Can Kong, Dingsheng Kong, Junjie Hu, Lianggong Liao, Zhiguo Xiong, Tao Fu
{"title":"小鼠手术后肠内和肠外营养模型。","authors":"Can Kong, Dingsheng Kong, Junjie Hu, Lianggong Liao, Zhiguo Xiong, Tao Fu","doi":"10.3389/fvets.2025.1626574","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>This study aims to establish a mouse model with different nutritional support modalities [enteral nutrition (EN) and parenteral nutrition] after abdominal surgery, providing a stable and effective animal model for further study on the intestinal barrier damage caused by total parenteral nutrition.</p><p><strong>Methods: </strong>Twenty mice were randomly assigned to either an enteral nutrition (EN) group (<i>n</i> = 10) or a total parenteral nutrition (TPN) group (<i>n</i> = 10). After abdominal surgery via appendectomy, the two groups of mice received different modes of nutritional support (TPN or EN). A properly sized central venous catheter was placed in the right internal jugular or an enteral nutrition catheter was inserted into the duodenum. Parenteral or enteral nutrition was infused through these catheters. The survival rate, intestinal villus height, epithelial cell proliferation, changes in goblet cells and Paneth cells, as well as the expression levels of the tight junction (TJ) proteins ZO-1 and occludin, were observed.</p><p><strong>Results: </strong>No difference was found in survival rate (<i>P</i> < 0.01) between the two groups. However, significant differences were observed in the height of small intestinal villi, epithelial proliferation rate, number of goblet cells [Periodic Acid-Schiff (PAS+)], Paneth cell function (Lysozyme+), and expression of ZO-1 and occludin proteins. All of these markers were significantly decreased in the TPN group compared to the EN group (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>The mouse model is suitable and effective for investigating the pathogenesis of intestinal barrier dysfunction, as it provides different types of nutritional support after abdominal surgery.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1626574"},"PeriodicalIF":2.9000,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492947/pdf/","citationCount":"0","resultStr":"{\"title\":\"Mouse models for enteral and parenteral nutrition after surgery.\",\"authors\":\"Can Kong, Dingsheng Kong, Junjie Hu, Lianggong Liao, Zhiguo Xiong, Tao Fu\",\"doi\":\"10.3389/fvets.2025.1626574\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>This study aims to establish a mouse model with different nutritional support modalities [enteral nutrition (EN) and parenteral nutrition] after abdominal surgery, providing a stable and effective animal model for further study on the intestinal barrier damage caused by total parenteral nutrition.</p><p><strong>Methods: </strong>Twenty mice were randomly assigned to either an enteral nutrition (EN) group (<i>n</i> = 10) or a total parenteral nutrition (TPN) group (<i>n</i> = 10). After abdominal surgery via appendectomy, the two groups of mice received different modes of nutritional support (TPN or EN). A properly sized central venous catheter was placed in the right internal jugular or an enteral nutrition catheter was inserted into the duodenum. Parenteral or enteral nutrition was infused through these catheters. The survival rate, intestinal villus height, epithelial cell proliferation, changes in goblet cells and Paneth cells, as well as the expression levels of the tight junction (TJ) proteins ZO-1 and occludin, were observed.</p><p><strong>Results: </strong>No difference was found in survival rate (<i>P</i> < 0.01) between the two groups. However, significant differences were observed in the height of small intestinal villi, epithelial proliferation rate, number of goblet cells [Periodic Acid-Schiff (PAS+)], Paneth cell function (Lysozyme+), and expression of ZO-1 and occludin proteins. All of these markers were significantly decreased in the TPN group compared to the EN group (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>The mouse model is suitable and effective for investigating the pathogenesis of intestinal barrier dysfunction, as it provides different types of nutritional support after abdominal surgery.</p>\",\"PeriodicalId\":12772,\"journal\":{\"name\":\"Frontiers in Veterinary Science\",\"volume\":\"12 \",\"pages\":\"1626574\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-09-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492947/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Veterinary Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.3389/fvets.2025.1626574\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Veterinary Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3389/fvets.2025.1626574","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Mouse models for enteral and parenteral nutrition after surgery.
Background: This study aims to establish a mouse model with different nutritional support modalities [enteral nutrition (EN) and parenteral nutrition] after abdominal surgery, providing a stable and effective animal model for further study on the intestinal barrier damage caused by total parenteral nutrition.
Methods: Twenty mice were randomly assigned to either an enteral nutrition (EN) group (n = 10) or a total parenteral nutrition (TPN) group (n = 10). After abdominal surgery via appendectomy, the two groups of mice received different modes of nutritional support (TPN or EN). A properly sized central venous catheter was placed in the right internal jugular or an enteral nutrition catheter was inserted into the duodenum. Parenteral or enteral nutrition was infused through these catheters. The survival rate, intestinal villus height, epithelial cell proliferation, changes in goblet cells and Paneth cells, as well as the expression levels of the tight junction (TJ) proteins ZO-1 and occludin, were observed.
Results: No difference was found in survival rate (P < 0.01) between the two groups. However, significant differences were observed in the height of small intestinal villi, epithelial proliferation rate, number of goblet cells [Periodic Acid-Schiff (PAS+)], Paneth cell function (Lysozyme+), and expression of ZO-1 and occludin proteins. All of these markers were significantly decreased in the TPN group compared to the EN group (P < 0.05).
Conclusion: The mouse model is suitable and effective for investigating the pathogenesis of intestinal barrier dysfunction, as it provides different types of nutritional support after abdominal surgery.
期刊介绍:
Frontiers in Veterinary Science is a global, peer-reviewed, Open Access journal that bridges animal and human health, brings a comparative approach to medical and surgical challenges, and advances innovative biotechnology and therapy.
Veterinary research today is interdisciplinary, collaborative, and socially relevant, transforming how we understand and investigate animal health and disease. Fundamental research in emerging infectious diseases, predictive genomics, stem cell therapy, and translational modelling is grounded within the integrative social context of public and environmental health, wildlife conservation, novel biomarkers, societal well-being, and cutting-edge clinical practice and specialization. Frontiers in Veterinary Science brings a 21st-century approach—networked, collaborative, and Open Access—to communicate this progress and innovation to both the specialist and to the wider audience of readers in the field.
Frontiers in Veterinary Science publishes articles on outstanding discoveries across a wide spectrum of translational, foundational, and clinical research. The journal''s mission is to bring all relevant veterinary sciences together on a single platform with the goal of improving animal and human health.