猪饲料中喷雾干燥猪血浆传播非洲猪瘟的风险评估以及紫外线照射作为附加安全措施的效果

IF 2.9 2区 农林科学 Q1 VETERINARY SCIENCES
Frontiers in Veterinary Science Pub Date : 2025-09-19 eCollection Date: 2025-01-01 DOI:10.3389/fvets.2025.1463720
Clazien J de Vos, Lourens Heres, Aldo Dekker, Manon Swanenburg, W Meindert Pelser, Jacob Post, Marcel M Hulst
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引用次数: 0

摘要

世界范围内非洲猪瘟(ASF)疫情的增加引起了对猪喷雾干猪血浆(SDPP)喂养的关注。因此,将血液加工成SDPP应能保证充分灭活非洲猪瘟病毒(ASFV),从而生产出安全的产品。本研究的目的是评估(i)将感染非洲猪瘟的猪的血液加工成SDPP并喂给仔猪所需的灭活水平,以及(ii)如果在喷雾干燥前对血浆进行紫外线处理,则可获得额外的安全性。建立定量微生物风险评估(QMRA)模型,评估饲喂从单个非洲猪瘟感染猪群采集的血液中提取的SDPP的断奶仔猪感染概率(Pinf)。使用实验室规模的移动“冷巴氏杀菌”装置(Lyras inc .,奥尔堡,丹麦)对紫外线灭活ASFV进行定量处理。用不同剂量的UV-C辐照猪血浆,并计算其log10还原因子(LRF)。最高UV-C照射剂量(~137 焦耳/m2)的平均LRF为2.2。QMRA模型结果表明,在处理过程中,LRF必须达到5,才能达到pif < 0.01的中位数,即每100个asf感染批次中导致新感染的批次少于1个。LRF为8时,pif的第95百分位值也< 0.01。这些结果与报道的喷雾干燥和干燥储存的LRF值进行了比较,后者在5.2和11.1之间变化。因此,将UV- c照射作为SDPP生产的额外步骤提供了额外的安全保证,因为喷雾干燥、干燥储存和UV处理的联合失活水平可能导致总体LRF≥8,这意味着新ASF感染的风险非常低(中位数Pinf为7.3 × 10-6;95百分位为1.6 × 10-3)。QMRA模型没有考虑到非洲猪瘟感染猪无意中被加工成SDPP的可能性。如果SDPP不是来自非洲猪瘟疫区的猪,则这种可能性很低,从而进一步降低了SDPP感染非洲猪瘟的风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Risk assessment of African swine fever transmission by spray-dried porcine plasma in piglet feed and the effect of UV irradiation treatment as an additional safety step.

The increase of African swine fever (ASF) outbreaks worldwide has raised concerns about the feeding of spray-dried porcine plasma (SDPP) to pigs. The processing of blood into SDPP should thus guarantee sufficient inactivation of ASF virus (ASFV) to render a safe product. The objective of this study was to evaluate (i) the required level of inactivation if blood of ASF-infected pigs would be processed into SDPP and fed to piglets, and (ii) the additional safety achieved if UV treatment is applied to plasma before spray-drying. A quantitative microbial risk assessment (QMRA) model was built to assess the infection probability (Pinf ) of weaned piglets fed with SDPP produced from blood collected from a single ASF-infected herd. The inactivation of ASFV by UV treatment was quantified using a mobile, laboratory-scale "Cold Pasteurization" apparatus (Lyras inc, Aalborg, Denmark). Porcine plasma spiked with blood collected from pigs experimentally infected with ASFV was irradiated with different doses of UV-C and the log10 reduction factor (LRF) calculated. An average LRF of 2.2 was achieved by the highest dose of UV-C irradiation applied (~137 Joule/m2). QMRA model results indicate that an LRF of 5 needs to be achieved during processing to arrive at a median value of Pinf < 0.01, i.e., less than 1 out of 100 ASF-infected batches resulting in new infections. With an LRF of 8, also the 95th percentile value of Pinf is < 0.01. These results were compared to reported LRF values of spray-drying and dry storage of SDPP, which varied between 5.2 and 11.1. Applying UV-C irradiation as an additional step in SDPP production thus provides extra safety guarantees as the combined inactivation levels of spray-drying, dry storage and UV treatment are likely to result in an overall LRF ≥ 8, implying a very low risk of new ASF infections (median Pinf 7.3 × 10-6; 95th percentile 1.6 × 10-3). The QMRA model did not account for the probability that ASF-infected pigs are unintendedly processed into SDPP. This probability is low if SDPP is not sourced from pigs in ASF-infected areas, therewith further reducing the ASF infection risk of SDPP.

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来源期刊
Frontiers in Veterinary Science
Frontiers in Veterinary Science Veterinary-General Veterinary
CiteScore
4.80
自引率
9.40%
发文量
1870
审稿时长
14 weeks
期刊介绍: Frontiers in Veterinary Science is a global, peer-reviewed, Open Access journal that bridges animal and human health, brings a comparative approach to medical and surgical challenges, and advances innovative biotechnology and therapy. Veterinary research today is interdisciplinary, collaborative, and socially relevant, transforming how we understand and investigate animal health and disease. Fundamental research in emerging infectious diseases, predictive genomics, stem cell therapy, and translational modelling is grounded within the integrative social context of public and environmental health, wildlife conservation, novel biomarkers, societal well-being, and cutting-edge clinical practice and specialization. Frontiers in Veterinary Science brings a 21st-century approach—networked, collaborative, and Open Access—to communicate this progress and innovation to both the specialist and to the wider audience of readers in the field. Frontiers in Veterinary Science publishes articles on outstanding discoveries across a wide spectrum of translational, foundational, and clinical research. The journal''s mission is to bring all relevant veterinary sciences together on a single platform with the goal of improving animal and human health.
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