Antimicrobial action and mechanism of piscidin against Helicobacter pylori.

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Future microbiology Pub Date : 2025-04-22 DOI:10.1080/17460913.2025.2491253

Yunhai Wei, Wenjing Hu, Huancen Guo, Jinyu Zhang, Haiyan Dong, Chu Shen, Jingrui Cao, Zhongxin Wu, Lei Yin, Yuhai Gao

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引用次数: 0

Abstract

Background: The rise of antibiotic-resistant Helicobacter pylori (Hp) strains is complicating the management of common gastrointestinal conditions such as gastritis and ulcers. In search of effective treatment options, antimicrobial peptides are being explored as a promising strategy.

Methods: Leveraging bioinformatics, we dissected structural and sequence profiles of piscidin, an antimicrobial peptide. We investigated whether long-term piscidin exposure induces resistance in Hp strains by measuring their minimum inhibitory concentration. The Oxford cup test was applied to measure inhibition zones of piscidin against Hp strains. Following piscidin treatment, we monitored morphological alterations in Hp strains using scanning electron microscopy, and changes in urease activity, expressions of virulence factors CagA and UreB, and adenosine triphosphate levels. Cytotoxicity of piscidin was evaluated in AGS and 293T cells.

Results: Piscidin inhibited proliferation of Hp strains, with a low propensity of inducing resistance. Delving into underlying mechanisms, we observed piscidin could alter morphology of Hp strains, suppress urease activity, and downregulate expressions of virulence factors CagA and UreB, while also reducing intracellular ATP levels. Piscidin had good security.

Conclusion: Piscidin is a potent antibacterial agent against Hp with low resistance risk, making it a safe and effective treatment candidate.

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鱼青素对幽门螺杆菌的抑菌作用及机制研究。

背景：耐抗生素幽门螺杆菌（Hp）菌株的增加使胃炎和溃疡等常见胃肠道疾病的治疗复杂化。为了寻找有效的治疗方案，抗菌肽作为一种有前途的策略正在被探索。方法：利用生物信息学，我们剖析了piscidin（一种抗菌肽）的结构和序列。我们通过测量其最低抑制浓度来研究长期暴露于鱼毒苷是否会诱导Hp菌株产生耐药性。采用牛津杯法测定了鱼青素对Hp的抑制范围。在鱼毒苷处理后，我们用扫描电镜观察了Hp菌株的形态学变化，以及脲酶活性、毒力因子CagA和UreB的表达和三磷酸腺苷水平的变化。在AGS和293T细胞中评价鱼毒苷的细胞毒性。结果：鱼青素抑制Hp菌株的增殖，诱导耐药倾向较低。深入研究其潜在机制，我们发现piscidin可以改变Hp菌株的形态，抑制脲酶活性，下调毒力因子CagA和UreB的表达，同时降低细胞内ATP水平。双鱼座有很好的安全保障。结论：Piscidin是一种有效的Hp抗菌药物，耐药风险低，是一种安全有效的治疗Hp的候选药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Future microbiology 生物-微生物学

CiteScore

4.90

自引率

3.20%

发文量

134

审稿时长

6-12 weeks

期刊介绍： Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.