strong>What were the results?: </strong>After 5 years, almost all (99%) of the people who took B/F/TAF had an undetectable viral load. This does not mean that they were cured, but that the levels of HIV were so low that the tests used by researchers could not detect the virus in the blood. CD4 is a type of immune system cell. HIV causes CD4 cell numbers to decrease. On average, the number of CD4 cells increased by more than 300 cells per microliter (cells/μL) of blood over 5 years. This means that the immune system was improving. HIV is able to change its genes to escape the effects of the drugs. This is known as HIV resistance to treatment. Nine people had a viral load high enough to suggest that the drugs might not be working, but no resistance to B/F/TAF was seen. Some people (less than one in three) experienced medical problems thought to be linked to B/F/TAF treatment, known as side effects. The most common side effects were headache, diarrhea, nausea, tiredness (fatigue), dizziness, and difficulty falling or staying asleep (insomnia). On average, people's body weight increased by 3 kg in the first year of taking B/F/TAF. This might be because their general health improved after starting HIV treatment. Weight gained after that time was similar to the level of weight gain expected in the general population. Very few people (less than 1 in 100) stopped taking B/F/TAF because of side effects thought to have been caused by B/F/TAF.</p><p><strong>What do the results mean?: </strong>B/F/TAF was effective at treating HIV in people who had never taken HIV medication before. Most (70%) people were still taking B/F/TAF after 5 years.<b>Clinical Trial Registration:</b> NCT02607930 (Study 1489); NCT02607956 (Study 1490) (ClinicalTrials.gov).</p>","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-genomic next-generation sequencing in the diagnosis of brucellosis: Five cases from a non-endemic area.","authors":"Xuan Zhang, Xinfei Yao, Huixin Chen, Dongsheng Han, Meifang Yang","doi":"10.1080/17460913.2024.2363632","DOIUrl":"10.1080/17460913.2024.2363632","url":null,"abstract":"<p><p>Metagenomic next-generation sequencing (mNGS) in diagnosis of human brucellosis is comparatively unexplored. This report details five human brucellosis cases diagnosed using mNGS based on Illumina sequencing platform, comprising three females and two males, four with epidemiological exposure. In cases 1 and 2, plasma mNGS results showed one positive and one negative for <i>Brucella melitensis</i>, and subsequent blood cultures were both positive. Cases 3, 4 and 5 involved spinal brucellosis, some with paravertebral abscesses. mNGS from infectious tissue samples successfully detected <i>Brucella</i>, with read counts ranging between 30 and 1314, yet cultures were negative in cases 4 and 5. Following antibiotic and surgical treatments, all patients showed clinical improvement. This report shows mNGS testing enhances the detection sensitivity of brucellosis diagnosis.</p>","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529194/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141897285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mangiferin potentiates the activity of antifungal agents against fluconazole-resistant <i>Candida</i> spp.","authors":"Thais Lima Ferreira, Amanda Cavalcante Leitão, Lisandra Juvêncio da Silva, Livia Gurgel do Amaral Valente Sá, Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Sarah Alves Barbosa, João Batista de Andrade Neto, Amanda Dias Barbosa, Lara Elloyse Almeida Moreira, Maria Erivanda França Rios, Bruno Coêlho Cavalcanti, Manoel Odorico de Moraes, Hélio Vitoriano Nobre Júnior, Cecília Rocha da Silva","doi":"10.1080/17460913.2024.2366627","DOIUrl":"10.1080/17460913.2024.2366627","url":null,"abstract":"<p><p><b>Aim:</b> To evaluate the antifungal activity of mangiferin against <i>Candida</i> spp. resistant to fluconazole.<b>Materials & methods:</b> The antifungal activity of mangiferin was assessed using broth microdilution and its interaction with azoles and amphotericin B was evaluated by checkerboard. The activity of mangiferin against <i>Candida</i> spp. biofilms was assessed using the MTT colorimetric assay and its possible mechanism of action was evaluated using flow cytometry.<b>Results:</b> Mangiferin showed activity against <i>Candida albicans, Candida tropicalis</i> and <i>Candida parapsilosis</i> resistant to fluconazole and showed synergism with azoles and amphotericin B. Mangiferin increased the activity of antifungals against <i>Candida</i> biofilms and caused depolarization of the mitochondrial membrane and externalization of phosphatidylserine, suggesting apoptosis.<b>Conclusion:</b> mangiferin combined with antifungals has potential against <i>Candida</i> spp.</p>","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529198/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to the editor: accuracy required in data entry when using machine learning techniques to predict risk of disease.","authors":"Akash Korat, Dhaval Dobariya, Imtiyaz Bavaliya, Vikram Mali","doi":"10.1080/17460913.2024.2353525","DOIUrl":"10.1080/17460913.2024.2353525","url":null,"abstract":"","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141446069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiological analysis of tunneled hemodialysis catheters isolated from patients receiving hemodialysis in Saskatchewan.","authors":"Murugesan Sivaranjani, Haley Sanderson, Chinenye R Nnajide, Anna Martens-Koop, Joseph M Blondeau, Rodrick Stryker, Aaron P White","doi":"10.1080/17460913.2024.2359879","DOIUrl":"10.1080/17460913.2024.2359879","url":null,"abstract":"<p><p><b>Aim:</b> To compare the microbial communities inside hemodialysis catheters from symptomatic and asymptomatic patients to determine their differences.<b>Materials & methods:</b> Catheters (<i>n</i> = 41) were removed from patients in the Saskatchewan Health Authority over an 18-month period. The catheter section inside the body was flushed and the contents were evaluated using culture-dependent and culture-independent analysis.<b>Results:</b> All catheters were colonized by bacteria, with considerable overlap between groups based on microbial communities and the individual species detected. More Gram-negative species were detected by sequencing, whereas predominantly Gram-positive strains were cultured. Antibiotic resistance and biofilm formation was widespread and not correlated with either catheter group.<b>Conclusion:</b> Common pathogens were detected in each set of catheters, therefore predicting infections based on the microbiology is difficult.</p>","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141987824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fighting fire with fire: using infectious agents to treat persistent infection.","authors":"Maha Albukhari, Maria Bagies, Tanya Lizbeth, Shyamasundaran Kottilil","doi":"10.1080/17460913.2024.2363728","DOIUrl":"10.1080/17460913.2024.2363728","url":null,"abstract":"<p><p>Infectious diseases lead to significant morbidity and mortality. Often, resolution of the acute stage of the disease leads to microbial persistence, resulting in chronic debilitating disease. Management of persistent infections frequently requires lifelong therapy with antimicrobial agents. These infections could be chronic viral infections like HIV, hepatitis B or chronic bacterial persistent infections like prosthetic joint infections caused by multi-drug resistant organisms. Bacteriophages have been designed specifically to target recalcitrant bacterial infections, such as prosthetic joint infections with varying success. In this review, we describe the historic evolution of scenarios and risks associated with innovative therapy using infectious agents to treat other persistent infections.</p>","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529199/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141893255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}