Frontiers in Veterinary Science最新文献

{"title":"The complete mitochondrial genomes of <i>Macrostylophora euteles</i> and <i>Citellophilus tesquorum sungaris</i> and the phylogenetics of known Siphonaptera mitogenomes.","authors":"Mingna Duan, Yafang Liu, Jun Wu, Shuang Liu, Shaobo Tang, Dandan Jiang, Quanfu Zhang, Wei Gu, Xing Yang","doi":"10.3389/fvets.2025.1558328","DOIUrl":"https://doi.org/10.3389/fvets.2025.1558328","url":null,"abstract":"<p><p>Fleas serve as hosts to a diverse array of pathogens, which present significant medical and veterinary concerns for human and livestock health. The mitochondrial genome (mtDNA) has long been regarded as a classical model in biogenetics and species evolution research. However, the availability of mitochondrial genome data for fleas remains scarce. In this study, we sequenced <i>Macrostylophora euteles</i> specimens collected from the Yunnan plague focus and <i>Citellophilus tesquorum sungaris</i> specimens from Jilin plague focus. The obtained sequences were compared to the sequences of 24 flea species retrieved from the NCBI database, focusing on base composition, evolution rates, nucleotide polymorphism and phylogenetic analysis. All fleas analyzed contained a total of 37 genes. Gene sequences exhibited remarkable stability, with no evidence of gene rearrangement. Additionally, the base composition demonstrated a pronounced AT bias. Results from both methodologies and across the two datasets consistently indicated strong monophyly for the superfamilies Ceratophylloidea and Pulicoidea, as well as for the family Pulicidae. In contrast, the superfamily Hystrichopsylloidea, along with the families Ceratophyllidae, Leptopsyllidae and Ctenophthalmidae, were identified as paraphyletic. This research provides valuable molecular data to support taxonomic and phylogenetic studies of fleas.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1558328"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetic evaluation and bioavailability of KPT-335 (Verdinexor) in cats.","authors":"Yuxin Yang, Jinyan Meng, Zeyu Wen, Jianzhong Wang, Xingyuan Cao","doi":"10.3389/fvets.2025.1576669","DOIUrl":"https://doi.org/10.3389/fvets.2025.1576669","url":null,"abstract":"<p><p>KPT-335 (Verdinexor) is a novel, orally bioavailable selective inhibitor of nuclear export that has gained significant attention in pharmaceutical research due to its potential anti-tumor and antiviral effects. This study aimed to evaluate the pharmacokinetic parameters and determine the absolute bioavailability of KPT-335 through various administration routes, including oral capsules and tablets, along with intravenous injections. The intravenous group received a dosage of 1 mg/kg body weight (BW), while capsules were administered orally at doses of 0.2, 1, and 2 mg/kg BW. Tablets were also administered orally at 1 and 2 mg/kg BW, with both post-feeding and fasting conditions at the 1 mg/kg BW dosage. Plasma concentrations of KPT-335 were analyzed using ultra-performance liquid chromatography/tandem mass spectrometry. Key pharmacokinetic parameters, including peak concentration (C_max), area under the curve (AUC_0-last), and terminal phase elimination half-life (T_1/2), were determined through non-compartmental analysis using WinNonlin 8.1. The absolute bioavailability rates of 43.72, 44.66, and 28.92% for the low, medium, and high-dose capsule groups, respectively. In the tablet formulation, bioavailability at 1 mg/kg BW (fasting), 1 mg/kg BW (feeding), and 2 mg/kg BW (feeding) were 75.92, 70.98, and 47.27%, respectively. KPT-335 demonstrated pharmacokinetic characteristics of rapid absorption and elimination. The results demonstrated that KPT-335 exhibited non-linear pharmacokinetic behavior, indicating that higher doses are not fully absorbed in cats. This finding provides data support for guiding clinical dosing regimens. At the same dose, the absolute bioavailability of the tablet group was higher than that of the capsule group.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1576669"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Emerging challenges in companion animal toxicology.","authors":"Nicola Bates, Lynn Rolland Hovda, Kristin Opdal Seljetun","doi":"10.3389/fvets.2025.1610134","DOIUrl":"https://doi.org/10.3389/fvets.2025.1610134","url":null,"abstract":"","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1610134"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12079034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of different phytogenic-based additives on <i>in vitro</i> rumen fermentation profile and methane emissions.","authors":"Hani M El-Zaiat, Hussein A Masood, Samira S Al Hinai, Reem H Al Maamari, Sanad S Al Riyami, Kaadhia Al-Kharousi, Azza H Al-Salami, Nasser Al-Habsi","doi":"10.3389/fvets.2025.1591700","DOIUrl":"https://doi.org/10.3389/fvets.2025.1591700","url":null,"abstract":"<p><strong>Introduction: </strong>Phytogenic feed additives have gained increasing attention in ruminant nutrition due to their capacity to modulate ruminal fermentation and reduce methane (CH₄) emissions. This study evaluated the effects of three plant-based additives.</p><p><strong>Methods: </strong>Neem leaf (<i>Azadirachta indica</i>; NL), Indigofera leaf (<i>Indigofera oblongifolia</i>; IL), and Pumpkin peel (<i>Cucurbita pepo</i>; PP) included at four levels (0, 10, 20, and 30 g/kg DM) on <i>in vitro</i> ruminal fermentation, feed degradability, and CH₄ emissions. A total mixed ration (TMR) was incubated with each additive and buffered rumen fluid using the <i>in vitro</i> gas production technique for 24 hours.</p><p><strong>Results and discussion: </strong>NL and IL supplementation significantly (<i>p</i> < 0.05) increased gas and carbon dioxide (CO₂) production, organic matter degradability, and total volatile fatty acid concentrations, particularly propionate and butyrate. Simultaneously, acetate concentration, CH₄ emissions, NH₃-N levels, and protozoa abundance were reduced (<i>p</i> < 0.05). However, PP had limited effects on these parameters. The phenolic profiles of NL and IL, notably rich in 2-hydroxycinnamic acid and p-coumaric acid, likely contributed to these outcomes. In conclusion, these findings support the use of NL and IL as effective phytogenic additives for improving rumen fermentation and mitigating CH₄ production. Further <i>in vivo</i> trials are recommended to validate these <i>in vitro</i> results.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1591700"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrical impedance tomography in calves with bovine respiratory disease: correlations with clinical and blood gas findings.","authors":"Ulrich Bleul, Fabienne Kluser, Andreas Waldmann, Christian Gerspach","doi":"10.3389/fvets.2025.1556943","DOIUrl":"https://doi.org/10.3389/fvets.2025.1556943","url":null,"abstract":"<p><p>Bovine respiratory disease (BRD) is a multifactorial global problem associated with long-term deleterious effects on the well-being of calves and marked financial losses. Prompt diagnosis of BRD, monitoring the success of treatment, and providing an accurate prognosis remain challenging because current methods for stall-side diagnosis are inadequate. To improve diagnosis in addition to clinical and morphological findings and gain insight into the respiratory dynamics of BRD, thoracic electrical impedance tomography (EIT) was used to evaluate calves with BRD (Group D; <i>n</i> = 42) and healthy calves (Group H; <i>n</i> = 13). Thoracic EIT is a non-invasive method of quantifying differences in impedance changes between various lung regions and impedance changes over time. A belt with 32 equidistantly mounted electrodes was placed around the thorax of non-sedated calves of both groups to measure impedance changes during respiration. The results were compared with the clinical findings and the California BRD scores. Compared with group H, Group D had decreased ventilation in the ventral lung regions (<i>p</i> = 0.05); ventilation shifted to the left lung lobes in calves with marked auscultatory changes (<i>p</i> = 0.013). In addition, the quartile ventilation ratio on inspiration (V_QRi), used to quantify changes in impedance during inspiration, differed significantly between the two groups (<i>p</i> = 0.0039). Of all the EIT parameters, V_QRi correlated most closely with paO₂ and the A-a-gradient and was significantly lower in group D than in group H (<i>p</i> = 0.061). The results of EIT revealed differences in the inspiratory dynamics of clinically healthy and ill calves and correlated with the clinical and blood gas findings. Thus, EIT can be used alone or together with other diagnostic tools to identify and monitor BRD in calves.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1556943"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive mitochondrial genomics of <i>Fasciola gigantica</i> from Sudan: insights into genetic diversity, evolutionary dynamics, and host adaptation.","authors":"Bashir Salim, Nouh S Mohamed, Kamal Ibrahim, Saeed Alasmari, Elisha Chatanga, Yuma Ohari, Nariaki Nonaka, Mohammad A Alsaad, Faisal Almathen, Ryo Nakao","doi":"10.3389/fvets.2025.1577469","DOIUrl":"https://doi.org/10.3389/fvets.2025.1577469","url":null,"abstract":"<p><strong>Introduction: </strong>This study presents a comprehensive analysis of the complete mitochondrial genomes of <i>Fasciola gigantica</i> isolated from cattle, sheep, and goats in Sudan, aiming to provide new insights into genetic diversity, evolutionary dynamics, and host adaptation.</p><p><strong>Methods: </strong>Mitochondrial genomes were sequenced using high-throughput Illumina MiSeq technology, yielding sequences of 14,483 bp, slightly longer than the reference genome (14,478 bp). A sliding window analysis was conducted to assess nucleotide diversity, and phylogenetic analyses were performed using complete mitochondrial sequences, including and excluding non-coding regions.</p><p><strong>Results: </strong>Key genetic variations were observed, including a non-canonical start codon (GTG) in the ND5 gene and an alternative stop codon (TAA) in ND4. Length polymorphisms in ND4L and <i>cox1</i> suggested potential mitochondrial efficiency adaptations. Non-coding regions showed minor length differences, with the long non-coding region extending by 20 bp and the short by 4 bp. Sliding window analysis identified ND4 and ND5 as the most variable genes, while <i>cox1</i>, <i>nd1,</i> <i>and</i> <i>cox2</i> were the most conserved. Phylogenetic analysis showed distinct clustering of Sudanese <i>F. gigantica</i> isolates with strong bootstrap support. Excluding the D-loop preserved phylogenetic structure, while D-loop-specific analysis revealed high variability, particularly in the sheep isolate.</p><p><strong>Discussion: </strong>These findings highlight significant genetic variation and evolutionary divergence among <i>F. gigantica</i> isolates in Sudan. The observed diversity, particularly within non-coding and variable coding regions, underscores the influence of regional evolutionary pressures and host-associated adaptations. This work enhances understanding of <i>F. gigantica</i>'s genetic landscape and supports the development of more targeted molecular surveillance and control strategies for fascioliasis in endemic regions.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1577469"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Life expectancy and survival analysis for companion dogs and cats in Seoul, South Korea.","authors":"Isaac Yang, Dae-Sung Yoo, Kyung-Duk Min","doi":"10.3389/fvets.2025.1532422","DOIUrl":"https://doi.org/10.3389/fvets.2025.1532422","url":null,"abstract":"<p><strong>Background: </strong>Investigating life expectancy and mortality is crucial for the development of evidence-based health strategies for companion animals. However, relevant studies are lacking in South Korea, possibly because of challenges in collecting mortality data. In this regard, preliminary analyses were conducted to obtain life tables for companion animals in South Korea.</p><p><strong>Methods: </strong>The electronic records of six veterinary hospitals in Seoul, South Korea were examined. The data collected included breed, sex, spay/neuter status, date of birth, and date of death for all dogs and cats with a verifiable date of death since November 1, 2004 until December 31, 2022. After data preprocessing, descriptive statistical analysis was performed to summarize the demographics, and life tables and survival curves were created for dogs and cats. Cox proportional hazards regression was used to analyze the effects of demographic factors on survival.</p><p><strong>Results: </strong>The mean age of dogs at death was 3427.49 days. Spayed or neutered dogs had a significantly higher life expectancy than intact dogs. Mixed-breed dogs had a higher life expectancy than purebred dogs. For cats, the mean age at death was 1965.49 days, with spayed or neutered cats living significantly longer than intact cats. Purebred cats had a higher median survival than Mixed-breed cats. Spaying or neutering and breed significantly affected survival probabilities in both species.</p><p><strong>Conclusion: </strong>Our study provides insights into the longevity of companion animals in South Korea, and reveals that neutering and breed significantly influence life expectancy.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1532422"},"PeriodicalIF":2.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lnc MSTRG 4701.7 targets miR-1786/RORa to competitively regulate proliferation and apoptosis in chicken follicular granulosa cells.","authors":"Chang Ma, Hengsong Wu, Chunchi Yan, Simushi Liswaniso, Xue Sun, Ning Qin, Rifu Xu","doi":"10.3389/fvets.2025.1583287","DOIUrl":"https://doi.org/10.3389/fvets.2025.1583287","url":null,"abstract":"<p><strong>Background: </strong>Follicular development in chickens is a highly coordinated and complex process. While the hypothalamic-pituitary-gonadal axis plays a central regulatory role in this process, the role of long noncoding RNAs (LncRNAs) remains unclear. Here, we aimed to identify key differentially expressed LncRNAs and explore their regulatory roles in follicular development, particularly focusing on the MSTRG.4701.7-miR-1786-RORa axis, to provide insights for improving fertility in low-fertility chicken varieties.</p><p><strong>Methods: </strong>Intact follicular tissues were collected from Jilin Black chickens (low-fertility group) and Lohmann Brown Laying hens (high-fertility group) at each stage of ovarian development for transcriptome sequencing to identify key differentially expressed LncRNAs for follow-up analyses. Bioinformatics analysis was also performed to determine the role of the MSTRG.4701.7-miR-1786-RORa regulatory axis, and to clarify whether that MSTRG 4701.7 targets miR-1786/RORa to competitively regulate the proliferation and differentiation of chicken follicular granulosa cells (GCs). We also knocked down and overexpressed key genes, MSTRG.4701.7 and miR-1786 and thereafter, determined changes in the mRNA and protein expression levels of axial terminal mRNA-RORa.</p><p><strong>Results: </strong>MSTRG.4701.7 promotes the apoptosis of chicken follicular GCs, while miR-1786 reverses this phenomenon. RORa was also identified as a functional target of miR-1786 in GCs, and as a competitive endogenous RNA, MSTRG.4701.7 regulated RORa expression by sponging miR-1786, thereby playing a regulatory role in GCs. Additionally, interfering with MSTRG.4701.7 expression significantly downregulated RORa mRNA and protein expression levels, while interfering with miR-1786 showed the opposite effect.</p><p><strong>Conclusion: </strong>MSTRG.4701.7 plays a critical role in follicular development in chickens by influencing GC proliferation, differentiation, and apoptosis. The resent results provide useful molecular evidence for elucidating the genetic mechanism underlying ovarian follicle development associated with egg production in chicken.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1583287"},"PeriodicalIF":2.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel NKG2A alpaca nanobody targeting immune checkpoint blockade for the treatment of malignant melanoma.","authors":"Xiang Guo, Congfang Guo, Dongxiao Li, Yuting Bai, Mureed Abbas, Ruiwen Fan, Yiyan Zhao","doi":"10.3389/fvets.2025.1571857","DOIUrl":"https://doi.org/10.3389/fvets.2025.1571857","url":null,"abstract":"<p><p>Alpacas belong to the Camelidae family. Antibodies produced through alpaca immunization are called nanoantibodies. Compared to traditional antibodies, nanoantibodies have several characteristics, including smaller molecular weight, stable structure, high homology with human antibodies, and suitability for prokaryotic expression. Malignant melanoma (MM) is a severe and aggressive form of cancer that affects both humans and animals. It commonly arises in the mucous membranes of the skin, nose, mouth, anus, and digestive tract, as well as in the choroid of the eyes. Multiple factors contribute to melanocyte carcinogenesis, including ultraviolet (UV) radiation, endocrine disorders, viral infections, immune deficiencies, and chemical carcinogens. At present, surgical resection remains to be the primary treatment for MM, although the prognosis is generally poor. However, targeted therapy and immune checkpoint inhibitors (ICIs) are increasingly employed in the clinical treatment of melanoma. NKG2A is an inhibitory receptor protein found on the surface of CD8⁺ T cells and natural killer (NK) cells. HLA-E ligands expressed on the surface of malignant melanoma cells can facilitate immune evasion by binding to the NKG2A receptor complex on immune cells. This interaction suppresses immune responses, enabling tumor cells to escape immune surveillance. Immunosuppressive antibody drugs work by blocking this recognition mechanism, thereby reactivating immune cells to target and destroy tumor cells. As such, NKG2A has emerged as a novel target for immunotherapeutic intervention. In this study, an alpaca-derived nanoantibody targeting NKG2A with high affinity was obtained from a melanoma-specific phage library display. Through induced expression and protein purification, a monoclonal nanobody strain expressing NKG2A was successfully isolated. This NKG2A-targeting nanobody demonstrates the potential for application in both the detection and treatment of MM.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1571857"},"PeriodicalIF":2.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case Report: Use of veterinary cuttable plates to maintain reduction and reinforce polymethylmethacrylate (PMMA) fixation in two canine C2 vertebral fractures.","authors":"Olivia M Snead, Andy Y Law, Megan R MacRae, Hannah R Yoder, Karl H Kraus","doi":"10.3389/fvets.2025.1534966","DOIUrl":"https://doi.org/10.3389/fvets.2025.1534966","url":null,"abstract":"<p><p>Veterinary cuttable plates (VCPs) were used to maintain reduction and provide reinforcement in screw and polymethylmethacrylate (PMMA) repairs of C2 vertebral fractures in two dogs. A 6-month-old male intact 3.8 kg Shih Tzu and a 4-year-old male intact 30.5 kg Golden Retriever presented after motor vehicle accidents. Computed tomography (CT) scans revealed fractures of the C2 vertebrae in both dogs. Fractures were reduced and stabilized with a combination of cortical screws, VCPs, and PMMA. The novel technique utilized veterinary cuttable plates positioned between screws cranial and caudal to the fracture for distraction and reduction of the fractures and can be incorporated into the acrylic. Post-operative imaging showed adequate fracture reduction and relief of spinal cord compression. Recheck examinations demonstrated a return to normal and near-normal neurologic function in both patients.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1534966"},"PeriodicalIF":2.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}