Frontiers in Veterinary Science最新文献

{"title":"Case report: <i>In vivo</i> detection of neutrophil extracellular traps in a dog with thrombosis induced by bacterial vasculitis.","authors":"Ju-Yun Kim, Hyun-Jung Han","doi":"10.3389/fvets.2025.1470605","DOIUrl":"10.3389/fvets.2025.1470605","url":null,"abstract":"<p><p>This case report describes NETosis as a cause of thrombosis in an 18.3 kg, 8-year-old intact male mixed-breed dog with bacterial vasculitis. The dog presented with sudden paresis of the thoracic limb, characterized by cyanosis, absent arterial pulse, and decreased peripheral blood glucose levels. Doppler ultrasound confirmed thrombosis in the dorsal common digital artery. Histopathology post-amputation revealed bacterial vasculitis, thrombosis, and infarction, with immunohistochemical staining identifying extracellular citrullinated histone H3 (CitH3), indicative of NETs involvement. Treatment included antibiotics, pentoxifylline, and anticoagulants, showing transient improvement before disease progression and euthanasia due to respiratory signs. These findings suggest NETs as a potential therapeutic target for bacterial vasculitis in similar cases.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1470605"},"PeriodicalIF":2.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11862914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a reverse genetics system for an epidemic strain of porcine rotavirus JXAY01 type G5P[23]I12.","authors":"Changjin Liu, Huangsiwu Wei, Xingyi Zhang, Wenjie Wu, Zhengqiao Shen, Feng Luo, Shunzhou Deng","doi":"10.3389/fvets.2024.1512327","DOIUrl":"10.3389/fvets.2024.1512327","url":null,"abstract":"<p><p>Porcine rotavirus is one of the most important pathogens causing diarrhea in newborn piglets, and the genome of this virus contains 11 double-stranded RNA segments, which are easy to be recombined among strains to produce new strains with different antigenic properties. The reverse genetics system is an informative tool for studying virus biology. Recently, adaptable plasmid-based reverse genetics systems were developed for the porcine rotavirus OSU strain; however, such systems have not been developed for epidemic porcine rotavirus genotypes in China. In this study, we successfully established a reverse genetic system based on an epidemic strain of porcine rotavirus JXAY01 isolated in recent years, which was characterized by a specific genotype constellation: G5-P[23]-I12-R1-C1-M1-A8-N1-T7-E1-H1. 11 gene segments of porcine rotavirus JXAY01 were cloned into plasmid vectors similar to the SA11 system. JXAY01 genome segment plasmids were co-transfected with 10 complementary SA11 genome plasmids, and 11 monoreassortant strains were successfully rescued. Viral replication analyses of the parental SA11 strain and the monoreassortant strains showed that the structural protein replacement monoreassortants had reduced cell proliferation compared with the parental SA11 and non-structural protein replacement strains. The recombinant rJXAY01 strain could be successfully rescued using 11 pRG-JXAY01 plasmids. Whole genome sequencing showed 12 amino acid differences between the isolate JXAY01 and the recombinant rJXAY01, but there was no significant difference in their <i>in vitro</i> replication ability. This study reports the reverse genetic system, which lays the foundation for further understanding of porcine rotavirus molecular biology and novel vaccine development.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"11 ","pages":"1512327"},"PeriodicalIF":2.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11862993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case report: Reconstruction with thyrohyoidopexy in a dog presented with complete laryngo-tracheal separation.","authors":"José Diogo Dos-Santos, Luís Belo, Mariana Monteiro, Lisa A Mestrinho","doi":"10.3389/fvets.2025.1482151","DOIUrl":"10.3389/fvets.2025.1482151","url":null,"abstract":"<p><p>A 2-year-old Norfolk Terrier purebred female dog was presented for urgent attention after strangulation secondary to elevator entrapment. The traumatic event caused a complex laryngopharyngeal lesion with total laryngotracheal and esophageal separation from the hyoid bone and pharynx. Reconstruction was performed from the posterior pharyngeal wall, and all layers, mucosa, muscles and ligaments were repaired. A thyrohyoidopexy was done using nonabsorbable sutures to reinforce the thyrohyoid membrane reconstruction and prevent reseparation in the immediate postoperative period. The patient's fully recovery was gradual but uneventful, with occasional cough resolving within 2 months.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1482151"},"PeriodicalIF":2.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11863270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of anemoside B4 in canine pneumonia treatment: a prospective, randomized controlled trial.","authors":"Jinzhao Ji, Xiaoqing Ding, Chuanli Liu, Lingling Dai, Junting Yu, Linghao Li, Shaobing Wan, Yangyang Song, Junqing Zhao, Zhetong Su, Kun Jia, Shoujun Li","doi":"10.3389/fvets.2025.1530318","DOIUrl":"10.3389/fvets.2025.1530318","url":null,"abstract":"<p><strong>Background: </strong>Canine pneumonia is a serious respiratory disease often associated with Canine Infectious Respiratory Disease (CIRD). Current treatment strategies primarily rely on antibiotics and corticosteroids; however, the emergence of antibiotic resistance and potential side effects from prolonged corticosteroid use limit the effectiveness of these therapies in clinical practice. These challenges highlight the urgent need for alternative treatments. Anemoside B4 (AB4), derived from the traditional Chinese medicine <i>Pulsatilla</i>, has shown promise in preclinical studies for modulating inflammatory responses and improving clinical symptoms of pneumonia. Therefore, AB4 may offer a valuable alternative treatment option for canine pneumonia in veterinary medicine.</p><p><strong>Methods: </strong>A prospective, randomized controlled trial was conducted at the Veterinary Drug Research and Evaluation Center of South China Agricultural University. Seventy-two dogs with mild-to-moderate pneumonia were randomly assigned to one of three groups: AB4, placebo, or Chuanxinlian injection (CXL). The primary outcome was the effect of AB4 on comprehensive clinical scoring of canine pneumonia; secondary outcomes included recovery times for primary symptoms and efficacy assessments. Additionally, AB4's safety in clinical applications was evaluated.</p><p><strong>Results: </strong>The AB4 group demonstrated significantly lower composite clinical scores on Days 7 and 14 compared to the placebo group (<i>p</i> = 0.033 and <i>p</i> = 0.000, respectively). Significant differences in recovery times for fever and dyspnea were observed between the AB4 and placebo groups (<i>p</i> = 0.041 and <i>p</i> = 0.024, respectively). Moreover, the cure rate and overall efficacy on Day 14 were significantly higher in the AB4 group than those in the placebo group (<i>p</i> = 0.001 and <i>p</i> = 0.009, respectively).</p><p><strong>Conclusion: </strong>These findings suggest that AB4 may be a promising treatment option for canine pneumonia, potentially serving as an alternative to traditional therapies. Further research is needed to explore its clinical potential in veterinary medicine.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1530318"},"PeriodicalIF":2.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11861352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding barriers to veterinary involvement in dairy calf health management.","authors":"Kristen Y Edwards, Angel Abuelo, Stephen J LeBlanc, Trevor J DeVries, Michael A Steele, Joao H C Costa, David L Renaud","doi":"10.3389/fvets.2025.1503915","DOIUrl":"10.3389/fvets.2025.1503915","url":null,"abstract":"<p><p>The objectives of this cross-sectional study were to identify barriers to veterinary involvement in calf health and assess knowledge gaps in calf care among American and Canadian bovine veterinarians. A questionnaire was administered to veterinarians, collecting data on demographics, satisfaction with calf health management knowledge, involvement in decision-making, satisfaction with calf health involvement, frequency of calf health record analysis and feedback, topics of interest for further learning, and preferred learning formats. Multivariable logistic regression models were used to assess associations between variables and outcomes. Only 28% of veterinarians frequently reviewed calf health records, and 44% made actionable recommendations after reviewing them. Female veterinarians were more likely than male veterinarians to frequently review calf health records (Odds ratio - OR: 2.9, 95% CI: 1.2-7.3). Additionally, the odds of frequently reviewing records increased with the amount of time spent working with calves (OR: 10.2 per 10% increment, 95% CI: 10.0-10.5). Veterinarians highly satisfied with their knowledge of neonatal calf diarrhea (NCD) prevention were more likely to make recommendations based on records (OR: 11.6, 95% CI: 1.9-72.4). Additionally, those frequently reviewing records were more likely to provide feedback (OR: 15.5, 95% CI: 4.0-60.3). Incomplete records was the most common reason for not reviewing records (60% of respondents) and why actionable recommendations were made less frequently than \"most of the time\" (67% of respondents). Veterinarians were least confident in their knowledge regarding milk feeding and weaning recommendations but they were interested in learning more about post-weaning nutrition and automated calf feeders. Further, they preferred conference presentations for continuing education. These findings suggest that veterinary involvement in calf health could be improved by facilitating better data capture and enhancing veterinarian knowledge.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1503915"},"PeriodicalIF":2.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11861529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of point-of-care quantitative serum canine pancreatic lipase testing for diagnosing acute pancreatitis in dogs.","authors":"Pin-Chen Liu, Kendy Tzu-Yun Teng, Tsia-Lu Lin, Chi-Hsuan Sung, Tsun-Li Cheng, Chi-Chung Chou","doi":"10.3389/fvets.2025.1421103","DOIUrl":"10.3389/fvets.2025.1421103","url":null,"abstract":"<p><strong>Introduction: </strong>Current point-of-care testing for canine-specific pancreatic lipase (CPL) provides semi-quantitative measurements with binary results. Recently, a commercial point-of-care testing method (Vcheck CPL) that offers quantitative measurement of CPL has emerged. However, clinical studies on its value (or utility) are limited. Therefore, this study aimed to evaluate the clinical utility of this commercial point-of-care CPL in diagnosing dogs with suspected acute pancreatitis and to assess its correlation with a commercial semi-quantitative test and other clinicopathological variables.</p><p><strong>Methods: </strong>A prospective observational study included 33 dogs with suspected acute pancreatitis and 20 clinically healthy dogs. Serum Vcheck CPL and SNAP ® cPL were tested, and clinical consensus scores were determined by 5 internists. Eleven dogs with suspected acute pancreatitis underwent follow-up testing during hospitalization. The intra-class correlation coefficient (ICC) was used for statistical analysis to assess the agreement between assays and the internists' consensus score.</p><p><strong>Results: </strong>Dogs with suspected acute pancreatitis had significantly higher serum Vcheck CPL (median: 843 μg/L, range: 77-2001, <i>p</i> < 0.0001) than healthy control dogs (median: 94 μg/L, range: 49-294). By day 3 of hospitalization, serum Vcheck CPL had significantly decreased in dogs with suspected acute pancreatitis compared to day 1. The ICC score between the clinical consensus score, Vcheck CPL, and SNAP ® cPL was 0.75, indicating good agreement. Serum Vcheck CPL concentration was significantly correlated with serum concentrations of amylase, lipase, creatinine, ALP, and CRP.</p><p><strong>Discussion: </strong>This study found good agreement between Vcheck CPL and SNAP ® cPL. This quantitative Vcheck CPL testing could serve as an adjunctive tool in diagnosing dogs with acute pancreatitis.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1421103"},"PeriodicalIF":2.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11860965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Climate-smart livestock nutrition in semi-arid Southern African agricultural systems.","authors":"Felix Fushai, Teedzai Chitura, Oyegunle Emmanuel Oke","doi":"10.3389/fvets.2025.1507152","DOIUrl":"10.3389/fvets.2025.1507152","url":null,"abstract":"<p><p>Climate change is disrupting the semi-arid agricultural systems in Southern Africa, where livestock is crucial to food security and livelihoods. This review evaluates the bioenergetic and agroecological scope for climate-adaptive livestock nutrition in the region. An analysis of the literature on climate change implications on livestock nutrition and thermal welfare in the regional agroecological context was conducted. The information gathered was systematically synthesized into tabular summaries of the fundamentals of climate-smart bioenergetics, thermoregulation, livestock heat stress defence mechanisms, the thermo-bioactive feed components, and potentially climate-smart feed resources in the region. The analysis supports the adoption of climate-smart livestock nutrition when conceptualized as precision feeding combined with dietary strategies that enhance thermal resilience in livestock, and the adaptation of production systems to the decline in availability of conventional feedstuffs by incorporating climate-smart alternatives. The keystone potential climate-smart alternative feedstuffs are identified to be the small cereal grains, such as sorghum (<i>Sorghum bicolor</i>) and pearl millet (<i>Pennisetum glaucum</i>) as dietary energy sources, the native legumes, such as the cowpea (<i>Vigna unguiculata</i>) and the marama bean (<i>Tylosema esculentum</i>) as protein sources, wild browse Fabaceae trees such as <i>Vachellia</i> spp. and <i>Colophospermum mopane</i>, which provide dry season and drought supplementary protein, minerals, and antioxidants, the non-fabaceous tree species such as the marula tree (<i>Sclerocarya birrea</i>), from which animals consume the energy and electrolyte-rich fresh fruit or processed pulp. Feedstuffs for potential circular feeding systems include the oilseed cakes from the macadamia (<i>Macadamia integrifolia</i>) nut, the castor (<i>Ricinus communis</i>), and Jatropha (<i>Jatropha curcas</i>) beans, which are rich in protein and energy, insect feed protein and energy, primarily the black soldier fly larvae (<i>Hermetia illucens</i>), and microbial protein from phototrophic algae (<i>Spirulina</i>, <i>Chlorella</i>), and yeasts (<i>Saccharomyces cerevisiae</i>). Additives for thermo-functionally enhanced diets include synthetic and natural anti-oxidants, phytogenics, biotic agents (prebiotics, probiotics, synbiotics, postbiotics), and electrolytes. The review presents a conceptual framework for climate-smart feeding strategies that enhance system resilience across the livestock-energy-water-food nexus, to inform broader, in-depth research, promote climate-smart farm practices and support governmental policies which are tailored to the agroecology of the region.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1507152"},"PeriodicalIF":2.6,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11851964/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring mental health in veterinary students: common stressors and effective coping strategies: a narrative review.","authors":"Rahib K Islam, Emily K Cobb, Hannah K McCowan, Kylie Watson, Kaustuv Bhattacharya, Anjali Chandra, Warda Mohiuddin, Karen Gruszynski, Amanda H Wilkerson, John J Dascanio, Robert E Davis, Vinayak K Nahar","doi":"10.3389/fvets.2025.1470022","DOIUrl":"10.3389/fvets.2025.1470022","url":null,"abstract":"<p><strong>Introduction: </strong>Veterinary students face significant challenges impacting their mental health and wellbeing. The rigorous academic curriculum, high expectations, and demanding clinical training create an environment of intense pressure and constant stress. This review explores the demographic and psychosocial variables influencing mental health outcomes, highlighting common stressors and coping strategies.</p><p><strong>Methods: </strong>A systematic literature search was conducted using PubMed, CAB Abstracts, and Google Scholar. Studies published up to 2021 involving veterinary students were included. Twenty-one peer-reviewed studies met the inclusion criteria. Demographic data, stressors, mental health measures, and coping strategies were extracted and analyzed. Studies focused on various mental health aspects, including stress, anxiety, depression, and coping mechanisms. The final selection was based on relevance, quality, and comprehensiveness.</p><p><strong>Results: </strong>The reviewed studies indicated that rigorous academic demands, high expectations, and clinical training contribute to elevated stress levels among veterinary students. Women reported higher levels of stress, anxiety, and depression compared to men, necessitating gender-specific interventions. Social support and relationship quality were crucial for mental wellbeing, with students in supportive environments experiencing lower stress. Effective coping strategies included exercise and social activities; whereas, harmful behaviors like substance misuse exacerbated mental health issues. Despite using mental health services, barriers such as stigma and lack of awareness hindered access for some students.</p><p><strong>Conclusion: </strong>The findings underscore the need for targeted support systems to enhance the mental health and resilience of veterinary students. Interventions to promote healthy coping mechanisms, reduce stigma, and improve access to mental health resources are essential. Veterinary schools should prioritize creating a supportive environment to ensure students can manage the demands of their profession while maintaining their mental health and wellbeing.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1470022"},"PeriodicalIF":2.6,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11866424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-term effects of argon cold atmospheric plasma on canine corneas <i>ex vivo</i>.","authors":"Juliane Dick, Sandra Lockow, Wolfgang Baumgärtner, Holger Andreas Volk, Claudia Busse","doi":"10.3389/fvets.2025.1518071","DOIUrl":"10.3389/fvets.2025.1518071","url":null,"abstract":"<p><strong>Purpose: </strong>To analyse the effects of argon cold atmospheric plasma (CAP) on canine corneas.</p><p><strong>Methods: </strong>Healthy canine eyes (<i>n</i> = 20) were subjected to a keratectomy (5 × 7 mm, 400 μm) and divided into two control (c1-not cultured; t0-cultured) and two treatment groups (t2, t5-treatment of 2 or 5 min, cultured); <i>n</i> = 5 eyes each. The kINPen^® VET (neoplas GmbH, Greifswald, Germany) was used for CAP treatment. Corneas (t0, t2, t5) were cultured at an air-liquid interface (72 h). Histopathological and immunohistochemical (Ki-67, Caspase-3, α-SMA) examinations were performed.</p><p><strong>Results: </strong>Corneal epithelization was complete and epithelial thickness was similar in all eyes. The number of perilimbal epithelial cell nuclei varied between groups with c1 = 22 ± 6, t0 = 13 ± 5, t2 = 15 ± 5 and t5 = 10 ± 4 nuclei/randomized fields and was lowest in t5, which was significantly different from t2 but not from t0. Ki-67 positive cells in the stroma varied between groups with c1 = 0.2 ± 0.45, t0 = 8 ± 12, t2 = 18 ± 12 and t5 = 10 ± 7 positive cells/section. More Ki-67 positive cells were found in t2 compared to t5. This was not significantly different from t0. Caspase-3 and α-SMA expression were similar in all treatment groups.</p><p><strong>Conclusion: </strong>Canine corneas treated with CAP showed similar corneal wound healing compared to untreated corneas <i>ex vivo</i>. A 5-min CAP application results in a lower perilimbal epithelial cell density and fewer Ki67 positive stromal cells compared to the 2-min treatment.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1518071"},"PeriodicalIF":2.6,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11851940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The case for development of a core outcome set (COS) and supplemental reporting guidelines for influenza vaccine challenge trial research in swine.","authors":"Sheila Keay, Famke Alberts, Annette M O'Connor, Robert Friendship, Terri O'Sullivan, Zvonimir Poljak","doi":"10.3389/fvets.2025.1465926","DOIUrl":"10.3389/fvets.2025.1465926","url":null,"abstract":"<p><p>Previously, we systematically reviewed more than 20 years of influenza vaccine challenge trial research in pigs to answer the question, \"does vaccinating sows protect offspring?\" Overall, most studies were well designed but clinical heterogeneity made between-study comparisons challenging. Studies varied by samples, outcomes, and assays selected for measurement. Additionally, data essential for inclusion of findings in meta-analyses were often insufficiently reported and as a result, summary effect measures were either not derived or were not meaningful. Clinical heterogeneity and reporting issues complicate and limit what can be learned cumulatively from research and both represent two types of avoidable research waste. Here, we illustrate each concern using data collected tangentially during the systematic review and propose two corrective strategies, both of which have broad applicability across veterinary intervention research; (i) develop a Core Outcome Set (COS) to reduce unnecessary clinical heterogeneity in future research and (ii) encourage funders and journal editors to require submitted research protocols and manuscripts adhere to established reporting guidelines. As a reporting corollary, we developed a supplemental checklist specific to influenza vaccine challenge trial research in swine and propose that it is completed by researchers and included with all study protocol and manuscript submissions. The checklist serves two purposes: as a reminder of details essential to report for inclusion of findings in meta-analyses and sub-group meta-analyses (e.g., antigenic or genomic descriptions of influenza vaccine and challenge viruses), and as an aid to help synthesis researchers fully characterize and comprehensively include studies in reviews.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1465926"},"PeriodicalIF":2.6,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11851948/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}