FEMS microbiology ecology最新文献_第3页

Experimental evidence on the impact of climate-induced hydrological and thermal variations on glacier-fed stream biofilms. 气候引起的水文和热量变化对冰川溪流生物膜影响的实验证据。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2025-01-07 DOI: 10.1093/femsec/fiae163

David Touchette, Martina Gonzalez Mateu, Grégoire Michoud, Nicola Deluigi, Ramona Marasco, Daniele Daffonchio, Hannes Peter, Tom Battin

{"title":"Experimental evidence on the impact of climate-induced hydrological and thermal variations on glacier-fed stream biofilms.","authors":"David Touchette, Martina Gonzalez Mateu, Grégoire Michoud, Nicola Deluigi, Ramona Marasco, Daniele Daffonchio, Hannes Peter, Tom Battin","doi":"10.1093/femsec/fiae163","DOIUrl":"10.1093/femsec/fiae163","url":null,"abstract":"Climate change is predicted to alter the hydrological and thermal regimes of high-mountain streams, particularly glacier-fed streams. However, relatively little is known about how these environmental changes impact the microbial communities in glacier-fed streams. Here, we operated streamside flume mesocosms in the Swiss Alps, where benthic biofilms were grown under treatments simulating climate change. Treatments comprised four flow (natural, intermittent, stochastic, and constant) and two temperature (ambient streamwater and warming of +2°C) regimes. We monitored microbial biomass, diversity, community composition, and metabolic diversity in biofilms over 3 months. We found that community composition was largely influenced by successional dynamics independent of the treatments. While stochastic and constant flow regimes did not significantly affect community composition, droughts altered their composition in the intermittent regime, favouring drought-adapted bacteria and decreasing algal biomass. Concomitantly, warming decreased algal biomass and the abundance of some typical glacier-fed stream bacteria and eukaryotes, and stimulated heterotrophic metabolism overall. Our study provides experimental evidence towards potential and hitherto poorly considered impacts of climate change on benthic biofilms in glacier-fed streams.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extensive environmental survey of free-living amoebae and their elusive association with Mycobacterium bovis or Mycobacterium avium subsp. paratuberculosis. 自由生活变形虫的广泛环境调查及其与牛分枝杆菌或鸟分枝杆菌亚种的难以捉摸的联系。副结核。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2025-01-07 DOI: 10.1093/femsec/fiae164

Amélie Jessu, Thierry Cochard, Mélanie Burtin, Stéphanie Crapart, Vincent Delafont, Ascel Samba-Louaka, Franck Biet, Jean-Louis Moyen, Yann Héchard

{"title":"Extensive environmental survey of free-living amoebae and their elusive association with Mycobacterium bovis or Mycobacterium avium subsp. paratuberculosis.","authors":"Amélie Jessu, Thierry Cochard, Mélanie Burtin, Stéphanie Crapart, Vincent Delafont, Ascel Samba-Louaka, Franck Biet, Jean-Louis Moyen, Yann Héchard","doi":"10.1093/femsec/fiae164","DOIUrl":"10.1093/femsec/fiae164","url":null,"abstract":"Free-living amoebae (FLA) are described as environmental reservoirs for some bacteria able to resist their phagocytosis. In the environment, the fate of Mycobacterium bovis (Mbo) and Mycobacterium avium subsp. paratuberculosis (Map) responsible for bovine tuberculosis and paratuberculosis, respectively, remains poorly understood and is considered potentially problematic in the eradication and control of these diseases. We hypothesize that FLA may play a role in the persistence of Mbo and Map in the environment. In this study, 90 samples were collected from herds affected by one or both diseases to investigate the diversity of amoeba and their associated bacteria. Metabarcoding analyses revealed that Acanthamoeba, Copromyxa, Naegleria, and Vermamoeba were the most represented genera of FLA, with Pseudomonadota being the bacteria most commonly found associated with FLA. Although no Mbo and Map DNA were identified by sequencing, traces were detected by ddPCR (digital droplet PCR), specifically targeting these bacteria. In conclusion, we described a wide diversity of FLA and associated bacteria in this environment. It also suggests that Map and Mbo could be associated, even weakly, with FLA in the environment. However, this needs to be confirmed by detecting a highest amount of DNA and, if possible, cultivable Map and/or Mbo associated with these environmental FLA.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11707876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An interdisciplinary perspective of the built-environment microbiome. 建筑环境微生物组的跨学科视角。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2025-01-07 DOI: 10.1093/femsec/fiae166

John S McAlister, Michael J Blum, Yana Bromberg, Nina H Fefferman, Qiang He, Eric Lofgren, Debra L Miller, Courtney Schreiner, K Selcuk Candan, Heather Szabo-Rogers, J Michael Reed

{"title":"An interdisciplinary perspective of the built-environment microbiome.","authors":"John S McAlister, Michael J Blum, Yana Bromberg, Nina H Fefferman, Qiang He, Eric Lofgren, Debra L Miller, Courtney Schreiner, K Selcuk Candan, Heather Szabo-Rogers, J Michael Reed","doi":"10.1093/femsec/fiae166","DOIUrl":"10.1093/femsec/fiae166","url":null,"abstract":"The built environment provides an excellent setting for interdisciplinary research on the dynamics of microbial communities. The system is simplified compared to many natural settings, and to some extent the entire environment can be manipulated, from architectural design to materials use, air flow, human traffic, and capacity to disrupt microbial communities through cleaning. Here, we provide an overview of the ecology of the microbiome in the built environment. We address niche space and refugia, population, and community (metagenomic) dynamics, spatial ecology within a building, including the major microbial transmission mechanisms, as well as evolution. We also address landscape ecology, connecting microbiomes between physically separated buildings. At each stage, we pay particular attention to the actual and potential interface between disciplines, such as ecology, epidemiology, materials science, and human social behavior. We end by identifying some opportunities for future interdisciplinary research on the microbiome of the built environment.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11715626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142863690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A genome-scale metabolic reconstruction provides insight into the metabolism of the thermophilic bacterium Rhodothermus marinus. 基因组尺度的代谢重建提供了对嗜热细菌海洋红热菌代谢的深入了解。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2025-01-07 DOI: 10.1093/femsec/fiae167

Thordis Kristjansdottir, Gudmundur O Hreggvidsson, Elisabet Eik Gudmundsdottir, Snaedis H Bjornsdottir, Olafur H Fridjonsson, Sigmar Karl Stefansson, Eva Nordberg Karlsson, Justine Vanhalst, Birkir Reynisson, Steinn Gudmundsson

{"title":"A genome-scale metabolic reconstruction provides insight into the metabolism of the thermophilic bacterium Rhodothermus marinus.","authors":"Thordis Kristjansdottir, Gudmundur O Hreggvidsson, Elisabet Eik Gudmundsdottir, Snaedis H Bjornsdottir, Olafur H Fridjonsson, Sigmar Karl Stefansson, Eva Nordberg Karlsson, Justine Vanhalst, Birkir Reynisson, Steinn Gudmundsson","doi":"10.1093/femsec/fiae167","DOIUrl":"10.1093/femsec/fiae167","url":null,"abstract":"The thermophilic bacterium Rhodothermus marinus has mainly been studied for its thermostable enzymes. More recently, the potential of using the species as a cell factory and in biorefinery platforms has been explored, due to the elevated growth temperature, native production of compounds such as carotenoids and exopolysaccharides, the ability to grow on a wide range of carbon sources including polysaccharides, and available genetic tools. A comprehensive understanding of the metabolism of cell factories is important. Here, we report a genome-scale metabolic model of R. marinus DSM 4252T. Moreover, the genome of the genetically amenable R. marinus ISCaR-493 was sequenced and the analysis of the core genome indicated that the model could be used for both strains. Bioreactor growth data were obtained, used for constraining the model and the predicted and experimental growth rates were compared. The model correctly predicted the growth rates of both strains. During the reconstruction process, different aspects of the R. marinus metabolism were reviewed and subsequently, both cell densities and carotenoid production were investigated for strain ISCaR-493 under different growth conditions. Additionally, the dxs gene, which was not found in the R. marinus genomes, from Thermus thermophilus was cloned on a shuttle vector into strain ISCaR-493 resulting in a higher yield of carotenoids.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11730185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamics of fungal endophytic communities in bilberry (Vaccinium myrtillus L.) fruits through development is shaped by host phenolic compounds. 越桔果实发育过程中真菌内生群落的动态受寄主酚类化合物的影响。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2025-01-07 DOI: 10.1093/femsec/fiae168

Minh-Phuong Nguyen, Kaisa Lehosmaa, Françoise Martz, Janne J Koskimäki, Katalin Toth, Saija H K Ahonen, Hely Häggman, Anna-Maria Pirttilä

{"title":"Dynamics of fungal endophytic communities in bilberry (Vaccinium myrtillus L.) fruits through development is shaped by host phenolic compounds.","authors":"Minh-Phuong Nguyen, Kaisa Lehosmaa, Françoise Martz, Janne J Koskimäki, Katalin Toth, Saija H K Ahonen, Hely Häggman, Anna-Maria Pirttilä","doi":"10.1093/femsec/fiae168","DOIUrl":"10.1093/femsec/fiae168","url":null,"abstract":"The physical and chemical properties of wild berry fruits change dramatically during development, and the ripe berries host species-specific endophytic communities. However, the development of fungal endophytic communities during berry ripening is unknown. We studied bilberries (Vaccinium myrtillus L.), valuable natural resources in northern Europe and richest sources of phenolic compounds, to characterize dynamics of the fungal communities over fruit developmental stages (raw, veraison, and ripe). Our focus was to examine the changes in the fruit phenolic compounds associated with the fungal community structure using liquid chromatography-mass spectrometry for phenolic compounds and high-throughput sequencing technology targeting the internal transcribed spacer 2 ribosomal DNA region for endophytic fungi. We found that the fungal diversity increased with the ripening stages. The fungal profile changed dramatically through fruit development, and the veraison stage was a transition stage, where the core mycobiome of fruits changed. The fungal community structure and abundance of the most dominant genera in raw and ripe stages, Monilinia and Cladosporium, respectively, were driven by the bilberry phenolic profile. We conclude that sampling time, tissue age, and phenolic compounds play important roles in the development of fruit fungal community. Moreover, phenolic compounds could be the host's strategy to recruit beneficial microbes.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":"101 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11730187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of anthropogenic influences on a tropical lake ecosystem and its surrounding catchment: a case study of Lake Sentani. 人为影响对热带湖泊生态系统及其周边集水区的作用：以森塔尼湖为例。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2025-01-07 DOI: 10.1093/femsec/fiae162

Sulung Nomosatryo, Daniel Lipus, Alexander Bartholomäus, Cynthia Henny, Iwan Ridwansyah, Puguh Sujarta, Sizhong Yang, Dirk Wagner, Jens Kallmeyer

{"title":"The role of anthropogenic influences on a tropical lake ecosystem and its surrounding catchment: a case study of Lake Sentani.","authors":"Sulung Nomosatryo, Daniel Lipus, Alexander Bartholomäus, Cynthia Henny, Iwan Ridwansyah, Puguh Sujarta, Sizhong Yang, Dirk Wagner, Jens Kallmeyer","doi":"10.1093/femsec/fiae162","DOIUrl":"10.1093/femsec/fiae162","url":null,"abstract":"Lake Sentani is a tropical lake in Indonesia, consisting of four interconnected sub-basins of different water depths. While previous work has highlighted the impact of catchment composition on biogeochemical processes in Lake Sentani, little is currently known about the microbiological characteristics across this unique ecosystem. With recent population growth in this historically rural area, the anthropogenic impact on Lake Sentani and hence its microbial life is also increasing. Therefore, we aimed to explore the influence of environmental and anthropogenic factors on the microbial diversity of Lake Sentani. Here, we present a detailed microbiological evaluation of Lake Sentani, analyzing 49 different sites across the lake, its tributary rivers and their river mouths to assess diversity and community structure using 16S rRNA gene sequencing. Our results reveal distinct communities in lake and river sediments, supporting the observed geochemical differences. Taxonomic assessment showed the potential impact of anthropogenic pressure along the northern, urbanized shore, as river and river mouth samples revealed high abundances of Bacteroidota, Firmicutes, and Cyanobacteria, which could be attributed to pollution and eutrophication. In contrast, lake sediment communities were dominated by Thermodesulfovibrionia, Methanomethylicia, Bathyarchaeia, and Thermoplasmata, suggesting sulfate reducing, thermophilic, acidophilic bacteria and methanogenic archaea to play an important role in tropical lake systems. This study provides novel insights into ecological functions of tropical lakes and contributes to the optimization of management strategies of Lake Sentani, ensuring its holistic preservation in the future.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11707878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review on comparative analysis of marine and freshwater fish gut microbiomes: insights into environmental impact on gut microbiota. 海洋和淡水鱼肠道微生物群的比较分析综述：环境对肠道微生物群的影响。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2025-01-07 DOI: 10.1093/femsec/fiae169

Binoy Kumar Singh, Kushal Thakur, Hishani Kumari, Danish Mahajan, Dixit Sharma, Amit Kumar Sharma, Sunil Kumar, Birbal Singh, Pranay Punj Pankaj, Rakesh Kumar

{"title":"A review on comparative analysis of marine and freshwater fish gut microbiomes: insights into environmental impact on gut microbiota.","authors":"Binoy Kumar Singh, Kushal Thakur, Hishani Kumari, Danish Mahajan, Dixit Sharma, Amit Kumar Sharma, Sunil Kumar, Birbal Singh, Pranay Punj Pankaj, Rakesh Kumar","doi":"10.1093/femsec/fiae169","DOIUrl":"10.1093/femsec/fiae169","url":null,"abstract":"The gut microbiota, which includes prokaryotes, archaea, and eukaryotes such as yeasts, some protozoa, and fungi, significantly impacts fish by affecting digestion, metabolism, and the immune system. In this research, we combine various tasks carried out by various bacteria in the gut of fish. This study also examines the gut microbiome composition of marine and freshwater fish, identifying important bacterial species linked to different biological functions. The diversity within fish species highlights the importance of considering nutrition, habitat, and environmental factors in microbiological research on fish. The ever-changing gut microbiome of the fish indicates that microbial communities are specifically adapted to meet the needs of both the host and its environment. This indicates that the fish can adjust to a specific environment with the help of gut microbiota. This important research is crucial for comprehending the complex relationships between fish and their gut bacteria in different aquatic environments. These discoveries have implications for aquaculture practices, fisheries administration, and the broader ecological processes of both freshwater and marine environments. With further progress in this area of study, the knowledge acquired would offer a valuable standpoint to enhance our comprehension of aquatic microbiology and enhance the sustainability and nutrition of fish resources.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11730441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The type of environment has a greater impact on the larval microbiota of Anopheles arabiensis than on the microbiota of their breeding water. 环境类型对阿拉伯按蚊幼虫微生物群的影响大于对其繁殖水体微生物群的影响。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2025-01-07 DOI: 10.1093/femsec/fiae161

Lorenzo Assentato, Louise K J Nilsson, Carl Brunius, Vilhelm Feltelius, Rasmus Elleby, Richard J Hopkins, Olle Terenius

{"title":"The type of environment has a greater impact on the larval microbiota of Anopheles arabiensis than on the microbiota of their breeding water.","authors":"Lorenzo Assentato, Louise K J Nilsson, Carl Brunius, Vilhelm Feltelius, Rasmus Elleby, Richard J Hopkins, Olle Terenius","doi":"10.1093/femsec/fiae161","DOIUrl":"10.1093/femsec/fiae161","url":null,"abstract":"Mosquito larvae of the genus Anopheles develop entirely in water, frequently visiting the surface for air. The aquatic environment plays a key role in shaping their microbiota, but the connection between environmental characteristics of breeding sites and larval microbiota remains underexplored. This study focuses on Anopheles arabiensis, which inhabits the surface microlayer (SML) of breeding sites, a zone with high particle density. We hypothesized that the SML could allow us to capture the diversity of the surrounding environment, and in turn its influence on the larval microbial communities. To test this, we collected A. arabiensis larvae and SML samples from various breeding sites categorized by environmental features. Our results confirm that breeding site characteristics are significant drivers of the bacterial species present in mosquito larvae. Additionally, we found that the larval micro-environment selectively shapes its microbiota, highlighting a dynamic interplay between environmental and internal factors. Interestingly, specific bacterial families were associated with the presence or absence of larvae in breeding sites, suggesting potential ecological roles. These findings expand our understanding of vector-mosquito microbiota, emphasizing the importance of breeding site features in shaping larval microbial communities and providing a foundation for future research on mosquito ecology and control strategies.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142853381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and networking of uni-cyanobacterial cultures and associated heterotrophic bacteria from the benthic microbial mat of a desert hydrothermal spring. 沙漠热液泉底栖微生物垫中单青霉菌培养物和相关异养菌的多样性和网络化。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-11-23 DOI: 10.1093/femsec/fiae148

Khaoula Lassoued, Mouna Mahjoubi, Elias Asimakis, Naima Bel Mokhtar, Panagiota Stathopoulou, Refka Ben Hamouda, Olfa Bousselmi, Ramona Marasco, Ahmed Slaheddine Masmoudi, Daniele Daffonchio, George Tsiamis, Ameur Cherif

{"title":"Diversity and networking of uni-cyanobacterial cultures and associated heterotrophic bacteria from the benthic microbial mat of a desert hydrothermal spring.","authors":"Khaoula Lassoued, Mouna Mahjoubi, Elias Asimakis, Naima Bel Mokhtar, Panagiota Stathopoulou, Refka Ben Hamouda, Olfa Bousselmi, Ramona Marasco, Ahmed Slaheddine Masmoudi, Daniele Daffonchio, George Tsiamis, Ameur Cherif","doi":"10.1093/femsec/fiae148","DOIUrl":"10.1093/femsec/fiae148","url":null,"abstract":"Thermal springs harbour microorganisms, often dominated by cyanobacteria, which form biofilms and microbial mats. These phototrophic organisms release organic exudates into their immediate surroundings, attracting heterotrophic bacteria that contribute to the diversity and functioning of these ecosystems. In this study, the microbial mats from a hydrothermal pool in the Ksar Ghilane oasis in the Grand Erg Oriental of the Desert Tunisia were collected to obtain cyanobacterial cultures formed by single cyanobacterial species. High-throughput analysis showed that while the microbial mat hosted diverse cyanobacteria, laboratory cultures selectively enriched cyanobacteria from the Leptolyngbya, Nodosilinea, and Arthronema. Per each of these genera, multiple non-axenic uni-cyanobacterial cultures were established, totalling 41 cultures. Cyanobacteria taxa mediated the assembly of distinct heterotrophic bacterial communities, with members of the Proteobacteria and Actinobacteria phyla dominating. The bacterial communities of uni-cyanobacterial cultures were densely interconnected, with heterotrophic bacteria preferentially co-occurring with each other. Our study highlighted the complex structures of non-axenic uni-cyanobacterial cultures, where taxonomically distinct cyanobacteria consistently associate with specific groups of heterotrophic bacteria. The observed associations were likely driven by common selection pressures in the laboratory, such as cultivation conditions and specific hosts, and may not necessarily reflect the microbial dynamic occurring in the spring microbial mats.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11650863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling detoxifying symbiosis and dietary influence on the Southern green shield bug microbiota. 揭示解毒共生和饮食对南方绿盾蝽微生物群的影响

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-11-23 DOI: 10.1093/femsec/fiae150

Magda A Rogowska-van der Molen, Hristina V Savova, Elke A T Janssen, Theo van Alen, Silvia Coolen, Robert S Jansen, Cornelia U Welte

{"title":"Unveiling detoxifying symbiosis and dietary influence on the Southern green shield bug microbiota.","authors":"Magda A Rogowska-van der Molen, Hristina V Savova, Elke A T Janssen, Theo van Alen, Silvia Coolen, Robert S Jansen, Cornelia U Welte","doi":"10.1093/femsec/fiae150","DOIUrl":"10.1093/femsec/fiae150","url":null,"abstract":"The Southern green shield bug, Nezara viridula, is an invasive piercing and sucking pest insect that feeds on crops and poses a threat to global food production. Insects live in close relationships with microorganisms providing their host with unique capabilities, such as resistance to toxic plant metabolites. In this study, we investigated the resistance to and detoxification of the plant metabolite 3-nitropropionic acid (NPA) by core and transient members of the N. viridula microbial community. Microbial community members showed a different tolerance to the toxin and we determined that six out of eight strains detoxified NPA. Additionally, we determined that NPA might interfere with the biosynthesis and transport of l-leucine. Moreover, our study explored the influence of diet on the gut microbial composition of N. viridula, demonstrating that switching to a single-plant diet shifts the abundance of core microbes. In line with this, testing pairwise microbial interactions revealed that core microbiota members support each other and repress the growth of transient microorganisms. With this work, we provide novel insights into the factors shaping the insect gut microbial communities and demonstrate that N. viridula harbours many toxin-degrading bacteria that could support its resistance to plant defences.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0