FEMS microbiology ecology最新文献

An interdisciplinary perspective of the built-environment microbiome.

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-12-19 DOI: 10.1093/femsec/fiae166

John S McAlister, Michael J Blum, Yana Bromberg, Nina H Fefferman, Qiang He, Eric Lofgren, Debra L Miller, Courtney Schreiner, K Selcuk Candan, Heather Szabo-Rogers, J Michael Reed

{"title":"An interdisciplinary perspective of the built-environment microbiome.","authors":"John S McAlister, Michael J Blum, Yana Bromberg, Nina H Fefferman, Qiang He, Eric Lofgren, Debra L Miller, Courtney Schreiner, K Selcuk Candan, Heather Szabo-Rogers, J Michael Reed","doi":"10.1093/femsec/fiae166","DOIUrl":"https://doi.org/10.1093/femsec/fiae166","url":null,"abstract":"The built environment provides an excellent setting for interdisciplinary research on the dynamics of microbial communities. The system is simplified compared to many natural settings, and to some extent the entire environment can be manipulated, from architectural design to materials use, air flow, human traffic, and capacity to disrupt microbial communities through cleaning. Here we provide an overview of the ecology of the microbiome in the built environment. We address niche space and refugia, population and community (metagenomic) dynamics, spatial ecology within a building, including the major microbial transmission mechanisms, as well as evolution. We also address landscape ecology, connecting microbiomes between physically separated buildings. At each stage we pay particular attention to the actual and potential interface between disciplines, such as ecology, epidemiology, materials science, and human social behavior. We end by identifying some opportunities for future interdisciplinary research on the microbiome of the built environment.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142863690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The type of environment has a greater impact on the larval microbiota of Anopheles arabiensis than on the microbiota of their breeding water.

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-12-18 DOI: 10.1093/femsec/fiae161

Lorenzo Assentato, Louise K J Nilsson, Carl Brunius, Vilhelm Feltelius, Rasmus Elleby, Richard J Hopkins, Olle Terenius

{"title":"The type of environment has a greater impact on the larval microbiota of Anopheles arabiensis than on the microbiota of their breeding water.","authors":"Lorenzo Assentato, Louise K J Nilsson, Carl Brunius, Vilhelm Feltelius, Rasmus Elleby, Richard J Hopkins, Olle Terenius","doi":"10.1093/femsec/fiae161","DOIUrl":"https://doi.org/10.1093/femsec/fiae161","url":null,"abstract":"Mosquito larvae of the genus Anopheles develop entirely in water, frequently visiting the surface for air. The aquatic environment plays a key role in shaping their microbiota, but the connection between environmental characteristics of breeding sites and larval microbiota remains underexplored. This study focuses on Anopheles arabiensis, which inhabits the surface microlayer (SML) of breeding sites, a zone with high particle density. We hypothesized that the SML could allow us to capture the diversity of the surrounding environment, and in turn its influence on the larval microbial communities. To test this, we collected Anopheles arabiensis larvae and SML samples from various breeding sites categorized by environmental features. Our results confirm that breeding site characteristics are significant drivers of the bacterial species present in mosquito larvae. Additionally, we found that the larval micro-environment selectively shapes its microbiota, highlighting a dynamic interplay between environmental and internal factors. Interestingly, specific bacterial families were associated with the presence or absence of larvae in breeding sites, suggesting potential ecological roles. These findings expand our understanding of vector-mosquito microbiota, emphasizing the importance of breeding site features in shaping larval microbial communities and providing a foundation for future research on mosquito ecology and control strategies.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142853381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extensive environmental survey of free-living amoebae and their elusive association with Mycobacterium bovis or Mycobacterium avium subsp. paratuberculosis.

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-12-17 DOI: 10.1093/femsec/fiae164

Amélie Jessu, Thierry Cochard, Mélanie Burtin, Stéphanie Crapart, Vincent Delafont, Ascel Samba-Louaka, Franck Biet, Jean-Louis Moyen, Yann Héchard

{"title":"Extensive environmental survey of free-living amoebae and their elusive association with Mycobacterium bovis or Mycobacterium avium subsp. paratuberculosis.","authors":"Amélie Jessu, Thierry Cochard, Mélanie Burtin, Stéphanie Crapart, Vincent Delafont, Ascel Samba-Louaka, Franck Biet, Jean-Louis Moyen, Yann Héchard","doi":"10.1093/femsec/fiae164","DOIUrl":"https://doi.org/10.1093/femsec/fiae164","url":null,"abstract":"Free-living amoebae (FLA) are described as environmental reservoirs for some bacteria able to resist their phagocytosis. In the environment, the fate of Mycobacterium bovis (Mbo) and Mycobacterium avium subsp. paratuberculosis (Map), responsible for bovine tuberculosis and paratuberculosis respectively, remains poorly understood and is considered potentially problematic in the eradication and control of these diseases. We hypothesize that FLA may play a role in the persistence of Mbo and Map in the environment. In this study, 90 samples were collected from herds affected by one or both diseases to investigate the diversity of amoeba and their associated bacteria. Metabarcoding analyses revealed that Acanthamoeba, Copromyxa, Naegleria and Vermamoeba were the most represented genera of FLA, with Pseudomonadota being the bacteria most commonly found associated with FLA. Although no Mbo and Map DNA were identified by sequencing, traces were detected by ddPCR, specifically targeting these bacteria. In conclusion, we described a wide diversity of FLA and associated bacteria in this environment. It also suggests that Map and Mbo could be associated, even weakly, with FLA in the environment. However, this needs to be confirmed by detecting a highest amount of DNA and if possible, cultivable Map and/or Mbo associated with these environmental FLA.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of anthropogenic influences on a tropical lake ecosystem and its surrounding catchment: a case study of Lake Sentani.

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-12-17 DOI: 10.1093/femsec/fiae162

Sulung Nomosatryo, Daniel Lipus, Alexander Bartholomäus, Cynthia Henny, Iwan Ridwansyah, Puguh Sujarta, Sizhong Yang, Dirk Wagner, Jens Kallmeyer

{"title":"The role of anthropogenic influences on a tropical lake ecosystem and its surrounding catchment: a case study of Lake Sentani.","authors":"Sulung Nomosatryo, Daniel Lipus, Alexander Bartholomäus, Cynthia Henny, Iwan Ridwansyah, Puguh Sujarta, Sizhong Yang, Dirk Wagner, Jens Kallmeyer","doi":"10.1093/femsec/fiae162","DOIUrl":"https://doi.org/10.1093/femsec/fiae162","url":null,"abstract":"Lake Sentani is a tropical lake in Indonesia, consisting of four interconnected sub-basins of different water depths. While previous work has highlighted the impact of catchment composition on biogeochemical processes in Lake Sentani, little is currently known about the microbiological characteristics across this unique ecosystem. With recent population growth in this historically rural area, the anthropogenic impact on Lake Sentani and hence its microbial life is also increasing. Therefore, we aimed to explore the influence of environmental and anthropogenic factors on the microbial diversity of Lake Sentani. Here we present a detailed microbiological evaluation of Lake Sentani, analyzing 49 different sites across the lake, its tributary rivers and their river mouths to assess diversity and community structure using 16S rRNA gene sequencing. Our results reveal distinct communities in lake and river sediments, supporting the observed geochemical differences. Taxonomic assessment showed the potential impact of anthropogenic pressure along the northern, urbanized shore, as river and river mouth samples revealed high abundances of Bacteroidota, Firmicutes and Cyanobacteria, which could be attributed to pollution and eutriphication. In contrast, lake sediment communities were dominated by Thermodesulfovibrionia, Methanomethylicia, Bathyarchaeia and Thermoplasmata, suggesting sulfate reducing, thermophilic, acidophilic bacteria and methanogenic archaea to play an important role in tropical lake systems. This study provides novel insights into ecological functions of tropical lakes and contributes to the optimization of management strategies of Lake Sentani, ensuring its holistic preservation in the future.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental evidence on the impact of climate-induced hydrological and thermal variations on glacier-fed stream biofilms. 气候引起的水文和热量变化对冰川溪流生物膜影响的实验证据。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-12-14 DOI: 10.1093/femsec/fiae163

David Touchette, Martina Gonzalez Mateu, Grégoire Michoud, Nicola Deluigi, Ramona Marasco, Daniele Daffonchio, Hannes Peter, Tom Battin

{"title":"Experimental evidence on the impact of climate-induced hydrological and thermal variations on glacier-fed stream biofilms.","authors":"David Touchette, Martina Gonzalez Mateu, Grégoire Michoud, Nicola Deluigi, Ramona Marasco, Daniele Daffonchio, Hannes Peter, Tom Battin","doi":"10.1093/femsec/fiae163","DOIUrl":"https://doi.org/10.1093/femsec/fiae163","url":null,"abstract":"Climate change is predicted to alter the hydrological and thermal regimes of high-mountain streams, particularly glacier-fed streams. However, relatively little is known about how these environmental changes impact the microbial communities in glacier-fed streams. Here, we operated streamside flume mesocosms in the Swiss Alps, where benthic biofilms were grown under treatments simulating climate change. Treatments comprised four flow (natural, intermittent, stochastic, and constant) and two temperature (ambient streamwater and warming of +2 °C) regimes. We monitored microbial biomass, diversity, community composition, and metabolic diversity in biofilms over three months. We found that community composition was largely influenced by successional dynamics independent of the treatments. While stochastic and constant flow regimes did not significantly affect community composition, droughts altered their composition in the intermittent regime, favouring drought-adapted bacteria and decreasing algal biomass. Concomitantly, warming decreased algal biomass and the abundance of some typical glacier-fed stream bacteria and eukaryotes, and stimulated heterotrophic metabolism overall. Our study provides experimental evidence toward potential and hitherto poorly considered impacts of climate change on benthic biofilms in glacier-fed streams.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and networking of uni-cyanobacterial cultures and associated heterotrophic bacteria from the benthic microbial mat of a desert hydrothermal spring. 沙漠热液泉底栖微生物垫中单青霉菌培养物和相关异养菌的多样性和网络化。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-11-23 DOI: 10.1093/femsec/fiae148

Khaoula Lassoued, Mouna Mahjoubi, Elias Asimakis, Naima Bel Mokhtar, Panagiota Stathopoulou, Refka Ben Hamouda, Olfa Bousselmi, Ramona Marasco, Ahmed Slaheddine Masmoudi, Daniele Daffonchio, George Tsiamis, Ameur Cherif

{"title":"Diversity and networking of uni-cyanobacterial cultures and associated heterotrophic bacteria from the benthic microbial mat of a desert hydrothermal spring.","authors":"Khaoula Lassoued, Mouna Mahjoubi, Elias Asimakis, Naima Bel Mokhtar, Panagiota Stathopoulou, Refka Ben Hamouda, Olfa Bousselmi, Ramona Marasco, Ahmed Slaheddine Masmoudi, Daniele Daffonchio, George Tsiamis, Ameur Cherif","doi":"10.1093/femsec/fiae148","DOIUrl":"10.1093/femsec/fiae148","url":null,"abstract":"Thermal springs harbour microorganisms, often dominated by cyanobacteria, which form biofilms and microbial mats. These phototrophic organisms release organic exudates into their immediate surroundings, attracting heterotrophic bacteria that contribute to the diversity and functioning of these ecosystems. In this study, the microbial mats from a hydrothermal pool in the Ksar Ghilane oasis in the Grand Erg Oriental of the Desert Tunisia were collected to obtain cyanobacterial cultures formed by single cyanobacterial species. High-throughput analysis showed that while the microbial mat hosted diverse cyanobacteria, laboratory cultures selectively enriched cyanobacteria from the Leptolyngbya, Nodosilinea, and Arthronema. Per each of these genera, multiple non-axenic uni-cyanobacterial cultures were established, totalling 41 cultures. Cyanobacteria taxa mediated the assembly of distinct heterotrophic bacterial communities, with members of the Proteobacteria and Actinobacteria phyla dominating. The bacterial communities of uni-cyanobacterial cultures were densely interconnected, with heterotrophic bacteria preferentially co-occurring with each other. Our study highlighted the complex structures of non-axenic uni-cyanobacterial cultures, where taxonomically distinct cyanobacteria consistently associate with specific groups of heterotrophic bacteria. The observed associations were likely driven by common selection pressures in the laboratory, such as cultivation conditions and specific hosts, and may not necessarily reflect the microbial dynamic occurring in the spring microbial mats.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling detoxifying symbiosis and dietary influence on the Southern green shield bug microbiota. 揭示解毒共生和饮食对南方绿盾蝽微生物群的影响

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-11-23 DOI: 10.1093/femsec/fiae150

Magda A Rogowska-van der Molen, Hristina V Savova, Elke A T Janssen, Theo van Alen, Silvia Coolen, Robert S Jansen, Cornelia U Welte

{"title":"Unveiling detoxifying symbiosis and dietary influence on the Southern green shield bug microbiota.","authors":"Magda A Rogowska-van der Molen, Hristina V Savova, Elke A T Janssen, Theo van Alen, Silvia Coolen, Robert S Jansen, Cornelia U Welte","doi":"10.1093/femsec/fiae150","DOIUrl":"10.1093/femsec/fiae150","url":null,"abstract":"The Southern green shield bug, Nezara viridula, is an invasive piercing and sucking pest insect that feeds on crops and poses a threat to global food production. Insects live in close relationships with microorganisms providing their host with unique capabilities, such as resistance to toxic plant metabolites. In this study, we investigated the resistance to and detoxification of the plant metabolite 3-nitropropionic acid (NPA) by core and transient members of the N. viridula microbial community. Microbial community members showed a different tolerance to the toxin and we determined that six out of eight strains detoxified NPA. Additionally, we determined that NPA might interfere with the biosynthesis and transport of l-leucine. Moreover, our study explored the influence of diet on the gut microbial composition of N. viridula, demonstrating that switching to a single-plant diet shifts the abundance of core microbes. In line with this, testing pairwise microbial interactions revealed that core microbiota members support each other and repress the growth of transient microorganisms. With this work, we provide novel insights into the factors shaping the insect gut microbial communities and demonstrate that N. viridula harbours many toxin-degrading bacteria that could support its resistance to plant defences.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental factors and potential probiotic lineages shape the active prokaryotic communities associated with healthy Penaeus stylirostris larvae and their rearing water. 环境因素和潜在的益生菌系形成了与健康的青花鱼幼体及其饲养水相关的活性原核生物群落。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-11-23 DOI: 10.1093/femsec/fiae156

Carolane Giraud, Nelly Wabete, Célia Lemeu, Nazha Selmaoui-Folcher, Dominique Pham, Viviane Boulo, Nolwenn Callac

{"title":"Environmental factors and potential probiotic lineages shape the active prokaryotic communities associated with healthy Penaeus stylirostris larvae and their rearing water.","authors":"Carolane Giraud, Nelly Wabete, Célia Lemeu, Nazha Selmaoui-Folcher, Dominique Pham, Viviane Boulo, Nolwenn Callac","doi":"10.1093/femsec/fiae156","DOIUrl":"10.1093/femsec/fiae156","url":null,"abstract":"Microbial dysbiosis is hypothesized to cause larval mass mortalities in New Caledonian shrimp hatcheries. In order to confirm this hypothesis and allow further microbial comparisons, we studied the active prokaryotic communities of healthy Penaeus stylirostris larvae and their surrounding environment during the first 10 days of larval rearing. Using daily nutrient concentration quantitative analyses and spectrophotometric organic matter analyses, we highlighted a global eutrophication of the rearing environment. We also evidenced drastic bacterial community modifications in the water and the larvae samples using Illumina HiSeq sequencing of the V4 region of the 16S rRNA gene. We confirmed that Alteromonadales, Rhodobacterales, Flavobacteriales, Oceanospirillales, and Vibrionales members formed the core bacteriota of shrimp larvae. We also identified, in the water and the larvae samples, several potential probiotic bacterial strains that could lead to rethink probiotic use in aquaculture (AEGEAN 169 marine group, OM27 clade, Ruegeria, Leisingera, Pseudoalteromonas, and Roseobacter). Finally, investigating the existing correlations between the environmental factors and the major bacterial taxa of the water and the larvae samples, we suggested that deterministic and stochastic processes were involved in the assembly of prokaryotic communities during the larval rearing of P. stylirostris. Overall, our results showed that drastic changes mostly occurred during the zoea stages suggesting that this larval phase is crucial during shrimp larval development.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metatranscriptomics provide insights into the role of the symbiont Midichloria mitochondrii in Ixodes ticks. 元转录组学揭示了线粒体共生体敌敌畏在蜱中的作用。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-11-23 DOI: 10.1093/femsec/fiae133

Laurene Leclerc, John Mattick, Brendan P Burns, Davide Sassera, Julie Dunning Hotopp, Nathan Lo

{"title":"Metatranscriptomics provide insights into the role of the symbiont Midichloria mitochondrii in Ixodes ticks.","authors":"Laurene Leclerc, John Mattick, Brendan P Burns, Davide Sassera, Julie Dunning Hotopp, Nathan Lo","doi":"10.1093/femsec/fiae133","DOIUrl":"10.1093/femsec/fiae133","url":null,"abstract":"Ticks are important vectors of bacterial, viral, and protozoan pathogens of humans and animals worldwide. Candidatus Midichloria mitochondrii is a highly abundant bacterial endosymbiont found in many tick species, including two medically important ticks respectively found in Europe and Australia, Ixodes ricinus and Ixodes holocyclus. The present study aimed to determine the symbiont's biological role by identifying lateral gene transfer (LGT) events, characterizing the transcriptome, and performing differential expression analyses. Metatranscriptomic data revealed that M. mitochondrii species in I. ricinus and I. holocyclus were equipped with the metabolic potential and were actively transcribing the genes for several important roles including heme, biotin and folate synthesis, oxidative stress response, osmotic regulation, and ATP production in microaerobic conditions. Differential expression analyses additionally showed an upregulation in stringent response and DNA repair genes in M. mitochondrii of I. holocyclus nymphs compared to adults. Low rates of differential expression suggest the symbiont may lack global gene regulation, as observed in other endosymbionts. Moreover, the identification of an LGT event and the proposed specialization of the M. mitochondrii strains, mIxholo1 and mIxholo2, for different I. holocyclus life stages highlight the complex interactions between M. mitochondrii and their tick hosts.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142375368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidics for studying the deep underground biosphere: from applications to fundamentals. 研究地下深层生物圈的微流体技术：从应用到基础。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-11-23 DOI: 10.1093/femsec/fiae151

Sandy Morais, Emeline Vidal, Anaïs Cario, Samuel Marre, Anthony Ranchou-Peyruse

{"title":"Microfluidics for studying the deep underground biosphere: from applications to fundamentals.","authors":"Sandy Morais, Emeline Vidal, Anaïs Cario, Samuel Marre, Anthony Ranchou-Peyruse","doi":"10.1093/femsec/fiae151","DOIUrl":"10.1093/femsec/fiae151","url":null,"abstract":"In this review, selected examples are presented to demonstrate how microfluidic approaches can be utilized for investigating microbial life from deep geological environments, both from practical and fundamental perspectives. Beginning with the definition of the deep underground biosphere and the conventional experimental techniques employed for these studies, the use of microfluidic systems for accessing critical parameters of deep life in geological environments at the microscale is subsequently addressed (high pressure, high temperature, low volume). Microfluidics can simulate a range of environmental conditions on a chip, enabling rapid and comprehensive studies of microbial behavior and interactions in subsurface ecosystems, such as simulations of porous systems, interactions among microbes/microbes/minerals, and gradient cultivation. Transparent microreactors allow real-time, noninvasive analysis of microbial activities (microscopy, Raman spectroscopy, FTIR microspectroscopy, etc.), providing detailed insights into biogeochemical processes and facilitating pore-scale analysis. Finally, the current challenges and opportunities to expand the use of microfluidic methodologies for studying and monitoring the deep biosphere in real time under deep underground conditions are discussed.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0