小鼠手术后肠内和肠外营养模型。

IF 2.9 2区 农林科学 Q1 VETERINARY SCIENCES
Frontiers in Veterinary Science Pub Date : 2025-09-19 eCollection Date: 2025-01-01 DOI:10.3389/fvets.2025.1626574
Can Kong, Dingsheng Kong, Junjie Hu, Lianggong Liao, Zhiguo Xiong, Tao Fu
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引用次数: 0

摘要

背景:本研究旨在建立腹部手术后不同营养支持方式[肠内营养(EN)和肠外营养]的小鼠模型,为进一步研究全肠外营养对肠道屏障的损害提供稳定有效的动物模型。方法:将20只小鼠随机分为肠内营养组(n = 10)和全肠外营养组(n = 10)。经阑尾切除腹部手术后,两组小鼠分别给予不同方式的营养支持(TPN或EN)。在右侧颈内静脉内置入适当大小的中心静脉导管或在十二指肠内置入肠内营养导管。通过这些导管注入肠外或肠内营养。观察成活率、肠绒毛高度、上皮细胞增殖、杯状细胞和Paneth细胞的变化以及紧密连接蛋白ZO-1和occludin的表达水平。结果:两组患者生存率差异无统计学意义(P < 0.01)。而在小肠绒毛高度、上皮细胞增殖率、杯状细胞(PAS+)数量、Paneth细胞功能(溶菌酶+)、ZO-1和occludin蛋白表达等方面存在显著差异。与EN组相比,TPN组上述指标均显著降低(P < 0.05)。结论:该小鼠模型可提供腹部手术后不同类型的营养支持,适用于研究肠屏障功能障碍的发病机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mouse models for enteral and parenteral nutrition after surgery.

Background: This study aims to establish a mouse model with different nutritional support modalities [enteral nutrition (EN) and parenteral nutrition] after abdominal surgery, providing a stable and effective animal model for further study on the intestinal barrier damage caused by total parenteral nutrition.

Methods: Twenty mice were randomly assigned to either an enteral nutrition (EN) group (n = 10) or a total parenteral nutrition (TPN) group (n = 10). After abdominal surgery via appendectomy, the two groups of mice received different modes of nutritional support (TPN or EN). A properly sized central venous catheter was placed in the right internal jugular or an enteral nutrition catheter was inserted into the duodenum. Parenteral or enteral nutrition was infused through these catheters. The survival rate, intestinal villus height, epithelial cell proliferation, changes in goblet cells and Paneth cells, as well as the expression levels of the tight junction (TJ) proteins ZO-1 and occludin, were observed.

Results: No difference was found in survival rate (P < 0.01) between the two groups. However, significant differences were observed in the height of small intestinal villi, epithelial proliferation rate, number of goblet cells [Periodic Acid-Schiff (PAS+)], Paneth cell function (Lysozyme+), and expression of ZO-1 and occludin proteins. All of these markers were significantly decreased in the TPN group compared to the EN group (P < 0.05).

Conclusion: The mouse model is suitable and effective for investigating the pathogenesis of intestinal barrier dysfunction, as it provides different types of nutritional support after abdominal surgery.

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来源期刊
Frontiers in Veterinary Science
Frontiers in Veterinary Science Veterinary-General Veterinary
CiteScore
4.80
自引率
9.40%
发文量
1870
审稿时长
14 weeks
期刊介绍: Frontiers in Veterinary Science is a global, peer-reviewed, Open Access journal that bridges animal and human health, brings a comparative approach to medical and surgical challenges, and advances innovative biotechnology and therapy. Veterinary research today is interdisciplinary, collaborative, and socially relevant, transforming how we understand and investigate animal health and disease. Fundamental research in emerging infectious diseases, predictive genomics, stem cell therapy, and translational modelling is grounded within the integrative social context of public and environmental health, wildlife conservation, novel biomarkers, societal well-being, and cutting-edge clinical practice and specialization. Frontiers in Veterinary Science brings a 21st-century approach—networked, collaborative, and Open Access—to communicate this progress and innovation to both the specialist and to the wider audience of readers in the field. Frontiers in Veterinary Science publishes articles on outstanding discoveries across a wide spectrum of translational, foundational, and clinical research. The journal''s mission is to bring all relevant veterinary sciences together on a single platform with the goal of improving animal and human health.
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