Frontiers in Veterinary Science最新文献

{"title":"Optimizing thyroxine levels for enhanced buffalo sperm cryopreservation and fertility: a focus on quality, viability, and antioxidant protection.","authors":"Maha S Salama, Mohey A Ashour, Ahmed M Shehabeldin, Mohamed E A Omar, Mohamed M Soliman, Heba I Ghamry, Mohamed Abdelmegeid, Mustafa Shukry, Ahmed A Elolimy","doi":"10.3389/fvets.2025.1584903","DOIUrl":"https://doi.org/10.3389/fvets.2025.1584903","url":null,"abstract":"<p><strong>Introduction: </strong>This study investigated the effects of adding thyroxine (T4) to buffalo semen on sperm quality, oxidative markers, apoptosis-like changes, and fertility.</p><p><strong>Methods: </strong>Initially, we tested a wide range of T4 concentrations (0.1, 0.3, 0.9, 2.7, and 8.1 µg/dL) to evaluate their impact on motility and viability.</p><p><strong>Result and discussion: </strong>Lower concentrations (0.1-0.9 µg/dL) improved total and progressive motility and viability compared to higher concentrations (2.7 and 8.1 µg/dL). We assessed optimized doses (0.25, 0.5, 0.75, and 1 µg/dL) based on these findings. The 0.75 µg/dL group showed superior sperm velocity, viability, motion parameters, membrane, acrosome, and DNA integrity in equilibrated and frozen-thawed samples. Antioxidant markers (GPx, SOD, TAC) were enhanced, while MDA and apoptotic/necrotic cell levels were reduced, particularly in the 0.75 µg/dL group. Fertility trials revealed higher cryosurvival and conception rates in thyroxine-treated groups. In conclusion, T4 supplementation, especially at 0.75 µg/dL, enhances cryopreservation outcomes and fertility potential of buffalo bull semen.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1584903"},"PeriodicalIF":2.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol inhibits the formation of <i>Staphylococcus aureus</i> biofilms by reducing PIA, eDNA release, and ROS production.","authors":"Jinfei He, Yilong Cui, Yan Liu, Jingdong Mao, Yanxin Dong, Ruizhi Yao, Dahan Yang, Peichao Fan, Jiangdong Xue","doi":"10.3389/fvets.2025.1594239","DOIUrl":"https://doi.org/10.3389/fvets.2025.1594239","url":null,"abstract":"<p><strong>Introduction: </strong><i>Staphylococcus aureus</i> is a zoonotic pathogen that is difficult to control. Resveratrol (RES) has been shown to have significant antibacterial effects. The present study aimed to investigate the inhibitory effect of RES on the formation of <i>Staphylococcus aureus</i> biofilms and their molecular mechanism.</p><p><strong>Methods: </strong>First, the minimum inhibitory concentration and inhibitory action curve of RES against <i>Staphylococcus aureus</i> were obtained through testing. Second, we found that RES can inhibit biofilm formation by reducing the release of polysaccharide intercellular adhesion (PIA) and extracellular DNA (eDNA) from <i>Staphylococcus aureus</i>.</p><p><strong>Results: </strong>RES treatment significantly reduced the production of reactive oxygen species (ROS) and nicotinamide adenine dinucleotide phosphate (NADPH) in <i>Staphylococcus aureus</i>, indicating that ROS and NADPH are closely related to biofilm formation.</p><p><strong>Conclusion: </strong>This study demonstrates that RES inhibits the formation of <i>Staphylococcus aureus</i> biofilms by reducing PIA, eDNA release, and ROS production, and these results provide new ideas for the clinical application of RES in the treatment of <i>Staphylococcus aureus</i> infection.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1594239"},"PeriodicalIF":2.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human papillomavirus in canine serum: evidence from a Chinese study.","authors":"Yumeng Liu, Lulu Xie, Yimin Zhou, Lin Zhou, Jingshan Bi, Min Zheng, Tian Lan, Wenchao Sun","doi":"10.3389/fvets.2025.1511289","DOIUrl":"https://doi.org/10.3389/fvets.2025.1511289","url":null,"abstract":"<p><strong>Introduction: </strong>Human papillomaviruses (HPVs) are well-known for causing both benign and malignant epithelial growths in humans, but their occurrence in non-human species is rarely reported. Expanding the understanding of HPV's host range is essential for assessing its ecological and public health implications.</p><p><strong>Methods: </strong>We investigated serum samples from dogs collected in Guangxi, China, between 2014 and 2020. PCR screening was performed to detect HPV DNA, followed by sequencing and phylogenetic analysis of the positive amplicons.</p><p><strong>Results: </strong>HPV DNA was unexpectedly detected in 2 out of 1,226 canine serum samples, yielding a detection rate of 0.16%. Phylogenetic analysis revealed that the sequences clustered with alpha2-HPV78 (GX-70-related) and alpha2-HPV94 (GX-47-related), respectively. Interestingly, both sequences displayed multiple amino acid variations in viral proteins. However, virus isolation was not achieved.</p><p><strong>Discussion: </strong>The detection of HPV nucleic acids in canine serum suggests a potential presence of HPV in canine hosts. These findings provide new insights into the possible host range of HPV, underscoring the need for further research to assess the virus's infectivity, transmission dynamics, and implications for both animal and human health.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1511289"},"PeriodicalIF":2.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measuring the impact of sow farm outbreaks with PRRS virus on the downstream mortality using causal inference methods.","authors":"E S Magalhães, D Zhang, C A A Moura, Annette O'Connor, C Wang, D J Holtkamp, G S Silva, D C L Linhares","doi":"10.3389/fvets.2025.1545034","DOIUrl":"https://doi.org/10.3389/fvets.2025.1545034","url":null,"abstract":"<p><p>Porcine reproductive and respiratory syndrome virus (PRRSV) remains a significant challenge to the swine industry, resulting in substantial productivity and, consequently, economic losses. This study aimed to quantify the impact of PRRSV outbreaks in sow farms on nursery mortality using causal inference methods. The study design followed a retrospective observational approach, where PRRSV epidemic status in source sow farms was the exposure, and nursery mortality (percentage of dead pigs in the first 60 days post-weaning) was the outcome. Causal inference techniques were employed to estimate the effect of the exposure (PRRSV epidemic status) on the outcome (nursery mortality). Data from a Midwestern US swine production system comprising 2,592 lots of pigs, representing approximately 5 million pigs marketed between January 2021 and December 2022, were analyzed. A causal diagram was constructed to visualize the relationship between PRRSV epidemic exposure and nursery mortality, while controlling for potential confounding factors including season, average parity at farrow, and sow farm <i>Mycoplasma hyopneumoniae</i> status. Four analytical approaches were employed: univariate and multivariable regression models, propensity score matching, and a doubly robust method. The results indicated that PRRSV epidemic lots had higher nursery mortality compared to non-epidemic lots, regardless of the modeling approach used. The doubly robust method provided the most accurate estimates, offering lower mortality differences and narrower confidence intervals. This study demonstrated the application of causal inference methods on swine data to measure the impact of PRRSV on swine nursery mortality, which is an approach commonly used in other epidemiology areas but not well explored in veterinary epidemiology. The findings highlight the importance of employing causal inference models in veterinary epidemiology to improve the accuracy of disease impact assessments in field conditions, with potential applications in studying other pathogens or disease-related factors in livestock production.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1545034"},"PeriodicalIF":2.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multidrug-resistant <i>Proteus mirabilis</i> in a critically endangered Malayan pangolin: clinical and genomic insights.","authors":"Ziqiao Chen, Jiayi Wang, Kai Wang, Fuyu An, Shasha Liu, Haikuo Yan, Yan Hua","doi":"10.3389/fvets.2025.1552499","DOIUrl":"https://doi.org/10.3389/fvets.2025.1552499","url":null,"abstract":"<p><p><i>Proteus mirabilis</i>, an important zoonotic opportunistic pathogen, is widely found in nature and the intestinal tracts of animals, which can cause diarrhea, pneumonia, urinary tract infections, and other symptoms in domestic animals including sheep, pigs, cattle and chickens. In this study, necropsy of a deceased critically endangered Malayan pangolin revealed lobar pneumonia in the lungs and hepatocyte necrosis with hepatic cord disintegration in the liver. A strain of <i>Proteus mirabilis</i> (PM2022) was isolated from the affected lungs and liver. This bacterium exhibited multidrug resistance, being susceptible only to cefoxitin and amikacin. Whole-genome sequencing identified 26 antibiotic resistance genes, including <i>CTX-M-65</i>, <i>FosA3</i>, which mediate resistance to five classes of antibiotics, such as penicillins and quinolones. Additionally, 20 virulence factors (including the T6SS secretion system, hemolysins HpmA/B, among others) were detected. Mouse experiments confirmed its high pathogenicity (LD₅₀ = 1.45 × 10⁹ CFU/mL). Based on experimental and genomic testing results, the initial symptoms of <i>Proteus mirabilis</i> infection in pangolins manifest in the lungs, liver, and intestines, and the use of penicillins and quinolones should be avoided during treatment. This study offers clinical guidance for diagnosing and treating <i>P. mirabilis</i> infections in pangolins, informing evidence-based antimicrobial strategies.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1552499"},"PeriodicalIF":2.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A snapshot on molecular technologies for diagnosing FAdV infections.","authors":"Amina Kardoudi, Fellahi Siham, Allaoui Abdelmounaaim, Kichou Faouzi, Ouchhour Ikram, Jackson Thomas, Benani Abdelouaheb","doi":"10.3389/fvets.2025.1558257","DOIUrl":"https://doi.org/10.3389/fvets.2025.1558257","url":null,"abstract":"<p><p>Fowl adenoviruses (FAdV) are prevalent in chickens worldwide, responsible for several poultry diseases, including inclusion body hepatitis (IBH), hepatitis-hydropericardium syndrome (HHS), and gizzard erosion (GE), which result in significant economic losses in the poultry industry. Consequently, detection and efficient identification of FAdV serotypes are becoming extremely urgent to monitor outbreaks and develop vaccination strategies. Conventional PCR (cPCR) tests, combined with Restriction Fragment Length Polymorphism (RFLP) or sequencing, were developed for FAdV diagnosis. Although these molecular tests have considerably improved the accuracy of FAdV diagnosis compared with conventional methods, certain drawbacks remain unresolved, including lack of sensitivity and post-PCR analysis. Subsequently, advanced molecular technologies such as real-time PCR (qPCR), Loop Isothermal Amplification (LAMP), Cross-Priming Amplification (CPA), Recombinase Polymerase Amplification (RPA), Digital Droplet Polymerase Chain Reaction (ddPCR), Dot Blot Assay Combined with cPCR, Nanoparticle-Assisted PCR (nano-PCR), PCR-Refractory Quantitative Amplification (ARMS-qPCR), CRISPR/Cas13a Technology, and High-Resolution Melting Curve (HRM), have been developed to improve FAdV diagnosis.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1558257"},"PeriodicalIF":2.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial indices quantifying exposure to swine farming in North Carolina.","authors":"Kaushi S T Kanankege, Rashmi Kandwal, Andres M Perez","doi":"10.3389/fvets.2025.1552028","DOIUrl":"https://doi.org/10.3389/fvets.2025.1552028","url":null,"abstract":"<p><strong>Introduction: </strong>Proximity to swine farms is often used as a surrogate in exposure assessments, allowing for the relative quantification of potential pollutant dispersion, odor intensity, and health impacts on neighboring communities. However, defining exposure is complex, and the resulting risk profiles can vary depending on the definition used.</p><p><strong>Methods: </strong>To quantify the spatially based exposure of surrounding communities to swine farms in North Carolina, three spatially explicit metrics were developed at the census tract-level: IDx1: number of households within 1-mile from a hog farm, IDx2: Co-kriging using the number of hogs and manure lagoons, and IDx3: hog density per square mile. Then, the correlation between these indices and Centers for Disease Control and Prevention (CDC)'s Social Vulnerability Index (SVI) and Environmental Justice Index (EJI), which are generalized vulnerability measures, was evaluated to assess direct impact from swine farms versus multiple stressors.</p><p><strong>Results: </strong>The three indices differed visually, with IDx3 strongly correlated with IDx1 (0.8) and moderately correlated with IDx2 (0.4). CDC EJI and SVI were not prominently correlated with any of the swine-farm specific indices (≤0.3) indicating limited overlap. The correlation between swine-farm-specific indices and CDC SVI was slightly pronounced in rural areas indicating socially vulnerable populations are more likely to live near swine farming areas in rural census tracts. Having swine farm-specific indices offers a more tailored and nuanced understanding of the potential health and environmental risks. However, the differences between the maps and the varying correlations underscored how different definitions of exposure can yield distinct narratives about which neighborhoods are at risk. Defining and measuring potential exposure, considering factors like proximity, duration, frequency, vulnerability, and cumulative impact, is highly challenging.</p><p><strong>Discussion: </strong>The study emphasizes the need for a hierarchical framework to quantify and compare environmental exposures, addressing risk-modifying factors and individual-level exposure across space and time before implying direct exposure risks. This approach enables more informed planning for targeted solutions and fosters collaboration among stakeholders, facilitating critical discussions on integrated One Health solutions.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1552028"},"PeriodicalIF":2.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-hospital evaluation of an app-based seizure detection system in dogs: timely detection of generalized tonic-clonic seizures.","authors":"Junya Hirashima, Miyoko Saito, Daisuke Hasegawa, Rikako Asada, Masato Kitagawa, Daisuke Ito, Shinichi Kanazono, Koichi Fujiwara","doi":"10.3389/fvets.2025.1558274","DOIUrl":"10.3389/fvets.2025.1558274","url":null,"abstract":"<p><p>The seizure detection system (SDS) is a wearable device developed by us to detect generalized tonic-clonic seizures (GTCSs) in dogs with epilepsy. In our previous study, a feasibility test was conducted for the SDS, which demonstrated its ability to correctly identify three GTCSs in one dog. To enhance user accessibility and facilitate real-time monitoring of epileptic seizures in dogs, we integrated the system into a smartphone application. This study aimed to evaluate the performance of the app-based SDS in a clinical setting involving a larger number of dogs. Initially, the app-based SDS was tested on a laboratory dog with no history of seizures, and a drug-induced GTCS was accurately detected. Subsequently, an in-hospital evaluation was conducted. A total of 12 dogs were included, comprising 10 dogs with epilepsy, either hospitalized or temporarily housed at participating veterinary hospitals, and two laboratory dogs with epilepsy. In total, 34 GTCSs occurred in four of the 12 dogs, and the app-based SDS correctly detected 25 of the 34 GTCSs. Including the preliminary test results, the overall sensitivity of the app-based SDS was 74.3% (26 out of 35 GTCSs). Two false positives were observed in both in one dog. The false-positive rate and positive predictive value of the app-based SDS for detection of GTCS were 0.018 events/day and 92.6%, respectively. The median detection latency from the onset of a GTCS was 11 s. This study demonstrates that the app-based SDS is effective for detecting GTCSs in hospitalized dogs in clinical settings.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1558274"},"PeriodicalIF":2.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12069445/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum: Incorporating video telehealth for improving at-home management of chronic health conditions in cats: a focus on chronic mobility problems.","authors":"","doi":"10.3389/fvets.2025.1612906","DOIUrl":"https://doi.org/10.3389/fvets.2025.1612906","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fvets.2025.1510006.].</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1612906"},"PeriodicalIF":2.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070227/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143975865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-occurrence of feline chronic gingivostomatitis and oral squamous cell carcinoma in 4 cats (2014-2024).","authors":"Anson J Tsugawa, Maria M Soltero-Rivera, Stephanie Goldschmidt, Boaz Arzi, Tessa Kell, Naomi Hoyer, Cynthia M Bell, Hanzhi Gao, Guogen Shan, Natalia Vapniarsky","doi":"10.3389/fvets.2025.1564674","DOIUrl":"10.3389/fvets.2025.1564674","url":null,"abstract":"<p><strong>Introduction: </strong>Cats with refractory feline chronic gingivostomatitis (FCGS) exhibit chronic oral inflammation despite surgical and medical therapy. Such areas may resemble or be at higher risk for oral squamous cell carcinoma (SCC). Without routine biopsies, occult SCC may remain undiagnosed.</p><p><strong>Objectives: </strong>This study investigated the prevalence and potential association of oral SCC occurrence in cats with refractory FCGS.</p><p><strong>Methods: </strong>A retrospective review of cats with refractory FCGS and oral SCC from two veterinary teaching hospitals (2014-2024) was conducted. Cases with histopathologically confirmed FCGS, SCC, or both were included. Data analyzed included signalment, medical history, treatment, clinical findings, and diagnostics.</p><p><strong>Results: </strong>Two hundred twenty-one cats with refractory FCGS and 24 cats with oral SCC at the first institution, and 32 cats with refractory FCGS and 16 cats with oral SCC at the second institution, were presented over a 10-year period. Only four cats from both institutions had co-occurrence of FCGS and oral SCC. All affected cats exhibited bilateral proliferative FCGS lesions in the caudal oral cavity and developed SCC within 16-29 months (mean: 22 months). Two of four cats had epithelial dysplasia at FCGS diagnosis. SCC occurrence was significantly, inversely associated with FCGS at one institution (0.9%, <i>ρ</i> = -0.1424, <i>p</i>-value = 0.00035) but not the other (5.88%, <i>ρ</i> = 0.0495, <i>p</i>-value = 0.1947).</p><p><strong>Conclusion: </strong>Co-occurrence of FCGS and SCC is rare. While SCC may develop in proliferative FCGS areas, the low occurrence does not establish FCGS as a predisposing factor for SCC.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1564674"},"PeriodicalIF":2.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}