Genetics and Molecular Biology最新文献_第2页

Virus-induced gene silencing as a tool for functional genomics in weeds: Challenges and future directions. 病毒诱导的基因沉默作为杂草功能基因组学的工具：挑战和未来方向。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-08-14 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0252

Érika Frydrych Capelari, Márcia Margis-Pinheiro, Aldo Merotto Junior, Rogerio Margis

{"title":"Virus-induced gene silencing as a tool for functional genomics in weeds: Challenges and future directions.","authors":"Érika Frydrych Capelari, Márcia Margis-Pinheiro, Aldo Merotto Junior, Rogerio Margis","doi":"10.1590/1678-4685-GMB-2025-0252","DOIUrl":"10.1590/1678-4685-GMB-2025-0252","url":null,"abstract":"Virus-induced gene silencing (VIGS) has evolved from a conceptual demonstration of antiviral defense into a pivotal reverse-genetics platform for plant functional genomics. By exploiting engineered DNA- or RNA-based viral vectors, VIGS enables rapid, sequence-specific transcript knockdown through RNA-mediated degradation of target transcripts. Recent refinements in vector design, inoculation strategies, and viral species selection, such as TRV, BSMV, and FoMV, have expanded its application to previously recalcitrant plants, including major crops and emerging weed models. In weeds, functional genomics remains particularly challenging due to high genetic variability, limited genomic resources, and incompatibility with conventional viral vectors and transformation systems. In this context, VIGS provides a tractable approach to investigate genes associated with herbicide resistance, metabolic adaptation, and stress tolerance. Beyond weed biology, its application to studies of immune signaling, hormonal crosstalk, and secondary metabolism highlights VIGS as a versatile biotechnology for elucidating gene function and supporting next-generation strategies in plant improvement and integrated pest management.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49Suppl 3 Suppl 3","pages":"e20250252"},"PeriodicalIF":1.7,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13544622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Root-enriched β-amylase GmBAM-like 1 enhances drought and salt tolerance in Arabidopsis. 富根β-淀粉酶GmBAM-like 1增强拟南芥耐旱性和耐盐性。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-08-14 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0234

Fábia Guimarães-Dias, Lucas Leal Lima, Anna Cristina Neves-Borges, João Travassos-Lins, Dulce Mantuano, Michel Georges Alberts Vincentz, Américo José Carvalho Viana, Márcio Alves-Ferreira

{"title":"Root-enriched β-amylase GmBAM-like 1 enhances drought and salt tolerance in Arabidopsis.","authors":"Fábia Guimarães-Dias, Lucas Leal Lima, Anna Cristina Neves-Borges, João Travassos-Lins, Dulce Mantuano, Michel Georges Alberts Vincentz, Américo José Carvalho Viana, Márcio Alves-Ferreira","doi":"10.1590/1678-4685-GMB-2025-0234","DOIUrl":"10.1590/1678-4685-GMB-2025-0234","url":null,"abstract":"Water scarcity impacts soybean cultivation and productivity globally. The ability of plants to withstand drought stress involves complex molecular and physiological mechanisms that facilitate the restoration and maintenance of cellular homeostasis. This study identified genes associated with carbohydrate metabolism and GABA shunt pathway that respond to water deficit in two soybean varieties. These varieties exhibited contrasting responses to water scarcity, and were subjected to two distinct cropping systems. In the drought-tolerant variety, a strategy for conferring tolerance was observed through the pre-emptive priming of the drought response. By applying multivariate analysis, we identified a pivotal gene, GmBAM-like 1, which responds to water scarcity. GmBAM-like 1 encodes a β-amylase and showed rapid activation and elevated expression levels in root tissues of the tolerant variety, suggesting its potential involvement in the drought tolerance response. Transgenic Arabidopsis plants overexpressing GmBAM-like 1 demonstrated enhanced tolerance to salt and osmotic stress, as evidenced by increased survival and germination rates. Additionally, after drought stress, these plants showed higher transpiration rates, larger leaf area, and greater relative water content upon rehydration. These findings demonstrate the potential of integrating the GmBAM-like 1 gene into plant breeding programs to develop cultivars with improved tolerance to water, salt, and osmotic stresses.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49Suppl 3 Suppl 3","pages":"e20250234"},"PeriodicalIF":1.7,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13544623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic susceptibility to mercury in the amazon: A cross-sectional approach to snps analyses in exposed riverine populations. 亚马逊河汞的遗传易感性：在暴露的河流种群中进行snps分析的横断面方法。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-08-14 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0115

Amanda Lopes-Araújo, Jean Ludger Barthelemy, Gabriela P Arrifano, Letícia Santos-Sacramento, Isabela Soares-Silva, Caio G Leal-Nazaré, Camila Lago-Pinheiro, Barbarella M Macchi, Rosa C Rodríguez Martín-Doimeadios, María Jiménez-Moreno, Marcus Augusto-Oliveira, Maria Elena Crespo-López

{"title":"Genetic susceptibility to mercury in the amazon: A cross-sectional approach to snps analyses in exposed riverine populations.","authors":"Amanda Lopes-Araújo, Jean Ludger Barthelemy, Gabriela P Arrifano, Letícia Santos-Sacramento, Isabela Soares-Silva, Caio G Leal-Nazaré, Camila Lago-Pinheiro, Barbarella M Macchi, Rosa C Rodríguez Martín-Doimeadios, María Jiménez-Moreno, Marcus Augusto-Oliveira, Maria Elena Crespo-López","doi":"10.1590/1678-4685-GMB-2025-0115","DOIUrl":"10.1590/1678-4685-GMB-2025-0115","url":null,"abstract":"Mercury is among the worst global pollutants being a significant public health concern in the Amazon, primarily due to contaminated fish intake. The relationship between mercury body burden and genetic polymorphisms in GSTP1 (rs1695), PON1 (rs662), MT1A (rs11076161), and SEPP1 (rs7579) genes was analyzed in Amazonian riverine populations, with a rapid and cost-effective approach by selecting individuals at both extremes of the exposure spectrum. Genotyping revealed significant associations for GSTP1 and PON1 polymorphisms: the G allele of GSTP1 and the CC genotype of PON1 were more frequent among highly exposed individuals, suggesting reduced detoxification efficiency. Additionally, although non-significant, a higher prevalence of the GG and CC genotypes were observed for MT1A and SEPP1, respectively, in the high-exposure group, pointing to the need for further investigation. These findings enhance our understanding of how genetic variation influences individual susceptibility to mercury accumulation in the Amazon and can support public health prevention strategies and the early identification of high-risk individuals within these vulnerable populations. Furthermore, this study underscores the importance of considering gene-environment interactions in environmental health assessments and highlights the need for public health strategies tailored to the genetic and socio-environmental contexts of vulnerable Amazonian communities.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49Suppl 4 Suppl 4","pages":"e20250115"},"PeriodicalIF":1.7,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13544621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coexpression network analysis of gastric adenocarcinoma identifies hub genes as biomarker candidates and their tumor microenvironment associations. 胃腺癌的共表达网络分析确定中心基因作为生物标志物候选人及其肿瘤微环境关联。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-08-10 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0119

Ronald Matheus da Silva Mourão, Fabiano Cordeiro Moreira, Jéssica Manoelli Costa da Silva, Daniel de Souza Avelar da Costa, Valéria Cristiane Santos da Silva, Amanda Ferreira Vidal, Leandro Lopes de Magalhães, Ana Karyssa Mendes Anaissi, Samia Demachki, Williams Fernandes Barra, Paulo Pimentel de Assumpção

{"title":"Coexpression network analysis of gastric adenocarcinoma identifies hub genes as biomarker candidates and their tumor microenvironment associations.","authors":"Ronald Matheus da Silva Mourão, Fabiano Cordeiro Moreira, Jéssica Manoelli Costa da Silva, Daniel de Souza Avelar da Costa, Valéria Cristiane Santos da Silva, Amanda Ferreira Vidal, Leandro Lopes de Magalhães, Ana Karyssa Mendes Anaissi, Samia Demachki, Williams Fernandes Barra, Paulo Pimentel de Assumpção","doi":"10.1590/1678-4685-GMB-2025-0119","DOIUrl":"https://doi.org/10.1590/1678-4685-GMB-2025-0119","url":null,"abstract":"Gastric adenocarcinoma (GAC) is characterized by molecular heterogeneity that limits early detection and targeted treatment. We applied weighted gene coexpression network analysis (WGCNA) to paired RNA-seq data from 119 GAC and peritumoral tissue (PTT) samples and identified six coexpression modules with distinct biological identities. Four modules were positively correlated with GAC and two were negatively correlated. Among the 30 hub genes evaluated, several outperformed established clinical biomarkers in accuracy. Specifically, SPARC (AUC = 0.89), COL3A1 (0.87), and COL1A2 (0.85) exceeded MUC5AC (0.76), VEGFA (0.68), and ERBB2 (0.64). Validation in the TCGA-STAD cohort confirmed concordant expression trends for MEblack (5/5 genes) and MEmagenta (4/5), with an overall fold-change correlation of ρ = 0.58 (p = 6.96 × 10⁻⁴). Immune deconvolution delineated two opposing microenvironmental axes, with an adaptive-immune-epithelial program (MEblack) associated with B-cell abundance, and a fibroblast-collagen program (MEmagenta) associated with cancer-associated fibroblast enrichment. DepMap CRISPR screening identified ribosomal hub genes as cell-intrinsic dependencies in gastric cancer cell lines. Among all hub genes, SPARC, COL3A1, COL1A2, GKN1, and GKN2 emerged as the potential biomarker candidates, with SPARC additionally showing a validated unfavorable prognostic association in STAD.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49Suppl 4 Suppl 4","pages":"e20250119"},"PeriodicalIF":1.7,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13474574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148764653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The shared performance of different COI regions increases the niche breadth detectable from bat guano eDNA. 不同COI区域的共同表现增加了从蝙蝠粪便eDNA中检测到的生态位宽度。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-08-07 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0080

Marcele Laux, Ana Cláudia Jardelino, Santelmo Vasconcelos, Eder Soares Pires, Enrico Bernard, Guilherme Oliveira, Caroline De-Souza, Gisele Lopes Nunes

{"title":"The shared performance of different COI regions increases the niche breadth detectable from bat guano eDNA.","authors":"Marcele Laux, Ana Cláudia Jardelino, Santelmo Vasconcelos, Eder Soares Pires, Enrico Bernard, Guilherme Oliveira, Caroline De-Souza, Gisele Lopes Nunes","doi":"10.1590/1678-4685-GMB-2025-0080","DOIUrl":"10.1590/1678-4685-GMB-2025-0080","url":null,"abstract":"The cytochrome c oxidase subunit I (COI), widely adopted as a DNA barcode for Metazoa, exhibits distinct variation levels in different regions within the gene. We investigated the efficacy of combining three arthropod-specific primers to enhance the detection of dietary niches, as opposed to relying on a single primer. This study utilized bat guano collected in bat caves from the Amazon and Caatinga biomes in Brazil. The UEA2-UEA3, UEA3-UEA4, and UEA5-UEA6 primer pairs recovered distinct proportions of Arthropoda (10%, 57%, and 42%, respectively) and exhibited considerable levels of unassigned reads (29%, 33%, and 42%, respectively) and non-target sequences (61%, 10%, and 16%, respectively). The UEA2-UEA3 primarily recovered Chiroptera (57%) but demonstrated the highest taxonomic coverage and richness for Arthropoda. On the other hand, UEA3-UEA4 and UEA5-UEA6 showed the highest α-diversity for Arthropoda; however, UEA5-UEA6 predominantly recovered Lepidoptera (40%) with the highest number of unique Arthropoda genera (45%), while UEA3-UEA4 mostly assigned to Lepidoptera and Diptera. Our results suggest the use of more than one primer pair and show that the analysis of only one primer pair can generate biased outputs. The choice primer is a crucial step in eDNA studies, especially for complex samples such as bat guano.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49 3","pages":"e20250080"},"PeriodicalIF":1.7,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13455176/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148701125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular and cytogenetic data for Rhamphichthys Müller and Troschel, 1848 (Rhamphichthyidae - Gymnotiformes): Evidence of chromosomal differentiation in R. heleios, R. hahni, R. rostratus and R. pantherinus. Rhamphichthys m<e:1> ller和Troschel， 1848 （Rhamphichthys Gymnotiformes）的分子和细胞遗传学数据：rr heleios， R. hahni， R. rostratus和R. pantherinus染色体分化的证据。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-08-03 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0092

Marlyson Jeremias Rodrigues da Costa, Patricia Correa da Silva, Cleusa Yoshiko Nagamachi, Wilsea Maria B Figueiredo Ready, Jonathan Stuart Ready, Julio Cesar Pieczarka

{"title":"Molecular and cytogenetic data for Rhamphichthys Müller and Troschel, 1848 (Rhamphichthyidae - Gymnotiformes): Evidence of chromosomal differentiation in R. heleios, R. hahni, R. rostratus and R. pantherinus.","authors":"Marlyson Jeremias Rodrigues da Costa, Patricia Correa da Silva, Cleusa Yoshiko Nagamachi, Wilsea Maria B Figueiredo Ready, Jonathan Stuart Ready, Julio Cesar Pieczarka","doi":"10.1590/1678-4685-GMB-2025-0092","DOIUrl":"10.1590/1678-4685-GMB-2025-0092","url":null,"abstract":"Rhamphichthys (Gymnotiformes), a genus with uncertain taxonomy due to rarity and morphological similarity, exhibits greater diversity in the Amazon Basin. Phylogenetic analysis of COI data from specimens collected in the Amazon Basin, in Guamá River (Belém and Barcarena), Caripetuba River, and Anequara River (Abaetetuba) and deposited in the collection of the Museu Paraense Emílio Goeldi identified two major monophyletic clades within Rhamphichthys, encompassing R. pantherinus and R. rostratus. Cytogenetic analysis of 24 specimens, both with 2n=50 but differing fundamental number (FN) and karyotype formula (KF), enabled their differentiation. Although 2n=50 is likely ancestral, karyotype constitution varies among species. By combining cytogenetic and molecular data, we reclassified the previously described Rhamphichthys \"marmoratus\" (2n=50, FN=94, KF=44m/sm+6st/a) as R. heleios, and previous R. rostratus (2n=50, FN=92, KF=42m/sm+8st/a) as R. pantherinus. This study provides the first cytogenetic data for the real R. rostratus species and reports novel B chromosomes within the Rhamphichthyidae family.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49Suppl 4 Suppl 4","pages":"e20250092"},"PeriodicalIF":1.7,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148688958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From few to many: How non-model organisms' genomics broadened our understanding of molecular evolution. 从少到多：非模式生物基因组学如何拓宽了我们对分子进化的理解。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-07-31 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0243

Mariana F Nery, Beatriz Daros, Ana Luiza Lein-Borba, Iasodara C L Santos, Beatriz F Leite, Luana Nara

{"title":"From few to many: How non-model organisms' genomics broadened our understanding of molecular evolution.","authors":"Mariana F Nery, Beatriz Daros, Ana Luiza Lein-Borba, Iasodara C L Santos, Beatriz F Leite, Luana Nara","doi":"10.1590/1678-4685-GMB-2025-0243","DOIUrl":"10.1590/1678-4685-GMB-2025-0243","url":null,"abstract":"For much of the 20th century, our understanding of genetics and evolution was predominantly shaped by intensive studies of a few model organisms such as Drosophila melanogaster, Caenorhabditis elegans, and Mus musculus. While these species provided fundamental insights, their laboratory-adapted characteristics potentially made them evolutionary outliers. The advent of next-generation sequencing technologies and sophisticated bioinformatic tools has increased accessibility of genomic research, enabling comprehensive studies of diverse non-model organisms across the tree of life. This expansion has substantially transformed our understanding of molecular evolution, revealing, for example, that convergent evolution operates through multiple mechanisms across different organizational levels, that speciation is a genomically heterogeneous process involving structural variants and adaptive introgression, and that genome architecture exhibits extensive variation in size, content, and organization. Using aquatic mammals as exemplars, we illustrate how comparative genomics of non-model species illuminates the molecular basis of convergent and divergent adaptations. This paradigm shift demonstrates that understanding evolution's general principles and creative solutions requires embracing life's full diversity.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49Suppl 1 Suppl 1","pages":"e20250243"},"PeriodicalIF":1.7,"publicationDate":"2026-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13426899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148648466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From individuals to communities: How genomics is transforming biodiversity conservation. 从个人到社区：基因组学如何改变生物多样性保护。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-07-27 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0329

Larissa S Arantes, Maycon Douglas de Oliveira, Henry Paul Granger-Neto, Zandora Celeste Hastenreiter, Rafael A Baggio, Barbara Regina Neves Chaves, Fabrício Rodrigues Santos

引用次数: 0

Expanding the mitochondrial genomic toolkit for Polyneoptera: New mitogenomes and evaluation of reduced marker sets for phylogeny and DNA barcoding. 扩展多翅目线粒体基因组工具包：新的有丝分裂基因组和评估系统发育和DNA条形码的减少标记集。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-07-24 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0282

Héctor Antônio Assunção Romão, Leonardo Carlos Jerônimo Corvalán, Juliana Alves Carneiro, David Daniel Ferreira Dos Santos, Rhewter Nunes, Renata de Oliveira Dias

{"title":"Expanding the mitochondrial genomic toolkit for Polyneoptera: New mitogenomes and evaluation of reduced marker sets for phylogeny and DNA barcoding.","authors":"Héctor Antônio Assunção Romão, Leonardo Carlos Jerônimo Corvalán, Juliana Alves Carneiro, David Daniel Ferreira Dos Santos, Rhewter Nunes, Renata de Oliveira Dias","doi":"10.1590/1678-4685-GMB-2025-0282","DOIUrl":"10.1590/1678-4685-GMB-2025-0282","url":null,"abstract":"Polyneoptera comprises hemimetabolous insect orders of significant agricultural, ecological, and medical relevance, motivating phylogenetic and molecular research that has nevertheless focused predominantly on canonical mitochondrial markers. Here, we assembled new mitogenomes for Polyneoptera and evaluated the usefulness of genes located in nucleotide-diversity hotspots as markers for species identification and phylogenetic inference. To expand the available mitogenomic resources, raw sequencing data were retrieved from public databases, resulting in the assembly and annotation of 26 complete mitogenomes, all exhibiting the typical insect mitochondrial architecture. These newly assembled genomes were combined with publicly available mitogenomes from Orthoptera, Blattodea, Plecoptera, Mantodea, and Phasmatodea to reconstruct phylogenetic relationships using both complete and reduced datasets comprising nucleotide-diversity hotspot-associated genes. The performance of these hotspot regions was further assessed through barcoding gap analyses and comparisons with the most comprehensive datasets to identify candidate mitochondrial markers for molecular species identification and phylogenetic inference. Across orders, different mitochondrial regions, including the classical markers 16S and COX1, as well as genes from the NADH dehydrogenase complex, emerged as the most informative, although optimal markers varied among lineages. Overall, our findings highlight the value of publicly accessible sequencing data for generating high-quality genomic resources and improving phylogenetic and taxonomic tools.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49 3","pages":"e20250282"},"PeriodicalIF":1.7,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13403772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148591200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of a major latex-like protein from wild Arachis (AdMLP11) confers tolerance to recurrent drought stress. 野生花生中一种主要的乳胶样蛋白AdMLP11的过度表达赋予其对反复干旱胁迫的耐受性。

IF 1.7 4区生物学

Genetics and Molecular Biology Pub Date : 2026-07-24 eCollection Date: 2026-01-01 DOI: 10.1590/1678-4685-GMB-2025-0151

Adrien Speck, Hugo Teixeira Gomes, Mario Alfredo Passos Saraiva, Patricia Messenberg Guimaraes, Ana Cristina Miranda Brasileiro

{"title":"Overexpression of a major latex-like protein from wild Arachis (AdMLP11) confers tolerance to recurrent drought stress.","authors":"Adrien Speck, Hugo Teixeira Gomes, Mario Alfredo Passos Saraiva, Patricia Messenberg Guimaraes, Ana Cristina Miranda Brasileiro","doi":"10.1590/1678-4685-GMB-2025-0151","DOIUrl":"10.1590/1678-4685-GMB-2025-0151","url":null,"abstract":"Recurrent drought episodes, increasingly intensified by climate change, pose a growing threat to global food security by severely limiting crop productivity. Major latex-like proteins (MLPs) play crucial roles in drought tolerance, acting as regulators of stress responses. However, their involvement in adaptation to recurrent drought stress remains poorly understood. In this study, we investigated the transcriptional dynamics of MLP genes during repeated dehydration and rehydration cycles in Arachis duranensis, a tropical wild species highly resilient to drought. In silico expression profiling of 36 A. duranensis MLP genes revealed their broad involvement in recurrent drought responses, with most patterns consistent with the 'revised-response' category of dehydration memory genes. qRT-PCR analysis further confirmed the activation of the abscisic acid (ABA) signaling pathway during recurrent drought in A. duranensis. Functional characterization of the candidate memory gene AdMLP11 in transgenic tobacco showed that its overexpression enhances tolerance to moderate and severe recurrent drought, likely through its role as a positive regulator of phytohormone-mediated defense pathways. These findings provide novel insights into the role of MLPs in transcriptional memory and drought adaptation in wild Arachis, highlighting AdMLP11 as a promising target for biotechnological strategies to develop climate-resilient crops.","PeriodicalId":12557,"journal":{"name":"Genetics and Molecular Biology","volume":"49 suppl 3","pages":"e20250151"},"PeriodicalIF":1.7,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13403773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148591169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0