Frontiers in Veterinary Science最新文献

{"title":"Dietary niacin supplementation improves meat quality, muscle fiber type, and mitochondrial function in heat-stressed Taihe black-bone silky fowls.","authors":"Wenliang Mei, Wenyan Zhang, Ziyu Hu, Mingren Qu, Gen Wan, Xiaoquan Guo, Chuanbin Chen, Lanjiao Xu","doi":"10.3389/fvets.2024.1491553","DOIUrl":"10.3389/fvets.2024.1491553","url":null,"abstract":"<p><strong>Background: </strong>A recent study has shown that niacin supplementation induces the conversion of type II to type I muscle fibres, thereby promoting a phenotypic shift in oxidative metabolism in porcine skeletal muscle. These effects may be mediated by modulation of the AMPK1/SIRT1 pathway, which activates peroxisome proliferator-activated receptor <i>γ</i> coactivator-1α (PGC-1α), a key regulator of fibre conversion, thereby promoting skeletal muscle mitochondrial biogenesis and myofibre conversion. In this study, we explored how niacin (NA) supplementation impacts the quality of meat and the characteristics of muscle fibers in Taihe Black-bone Silky Fowls (TBsf) exposed to heat conditions.</p><p><strong>Methods: </strong>Chickens were rationally assigned to five different treatment groups with five replicates of six chickens each: thermophilic (TN), heat stress (HS) and HS + NA (HN) groups, with the HN group being supplemented with 200, 400 and 800 mg/kg (HS + NA_0.02, HS + NA_0.04 and HS + NA_0.08) NA in the premix, respectively.</p><p><strong>Results: </strong>The results of the experiment showed that addition of 800 mg/kg NA to the diet significantly improved TBsf muscle tenderness compared to HS. Dietary enrichment with 200-800 mg/kg NA significantly increased total antioxidant capacity, superoxide dismutase, and glutathione peroxidase activities, while significantly decreasing malondialdehyde compared to HS. Incorporation of 200-800 mg/kg NA into the diet significantly reduced lactate dehydrogenase activity and myosin heavy chain (MyHC-IIB) gene expression. Furthermore, adding 800 mg/kg NA can significantly enhance the mRNA expression of mitochondrial transcription factors (TFAM and TFB1M) in TBsf skeletal muscle. Adding 400 and 800 mg/kg of NA significantly increased the mRNA expression of AMP-activated protein kinase 1 (AMPK1), PGC-1α, cytochrome c oxidase (Cytc), and nuclear respiratory factor (NRF-1) in the skeletal muscle of TBsf. Supplementing NA at 200-400 mg/kg significantly increased the expression of Sirtuin 1 (SIRT1) mRNA in TBsf skeletal muscle.</p><p><strong>Conclusion: </strong>The experimental results showed that the addition of NA to the diet reduced the shear force of TBsf muscle under heat exposure conditions. It increased the proportion of type I muscle fibres by increasing the antioxidant capacity of the muscle and by promoting mitochondr fibreial biogenesis. Considering the results of this study, it is recommended that TBsf be supplemented with 400-800 mg/kg of NA in the diet to reduce the adverse effects of heat stress on meat quality.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11513878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new herbal anesthetic agent for common carp (<i>Cyprinus carpio</i>) sedation and anesthesia: nutmeg (<i>Myristica fragrans</i>) essential oil.","authors":"Mert Minaz","doi":"10.3389/fvets.2024.1477357","DOIUrl":"10.3389/fvets.2024.1477357","url":null,"abstract":"<p><p>In aquaculture, interest in natural essential oils is increasing alongside synthetic anesthetic agents. In this context, the anesthetic efficacy of nutmeg essential oil, which had not been previously tested, was investigated in common carp (<i>Cyprinus carpio</i>). The study, conducted using three different concentrations (800 μL/L \"LC\", 1,200 μL/L \"MC\", and 1,400 μL/L \"HC\"), found that induction times were <3 min for MC and HC, while LC had a longer induction time, exceeding acceptable levels. Within the first 4 h, white blood cells, red blood cells, hemoglobin, and hematocrit levels increased to >5 10³/μL, >1 10⁶/μL, >6 g/dL, and >12%, respectively. However, they returned to control levels after 8 h. Histological signs were more severe with higher concentrations, and necrosis was only observed in the HC group. Alkaline comet assay results showed DNA migration only in the HC group. According to the PROMETHEE multi-criteria decision-making model, the LC concentration is suitable for sedation, while the MC concentration should be used for deep anesthesia. The current study demonstrates that nutmeg essential oil can be used as an alternative to commercial synthetic anesthetic agents.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11514786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of genetic resources and trends of omics applications in donkey research: focus on China.","authors":"Muhammad Zahoor Khan, Wenting Chen, Xinrui Wang, Huili Liang, Lin Wei, Bingjian Huang, Xiyan Kou, Xiaotong Liu, Zhenwei Zhang, Wenqiong Chai, Adnan Khan, Yongdong Peng, Changfa Wang","doi":"10.3389/fvets.2024.1366128","DOIUrl":"10.3389/fvets.2024.1366128","url":null,"abstract":"<p><p>Omics methodologies, such as genomics, transcriptomics, proteomics, metabolomics, lipidomics and microbiomics, have revolutionized biological research by allowing comprehensive molecular analysis in livestock animals. However, despite being widely used in various animal species, research on donkeys has been notably scarce. China, renowned for its rich history in donkey husbandry, plays a pivotal role in their conservation and utilization. China boasts 24 distinct donkey breeds, necessitating conservation efforts, especially for smaller breeds facing extinction threats. So far, omics approaches have been employed in studies of donkey milk and meat, shedding light on their composition and quality. Similarly, omics methods have been utilized to explore the molecular basis associated with donkey growth, meat production, and quality traits. Omics analysis has also unraveled the critical role of donkey microbiota in health and nutrition, with gut microbiome studies revealing associations with factors such as pregnancy, age, transportation stress, and altitude. Furthermore, omics applications have addressed donkey health issues, including infectious diseases and reproductive problems. In addition, these applications have also provided insights into the improvement of donkey reproductive efficiency research. In conclusion, omics methodologies are essential for advancing knowledge about donkeys, their genetic diversity, and their applications across various domains. However, omics research in donkeys is still in its infancy, and there is a need for continued research to enhance donkey breeding, production, and welfare in China and beyond.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11502298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the comparative efficacy of green lipped mussel plus krill oil extracts (EAB-277), <i>Biota orientalis</i> extracts or NSAIDs for the treatment of dogs with osteoarthritis associated pain: a blinded, placebo-controlled study.","authors":"Naruepon Kampa, Duangdaun Kaenkangploo, Supranee Jitpean, Thanikul Srithunyarat, Suvaluk Seesupa, Somphong Hoisang, Karn Yongvanit, Phanthit Kamlangchai, Pongsatorn Tuchpramuk, B Duncan X Lascelles","doi":"10.3389/fvets.2024.1464549","DOIUrl":"https://doi.org/10.3389/fvets.2024.1464549","url":null,"abstract":"<p><strong>Introduction: </strong>With little to no regulation of the supplement markets and a paucity of quality information regarding clinical utility of individual marketed supplements, it is difficult for veterinarians to provide any evidence-based recommendations to owners. The current study aimed to provide clinically useful comparative efficacy data on certain marketed supplements.</p><p><strong>Methods: </strong>Using a prospective, block-randomized, double-blinded, placebo-controlled design, one hundred and one pet dogs with clinical hip OA-associated pain with one side worse than the other (index limb) were randomly assigned to one of four treatment groups: Green lipped Mussel plus Krill oil extracts (Antinol® Rapid, EAB-277); <i>Biota orientalis</i> extracts (4CYTE™ Epiitalis® Forte); an NSAID (meloxicam); or placebo (sunflower oil). Peak vertical force (PVF, expressed as a percentage of bodyweight) of the index limb, orthopedic assessment score (OAS) and hematology and blood chemistry values were evaluated before treatment (week 0), at 2, 4 and 6 weeks during treatment.</p><p><strong>Results: </strong>At 6 weeks, the changes from baseline in PVF of the index limb in the EAB-277 and meloxicam groups were significantly greater than the change in the placebo and 4CYTE™ groups, and the placebo and 4CYTE groups were not different from each other. At 6 weeks, there were significant differences between the groups for overall OAS scores with the lowest scores (least impairment) in the EAB-277 and meloxicam groups, followed by the 4CYTE group and then the placebo group.</p><p><strong>Discussion: </strong>Results of this study indicate that meloxicam and EAB-277 have significant objectively measured benefits in managing OA-related pain in dogs compared to placebo, but 4CYTE does not differ from placebo.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500327/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accuracy and validation of a point-of-care blood glucose monitoring system for use in horses.","authors":"Sridhar Velineni, Paul Schiltz, Ko-Hsin Chang, Yi-Ming Peng, Bobby Cowles","doi":"10.3389/fvets.2024.1436714","DOIUrl":"https://doi.org/10.3389/fvets.2024.1436714","url":null,"abstract":"<p><p>Abnormal blood glucose (BG) levels often seen in critically ill horses are significantly associated with adverse patient outcomes and increased mortality. Rapid and accurate BG monitoring is now considered an essential component of evidence-based equine practice and can provide critical information quickly for treatment. Although several point-of-care (POC) BG monitoring hand-held devices are commercially available for veterinary use, none contains a unique algorithm validated for use in horses. The AlphaTrak 3 (AT3) BG monitoring system is a first-of-its-kind device with an equine-specific algorithm that allows stall-side clinical decision making, and frequent monitoring at minimal cost. As such, AT3 is potentially a preferred alternative to more costly and time-consuming standard diagnostic reference laboratory methods. The objective of this study was to determine the accuracy of the AT3 device in measuring BG levels in equine whole blood samples in comparison to results obtained by the Beckman Coulter AU480 reference analyzer per ISO15197:2013 specifications. Accuracy of the AT3 equine algorithm were initially verified by testing equine blood samples with artificially adjusted blood glucose levels followed by its validation in a field study. Testing with artificially adjusted equine samples (<i>n</i> = 129) showed that 98.9% of glucose measurements ranging from 29 to 479 mg/dL fell within ISO accuracy threshold of ±15 mg/dL or ±15% of the average reference value. In addition, 100% of the AT3 measurements fell in consensus error grid (CEG) zone A, which indicates that test outcomes have a minimal likelihood of adverse clinical impact. In a follow-up field study involving 96 horses, 98.4% of AT3 measurements met the ISO accuracy threshold and 99.2% of AT3 measurements fell in CEG zone A. These results demonstrate that the AT3 glucometer has a high degree of accuracy in horses and is a dependable, convenient, and cost-effective device for accurately monitoring equine BG levels in farm or clinical settings.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500461/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of yeast peptides on the growth, antioxidant and anti-inflammatory capacities, immune function, and diarrhea status of suckling calves.","authors":"Xuexian Liu, Jiashu Yang, Yibo Yan, Kai Wang, Chunyan Guo","doi":"10.3389/fvets.2024.1454839","DOIUrl":"https://doi.org/10.3389/fvets.2024.1454839","url":null,"abstract":"<p><p>Yeast peptides, which are small-molecule active peptides extracted from yeast proteins, are known for their antibacterial, antioxidant, and anti-inflammatory properties. However, the effects of yeast peptide on suckling calves remain unclear. In this study, the effects of yeast peptide supplementation on the growth, diarrhea incidence, and immune function of calves during the suckling period were determined. Thirty newborn calves were randomly divided into two groups: the control group (CON) and the treatment group (AP), which received fresh pasteurized milk supplemented with yeast peptides (5 g/day). The experiment lasted for 49 days (7-56 days of age). The dry matter intake, body weight, diarrhea status, immune function, antioxidant capacity, and anti-inflammatory activity of the calves were analyzed. The AP group had higher dry matter intake, daily weight gain, and feed efficiency than the CON group (<i>P</i> < 0.05). Moreover, the duration and frequency of diarrhea were significantly lower in the AP group than in the CON group (<i>P</i> < 0.05). Furthermore, the immune, antioxidant, and anti-inflammatory capabilities of the AP group were significantly higher than those of the CON group (<i>P</i> < 0.05). These findings provide valuable insights for the improvement of early health management during calf rearing.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11499939/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of propionate-producing bacterial consortium in ruminal methanogenesis inhibited environment with bromoethanesulfonate as a methanogen direct inhibitor.","authors":"Jongsik Jeong, Chaemin Yu, Ryukseok Kang, Myunghoo Kim, Tansol Park","doi":"10.3389/fvets.2024.1422474","DOIUrl":"https://doi.org/10.3389/fvets.2024.1422474","url":null,"abstract":"<p><p>Methane production in ruminants is primarily due to the conversion of metabolic hydrogen (H₂), produced during anaerobic microbial fermentation, into methane by ruminal methanogens. While this process plays a crucial role in efficiently disposes of H₂, it also contributes to environmental pollution and eliminating methane production in the rumen has proven to be challenging. This study investigates the use of probiotics, specifically propionate-producing bacteria, to redirect accumulated H₂ in a methane-mitigated environment. For this objective, we supplemented experimental groups with <i>Lactiplantibacillus plantarum</i> and <i>Megasphaera elsdenii</i> for the reinforced acrylate pathway (RA) and <i>Selenomonas ruminantium</i> and <i>Acidipropionibacterium thoenii</i> for the reinforced succinate pathway (RS), as well as a consortium of all four strains (CB), with the total microbial concentration at 1.0 × 10¹⁰ cells/mL. To create a methane-mitigated environment, 2-bromoethanesulfonate (BES) was added to all experimental groups at a dose of 15 mg/0.5 g of feed. BES reduced methane production by 85% <i>in vitro</i>, and the addition of propionate-producing bacteria with BES further decreased methane emission by up to 94% compared with the control (CON) group. Although BES did not affect the alpha diversity of the ruminal bacteriome, it reduced total volatile fatty acid production and altered beta diversity of ruminal bacteriota, indicating microbial metabolic adaptations to H₂ accumulation. Despite using different bacterial strains targeting divergent metabolic pathways (RA and RS), a decrease in the dominance of the [<i>Eubacterium</i>] <i>ruminantium</i> group suggesting that both approaches may have a similar modulatory effect. An increase in the relative abundance of <i>Succiniclasticum</i> in the CB group suggests that propionate metabolism is enhanced by the addition of a propionate-producing bacterial consortium. These findings recommend using a consortium of propionate-producing bacteria to manage H₂ accumulation by altering the rumen bacteriome, thus mitigating the negative effects of methane reduction strategies.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11497462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fish welfare in farms: potential, knowledge gaps and other insights from the fair-fish database.","authors":"Caroline Marques Maia, João Luis Saraiva, Eliane Gonçalves-de-Freitas","doi":"10.3389/fvets.2024.1450087","DOIUrl":"https://doi.org/10.3389/fvets.2024.1450087","url":null,"abstract":"<p><p>An adequate understanding of fish behaviors and their interaction with farm-specific environments is pivotal for enhancing fish welfare in aquaculture. The fair-fish database aims to provide a consistent overview of the welfare of farmed fish. This platform consolidates ethological knowledge into profiles of farmed aquatic species. Its WelfareCheck profiles are organized around welfare indicators, with each criterion receiving classifications (no findings, unclear, low, medium, and high) regarding the likelihood and potential for individuals of a given species to experience good welfare in aquaculture systems, along with the associated certainty level. These criteria include home range, depth range, migration patterns, reproduction, aggregation patterns, aggressive behavior, substrate needs, stress responses, malformations, and slaughtering protocols. We investigated which of these 10 criteria are most relevant to the overall welfare of a species, considering the likelihood, potential, and certainty of good welfare in aquaculture. To achieve this, we reviewed and recorded the high classifications across each criterion and dimension from all published WelfareCheck profiles. To further investigate knowledge gaps across the criteria, we also recorded classifications marked as <i>unclear</i> and <i>no findings</i>. These were then compared across the criteria to assess the frequency of such classifications. While no significant differences were found between the criteria regarding the likelihood that the surveyed species meet their basic welfare needs, criteria related to reproduction, slaughter practices, and substrate needs demonstrated a high potential for better welfare outcomes. Moreover, reproduction and migration patterns exhibited high certainty in the available literature. Based on these findings, we conclude that improving the reproduction of farmed aquatic species, considering their natural needs and behavior, could be an effective and reliable approach to improving welfare. However, we also found a low certainty of information on aggression and an absence or conflicting data on home range, aggregation patterns, stress, and malformations. This highlights an urgent need for research in these areas, which are fundamental for developing more accurate assessments and recommendations for farmed aquatic species.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11496955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global A-to-I RNA editing during myogenic differentiation of goat MuSCs.","authors":"Xiaoli Xu, Mancheng Zhang, Siyuan Zhan, Yuan Chen, Chengqi Wei, Jiaxue Cao, Jiazhong Guo, Dinghui Dai, Linjie Wang, Tao Zhong, Hongping Zhang, Li Li","doi":"10.3389/fvets.2024.1439029","DOIUrl":"https://doi.org/10.3389/fvets.2024.1439029","url":null,"abstract":"<p><strong>Background: </strong>RNA editing, especially A-to-I editing sites, is a common RNA modification critical for stem cell differentiation, muscle development, and disease occurrence. Unveiling comprehensive RNA A-to-I editing events associated with myogenesis of the skeletal muscle satellite cells (MuSCs) is essential for extending our knowledge of the mechanism underpinning muscle development.</p><p><strong>Results: </strong>A total of 9,632 RNA editing sites (RESs) were screened in the myoblasts (GM), myocytes (DM1), and myotubes (DM5) samples. Among these sites, 4,559 A-to-I edits were classified and further analyzed. There were 3,266 A-to-I sites in the protein-coding region, out of which 113 missense sites recoded protein. Notably, five A-to-I sites in the 3' UTR of four genes (<i>TRAF6</i>, <i>NALF1</i>, <i>SLC38A1</i>, <i>ENSCHIG00000019092</i>) altered their targeted miRNAs. Furthermore, a total of 370 A-to-I sites with different editing levels were detected, including <i>FBN1</i>, <i>MYH10</i>, <i>GSK3B</i>, <i>CSNK1D</i>, and <i>PRKACB</i> genes. These genes were predominantly enriched in the cytoskeleton in muscle cells, the hippo signaling pathway, and the tight junction. Furthermore, we identified 14 hub genes (<i>TUFM</i>, <i>GSK3B</i>, <i>JAK2</i>, <i>RPSA</i>, <i>YARS1</i>, <i>CDH2</i>, <i>PRKACB</i>, <i>RUNX1</i>, <i>NOTCH2</i>, <i>CDC23</i>, <i>VCP</i>, <i>FBN1</i>, <i>RARS1</i>, <i>MEF2C</i>) that potentially related to muscle development. Additionally, 123 stage-specific A-to-I editing sites were identified, with 43 sites in GM, 25 in DM1, and 55 in DM5 samples. These stage-specific edited genes significantly enriched essential biological pathways, including the cell cycle, oocyte meiosis, motor proteins, and hedgehog signaling pathway.</p><p><strong>Conclusion: </strong>We systematically identified the RNA editing events in proliferating and differentiating goat MuSCs, which was crucial for expanding our understanding of the regulatory mechanisms of muscle development.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11496035/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periodontal ligament stem cell tissue engineering scaffolds can guide and promote canine periodontal tissue regeneration.","authors":"Pengxiu Dai, Guixiang Qi, Mingde Zhu, Qingjie Du, Keyi Wang, Yaxin Gao, Mengnan Li, Xiancheng Feng, Xinke Zhang","doi":"10.3389/fvets.2024.1465879","DOIUrl":"https://doi.org/10.3389/fvets.2024.1465879","url":null,"abstract":"<p><strong>Background: </strong>The immunogenicity of allogeneic mesenchymal stem cells (MSCs) is significantly enhanced after transplantation or differentiation, and these cells can be recognized and cleared by recipient immune cells. Graft rejection has become a major obstacle to improving the therapeutic effect of allogeneic MSCs or, after their differentiation, transplantation in the treatment of diabetes and other diseases. Solving this problem is helpful for prolonging the time that cells play a role in the recipient body and for significantly improving the clinical therapeutic effect.</p><p><strong>Methods: </strong>In this study, canine adipose-derived mesenchymal stem cells (ADSCs) were used as seed cells, and gene editing technology was used to knock out the B2M gene in these cells and cooperate with the overexpression of the PD-L1 gene. Gene-edited ADSCs (GeADSCs), whose biological characteristics and safety are not different from those of normal canine ADSCs, have been obtained.</p><p><strong>Results: </strong>The immunogenicity of GeADSCs is reduced, the immune escape ability of GeADSCs is enhanced, and GeADSCs can remain in the body for a longer time. Using the optimized induction program, the efficiency of the differentiation of GeADSCs into new islet β-cells was increased, and the maturity of the new islet β-cells was increased. The immunogenicity of new islet β-cells decreased, and their immune escape ability was enhanced after the cells were transplanted into diabetic dogs (the graft site was prevascularized by the implantation of a scaffold to form a vascularized pouch). The number of infiltrating immune cells and the content of immune factors were decreased at the graft site.</p><p><strong>Conclusions: </strong>New islet β-cell transplantation, which has low immunogenicity, can reverse diabetes in dogs, and the therapeutic effect of cell transplantation is significantly enhanced. This study provides a new method for prolonging the survival and functional time of cells in transplant recipients and significantly improving the clinical therapeutic effect.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11496256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}