Future microbiology最新文献_第2页

Surface display of LukF-PV on Bacillus subtilis spore for oral administration. 口服枯草芽孢杆菌LukF-PV表面显示。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-21 DOI: 10.1080/17460913.2025.2480933

Nhi Ny Nguyen, Lan Duong, Minh B Doan, Hoang Duc Nguyen

{"title":"Surface display of LukF-PV on Bacillus subtilis spore for oral administration.","authors":"Nhi Ny Nguyen, Lan Duong, Minh B Doan, Hoang Duc Nguyen","doi":"10.1080/17460913.2025.2480933","DOIUrl":"10.1080/17460913.2025.2480933","url":null,"abstract":"Aim: Bacillus subtilis spores are promising candidates for oral vaccine, serving as carriers for foreign proteins from pathogens. Here we utilized CotB, a spore coat protein, to anchor the LukF-PV from Staphylococcus aureus onto the surface of B. subtilis spores. Our goal was to generate a new B. subtilis strain and assess the capacity of the recombinant spore strain to induce antibody production in mice.Methods: The fusion sequence of cotB-lukF-PV was cloned into E. coli and transformed into B. subtilis HT800F. Colony PCR confirmed the generation of the recombinant strain. SporeELISA verified the display of LukF-PV. Mice were orally gavaged with the spore, and the production of IgA antibodies in feces and IgG in blood was evaluated.Results: We generated a new B. subtilis strain, BsHT2332, that integrated the PcotB-cotB-lukF-PV into its genome at amyE locus. BsHT2332 successfully displayed LukF-PV on the spore surface. The recombinant spores induced significant IgA and IgG immune responses in mice.Conclusion: This study demonstrated that B. subtilis spores expressing the S. aureus antigen LukF-PV can induce an immune response. These findings underscore the potential of the B. subtilis spore platform as a promising approach for vaccine development against S. aureus.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"469-477"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A family cluster of Brucella abortus infections possibly due to contact with a sika deer in Northeast China. 中国东北一例可能因接触梅花鹿而感染的流产布鲁氏菌家族聚集性感染。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-19 DOI: 10.1080/17460913.2025.2479297

Kun Li, Dawei Cheng, Zhifeng Xing, Yu Fan, Qingqing Xu, Xueying Chen, Meinan Li, Hongyan Zhao, Dongri Piao, Hai Jiang

引用次数: 0

Modelling the effects of temperature, pH and osmotic shifts on the autofluorescence of Staphylococcus aureus in vitro. 模拟温度、pH值和渗透变化对体外金黄色葡萄球菌自身荧光的影响。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-11 DOI: 10.1080/17460913.2025.2476875

Ao Du, Xiaofen Sun, Meili Dong, Yong Liu, Mingwei Chen, Yuhan Wang, Yuanzhi Zhang, Yao Huang, Zhongsheng Li, Xiang Huang, Yikun Wang, Jingshu Ni

{"title":"Modelling the effects of temperature, pH and osmotic shifts on the autofluorescence of Staphylococcus aureus in vitro.","authors":"Ao Du, Xiaofen Sun, Meili Dong, Yong Liu, Mingwei Chen, Yuhan Wang, Yuanzhi Zhang, Yao Huang, Zhongsheng Li, Xiang Huang, Yikun Wang, Jingshu Ni","doi":"10.1080/17460913.2025.2476875","DOIUrl":"10.1080/17460913.2025.2476875","url":null,"abstract":"Aims: This study aims to investigate how different wound microenvironmental factors (temperature, pH, and osmotic pressure) influence the autofluorescence of Staphylococcus aureus (S. aureus) and its underlying molecular mechanisms, specifically focusing on the porphobilinogen synthase gene (hemB) gene expression.Methods: We measured the average fluorescence intensity of S. aureus colonies under varying conditions of pH (3, 5, 7, 9, 11), temperature (25°C, 31°C, 37°C, 43°C), and osmotic pressure (0.9%, 1.8%, 2.7%, 3.6%) over time. Fluorescence intensity was quantified using ImageJ software. Additionally, RT-qPCR was used to analyze the expression levels of the hemB under these conditions.Results: Bacterial fluorescence intensity increased as the temperature ranged from 25°C to 43°C, with corresponding upregulation of hemB expression. At pH values between 3 and 11, fluorescence intensity decreased as pH increased, reflecting a similar trend in hemB expression. Fluorescence also diminished with higher osmotic pressures (0.9% to 3.6%), mirroring the downregulation of hemB.Conclusions: Our findings indicate that temperature, pH, and osmotic pressure significantly affect the autofluorescence of S. aureus by modulating porphyrin accumulation through hemB gene expression. These environmental factors should be considered when using bacterial fluorescence for wound infection assessment.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"409-418"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Not all disinfectants are created equal: the importance of mechanistic understanding to drive research forward. 并非所有消毒剂都是一样的：从机制上理解推动研究向前发展的重要性。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-17 DOI: 10.1080/17460913.2025.2480946

Elise L Bezold, Kevin P C Minbiole, William M Wuest

引用次数: 0

Correction. 修正。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-02-05 DOI: 10.1080/17460913.2025.2463265

引用次数: 0

Does Cryptococcus neoformans infection alter antifungal distribution: an animal model exploring pharmacokinetic changes. 新型隐球菌感染是否会改变抗真菌药的分布：一个探索药代动力学变化的动物模型。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-04-03 DOI: 10.1080/17460913.2025.2484956

Izabel Almeida Alves, Keli Jaqueline Staudt, Bruna Gaelzer Silva Torres, Calinca Elioterio Oliveira, Ryan Lago Araujo Rieper, Layza Kowalski, Bibiana Verlindo de Araújo

{"title":"Does Cryptococcus neoformans infection alter antifungal distribution: an animal model exploring pharmacokinetic changes.","authors":"Izabel Almeida Alves, Keli Jaqueline Staudt, Bruna Gaelzer Silva Torres, Calinca Elioterio Oliveira, Ryan Lago Araujo Rieper, Layza Kowalski, Bibiana Verlindo de Araújo","doi":"10.1080/17460913.2025.2484956","DOIUrl":"10.1080/17460913.2025.2484956","url":null,"abstract":"Aim: Assessing the disseminated meningitis caused by Cryptoccocus neoformans in Wistar rats and its impact on antifungal distribution by microdialysis (µD).Materials & methods: The yeast presence was investigated in different tissues by histological and microbiological assays, and biochemical parameters such as urea, glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), creatinine, creatine kinase (CK), creatinine, albumin level, leukocyte counts, and brain vascular permeability (Evans blue test) were evaluated in healthy and infected groups. Levels of fluconazole reached in the animal's brain were determined by µD.Results: Differences in albumin, urea, GPT, and CK between healthy and infected animals were observed in the levels of Evans blue as well as in the brain (0.51 vs 1.50 µg/gbrain). The drugs' distribution in the brain of infected animals was higher than that in the brain of healthy ones (ft = 1.37 vs ft = 0.54).Conclusion: The model validated presents characteristics similar to those observed in patients and can be applied to pharmacokinetic investigations.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"489-498"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multidrug-resistant hypervirulent Klebsiella pneumoniae: an evolving superbug. 多药耐药高致病性肺炎克雷伯菌：一种进化中的超级细菌。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-26 DOI: 10.1080/17460913.2025.2482478

Yu Zheng, Xiaojue Zhu, Chao Ding, Weiqiang Chu, Xiaoxiao Pang, Ruxia Zhang, Jiucheng Ma, Guoxin Xu

引用次数: 0

The role of melanin in Fonsecaea monophora pathogenicity: adhesion, fibroblast interactions, and implications for chromoblastomycosis. 黑色素在金黄色葡萄球菌致病性中的作用：粘附、成纤维细胞相互作用和对嗜色菌病的影响。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-20 DOI: 10.1080/17460913.2025.2480973

Tengteng Xin, Yushi Wu, Junmin Zhang

{"title":"The role of melanin in Fonsecaea monophora pathogenicity: adhesion, fibroblast interactions, and implications for chromoblastomycosis.","authors":"Tengteng Xin, Yushi Wu, Junmin Zhang","doi":"10.1080/17460913.2025.2480973","DOIUrl":"10.1080/17460913.2025.2480973","url":null,"abstract":"Aims: Fonsecaea monophora is a pathogen of chromoblastomycosis (CBM), and melanin is one of the important virulence factors of F.monophora. Despite the crucial role fibroblasts play in inflammatory diseases, the interaction between fibroblasts and F.monophora remains poorly understood. This study investigated fibroblast responses to wild-type (WT) and polyketide synthase A gene knockout (ΔpksA) strains, aiming to elucidate the role of fungal melanin in adhesion mechanisms and the interactions between fibroblasts and pathogens.Materials & methods: Coculture systems of fibroblasts with WT and ΔpksA strains of F.monophora were established. Adhesion and ectophosphatase activity assays evaluated fungal attachment. Cell scratch and Transwell migration assays assessed fibroblast motility. RT-qPCR and ELISA quantified collagen synthesis, myofibroblast differentiation, and apoptosis-related gene expression.Results and conclusions: The WT strain exhibited greater adhesion and inhibited fibroblast migration, whereas the ΔpksA strain showed reduced adhesion and enhanced migration, correlating with melanin content and ectophosphatase activity. Both strains inhibited fibroblast-to-myofibroblast differentiation, collagen synthesis, and extracellular matrix deposition, contributing to tissue remodeling. These findings highlight melanin's role in F. monophora's pathogenicity and its disruption of fibroblast function, potentially delaying wound healing in CBM.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"449-456"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980502/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Spectrum of Toxocariasis: A Retrospective Study From a Tertiary Care Center in Lebanon. 弓形虫病的临床谱：来自黎巴嫩三级保健中心的回顾性研究。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-13 DOI: 10.1080/17460913.2025.2477919

Nour El Meski, L'Emir Wassim El Ayoubi, Samar Hassani, Nayda Bidikian, Johnny Zakhour, Jad Bou Khalil, Zeina A Kanafani, Souha S Kanj

{"title":"Clinical Spectrum of Toxocariasis: A Retrospective Study From a Tertiary Care Center in Lebanon.","authors":"Nour El Meski, L'Emir Wassim El Ayoubi, Samar Hassani, Nayda Bidikian, Johnny Zakhour, Jad Bou Khalil, Zeina A Kanafani, Souha S Kanj","doi":"10.1080/17460913.2025.2477919","DOIUrl":"10.1080/17460913.2025.2477919","url":null,"abstract":"Aims: Toxocariasis is a prevalent zoonotic disease worldwide caused primarily by Toxocara canis and Toxocara cati. Despite its prevalence, studies focusing on clinical manifestations, and laboratory findings of toxocariasis in the Middle East North African (MENA) region are limited, highlighting a significant gap in research. The aim of this study is to describe the spectrum of toxocariasis observed at a tertiary center in Lebanon and review the geographic distribution of infected individuals and their demographics.Methods: The medical records of 225 patients who tested positive for Toxocara IgG via ELISA between 2002 and 2022 at the American University of Beirut Medical Center (AUBMC) were reviewed.Results: 141/225 patients had confirmed positive Toxocara-specific IgG by Western blot. These patients were mostly middle-aged, with a mean of 46 years and predominantly males. Most were Lebanese (95.3%), residing in Mount Lebanon or Beirut governates. Patients were mainly symptomatic (78.5%), and the most common presenting symptoms were neurological complaints. For those with Toxocara myelitis (23 patients), spine magnetic resonance imaging (MRI) findings showed focal lesions (54.5%) mostly at the thoracic vertebrae.Conclusions: Given the challenges associated with diagnosing this disease, further studies are essential to enhance our understanding of toxocariasis in the region.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"419-427"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980464/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repair capacity of Taenia solium extraparenchymal cysts: radiological and in vitro evidence. 猪带绦虫肝实质外囊肿的修复能力：放射学和体外证据。

IF 2.5 4区生物学

Future microbiology Pub Date : 2025-04-01 Epub Date: 2025-03-04 DOI: 10.1080/17460913.2025.2472594

Andrea Toledo, Pedro Tadao Hamamoto Filho, Marco A Gaona Corona, Diana Sierra León, Obet Jair Canela Calderon, Marco Antônio Zanini, Roger Carrillo Mezo, Agnès Fleury

{"title":"Repair capacity of Taenia solium extraparenchymal cysts: radiological and in vitro evidence.","authors":"Andrea Toledo, Pedro Tadao Hamamoto Filho, Marco A Gaona Corona, Diana Sierra León, Obet Jair Canela Calderon, Marco Antônio Zanini, Roger Carrillo Mezo, Agnès Fleury","doi":"10.1080/17460913.2025.2472594","DOIUrl":"10.1080/17460913.2025.2472594","url":null,"abstract":"Extraparenchymal neurocysticercosis (EP-NC) responds poorly to anthelmintic treatment. Several factors are involved in this low responsiveness, including the host's heterogeneous immune response and the ability of the parasite to evade it. In this study, we present radiological and in vitro findings that demonstrate that Taenia solium cysts have the capacity to repair from injuries. Six patients (three with cases of subarachnoid, two with cases of intraventricular, and one with a case of mixed subarachnoid and intraventricular cysts) presented with neurological complaints and underwent either medical or surgical treatment. Follow-up magnetic resonance imaging (MRI) showed apparent resolution of the cysts. However, months later (10-56) new MRI scans revealed cysts at the same sites observed before treatment. Cysts surgically removed were maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum. Monthly assessments demonstrated the growth of the parasites and the release of HP-10. Our findings demonstrate the ability of T. solium extraparenchymal cysts to grow and repair themselves. This capacity is likely another factor involved in the disease's poor treatment response.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"383-389"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143541152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0