Surface display of LukF-PV on Bacillus subtilis spore for oral administration.

IF 2.5 4区 生物学 Q3 MICROBIOLOGY
Nhi Ny Nguyen, Lan Duong, Minh B Doan, Hoang Duc Nguyen
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引用次数: 0

Abstract

Aim: Bacillus subtilis spores are promising candidates for oral vaccine, serving as carriers for foreign proteins from pathogens. Here we utilized CotB, a spore coat protein, to anchor the LukF-PV from Staphylococcus aureus onto the surface of B. subtilis spores. Our goal was to generate a new B. subtilis strain and assess the capacity of the recombinant spore strain to induce antibody production in mice.

Methods: The fusion sequence of cotB-lukF-PV was cloned into E. coli and transformed into B. subtilis HT800F. Colony PCR confirmed the generation of the recombinant strain. SporeELISA verified the display of LukF-PV. Mice were orally gavaged with the spore, and the production of IgA antibodies in feces and IgG in blood was evaluated.

Results: We generated a new B. subtilis strain, BsHT2332, that integrated the PcotB-cotB-lukF-PV into its genome at amyE locus. BsHT2332 successfully displayed LukF-PV on the spore surface. The recombinant spores induced significant IgA and IgG immune responses in mice.

Conclusion: This study demonstrated that B. subtilis spores expressing the S. aureus antigen LukF-PV can induce an immune response. These findings underscore the potential of the B. subtilis spore platform as a promising approach for vaccine development against S. aureus.

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来源期刊
Future microbiology
Future microbiology 生物-微生物学
CiteScore
4.90
自引率
3.20%
发文量
134
审稿时长
6-12 weeks
期刊介绍: Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.
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