FEMS microbiology ecology最新文献_第4页

Towards understanding the impact of mycorrhizal fungal environments on the functioning of terrestrial ecosystems. 了解菌根真菌环境对陆地生态系统功能的影响。

IF 3.2 3区生物学

FEMS microbiology ecology Pub Date : 2025-07-14 DOI: 10.1093/femsec/fiaf062

Olivier Nouwen, Francois Rineau, Petr Kohout, Petr Baldrian, Nico Eisenhauer, Natalie Beenaerts, Sofie Thijs, Jaco Vangronsveld, Nadejda A Soudzilovskaia

{"title":"Towards understanding the impact of mycorrhizal fungal environments on the functioning of terrestrial ecosystems.","authors":"Olivier Nouwen, Francois Rineau, Petr Kohout, Petr Baldrian, Nico Eisenhauer, Natalie Beenaerts, Sofie Thijs, Jaco Vangronsveld, Nadejda A Soudzilovskaia","doi":"10.1093/femsec/fiaf062","DOIUrl":"10.1093/femsec/fiaf062","url":null,"abstract":"Mutualistic interactions between plants and soil fungi, mycorrhizas, control carbon and nutrient fluxes in terrestrial ecosystems. Soil of ecosystems featuring a particular type of mycorrhiza exhibit specific properties across multiple dimensions of soil functioning. The knowledge about the impacts of mycorrhizal fungi on soil functioning accumulated so far, indicates that these impacts are of major importance, yet poorly conceptualized. We propose a concept of mycorrhizal fungal environments in soil. Within this concept, we discuss knowledge gaps related to the understanding and quantification of mycorrhizal fungal impacts. We introduce an experimental framework to address these gaps in a quantitative manner, and present the field experiment 'Mycotron', where we established vegetation series featuring three mycorrhizal types; ericoid (ERM), ecto- (ECM), and arbuscular mycorrhiza (AM), to quantitatively assess mycorrhizal fungal impacts on soil functioning. The experimental treatments entail manipulations in dominance levels of vegetation of three mycorrhizal types (AM, ECM, and ERM) in standardized soil conditions. This experiment constitutes a unique testbed to quantitatively evaluate the impacts of distinct mycorrhizal fungal environments on a large variety of ecosystem functions. Our approach aids the quantification of microbiota and plant-microbial interaction impacts on soil biochemical cycles.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12320777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144283167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impacts of diverse undersown cover crops on seasonal soil microbial properties. 不同地下覆盖作物对季节性土壤微生物特性的影响。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2025-06-24 DOI: 10.1093/femsec/fiaf068

Rashmi Shrestha, Karoliina Huusko, Outi-Maaria Sietiö, Bernhard Schmid, Seraina Lisa Cappeli, Paula Thitz, Stephanie Gerin, Anna-Liisa Laine, Annalea Lohila, Jussi Heinonsalo

{"title":"Impacts of diverse undersown cover crops on seasonal soil microbial properties.","authors":"Rashmi Shrestha, Karoliina Huusko, Outi-Maaria Sietiö, Bernhard Schmid, Seraina Lisa Cappeli, Paula Thitz, Stephanie Gerin, Anna-Liisa Laine, Annalea Lohila, Jussi Heinonsalo","doi":"10.1093/femsec/fiaf068","DOIUrl":"10.1093/femsec/fiaf068","url":null,"abstract":"Positive relationships between plant diversity, microbial diversity, and ecosystem functioning have widely been observed in experimental grasslands. However, the impact of cover crop (CC) species diversification on soil microbial diversity and function in croplands remains underexplored. This study investigated how increasing the diversity of undersown CCs affected seasonal properties of soil microbiomes and whether these changes resulted in legacy effects on next-year crops. In barley fields undersown with functionally diverse CCs, soil samples were collected throughout the year to assess microbial properties. To evaluate legacy effects on the following year's barley, soil microorganisms were sequenced from spring samples collected before CC termination. Additionally, a pot experiment using flax was conducted to study how CC diversity influenced arbuscular mycorrhizal (AM) fungal colonization in roots. Results showed that vegetation presence and higher CC richness increased microbial biomass carbon and decreased the microbial metabolic quotient. Legumes' presence reduced microbial respiration. Fungal and AM fungal diversity also increased with CC richness, while legumes helped suppress fungal pathogens. In the pot experiment, presence of both vegetation and legumes positively influenced AM fungal root colonization. Overall, undersowing diverse CCs, particularly legumes, can increase soil microbial diversity and soil health, benefiting both the current and next-year crops.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12231134/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial custody: key microbiome inhabitant Sphingomonas alleviates silver nanoparticle toxicity in Daphnia magna. 微生物保管：关键微生物群居民鞘氨单胞菌减轻银纳米颗粒对大水蚤的毒性。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2025-06-24 DOI: 10.1093/femsec/fiaf061

Jesse Ouwehand, Bregje W Brinkmann, Willie J G M Peijnenburg, Martina G Vijver

{"title":"Microbial custody: key microbiome inhabitant Sphingomonas alleviates silver nanoparticle toxicity in Daphnia magna.","authors":"Jesse Ouwehand, Bregje W Brinkmann, Willie J G M Peijnenburg, Martina G Vijver","doi":"10.1093/femsec/fiaf061","DOIUrl":"10.1093/femsec/fiaf061","url":null,"abstract":"Increased usage of nanotechnological applications inevitably leads to exposure of hosts and their associated microbiomes to metallic nanoparticles. Various bacteria within the microbiome harbour mechanisms to protect themselves against metal-related toxicity. These mechanisms have been broadly described in the absence of a host. Here, we studied how silver ion-resistant bacteria isolated from the Daphnia magna microbiome shape the host's exposure to silver nanoparticles. With germfree and mono-associated neonates, the effects of these microbes on the sensitivity of D. magna to silver nanoparticles were studied. By using this approach, a core member of the D. magna microbiome Sphingomonas yanoikuyae was identified to be silver-resistant. Neonates mono-associated with S. yanoikuyae were as sensitive to silver nanoparticles as naturally colonized neonates, whereas mono-association with Microbacterium and germfree neonates had increased sensitivity. Silver ions are the major attribution to toxicity in germfree and Microbacterium-associated neonates, whereas particles contribute more to the toxicity for the naturally- and Sphingomonas-colonized neonates. Sphingomonas accumulated in vivo more silver ions from its local environment than the other D. magna bacterial isolates. The current study shows that bacteria can play a vital role in shaping the speciation of nanomaterials and thereby modifying the toxicity to hosts.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12199703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial dynamics across tri-trophic systems: insights from plant-herbivore-predator interactions. 微生物动力学跨越三营养系统：从植物-草食-捕食相互作用的见解。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2025-06-24 DOI: 10.1093/femsec/fiaf065

Hong Yan, Endong Wang, Guo-Shu Wei, Xuenong Xu, Mark R H Hurst, Bo Zhang

{"title":"Microbial dynamics across tri-trophic systems: insights from plant-herbivore-predator interactions.","authors":"Hong Yan, Endong Wang, Guo-Shu Wei, Xuenong Xu, Mark R H Hurst, Bo Zhang","doi":"10.1093/femsec/fiaf065","DOIUrl":"10.1093/femsec/fiaf065","url":null,"abstract":"Microbes play a critical role in regulating tri-trophic interactions among plants, herbivores, and their natural enemies, influencing key ecological and evolutionary processes. To fully understand these interactions through the food chain, a well-defined tri-trophic system is required. We investigated microbial dynamics involving plants (beans, cucumbers, and eggplants), spider mites (Tetranychus urticae), and predatory mites (Phytoseiulus persimilis) through 16S rRNA gene sequencing. The results revealed significant variations in microbiota across different trophic levels. Source tracking analysis indicated that microbiota at each trophic level were rarely inherited from the previous one, and deterministic processes played a key role in shaping the endosphere communities of these levels. Most shared zero-radius operational taxonomic units across each trophic level belonged to Pseudomonas, Bacillus, and Staphylococcus. Leaf microbiota differed among plants, while spider mites harbored similar microbiota. Notably, the microbiota of predatory mites on eggplants differed significantly from those on the other two plants. Biomarker selection and correlation analyses revealed that the abundance of Methylobacterium and Stenotrophomonas was strongly correlated with the improved fitness of predatory mites across different plants. Our study highlights the complex and dynamic nature of microbial communities across different trophic levels in a well-defined plant-herbivore-predator system.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":"101 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12199696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144495440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing the efficiency and the side effects of atrazine-degrading biocomposites amended to atrazine-contaminated soil. 评价阿特拉津污染土壤中降解阿特拉津生物复合材料的效率和副作用。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2025-06-24 DOI: 10.1093/femsec/fiaf071

Sakineh Abbasi, Marion Devers-Lamrani, Fabrice Martin-Laurent, Caroline Michel, Sana Romdhane, Nadine Rouard, Aymé Spor

{"title":"Assessing the efficiency and the side effects of atrazine-degrading biocomposites amended to atrazine-contaminated soil.","authors":"Sakineh Abbasi, Marion Devers-Lamrani, Fabrice Martin-Laurent, Caroline Michel, Sana Romdhane, Nadine Rouard, Aymé Spor","doi":"10.1093/femsec/fiaf071","DOIUrl":"10.1093/femsec/fiaf071","url":null,"abstract":"Even decades after being banned in Europe, atrazine and its main metabolites can still be found in soils. While bioaugmentation using pesticide-degrading bacteria is already employed for remediating polluted soils, there is a need to improve its efficiency. Investigating the use of carrier materials to deliver pesticide-degrading microorganisms in situ emerges as a promising approach. Here, we generated atrazine-degrading biocomposites by cultivating either a bacterial strain or a four-species consortium on zeolite as the carrier material. Using a microcosm approach, we evaluated their efficiency to mineralize 14C-atrazine in soil compared to free-living cells, and assessed their side effects on the native soil bacterial community using 16S rRNA metabarcoding. We showed that, right after inoculation, atrazine mineralization potential of the free-living cells was higher than that of the biocomposites. However, microcosms inoculated with the biocomposites displayed significantly higher atrazine mineralization potential after 15 and 45 days of incubation, indicating a higher efficiency but also a better stability in soil. Inoculation of free-living cells and biocomposites differently influenced the diversity and composition of the native microbial community, their impacts being modulated by the atrazine contamination scenario. Altogether, our results provide a thorough evaluation of the efficiency and the ecological impact of atrazine-degrading biocomposites in soil.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12231133/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Oral amoxicillin treatment disrupts the gut microbiome and metabolome without interfering with luminal redox potential in the intestine of Wistar Han rats. 更正：口服阿莫西林治疗会破坏肠道微生物组和代谢组，而不会干扰Wistar Han大鼠肠道内的腔内氧化还原电位。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2025-06-24 DOI: 10.1093/femsec/fiaf070

引用次数: 0

Microbial community dynamics in two Central European peatlands affected by different nitrogen depositions. 不同氮沉降对中欧两个泥炭地微生物群落动态的影响

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2025-06-24 DOI: 10.1093/femsec/fiaf056

Jiri Barta, Hana Santruckova, Martin Novak, Bohuslava Cejkova, Ivana Jackova, Frantisek Buzek, Marketa Stepanova, Jan Curik, Frantisek Veselovsky, Eva Prechova

{"title":"Microbial community dynamics in two Central European peatlands affected by different nitrogen depositions.","authors":"Jiri Barta, Hana Santruckova, Martin Novak, Bohuslava Cejkova, Ivana Jackova, Frantisek Buzek, Marketa Stepanova, Jan Curik, Frantisek Veselovsky, Eva Prechova","doi":"10.1093/femsec/fiaf056","DOIUrl":"10.1093/femsec/fiaf056","url":null,"abstract":"Changes in organic matter accumulation in wetlands are critical for climate dynamics. Different nitrogen (N) inputs in Sphagnum-dominated peat bogs can lead to varying rates of carbon (C) and N accumulation, influencing greenhouse gas emissions. We investigated how contrasting N deposition shapes microbial communities in two Czech peat bogs, focusing on biological N2 fixation (BNF) as a key N input in pristine wetlands. Higher N deposition resulted in a more active microbial community with increased enzyme activity and C acquisition, potentially accelerating decomposition and reducing C storage. Enhanced denitrification, indicated by active nosZ Clade I genes, suggests that higher N inputs may increase N losses through denitrification. In contrast, the lower N site showed a less active microbial community with slower decomposition, beneficial for C sequestration, though potentially less adaptable to future N increases. Experimental BNF rates were 70 times higher at the high N site, consistent with elevated diazotroph activity indicated by active nifH gene. Phosphorus (P) availability and NH4+/NO3- ratios appeared to drive BNF differences, emphasizing the need for managed N inputs to maintain peatland ecological functions.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial communities in glacial lakes of Glacier National Park, MT, USA. 美国MT冰川国家公园冰川湖中的微生物群落。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2025-06-24 DOI: 10.1093/femsec/fiaf060

Logan M Peoples, J Joseph Giersch, Tyler H Tappenbeck, Joseph W Vanderwall, John M Ranieri, Trista J Vick-Majors, James J Elser, Matthew J Church

{"title":"Microbial communities in glacial lakes of Glacier National Park, MT, USA.","authors":"Logan M Peoples, J Joseph Giersch, Tyler H Tappenbeck, Joseph W Vanderwall, John M Ranieri, Trista J Vick-Majors, James J Elser, Matthew J Church","doi":"10.1093/femsec/fiaf060","DOIUrl":"10.1093/femsec/fiaf060","url":null,"abstract":"Glaciers are retreating, altering alpine ecosystems and creating new proglacial lakes. Compared to lakes fed by snowpack, glacial lakes are often enriched in nutrients and suspended solids that decrease light penetration. However, the microorganisms and biogeochemical conditions within these newly formed lakes are not well characterized. We describe the microbial communities in 14 glacial lakes in Glacier National Park, MT, USA using 16S rRNA gene amplicon sequencing and measurements of nutrient concentrations, water clarity, and other environmental properties. Microbial communities were distinct between lakes, including those connected to the same glacier, indicating the importance of site-specific biogeochemical and physical dynamics on these systems. Microbial community composition correlated with lake age (formation before or after the Little Ice Age) and conductivity but not with whether a lake was connected to a contemporaneous glacier > 0.1 km2. Heterotrophic lineages found in other glacial systems were abundant and widespread, while cyanobacteria only reached appreciable abundances in shallow lakes where light reached the benthos. Relative abundances of ammonia and nitrite oxidizers correlated with concentrations of nitrate and nitrite, suggesting nitrification may help control nitrogen forms and concentrations in glacial lakes. We show that as glaciers recede, unique glacial lake microbial communities will be formed and lost with them.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12199700/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and potential of the hyperparasite Acremonium persicinum as biocontrol agent against coffee leaf rust. 咖啡叶锈病高寄生物桃蚜的鉴定及防治潜力。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2025-06-24 DOI: 10.1093/femsec/fiaf064

Minli Liang, Yanqiong Liang, Xing Huang, Xuehui Bai, Ying Lu, Bo Wang, Shibei Tan, Helong Chen, Chunping He, Zhenjia Chen, Kexian Yi, Weihuai Wu

{"title":"Identification and potential of the hyperparasite Acremonium persicinum as biocontrol agent against coffee leaf rust.","authors":"Minli Liang, Yanqiong Liang, Xing Huang, Xuehui Bai, Ying Lu, Bo Wang, Shibei Tan, Helong Chen, Chunping He, Zhenjia Chen, Kexian Yi, Weihuai Wu","doi":"10.1093/femsec/fiaf064","DOIUrl":"10.1093/femsec/fiaf064","url":null,"abstract":"Coffee leaf rust (CLR) caused by Hemileia vastatrix, has emerged as a growing threat to coffee production in China. This study focused on the identification and characterization of the hyperparasitic fungus Acremonium persicinum using integrated plant pathology and molecular biology approaches. The spore suspension of the fungal strain HY85 exhibited a 91.18% inhibition rate against the germination of H. vastatrix urediniospores. Inoculation of coffee leaf discs with H. vastatrix urediniospores resulted in the development of visible chlorotic lesions after 16 days. However, other inoculation methods did not yield early chlorotic lesions, indicating an absence of H. vastatrix infection. Quantitative PCR analysis revealed that the copy numbers of H. vastatrix DNA after 16 days postinoculation were 1.41 × 108, 7.59 × 108, and 1.66 × 108, respectively. Notably, H. vastatrix DNA was undetectable in leaf discs coinoculated with H. vastatrix urediniospores and the hyperparasitic strain HY85, suggesting complete suppression of the pathogen. In vitro lesion control experiments demonstrated that 96 h after inoculation with HY85, the characteristic yellow urediniospore masses on the lesions were entirely replaced by the white mycelium of the hyperparasitic fungus. The control efficacy of strain HY85 against coffee rust fungus was 66.67%. Scanning electron microscopy revealed that strain HY85 caused significant morphological alterations in the urediniospores, including indentation and collapse, leading to severe structural damage. These findings underscore the capability of A. persicinum to disrupt the life cycle of H. vastatrix and its potential as an effective biocontrol agent strain for CLR management.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12207870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The quest for molecular markers indicating root growth in microbially treated tomato (Solanum lycopersicum) plants. 微生物处理番茄根系生长分子标记的探索。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2025-06-24 DOI: 10.1093/femsec/fiaf063

Leonard S van Overbeek, Stefan Aanstoot, Erik Esveld, Lina Russ, Beatriz Andreo Jimenez

{"title":"The quest for molecular markers indicating root growth in microbially treated tomato (Solanum lycopersicum) plants.","authors":"Leonard S van Overbeek, Stefan Aanstoot, Erik Esveld, Lina Russ, Beatriz Andreo Jimenez","doi":"10.1093/femsec/fiaf063","DOIUrl":"10.1093/femsec/fiaf063","url":null,"abstract":"Roots are essential plant organs for anchorage in soil, uptake of water with nutrients, storage of photosynthates, and microbial interactions. More knowledge on microorganisms stimulating root growth is needed to control root development of cultured plants. A marker-assisted approach would facilitate vast screenings of microbes for eventual effects on root development. It was aimed to select for transcripts that report on root growth stimulation at the early tomato plant growth stage upon microbial treatments. Microbially treated tomato (Solanum lycopersicum) plants were cultivated in stone wool slabs and screened for genes that increased or decreased in differential expression upon increased root growth, by RNAseq. Expression of 21 selected genes was measured by quantitative polymerase chain reaction (qPCR) in relation with stimulated root growth, recorded by X-ray microtomography, of microbially treated tomato plants cultivated in stone wool blocks. Two genes were identified of which expression significantly correlated with high measured root length, and for one also with high measured shoot wet and dry weight. The translated products, both involved in modulation of Rubisco activity, were a chloroplast-localized acetyltransferase (SlSNAT2) and a Rubisco activase (Rca). Transcripts whose translated products modulate Rubisco activity can serve as candidates for reporting on early root development upon microbial inoculation.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":"101 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12199702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144495441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0