巴西Yaravirus基因组结构分析及其对代谢的可能影响。

IF 1.7 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Genetics and Molecular Biology Pub Date : 2025-02-07 eCollection Date: 2025-01-01 DOI:10.1590/1678-4685-GMB-2024-0139
Ana Karoline Nunes-Alves, Jônatas Santos Abrahão, Sávio Torres de Farias
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引用次数: 0

摘要

本文采用结构分子模型分析了巴西Yaravirus brasiliense,这是一种感染阿米巴原虫的80纳米病毒,dsDNA长度为45 kbp。几乎所有的74个基因之前都被鉴定为orfan。考虑到其前所未有的遗传含量,我们分析了Yaravirus基因组,以了解其遗传组织,蛋白质组,以及它如何与宿主相互作用。我们报道了所有Yaravirus蛋白的可能功能。我们的研究结果首次报道了蛋白质组片段,其中蛋白质在模块中分离并在蛋白质水平上连接在一起。鉴于一些Yaravirus蛋白与三羧酸循环(TCA)、乙醛酸循环和呼吸复合物相关蛋白在结构上的相似性,我们的工作也允许我们假设这些病毒蛋白可能通过上调来调节细胞代谢。这些TCA循环相关酶的存在可能试图克服循环的控制点,因为它们是维持苹果酸和草酰乙酸水平的战略性蛋白质。因此,我们提出,Yaravirus蛋白将能量和资源重定向到病毒生产,并避开TCA周期控制点,“解锁”周期。总的来说,我们的数据帮助我们了解了一种以前几乎完全未知的病毒,并对病毒的惊人多样性有了更多的了解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Yaravirus brasiliense genomic structure analysis and its possible influence on the metabolism.

Here we analyze the Yaravirus brasiliense, an amoeba-infecting 80-nm-sized virus with a 45-kbp dsDNA, using structural molecular modeling. Almost all of its 74 genes were previously identified as ORFans. Considering its unprecedented genetic content, we analyzed Yaravirus genome to understand its genetic organization, its proteome, and how it interacts with its host. We reported possible functions for all Yaravirus proteins. Our results suggest the first ever report of a fragment proteome, in which the proteins are separated in modules and joined together at a protein level. Given the structural resemblance between some Yaravirus proteins and proteins related to tricarboxylic acid cycle (TCA), glyoxylate cycle, and the respiratory complexes, our work also allows us to hypothesize that these viral proteins could be modulating cell metabolism by upregulation. The presence of these TCA cycle-related enzymes specifically could be trying to overcome the cycle's control points, since they are strategic proteins that maintain malate and oxaloacetate levels. Therefore, we propose that Yaravirus proteins are redirecting energy and resources towards viral production, and avoiding TCA cycle control points, "unlocking" the cycle. Altogether, our data helped understand a previously almost completely unknown virus, and a little bit more of the incredible diversity of viruses.

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来源期刊
Genetics and Molecular Biology
Genetics and Molecular Biology 生物-生化与分子生物学
CiteScore
4.20
自引率
4.80%
发文量
111
审稿时长
3 months
期刊介绍: Genetics and Molecular Biology (formerly named Revista Brasileira de Genética/Brazilian Journal of Genetics - ISSN 0100-8455) is published by the Sociedade Brasileira de Genética (Brazilian Society of Genetics). The Journal considers contributions that present the results of original research in genetics, evolution and related scientific disciplines. Manuscripts presenting methods and applications only, without an analysis of genetic data, will not be considered.
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