FEMS microbiology ecology最新文献_第2页

Functional stability despite structural changes in freshwater biofilm communities exposed to an antibiotic and an herbicide - the role of nutrient conditions. 暴露于抗生素和除草剂的淡水生物膜群落结构变化后的功能稳定性——营养条件的作用。

IF 3.2 3区生物学

FEMS microbiology ecology Pub Date : 2025-09-22 DOI: 10.1093/femsec/fiaf094

Sophie Oster, Eric Bollinger, Verena C Schreiner, Tobias Schmitt, Sabine Filker, Mirco Bundschuh

{"title":"Functional stability despite structural changes in freshwater biofilm communities exposed to an antibiotic and an herbicide - the role of nutrient conditions.","authors":"Sophie Oster, Eric Bollinger, Verena C Schreiner, Tobias Schmitt, Sabine Filker, Mirco Bundschuh","doi":"10.1093/femsec/fiaf094","DOIUrl":"10.1093/femsec/fiaf094","url":null,"abstract":"Freshwater autotrophic biofilms play a vital role in primary production and nutrient cycling in freshwater ecosystems but are increasingly exposed to chemical stressors such as antibiotics or herbicides. Although nutrient availability may modulate biofilm sensitivity, its impact on biofilm responses to these stressors remains poorly understood. In four independent experiments, we investigated the functional (ash-free dry weight and chlorophyll a, b and c) and structural (16S/18S rRNA metabarcoding) responses of stream-derived biofilms under low- and high-nutrient levels to chronic exposure (14 days) to the antibiotic ciprofloxacin and the herbicide propyzamide in laboratory stream microcosms. High-nutrient levels strongly increased biofilms functional responses and altered the community composition. Chemical exposure led to pronounced shifts in prokaryotic (ciprofloxacin) and eukaryotic (propyzamide) communities, but without significant effects on functional responses, suggesting functional redundancy and ecological buffering capacity of freshwater biofilms. These results highlight the critical role of nutrient supply in biofilm responses and the need for caution when extrapolating laboratory results to field conditions.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12481197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145130365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Within-host microbial selection and multiple microbial generations buffer the loss of host fitness under environmental change. 宿主内部的微生物选择和多代微生物缓冲了环境变化下宿主适应性的丧失。

IF 3.2 3区生物学

FEMS microbiology ecology Pub Date : 2025-09-22 DOI: 10.1093/femsec/fiaf089

William S Pearman, Allen G Rodrigo, Anna W Santure

{"title":"Within-host microbial selection and multiple microbial generations buffer the loss of host fitness under environmental change.","authors":"William S Pearman, Allen G Rodrigo, Anna W Santure","doi":"10.1093/femsec/fiaf089","DOIUrl":"10.1093/femsec/fiaf089","url":null,"abstract":"The relationship between, and joint selection on, a host and its microbes-the holobiont-can impact evolutionary and ecological outcomes of the host and its microbial community. We develop an agent-based modelling framework for understanding the ecological dynamics of hosts and their microbiomes. Our model incorporates numerous microbial generations per host generation allowing selection on both host and microbes. We then explore host and microbiome fitness and diversity in response to environmental change. We demonstrate that multiple microbial generations can buffer changes experienced across host lifetimes by smoothing environmental transitions. Our simulations reveal that microbial fitness and host fitness are at odds with each other when considering the impact of vertical inheritance of microbial communities from a host to its offspring-where high parent-offspring microbial transmission favours microbial fitness, while low transmission favours host fitness. These tradeoffs are minimized when microbial generation count per host generation is high. This may arise from 'cross-generational priority effects' which maintain diversity within the community and can subsequently enable selection of beneficial microbes by the host. Our model is extensible into new areas of holobiont research and provides novel insights into holobiont evolution under variable environmental conditions.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12451448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linking extreme light availability to cellular function in algae-dominated communities on the Greenland Ice Sheet. 将格陵兰冰盖上以藻类为主的群落的极端光可用性与细胞功能联系起来。

IF 3.2 3区生物学

FEMS microbiology ecology Pub Date : 2025-09-22 DOI: 10.1093/femsec/fiaf095

Helen K Feord, Christoph Keuschnig, Christopher B Trivedi, Rey Mourot, Athanasios Zervas, Thomas Turpin-Jelfs, Martyn Tranter, Alexandre M Anesio, Lorenz Adrian, Liane G Benning

{"title":"Linking extreme light availability to cellular function in algae-dominated communities on the Greenland Ice Sheet.","authors":"Helen K Feord, Christoph Keuschnig, Christopher B Trivedi, Rey Mourot, Athanasios Zervas, Thomas Turpin-Jelfs, Martyn Tranter, Alexandre M Anesio, Lorenz Adrian, Liane G Benning","doi":"10.1093/femsec/fiaf095","DOIUrl":"10.1093/femsec/fiaf095","url":null,"abstract":"Glacier ice algae of the streptophyte genus Ancylonema bloom on glaciers globally, including the Greenland Ice Sheet. These algae survive under extreme high light conditions in the summer, as well as under very low light or total darkness during (polar) winters and winter burial under snow. However, little is known about the cellular mechanisms underpinning glacier ice algae ecophysiological plasticity in response to extreme light availability. To address this knowledge gap, we evaluated the response of Ancylonema-dominated taxa in samples from the Greenland Ice Sheet to light and dark conditions during a 12-day period using combined multi-omics analyses. The microbial community was not substantially altered during the 12 days of dark incubation, however transcriptomic analysis demonstrated that the algae-associated heterotrophs became more active in the dark. In contrast, we identified a striking algal transcriptome stability in light conditions, in addition to high oxidative stress responses and evidence for high photosystem protein turnover. We also identified transcriptional reprogramming linked to sugar uptake and phytohormone signalling during dark incubation. These results provide crucial clues into the ability of glacier ice algae to adapt and survive in a harsh and extremely variable light environment.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12501423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145174406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Winter microbial community structure and methane-cycling potential in constructed agricultural wetlands across regions and microhabitats. 农业人工湿地冬季微生物群落结构与甲烷循环潜力

IF 3.2 3区生物学

FEMS microbiology ecology Pub Date : 2025-08-23 DOI: 10.1093/femsec/fiaf086

Tong Liu, Klara Li Yngve, Martyn Futter, Mike Peacock, John Strand, Stefan Bertilsson, Pia Geranmayeh

{"title":"Winter microbial community structure and methane-cycling potential in constructed agricultural wetlands across regions and microhabitats.","authors":"Tong Liu, Klara Li Yngve, Martyn Futter, Mike Peacock, John Strand, Stefan Bertilsson, Pia Geranmayeh","doi":"10.1093/femsec/fiaf086","DOIUrl":"10.1093/femsec/fiaf086","url":null,"abstract":"Constructed wetlands are widely used to reduce nutrient loading to downstream waters, but they can also emit methane, a potent greenhouse gas. This trade-off between water quality benefits and climate impacts is driven by microbial processes that remain poorly understood in winter. We examined microbial community composition and methane-cycling potential in surface water samples from constructed wetlands in two agricultural regions of Sweden during the winter season, focusing on the effects of emergent vegetation and environmental conditions. Western wetlands, characterized by higher total nitrogen and dissolved oxygen, exhibited significantly greater microbial diversity and more complex co-occurrence networks than eastern wetlands. At the phylum level, Actinobacteriota and Firmicutes were more abundant in the west, while Bacteroidota dominated the east. The effects of emergent vegetation were region-specific: in the west, vegetated zones supported higher diversity and enrichment of plant-associated taxa. Several taxa affiliated with methanotrophs showed higher relative abundance in vegetated zones of the western wetlands, suggesting vegetation may enhance methane oxidation potential in surface waters, even though methane concentrations were similar. Overall, winter microbial networks remained structured, emphasizing the need for integrated microbial and biogeochemical studies to guide wetland design features, such as vegetation and nutrient regimes, that support both methane mitigation and nutrient retention in cold-climate agricultural landscapes.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12421997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Friend or foe? Concentration of a commensal microbe induces distinct responses in developing honey bees exposed to field-realistic pesticide concentrations. 朋友还是敌人？一种共生微生物的浓度在暴露于田间实际杀虫剂浓度的发育中的蜜蜂中引起不同的反应。

IF 3.2 3区生物学

FEMS microbiology ecology Pub Date : 2025-08-23 DOI: 10.1093/femsec/fiaf080

Monika Yordanova, Xiao Zhang, Carlota B Torres, Sophie E F Evison, Richard J Gill, Peter Graystock

{"title":"Friend or foe? Concentration of a commensal microbe induces distinct responses in developing honey bees exposed to field-realistic pesticide concentrations.","authors":"Monika Yordanova, Xiao Zhang, Carlota B Torres, Sophie E F Evison, Richard J Gill, Peter Graystock","doi":"10.1093/femsec/fiaf080","DOIUrl":"10.1093/femsec/fiaf080","url":null,"abstract":"Commensal microbes play important roles in modulating host health through varied mechanisms. Enterococcus faecalis, a Gram-positive commensal bacterium found across a wide range of hosts, has the potential to benefit its host through probiotic, antimicrobial and detoxification properties. However, it can also cause adverse effects, disrupting the host's healthy microbial communities and responses to co-stressors. Its context-dependent impact on the health of the agriculturally important pollinator - Apis mellifera - has been sparsely explored. Here, we examined the effects on honey bee brood survivorship and development when exposed at different concentrations and when co-exposed with chemical stressors (acetamiprid, thymol, glyphosate, and a mixture of the three). We found high doses of E. faecalis significantly reduced larval survivorship and size of brood at multiple developmental stages. Conversely, we found that low doses of E. faecalis increased larval size when individuals were co-exposed to the pesticide mixture. We also found that glyphosate alone and the pesticide mixture reduced the mass of brown-eyed pupae. These results are the first to show the dual role of E. faecalis in honey bee health is dependent on the concentration of the microbe and the co-stressors that brood are exposed to.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12418955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plastic leachates alter the composition of marine microbial communities, not functional potential for plastic degradation. 塑料渗滤液改变了海洋微生物群落的组成，而不是塑料降解的功能潜力。

IF 3.2 3区生物学

FEMS microbiology ecology Pub Date : 2025-08-23 DOI: 10.1093/femsec/fiaf087

Stefan D M Maday, Kim M Handley, Grant Northcott, Joanne M Kingsbury, Dawn Smith, Olga Pantos, Gavin Lear

{"title":"Plastic leachates alter the composition of marine microbial communities, not functional potential for plastic degradation.","authors":"Stefan D M Maday, Kim M Handley, Grant Northcott, Joanne M Kingsbury, Dawn Smith, Olga Pantos, Gavin Lear","doi":"10.1093/femsec/fiaf087","DOIUrl":"10.1093/femsec/fiaf087","url":null,"abstract":"Plastics in the world's oceans are exposed to diverse environmental stressors that accelerate fragmentation and the leaching of associated additives. The impact of potentially toxic plastic degradation products and additives on marine microorganisms remains poorly understood. We assessed the impact of plastic leachate on marine microbial communities in vitro by exposure to one of four plastic leachates [from linear low-density polyethylene (LLPDE), polyamide-6 (or polycaprolactam; PA6), polyethylene terephthalate (PET), and polylactic acid (PLA)], prepared by immersing plastics in artificial seawater salts broth for three months at 80°C. Microbial communities were then exposed to different leachates. PLA-leachate-exposed communities differed significantly in composition from other plastic-leachate-exposed communities (PERMANOVA, P=0.001) as assessed by 16S rRNA gene and ITS region amplicon sequencing. Communities exposed to PLA leachate contained a higher proportion of Proteobacteria, specifically Halomonas spp. Greater relative abundances of Psathyrellaceae fungi also distinguished PLA-leachate communities. Despite significant differences in the structure of communities exposed to PLA leachate, we found no difference in the relative abundances of differentially expressed gene transcripts associated with known plastic degradation genes. While biodegradable plastics persist for shorter times in the environment than traditional plastics, our study indicates the potential for these plastic types to impact marine microbial communities.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":"101 9","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12445843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobiota vaccine induces lysine-mediated modulation of tick immunity affecting Borrelia colonization. 抗微生物疫苗诱导赖氨酸介导的影响伯氏疏螺旋体定植的蜱免疫调节。

IF 3.2 3区生物学

FEMS microbiology ecology Pub Date : 2025-08-23 DOI: 10.1093/femsec/fiaf082

Lourdes Mateos-Hernandez, Lianet Abuin-Denis, Alejandra Wu-Chuang, Apolline Maitre, Helena Roháčková, Ryan O M Rego, Elianne Piloto-Sardiñas, James Valdes, Stefania Porcelli, Aurelie Heckmann, Sara Moutailler, Covadonga Lucas-Torres, Martin Moos, Stanislav Opekar, Myriam Kratou, Dasiel Obregon, Alejandro Cabezas-Cruz

{"title":"Antimicrobiota vaccine induces lysine-mediated modulation of tick immunity affecting Borrelia colonization.","authors":"Lourdes Mateos-Hernandez, Lianet Abuin-Denis, Alejandra Wu-Chuang, Apolline Maitre, Helena Roháčková, Ryan O M Rego, Elianne Piloto-Sardiñas, James Valdes, Stefania Porcelli, Aurelie Heckmann, Sara Moutailler, Covadonga Lucas-Torres, Martin Moos, Stanislav Opekar, Myriam Kratou, Dasiel Obregon, Alejandro Cabezas-Cruz","doi":"10.1093/femsec/fiaf082","DOIUrl":"10.1093/femsec/fiaf082","url":null,"abstract":"Tick microbiota influences Borrelia colonization, but changes in the microbiota-derived metabolite and how this affects tick physiology and vector competence is unclear. We investigated whether microbiota-induced metabolite modifications influence tick physiology and pathogen transmission. Using an antimicrobiota vaccine (live Escherichia coli) to immunize mice, we generated host antibodies that modulated the tick microbiome, decreasing bacterial abundance and increasing lysine levels in ticks. Elevated lysine correlated with increased tick weight. Lysine supplementation experiments enhanced defensin expression with DefMT6 exhibiting anti-Borrelia activity, reducing pathogen load in ticks. Our findings demonstrate that antimicrobiota vaccines induce metabolite changes, affecting tick physiology, immunity, and vector competence. These insights open new avenues for developing microbiota-targeted strategies to control tick-borne diseases.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12374725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144855042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Landscape heterogeneity, forest structure, and mammalian host diversity shape tick density and prevalence of the causative agent of Lyme borreliosis. 景观异质性、森林结构和哺乳动物宿主多样性决定了莱姆病病原蜱虫密度和流行率。

IF 3.2 3区生物学

FEMS microbiology ecology Pub Date : 2025-08-23 DOI: 10.1093/femsec/fiaf088

Sara Weilage, Max Müller, Lara Maria Inge Heyse, Dana Rüster, Manfred Ayasse, Martin Pfeffer, Anna Obiegala

{"title":"Landscape heterogeneity, forest structure, and mammalian host diversity shape tick density and prevalence of the causative agent of Lyme borreliosis.","authors":"Sara Weilage, Max Müller, Lara Maria Inge Heyse, Dana Rüster, Manfred Ayasse, Martin Pfeffer, Anna Obiegala","doi":"10.1093/femsec/fiaf088","DOIUrl":"10.1093/femsec/fiaf088","url":null,"abstract":"Ticks, particularly Ixodes ricinus, and the associated Lyme borreliosis risk, represent key concerns within the One Health framework, prompting extensive research in this field. However, comprehensive studies that jointly consider landscape characteristics, local forest structure and management, climate, and host community composition-alongside direct measures of tick density and infection status with Borrelia spp., the bacterial agents causing Lyme borreliosis, are scarce. In this study, we test the hypothesis that habitat diversity exerts a dilution effect, primarily by supporting greater diversity of mammal hosts. Therefore, we examined I. ricinus tick density and Borrelia spp. prevalence in relation to a comprehensive set of habitat and host-related variables. Ticks were collected using the flagging method and mammal hosts were monitored using an innovative camera-trapping approach across 25 forest plots along a land-use gradient within the Schwäbische Alb exploratory in Germany. Both tick density and Borrelia spp. prevalence are influenced by a complex combination of habitat factors across different spatial scales, as well as the mammal host community composition. Overall, our results provide novel support to the dilution effect hypothesis, suggesting that greater habitat and host diversity contribute to a reduced Lyme borreliosis risk in this region.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":"101 9","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12445845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymers and immersion time shape bacterial pathogen and antibiotic resistance profiles in aquaculture facilities. 聚合物和浸泡时间影响水产养殖设施中细菌病原体和抗生素耐药性。

IF 3.2 3区生物学

FEMS microbiology ecology Pub Date : 2025-08-23 DOI: 10.1093/femsec/fiaf076

Jeanne Naudet, Jean-Christophe Auguet, Thierry Bouvier, Raherimino Rakotovao, Tony Motte, Loïc Gaumez, Tania Crucitti, Fabien Rieuvilleneuve, Emmanuelle Roque d'Orbcastel

{"title":"Polymers and immersion time shape bacterial pathogen and antibiotic resistance profiles in aquaculture facilities.","authors":"Jeanne Naudet, Jean-Christophe Auguet, Thierry Bouvier, Raherimino Rakotovao, Tony Motte, Loïc Gaumez, Tania Crucitti, Fabien Rieuvilleneuve, Emmanuelle Roque d'Orbcastel","doi":"10.1093/femsec/fiaf076","DOIUrl":"10.1093/femsec/fiaf076","url":null,"abstract":"Most equipment used in aquaculture farms is made of plastic. Plastics-associated biofilms may contain potential human pathogenic bacteria (PHPB) and antibiotic-resistant bacteria (ARB). Understanding the influence of farming practices on the biofouling development and composition is thus essential to control associated microbiological risks. We combined results from metabarcoding analyses, bacterial cultures, and antibiotic susceptibility testing to compare the bacterial pathobiome and resistome associated with plastic aquaculture equipment, including two polyamide nets and a polyester liner, with those associated to a hemp net and a glass control. Over the 3 months of incubation in an aquaculture farm, plastics exhibited neither higher levels of PHPB nor more multiple antibiotic resistance compared to other solid substrates, but they did present specific PHPB and ARB profiles. Bacterial members of the Vibrionaceae and Staphylococcaceae families were more abundant in plastic PHPB communities (respectively 47% and 22% of PHPB reads) than in other substrate ones (4% and 0.22% of PHPB reads). The plastic-associated antibiotic resistance profiles showed higher resistance against quinolones. These results suggest that aquaculture equipment could act as a reservoir for some PHPB and ARB, and that equipment composition and immersion time could be levers to control associated sanitary risks.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Depuration of a solitary ascidian depletes transient bacteria without altering microbiome alpha-diversity. 单独的海鞘的净化消耗瞬态细菌而不改变微生物组α多样性。

IF 3.2 3区生物学

FEMS microbiology ecology Pub Date : 2025-07-14 DOI: 10.1093/femsec/fiaf078

Brenna Hutchings, Susanna López-Legentil, Lauren M Stefaniak, Marie L Nydam, Patrick M Erwin

{"title":"Depuration of a solitary ascidian depletes transient bacteria without altering microbiome alpha-diversity.","authors":"Brenna Hutchings, Susanna López-Legentil, Lauren M Stefaniak, Marie L Nydam, Patrick M Erwin","doi":"10.1093/femsec/fiaf078","DOIUrl":"10.1093/femsec/fiaf078","url":null,"abstract":"Depuration, or the process of clearing impurities from the gut, is commonly applied to marine food products due to its efficacy in removing human pathogens from shellfish and edible ascidians. Recent studies also reported that depuration of filter-feeding animals helped reduce transient bacteria and identify resident symbionts in gut microbiome studies. Here, we examined the impact of depuration on bacteria in the branchial sac, gut, and hepatic gland of the solitary ascidian Pyura vittata. Replicates were kept in filtered seawater for 4 days prior to dissection (aquaria-depuration) and compared to samples that were immediately processed following collection (wild-no depuration) and replicates kept in unfiltered seawater for 4 days (aquaria-control). 16S rRNA gene sequence analysis revealed no significant differences among ascidian sources for microbial alpha-diversity but significant shifts in beta-diversity. Depuration reduced the number of core bacteria markedly (66%-84%) across all body regions, and bacteria that remained postdepuration consisted of genera associated with enhanced host health and resilience within other marine symbioses. Our results suggest that microbial profiles obtained following depuration do not substantially differ from those of nondepurated animals, but depuration can help differentiate transient from core and resident taxa in complex host-microbiome symbioses.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12345199/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144752828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0