Isolation and identification of Mycoplasma hyorhinis and virulence evaluation of its field isolates.

IF 2.6 2区农林科学 Q1 VETERINARY SCIENCES

Frontiers in Veterinary Science Pub Date : 2025-06-18 eCollection Date: 2025-01-01 DOI:10.3389/fvets.2025.1542992

Fan Yang, Lijun Yang, Xuecheng Duan, Yulin Qian, Huifang Ma, Xue Jia, Xinyu Huo, Wenqi Dong, Huanchun Chen, Chen Tan

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引用次数: 0

Abstract

Introduction: As a prevalent swine pathogen worldwide, Mycoplasma hyorhinis (M. hyorhinis, Mhr) is associated with various diseases, including multiple serositis, pneumonia, arthritis, and otitis media. It is also linked to the porcine respiratory disease complex (PRDC).

Methods: M. hyorhinis prevalence in 2022 Chinese lung samples was assessed by species-specific PCR, followed by isolation and purification of field strains, followed by genetic characterization via multilocus sequence typing (MLST). Pathogenicity evaluation of three isolates (ZZ-1, GD-1 and AH-1) was evaluated using controlled piglet infection trials.

Results: Mhr detection in clinical lung samples showed 31.77% prevalence. Three isolates (ZZ-1/ST166, GD-1/ST167, AH-1/ST144) were characterized by MLST. Piglet infection trials confirmed Mhr-induced polyserositis, pneumonia, and arthritis, with strain-dependent virulence variation observed.

Discussion: This study confirms M. hyorhinis as a high-prevalence pathogen (31.77%) in Chinese swine herds. Animal infection models demonstrated virulence variation among different Mhr strains. These findings contribute to identifying and assessing the threats posed by different strains to pig health, guiding the development of clinical prevention and control strategies.

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嗜血杆菌支原体的分离鉴定及野外分离株的毒力评价。

作为一种世界范围内流行的猪病原体，缩耳支原体（m.h orhinis, Mhr）与多种疾病有关，包括多发性浆膜炎、肺炎、关节炎和中耳炎。它还与猪呼吸道疾病复合体（PRDC）有关。方法：采用种特异性PCR方法对2022例中国人肺标本中嗜鼻支原体的流行情况进行评估，并对现场菌株进行分离纯化，采用多位点序列分型（MLST）进行遗传鉴定。采用对照仔猪感染试验对3株分离株（ZZ-1、GD-1和AH-1）进行致病性评价。结果：临床肺标本Mhr检出率为31.77%。3株分离株（ZZ-1/ST166、GD-1/ST167、AH-1/ST144）经MLST鉴定。仔猪感染试验证实由mrh引起的多浆液炎、肺炎和关节炎，并观察到菌株依赖的毒力变异。讨论：本研究证实了猪嗜血杆菌在中国猪群中的高流行率（31.77%）。动物感染模型显示不同Mhr菌株的毒力存在差异。这些发现有助于识别和评估不同菌株对猪健康构成的威胁，指导临床预防和控制策略的制定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Veterinary Science Veterinary-General Veterinary

CiteScore

4.80

自引率

9.40%

发文量

1870

审稿时长

14 weeks

期刊介绍： Frontiers in Veterinary Science is a global, peer-reviewed, Open Access journal that bridges animal and human health, brings a comparative approach to medical and surgical challenges, and advances innovative biotechnology and therapy. Veterinary research today is interdisciplinary, collaborative, and socially relevant, transforming how we understand and investigate animal health and disease. Fundamental research in emerging infectious diseases, predictive genomics, stem cell therapy, and translational modelling is grounded within the integrative social context of public and environmental health, wildlife conservation, novel biomarkers, societal well-being, and cutting-edge clinical practice and specialization. Frontiers in Veterinary Science brings a 21st-century approach—networked, collaborative, and Open Access—to communicate this progress and innovation to both the specialist and to the wider audience of readers in the field. Frontiers in Veterinary Science publishes articles on outstanding discoveries across a wide spectrum of translational, foundational, and clinical research. The journal''s mission is to bring all relevant veterinary sciences together on a single platform with the goal of improving animal and human health.