Daniel Barzilay, João P B Alcino, Giulia M Ribeiro, Alfredo L P Sousa, Daniel J G Lahr
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引用次数: 0
摘要
长期以来,人们一直误认为变形虫拥有超大的基因组,在本文中,我们对这一误解的现有证据进行了重新评估。传统的估算依赖于不准确的方法,如 DNA 重量测量,这导致了夸大的大小。这些方法没有考虑到来自猎物、内共生体的污染 DNA 以及核糖体操作子扩增等固有基因组特征。现代测序技术揭示了一幅不同的图景。完全测序的变形虫基因组范围从 14.4 兆碱基对到 52.37 兆碱基对不等,完全符合典型单细胞真核生物的预期。虽然历史上相关的变形虫蛋白虫的全基因组尚未完全测序,但我们在此利用转录组数据中的蛋白编码基因进行了统计分析,结果表明其基因组大小与这一范围一致,远远小于之前的说法。这种误解很可能起源于 21 世纪初,并通过科普材料得以延续。我们的结论是,没有理由再重申阿米巴基因组是巨大的了。
Re-evaluating evidence for giant genomes in amoebae.
Here we reassess available evidence for the long-held misconception of amoebae possessing exceptionally large genomes. Traditionally, estimates relied on inaccurate methods like DNA weight measurements, leading to inflated sizes. These methods failed to account for contaminating DNA from prey, endosymbionts, and intrinsic genomic features like ribosomal operon amplification. Modern sequencing techniques unveil a different picture. Fully sequenced amoebozoa genomes range from 14.4 to 52.37 mega basepairs, well within the typical single-celled eukaryote expectation. While the whole genome of the historically relevant Amoeba proteus has not yet been fully sequenced, we provide here a statistical analysis using protein-coding genes from transcriptomic data, suggesting that the genome size is consistent with this range, far smaller than previously claimed. The misconception likely originated in the early 21st century and perpetuated through popular science materials. We conclude that there is no longer reason to reaffirm that amoeba genomes are giant.
期刊介绍:
Genetics and Molecular Biology (formerly named Revista Brasileira de Genética/Brazilian Journal of Genetics - ISSN 0100-8455) is published by the Sociedade Brasileira de Genética (Brazilian Society of Genetics).
The Journal considers contributions that present the results of original research in genetics, evolution and related scientific disciplines. Manuscripts presenting methods and applications only, without an analysis of genetic data, will not be considered.