Frontiers in Veterinary Science最新文献

{"title":"Ultrasonographic examination of the patellar ligament after capsular and fascial imbrication for the treatment of cranial cruciate ligament rupture in dogs.","authors":"Mario Candela Andrade, Senta Maria Hoffmann, Pavel Slunsky, Ignacio De Rus Aznar, Leo Brunnberg","doi":"10.3389/fvets.2025.1544445","DOIUrl":"https://doi.org/10.3389/fvets.2025.1544445","url":null,"abstract":"<p><strong>Introduction: </strong>Cranial cruciate ligament (CCL) rupture is the most common orthopedic condition in dogs, with many surgical options available for its treatment. Thickening of the patellar ligament after capsular and fascial imbrication (CFI) is a frequently reported complication, but its clinical significance remains unclear.</p><p><strong>Method: </strong>This prospective study evaluated patellar ligament structural and thickness changes after CCL repair using CFI. Forty-six dogs with CCL ruptures treated at the Small Animal Clinic of Freie Universität Berlin between July 2013 and April 2015 were included. Clinical, radiographic, and sonographic assessments were performed pre-surgery and at 2-3 months and 8-10 months post-surgery. Parameters assessed included lameness scores, joint effusion, stability, extension pain, muscular atrophy, and arthritis. Uninjured contralateral joints from 20 dogs served as controls.</p><p><strong>Results: </strong>Postoperative improvements included reduced lameness scores, joint effusion, instability, and extension pain. Patellar ligament thickness increased from 1.6 mm pre-surgery to 5.4 mm at 2-3 months, then decreased to 3.9 mm by 8-10 months. Structural changes peaked at the first follow-up (52.9%) and decreased by the second (6.4%). No significant correlations were found between ligament changes and clinical outcomes, patient demographics, or adjunct treatments like NSAIDs or physical therapy.</p><p><strong>Conclusion: </strong>Patellar ligament alterations following CFI appear temporary and largely unrelated to clinical signs, patient factors, or treatment variables. These findings suggest that such changes do not significantly impact postoperative outcomes, underscoring the reliability of CFI as a treatment option for CCL rupture in dogs.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1544445"},"PeriodicalIF":2.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11977418/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiviral activity of <i>Scutellaria baicalensis Georgi Extract</i> against Getah virus <i>in vivo</i> and <i>in vitro</i>.","authors":"Baoling Liu, Yuling Wang, Lina Shao, Yuanhang Chen, Zhiwen Xu, Ling Zhu","doi":"10.3389/fvets.2025.1551501","DOIUrl":"https://doi.org/10.3389/fvets.2025.1551501","url":null,"abstract":"<p><strong>Introduction: </strong>The Getah virus (GETV) is a zoonotic arbovirus causing disease in humans and animals, a member of the <i>Alphavirus</i> genus. Currently, approved antiviral drugs and vaccines against alphaviruses are few available. This study aimed to investigate the anti-GETV activity of the Extract of <i>Scutellaria baicalensis Georgi</i> (ESG) <i>in vivo</i> and <i>in vitro</i>.</p><p><strong>Methods: </strong>The cytotoxic effects of ESG on BHK-21 cells were quantitatively evaluated through the MTT assay. Quantitative analysis of viral replication was performed using qRT-PCR, while E2 protein expression was analyzed through western blotting. Furthermore, molecular docking simulations were conducted to examine the binding affinity between the principal bioactive constituents of ESG and the E2 structural proteins. Additionally, the therapeutic potential of ESG in alleviating viremia was evaluated in GETV-infected mouse models.</p><p><strong>Results: </strong>The results showed that ESG significantly attenuated the cytopathic effects induced by GETV infection in BHK-21 cells, concurrently reducing both viral replication and E2 protein expression. Notably, ESG exhibited its most potent antiviral activity during the viral attachment and entry phases, with IC50 values of 3.69 μg/mL and 3.94 μg/mL, respectively. At a concentration of 10 μg/mL, ESG achieved 95.08% inhibition efficiency against viral attachment. Furthermore, in vivo studies revealed that ESG treatment significantly reduced the peak viral load and shortened the duration of viremia in GETV-infected mice. The main components of ESG are baicalin and baicalein, and molecular docking simulations demonstrated strong binding affinities between these compounds and the active site of GETV E2 protein, with docking scores of -6.99 kcal/mol for baicalin and -5.21 kcal/mol for baicalein.</p><p><strong>Conclusion: </strong>The experimental findings demonstrate that ESG exhibits significant antiviral efficacy against GETV infection both <i>in vitro</i> and <i>in vivo</i>. These results indicate that ESG represents a promising therapeutic candidate for the prevention and treatment of GETV infections. Mechanistically, the antiviral activity of ESG appears to be mediated, at least in part, through the modulation of E2 protein expression.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1551501"},"PeriodicalIF":2.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11977417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lumbosacral (myelo) meningoceles in dogs, related tethered cord syndrome, and their surgical management: review of the literature and clinical experience.","authors":"Patrick Roynard, Curtis Wells Dewey","doi":"10.3389/fvets.2025.1510800","DOIUrl":"https://doi.org/10.3389/fvets.2025.1510800","url":null,"abstract":"<p><p>Neural tube defects (NTDs) are a group of congenital malformations characterized by various levels of protrusions of meninges with or without nervous tissue through incomplete osseous coverage (cranium bifidum for the cranial forms and spina bifida for spinal meningoceles/myelomeningoceles [MCs/MMCs]), with associated dorsal midline cutaneous signs. Amongst a confusing vocabulary, spina bifida is both the term most used to refer to NTDs and the most common manifestation of NTDs, with a predilection for the lumbosacral area in screw-tail breeds. With the growing popularity of bulldogs, lumbosacral (LS) MCs/MMCs are increasingly encountered, and small animal practitioners should learn to recognize them. Clinical signs may include urinary and/or fecal incontinence, pelvic limb neurological deficits with bunny hopping (neurolocalization L4-caudal or subset), and cutaneous signs (swirl of hair and dimple); the combination of which is pathognomonic of these disorders in bulldog puppies. Since these malformations often trigger a tethered cord syndrome (TCS), neurological worsening is possible. While historically reported to be somewhat hopeless regarding neurological improvement, isolated case reports, small case series, and personal experience of the author indicates that post-operative improvement is possible. Review of the literature (14 cases) and personal surgical experience (9 cases) retrieved 23 canine cases of LS MC/MMC treated surgically with follow-up. Clinical presentation, diagnostic imaging findings (CT and MRI), and intra- and post-operative findings are discussed in this article, along with a detailed description of the surgical technique. Pelvic limb deficits improve post-surgically in most cases (14/17 [82%] cases with pre-operative deficits and follow-up ≥1 month) albeit sometimes only marginally. Urinary/fecal continence can improve also, although less frequently (10/21 [48%] at 1 month follow-up and 8/21 [38%] at ≥6 months).</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1510800"},"PeriodicalIF":2.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11975860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors associated with the use of important human antimicrobials in Japanese small-animal clinics.","authors":"Kohei Makita, Mao Yoshida, Makoto Ukita, Takeshi Matsuoka, Masato Sakai, Yutaka Tamura","doi":"10.3389/fvets.2025.1496422","DOIUrl":"https://doi.org/10.3389/fvets.2025.1496422","url":null,"abstract":"<p><strong>Introduction: </strong>In Japan, programs to monitor antimicrobial use in companion animals have not been established. To fill this gap, the Japan Veterinary Medical Association has conducted surveys of actual use. The aims of this paper are to clarify the frequency and factors associated with the use of human antimicrobials in Japanese small-animal clinics.</p><p><strong>Methods: </strong>Antimicrobial usage and awareness surveys were conducted at 260 veterinary clinics between November 2021 and February 2022 using two questionnaires. The annual use of each antimicrobial drug was categorized by frequency, and the median value of each category, with a score of 50 for the choice ≥50, was used to quantify usage frequency. Important antimicrobial drugs for human use were defined as rank I antibiotics of the Food Safety Commission. Knowledge and awareness factors associated with the use of important antimicrobial drugs for human use were analyzed using three approaches. First, the use of important drugs was examined using a generalized linear model (GLM) with binomial errors. Second, a vector generalized linear and additive model with zero-inflated binomial errors was used to evaluate the proportion of important drugs among the annual frequency of use of human antimicrobial drugs. Third, at the drug level, selecting veterinary clinics using important human drugs, univariable GLMs with Poisson errors were used to evaluate the frequency of important human drug use, with the log number of employees as the offset term.</p><p><strong>Results: </strong>The response rates were 71.2 and 72.3% for the antimicrobial usage and awareness surveys, respectively. All of the facilities used human antimicrobial drugs, and 57.1% (93/163) of facilities used important human antimicrobial drugs. Important human antimicrobial drugs accounted for 21.7% of the frequency of use of human antimicrobial drugs annually (7,342/33,896 times). In terms of the proportion of important human drugs and frequency of important human drug use, the use of important human antimicrobial drugs was low in cases of high awareness of prudent use of antimicrobial drugs and where tests for evidence-based judgment were introduced, but was high when price and ease of use were emphasized.</p><p><strong>Discussion: </strong>Antibiotic stewardship should be further promoted in Japanese small-animal clinics through educational and information dissemination activities.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1496422"},"PeriodicalIF":2.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11975915/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-typhoidal <i>Salmonella</i> in food animals in Paraguay: predominant serovars and resistance phenotypes.","authors":"Rossana Irrazábal, María V Iriarte, Julio Alvarez","doi":"10.3389/fvets.2025.1521469","DOIUrl":"https://doi.org/10.3389/fvets.2025.1521469","url":null,"abstract":"<p><p>Surveillance of antimicrobial resistance (AMR) in <i>Salmonella</i> in livestock (poultry, pig, and cattle) is crucial to maintain food safety. Given the lack of information on the situation in livestock in Paraguay, the aim of this study was to determine the most frequent <i>Salmonella</i> serovars in poultry, pig and cattle sampled in slaughterhouses in the country in 2020-22 along with their AMR phenotypes using data from a national pilot program. Out of 1,161 samples collected from slaughtered animals originating from 189 farms nationwide, <i>Salmonella</i> was isolated from 91/384 (23.7%) samples from poultry, 52/390 (13.3%) from pigs and 6/387 (1.6%) from cattle. Seven serovars were identified in poultry, with Heidelberg being the most frequent (82.4% of 91 isolates), while the most frequent serovars in pigs were Panama (48.1%) and Typhimurium (38.5%), and only two serovars (Cerro and Braenderup) were identified in cattle. The proportion of resistant isolates ranged from extremely high (70-83% for nalidixic acid and tetracycline) and high (25-40% for nitrofurantoin and ampicilin) to low-moderate (8-18% for cefixime, cefotaxime, amoxicillin, and trimethoprim- sulfamethoxazole) and very low-low (<6% for ciprofloxacin and gentamicin) depending on the antimicrobial. Up to 23 different resistance profiles were found, ranging from pansusceptible (18/143 isolates) to resistance to 2-7 antimicrobials (median = 2), with the predominant serovars in poultry and swine typically being resistant to ≥3 antimicrobials. These results should be backed-up with genomic analyses to determine the genetic mechanisms involved in the resistance profiles observed in order to support coordinated actions for AMR surveillance and control in the country.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1521469"},"PeriodicalIF":2.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11977416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of canine distemper virus in wildlife in Italy (2022-2024).","authors":"Flora Alfano, Maria Gabriella Lucibelli, Nicola D'Alessio, Clementina Auriemma, Simona Rea, Giovanni Sgroi, Maria Stella Lucente, Francesco Pellegrini, Georgia Diakoudi, Esterina De Carlo, Nicola Decaro, Gianvito Lanave, Vito Martella, Giovanna Fusco","doi":"10.3389/fvets.2025.1527550","DOIUrl":"https://doi.org/10.3389/fvets.2025.1527550","url":null,"abstract":"<p><p>CDV has been detected in a wide range of domestic and wild animal also in Italy and it is highly prone to cross-species transmission, therefore representing a significant health risk. In this study the presence of CDV and other coinfecting selected viruses, in wild carnivorans of the family Mustelidae and Canidae and rodents of the family Hystricidae, collected in Southern Italy (Campania region), in 2022-2024, was investigated. Over a period of 3 years (2022-2024), tissue samples from 136 wild animals including stone martens, porcupines, otters, wolves, martens, badgers and foxes were examined. CDV RNA was detected in 14 (10.3%) animals encompassing badgers (<i>n</i> = 6), foxes (<i>n</i> = 5), wolves (<i>n</i> = 2), and marten (<i>n</i> = 1). The complete genome of a CDV strain was reconstructed from a spleen sample of a badger. On sequence and phylogenetic analyses, the novel CDV strain belonged to the Arctic clade, which has already been reported from badger and dog in Italy. Our study contributes to extend the knowledge on the epidemiology of CDV in wildlife and confirm the need for a continuous surveillance in wild animals to monitor the circulation in wildlife of viruses pathogenic for domestic carnivores and endangered wild species.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1527550"},"PeriodicalIF":2.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11975880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Animal health and nutrition: metabolic disorders in cattle and improvement strategies.","authors":"Danju Kang, Shera Elizabeth Lungu, Felix Danso, Chrystella Fernanda Dzou, Yanjun Chen, Xinyu Zheng, Fanghong Nie, Hongying Lin, Jinjun Chen, Guangxian Zhou","doi":"10.3389/fvets.2025.1470391","DOIUrl":"https://doi.org/10.3389/fvets.2025.1470391","url":null,"abstract":"<p><p>The health and productivity of cattle are significantly compromised by metabolic diseases on a global scale. These disorders disrupt normal metabolic processes, leading to substantial economic losses for the livestock industry. Metabolic disorders can arise from defective biochemical pathways, deficiencies in enzymes, coenzymes, or cofactors, and may be either inherited or acquired. Dairy cows are particularly susceptible during the transition period from late lactation to early management, facing conditions such as ketosis, milk fever, and hepatic lipidosis. This susceptibility is primarily due to reduced dry matter intake caused by fetal development and a decline in rumen capacity. The negative energy balance (NEB) during this phase, characterized by elevated blood concentrations of non-esterified fatty acids (NEFAs) due to increased energy mobilization, is closely linked to the onset of these diseases. Providing high-energy-density diets during this period is critical to mitigating the effects of NEB. Metabolic disorders represent a major health challenge in cattle, adversely affecting animal welfare and agricultural output. A comprehensive understanding of their etiology, clinical manifestations, diagnostic approaches, and management strategies is essential for effective prevention and treatment. Ongoing research and the adoption of preventive measures are vital to reducing the economic and health impacts of these diseases. Early diagnosis and proactive management strategies are crucial to mitigating their impact on dairy cattle health and productivity. Early identification enables timely interventions, preventing disease progression and reducing adverse effects on animal health. Proactive measures, such as optimizing nutritional programs, implementing precision farming technologies, and ensuring timely veterinary care, are essential for enhancing the overall wellbeing of dairy cows. This review serves as a valuable resource for veterinarians, researchers, and dairy farmers, offering in-depth insights into the etiology, clinical signs, diagnostics, and management of prevalent metabolic disorders in dairy cattle. By equipping stakeholders with this knowledge, it aims to support informed decision-making and improve herd management practices. The focus on early diagnosis and proactive strategies underscores the potential to significantly reduce the economic and health burdens imposed by metabolic diseases on the livestock industry.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1470391"},"PeriodicalIF":2.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11977490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum cortisol level as marker of stress in camels: relationship with immunological profile.","authors":"Jamal Hussen, Hind Althagafi","doi":"10.3389/fvets.2025.1570564","DOIUrl":"https://doi.org/10.3389/fvets.2025.1570564","url":null,"abstract":"<p><p>The present study evaluated serum cortisol levels as a stress indicator in camels and analyzed the influence of some physiological and pathological factors on cortisol levels and their relationship with immunological parameters. A total number of 169 camels (<i>Camelus dromedarius</i>) were grouped in a healthy group (<i>n</i> = 106 camels), a slaughterhouse group (<i>n</i> = 20 camels), a Surra-affected group (<i>n</i> = 27 camels), and a metritis-affected group (<i>n</i> = 16 camels). Female camels exhibited higher cortisol levels compared to males, with non-pregnant and lactating she-camels showing elevated levels relative to their pregnant and non-lactating counterparts. No significant differences in cortisol levels were observed among camel breeds (Majaheem, Magateer, Sawahli, and Omani). Elevated cortisol levels were observed in stressed camels (pre-slaughter) and diseased camels, including those with Surra and bacterial metritis, confirming the reliability of cortisol as a stress marker in this species. Increased cortisol levels were associated with leukocytosis, neutrophilia, and a higher neutrophil-to-lymphocyte ratio. Phenotypically, elevated cortisol level was associated with an expanded CD4 T-cell population, reduced γδ T cells percentage, decreased CD172a expression on neutrophils and monocytes, reduced CD14 and CD163 expression on monocytes, and enhanced CD45 and MHC I expression on lymphocytes. Functionally, higher cortisol levels were linked to increased reactive oxygen species (ROS) production in blood phagocytes. These findings highlight the modulatory effects of cortisol on the camel immune system and emphasize the importance of considering gender and reproductive status when evaluating stress in camels.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1570564"},"PeriodicalIF":2.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11975896/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A picture of purpose: exploring veterinary students' motivations through photo elicitation.","authors":"Ronald J Orchard, Cassidy Moreau, Brad Crauer","doi":"10.3389/fvets.2025.1553433","DOIUrl":"10.3389/fvets.2025.1553433","url":null,"abstract":"<p><p>Understanding the motivations that drive veterinary students is essential for supporting their academic success, wellbeing, and professional satisfaction. This qualitative study utilized photo elicitation, a visual and narrative research method, to explore the personal, emotional, and professional \"whys\" of 14 veterinary students pursuing a Doctor of Veterinary Medicine (DVM) degree. Participants submitted photographs and reflective narratives describing their motivations and how these motivations supported their journeys. Thematic analysis revealed five key themes: (1) Deep Emotional Bonds with Animals, (2) Overcoming Personal Adversity, (3) The Influence of Role Models, (4) Desire to Make a Difference, and (5) Community and Service. These findings highlight the central role of formative experiences, relationships, and values in shaping students' aspirations and perseverance. The study underscores the potential for reflective practices, such as photo elicitation, to reconnect students with their intrinsic motivations, fostering resilience and long-term satisfaction in veterinary practice. Integrating principles such as compassionate advocacy, spectrum of care, and community engagement into veterinary education may better align training with students' values, enhancing their preparation for impactful and fulfilling careers.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1553433"},"PeriodicalIF":2.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11974251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferritinophagy: a possible new iron-related metabolic target in canine osteoblastic osteosarcoma.","authors":"Karen Power, Rebecca Leandri, Giorgia Federico, Gionata De Vico, Leonardo Leonardi","doi":"10.3389/fvets.2025.1546872","DOIUrl":"10.3389/fvets.2025.1546872","url":null,"abstract":"<p><p>Canine osteosarcomas (COS) are the most common bone tumors in dogs, characterized by high metastatic rates, poor prognosis, and poor responsiveness to routine therapies, which highlights the need for new treatment targets. In this context, the metabolism of neoplastic cells represents an increasingly studied element, as cancer cells depend on particular metabolic pathways that are also elements of vulnerability. Among these, tumor cells (TCs) show higher iron requirements to sustain proliferation (so-called iron addiction), which are achieved by increasing iron uptake and/or by activating ferritinophagy, a process mediated by the Nuclear receptor Co-Activator 4 (NCOA4) leading to iron mobilization from ferritin (Ft) deposits. Previous studies have shown that COS cells overexpress Transferrin Receptor 1 (TfR1) to increase iron uptake. In this study we evaluated the immunohistochemical expression of ferritinophagy-related proteins, namely Ferritin Heavy chain (FTH1) and NCOA4, and proliferating cell nuclear antigen (PCNA) in canine normal bone and canine osteoblastic osteosarcoma (COOS) samples. Normal samples revealed negative/weak immunoreactivity for FTH1, NCOA4 and PCNA in <10% of osteocytes. In COOS samples the majority of neoplastic cells showed immunoreactivity to FTH1, NCOA4 and PCNA. Our data suggest that the activation of ferritinophagy by COOS cells responds to the need for feed their \"iron addiction.\" These data, though preliminary, further suggest that targeting iron metabolism represents a new potential strategy worthy of further study to be transferred into clinical practice.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1546872"},"PeriodicalIF":2.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}