FEMS microbiology ecology最新文献_第3页

Isolation and characterization of novel acetogenic Moorella strains for employment as potential thermophilic biocatalysts. 分离和鉴定新型醋酸菌株，将其用作潜在的嗜热生物催化剂。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-08-13 DOI: 10.1093/femsec/fiae109

Tim Böer, Lisa Engelhardt, Alina Lüschen, Lena Eysell, Hiroki Yoshida, Dominik Schneider, Largus T Angenent, Mirko Basen, Rolf Daniel, Anja Poehlein

{"title":"Isolation and characterization of novel acetogenic Moorella strains for employment as potential thermophilic biocatalysts.","authors":"Tim Böer, Lisa Engelhardt, Alina Lüschen, Lena Eysell, Hiroki Yoshida, Dominik Schneider, Largus T Angenent, Mirko Basen, Rolf Daniel, Anja Poehlein","doi":"10.1093/femsec/fiae109","DOIUrl":"10.1093/femsec/fiae109","url":null,"abstract":"Thermophilic acetogenic bacteria have attracted attention as promising candidates for biotechnological applications such as syngas fermentation, microbial electrosynthesis, and methanol conversion. Here, we aimed to isolate and characterize novel thermophilic acetogens from diverse environments. Enrichment of heterotrophic and autotrophic acetogens was monitored by 16S rRNA gene-based bacterial community analysis. Seven novel Moorella strains were isolated and characterized by genomic and physiological analyses. Two Moorella humiferrea isolates showed considerable differences during autotrophic growth. The M. humiferrea LNE isolate (DSM 117358) fermented carbon monoxide (CO) to acetate, while the M. humiferrea OCP isolate (DSM 117359) transformed CO to hydrogen and carbon dioxide (H2 + CO2), employing the water-gas shift reaction. Another carboxydotrophic hydrogenogenic Moorella strain was isolated from the covering soil of an active charcoal burning pile and proposed as the type strain (ACPsT) of the novel species Moorella carbonis (DSM 116161T and CCOS 2103T). The remaining four novel strains were affiliated with Moorella thermoacetica and showed, together with the type strain DSM 2955T, the production of small amounts of ethanol from H2 + CO2 in addition to acetate. The physiological analyses of the novel Moorella strains revealed isolate-specific differences that considerably increase the knowledge base on thermophilic acetogens for future applications.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11328732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Moderately thermostable GH1 β-glucosidases from hyperacidophilic archaeon Cuniculiplasma divulgatum S5. 来自超嗜酸性古菌 Cuniculiplasma divulgatum S5 的中等恒温 GH1 β-葡萄糖苷酶。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-08-13 DOI: 10.1093/femsec/fiae114

Anna N Khusnutdinova, Hai Tran, Saloni Devlekar, Marco A Distaso, Ilya V Kublanov, Tatiana Skarina, Peter Stogios, Alexei Savchenko, Manuel Ferrer, Olga V Golyshina, Alexander F Yakunin, Peter N Golyshin

{"title":"Moderately thermostable GH1 β-glucosidases from hyperacidophilic archaeon Cuniculiplasma divulgatum S5.","authors":"Anna N Khusnutdinova, Hai Tran, Saloni Devlekar, Marco A Distaso, Ilya V Kublanov, Tatiana Skarina, Peter Stogios, Alexei Savchenko, Manuel Ferrer, Olga V Golyshina, Alexander F Yakunin, Peter N Golyshin","doi":"10.1093/femsec/fiae114","DOIUrl":"10.1093/femsec/fiae114","url":null,"abstract":"Family GH1 glycosyl hydrolases are ubiquitous in prokaryotes and eukaryotes and are utilized in numerous industrial applications, including bioconversion of lignocelluloses. In this study, hyperacidophilic archaeon Cuniculiplasma divulgatum (S5T=JCM 30642T) was explored as a source of novel carbohydrate-active enzymes. The genome of C. divulgatum encodes three GH1 enzyme candidates, from which CIB12 and CIB13 were heterologously expressed and characterized. Phylogenetic analysis of CIB12 and CIB13 clustered them with β-glucosidases from genuinely thermophilic archaea including Thermoplasma acidophilum, Picrophilus torridus, Sulfolobus solfataricus, Pyrococcus furiosus, and Thermococcus kodakarensis. Purified enzymes showed maximal activities at pH 4.5-6.0 (CIB12) and 4.5-5.5 (CIB13) with optimal temperatures at 50°C, suggesting a high-temperature origin of Cuniculiplasma spp. ancestors. Crystal structures of both enzymes revealed a classical (α/β)8 TIM-barrel fold with the active site located inside the barrel close to the C-termini of β-strands including the catalytic residues Glu204 and Glu388 (CIB12), and Glu204 and Glu385 (CIB13). Both enzymes preferred cellobiose over lactose as substrates and were classified as cellobiohydrolases. Cellobiose addition increased the biomass yield of Cuniculiplasma cultures growing on peptides by 50%, suggesting that the cellobiohydrolases expand the carbon substrate range and hence environmental fitness of Cuniculiplasma.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11376072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141912372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epibiont communities on mussels in relation to parasitism and location in the rocky intertidal zone. 贻贝上的附生虫群落与寄生和潮间带岩石区位置的关系。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-08-13 DOI: 10.1093/femsec/fiae101

Katherine M Davis, Laura Wegener Parfrey, Christopher D G Harley, Keith Holmes, Olivia Schaefer, Alyssa-Lois Gehman

{"title":"Epibiont communities on mussels in relation to parasitism and location in the rocky intertidal zone.","authors":"Katherine M Davis, Laura Wegener Parfrey, Christopher D G Harley, Keith Holmes, Olivia Schaefer, Alyssa-Lois Gehman","doi":"10.1093/femsec/fiae101","DOIUrl":"https://doi.org/10.1093/femsec/fiae101","url":null,"abstract":"The factors shaping host-parasite interactions and epibiont communities in the variable rocky intertidal zone are poorly understood. California mussels, Mytilus californianus, are colonized by endolithic cyanobacterial parasites which erode the host shell. These cyanobacteria become mutualistic under certain abiotic conditions because shell erosion can protect mussels from thermal stress. How parasitic shell erosion affects or is affected by epibiotic microbial communities on mussel shells and the context dependency of these interactions is unknown. We used transplant experiments to characterize assemblages of epibiotic bacteria and endolithic parasites on mussel shells across intertidal elevation gradients. We hypothesized that living mussels, and associated epibacterial communities, could limit colonization and erosion by endolithic cyanobacteria compared to empty mussel shells. We hypothesized that shell erosion would be associated with compositional shifts in the epibacterial community and tidal elevation. We found that living mussels experienced less shell erosion than empty shells, demonstrating potential biotic regulation of endolithic parasites. Increased shell erosion was not associated with a distinct epibacterial community and was decoupled from the relative abundance of putatively endolithic taxa. Our findings suggest that epibacterial community structure is not directly impacted by the dynamic symbiosis between endolithic cyanobacteria and mussels throughout the rocky intertidal zone.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dissolved organic phosphorus bond-class utilization by Synechococcus. Synechococcus 对溶解有机磷键类的利用。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-08-13 DOI: 10.1093/femsec/fiae099

Emily M Waggoner, Kahina Djaoudi, Julia M Diaz, Solange Duhamel

{"title":"Dissolved organic phosphorus bond-class utilization by Synechococcus.","authors":"Emily M Waggoner, Kahina Djaoudi, Julia M Diaz, Solange Duhamel","doi":"10.1093/femsec/fiae099","DOIUrl":"10.1093/femsec/fiae099","url":null,"abstract":"Dissolved organic phosphorus (DOP) contains compounds with phosphoester, phosphoanhydride, and phosphorus-carbon bonds. While DOP holds significant nutritional value for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by Synechococcus strains from open and coastal oceans. Both strains exhibited comparable growth rates when provided phosphate, a phosphoanhydride [3-polyphosphate and 45-polyphosphate], or a DOP compound with both phosphoanhydride and phosphoester bonds (adenosine 5'-triphosphate). Growth rates on phosphoesters [glucose-6-phosphate, adenosine 5'-monophosphate, bis(4-methylumbelliferyl) phosphate] were variable, and neither strain grew on selected phosphorus-carbon compounds. Both strains hydrolyzed 3-polyphosphate, then adenosine 5'-triphosphate, and lastly adenosine 5'-monophosphate, exhibiting preferential enzymatic hydrolysis of phosphoanhydride bonds. The strains' exoproteomes contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5'-triphosphate under phosphate deficiency, suggests active mineralization of phosphoanhydride bonds by these exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity toward the phosphoanhydride 3-polyphosphate, with varying affinities between strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition and highlight varied growth and enzymatic responses to molecular diversity within DOP bond-classes, thereby expanding our understanding of microbially mediated DOP cycling in marine ecosystems.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11319936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiota and the Volatile Profile of Avian Nests Associate with Each Other and with the Intensity of Parasitism. 鸟巢的微生物群和挥发性特征与寄生虫寄生强度相互关联。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-07-24 DOI: 10.1093/femsec/fiae106

Mónica Mazorra-Alonso, Juan Manuel Peralta-Sánchez, Philipp Heeb, Staffan Jacob, Manuel Martin-Vivaldi, Manuel Martínez-Bueno, Rafael Núñez-Gómez, Oriol Sacristán-Soriano, Juan José Soler

{"title":"Microbiota and the Volatile Profile of Avian Nests Associate with Each Other and with the Intensity of Parasitism.","authors":"Mónica Mazorra-Alonso, Juan Manuel Peralta-Sánchez, Philipp Heeb, Staffan Jacob, Manuel Martin-Vivaldi, Manuel Martínez-Bueno, Rafael Núñez-Gómez, Oriol Sacristán-Soriano, Juan José Soler","doi":"10.1093/femsec/fiae106","DOIUrl":"https://doi.org/10.1093/femsec/fiae106","url":null,"abstract":"Bacteria have been suggested as being partially responsible for avian-nest odours and, thus, volatiles from their metabolism could influence the intensity of selection pressures due to parasites detecting olfactory cues of their hosts. Here, we tested this hypothesis by exploring intra- and interspecific variability in microbial environments, volatile profiles, and intensity of ectoparasitism by Carnus hemapterus in nests of ten avian species. As expected, we found that (i) alpha and beta diversity of microbial and volatile profiles associated to each other. Moreover, (ii) alpha diversity of bacteria and volatiles of nest environment, as well as some particular bacteria and volatiles, associated with intensity of parasitism at early and late stage of the nestling period. Finally, (iii) alpha diversity of the nest microbiota, as well as some particular bacteria and volatiles, was correlated to fledging success. When considering them together, the results support the expected links between microbial environment and nest odours in different bird species, and between microbial environment and both ectoparasitism intensity and fledging success. Relative abundances of particular volatiles and bacteria predicted ectoparasitism and/or fledging success. Future research should prioritize experimental approaches directed to determine the role of bacteria and volatiles in the outcomes of host-ectoparasite interactions.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141758031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial anabolic and catabolic utilization of hydrocarbons in deep subseafloor sediments of Guaymas Basin. 瓜伊马斯盆地深层海底沉积物中微生物对碳氢化合物的合成代谢和分解代谢利用。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae093

Toshiki Nagakura, Yuki Morono, Motoo Ito, Kai Mangelsdorf, Stefanie Pötz, Ellen Schnabel, Jens Kallmeyer

{"title":"Microbial anabolic and catabolic utilization of hydrocarbons in deep subseafloor sediments of Guaymas Basin.","authors":"Toshiki Nagakura, Yuki Morono, Motoo Ito, Kai Mangelsdorf, Stefanie Pötz, Ellen Schnabel, Jens Kallmeyer","doi":"10.1093/femsec/fiae093","DOIUrl":"10.1093/femsec/fiae093","url":null,"abstract":"Guaymas Basin, located in the Gulf of California, is a hydrothermally active marginal basin. Due to steep geothermal gradients and localized heating by sill intrusions, microbial substrates like short-chain fatty acids and hydrocarbons are abiotically produced from sedimentary organic matter at comparatively shallow depths. We analyzed the effect of hydrocarbons on uptake of hydrocarbons by microorganisms via nano-scale secondary ion mass spectrometry (NanoSIMS) and microbial sulfate reduction rates (SRR), using samples from two drill sites sampled by IODP Expedition 385 (U1545C and U1546D). These sites are in close proximity of each other (ca. 1 km) and have very similar sedimentology. Site U1546D experienced the intrusion of a sill that has since then thermally equilibrated with the surrounding sediment. Both sites currently have an identical geothermal gradient, despite their different thermal history. The localized heating by the sill led to thermal cracking of sedimentary organic matter and formation of potentially bioavailable organic substrates. There were low levels of hydrocarbon and nitrogen uptake in some samples from both sites, mostly in surficial samples. Hydrocarbon and methane additions stimulated SRR in near-seafloor samples from Site U1545C, while samples from Site U1546D reacted positively only on methane. Our data indicate the potential of microorganisms to metabolize hydrocarbons even in the deep subsurface of Guaymas Basin.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11250447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microeukaryote community coalescence strengthens community stability and elevates diversity. 微核生物群落的凝聚加强了群落的稳定性并提高了多样性。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae100

Máté Vass, Anna J Székely, Ulla Carlsson-Graner, Johan Wikner, Agneta Andersson

{"title":"Microeukaryote community coalescence strengthens community stability and elevates diversity.","authors":"Máté Vass, Anna J Székely, Ulla Carlsson-Graner, Johan Wikner, Agneta Andersson","doi":"10.1093/femsec/fiae100","DOIUrl":"10.1093/femsec/fiae100","url":null,"abstract":"Mixing of entire microbial communities represents a frequent, yet understudied phenomenon. Here, we mimicked estuarine condition in a microcosm experiment by mixing a freshwater river community with a brackish sea community and assessed the effects of both environmental and community coalescences induced by varying mixing processes on microeukaryotic communities. Signs of shifted community composition of coalesced communities towards the sea parent community suggest asymmetrical community coalescence outcome, which, in addition, was generally less impacted by environmental coalescence. Community stability, inferred from community cohesion, differed among river and sea parent communities, and increased following coalescence treatments. Generally, community coalescence increased alpha diversity and promoted competition from the introduction (or emergence) of additional (or rare) species. These competitive interactions in turn had community stabilizing effect as evidenced by the increased proportion of negative cohesion. The fate of microeukaryotes was influenced by mixing ratios and frequencies (i.e. one-time versus repeated coalescence). Namely, diatoms were negatively impacted by coalescence, while fungi, ciliates, and cercozoans were promoted to varying extents, depending on the mixing ratios of the parent communities. Our study suggests that the predictability of coalescence outcomes was greater when the sea parent community dominated the final community, and this predictability was further enhanced when communities collided repeatedly.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11287207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A common ericoid shrub modulates the diversity and structure of fungal communities across an arbuscular to ectomycorrhizal tree dominance gradient. 一种常见的啮齿类灌木调节了从树根到外生菌根树木优势梯度上真菌群落的多样性和结构。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae092

Alexander Polussa, Elisabeth B Ward, Mark A Bradford, Angela M Oliverio

{"title":"A common ericoid shrub modulates the diversity and structure of fungal communities across an arbuscular to ectomycorrhizal tree dominance gradient.","authors":"Alexander Polussa, Elisabeth B Ward, Mark A Bradford, Angela M Oliverio","doi":"10.1093/femsec/fiae092","DOIUrl":"10.1093/femsec/fiae092","url":null,"abstract":"Differences between arbuscular (AM) and ectomycorrhizal (EcM) trees strongly influence forest ecosystem processes, in part through their impact on saprotrophic fungal communities. Ericoid mycorrhizal (ErM) shrubs likely also impact saprotrophic communities given that they can shape nutrient cycling by slowing decomposition rates and intensifying nitrogen limitation. We investigated the depth distributions of saprotrophic and EcM fungal communities in paired subplots with and without a common understory ErM shrub, mountain laurel (Kalmia latifolia L.), across an AM to EcM tree dominance gradient in a temperate forest by analyzing soils from the organic, upper mineral (0-10 cm), and lower mineral (cumulative depth of 30 cm) horizons. The presence of K. latifolia was strongly associated with the taxonomic and functional composition of saprotrophic and EcM communities. Saprotrophic richness was consistently lower in the Oa horizon when this ErM shrub species was present. However, in AM tree-dominated plots, the presence of the ErM shrub was associated with a higher relative abundance of saprotrophs. Given that EcM trees suppress both the diversity and relative abundance of saprotrophic communities, our results suggest that separate consideration of ErM shrubs and EcM trees may be necessary when assessing the impacts of plant mycorrhizal associations on belowground communities.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11250453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141456184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of turbulence on diatoms of the genus Pseudo-nitzschia spp. and associated bacteria. 湍流对假尼氏硅藻属硅藻及相关细菌的影响

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae094

Yanis Maire, François G Schmitt, Konstantinos Kormas, Sotirios Vasileiadis, Amandine Caruana, Dimitra-Ioli Skouroliakou, Vasileios Bampouris, Lucie Courcot, Fabienne Hervé, Muriel Crouvoisier, Urania Christaki

{"title":"Effects of turbulence on diatoms of the genus Pseudo-nitzschia spp. and associated bacteria.","authors":"Yanis Maire, François G Schmitt, Konstantinos Kormas, Sotirios Vasileiadis, Amandine Caruana, Dimitra-Ioli Skouroliakou, Vasileios Bampouris, Lucie Courcot, Fabienne Hervé, Muriel Crouvoisier, Urania Christaki","doi":"10.1093/femsec/fiae094","DOIUrl":"10.1093/femsec/fiae094","url":null,"abstract":"Turbulence is one of the least investigated environmental factors impacting the ecophysiology of phytoplankton, both at the community and individual species level. Here, we investigated, for the first time, the effect of a turbulence gradient (Reynolds number, from Reλ = 0 to Reλ = 360) on two species of the marine diatom Pseudo-nitzschia and their associated bacterial communities under laboratory conditions. Cell abundance, domoic acid (DA) production, chain formation, and Chl a content of P. fraudulenta and P. multiseries were higher for intermediate turbulence (Reλ = 160 or 240). DA was detectable only in P. multiseries samples. These observations were supported by transcriptomic analyses results, which suggested the turbulence related induction of the expression of the DA production locus, with a linkage to an increased photosynthetic activity of the total metatranscriptome. This study also highlighted a higher richness of the bacterial community associated with the nontoxic strain of P. fraudulenta in comparison to the toxic strain of P. multiseries. Bacillus was an important genus in P. multiseries cultures (relative abundance 15.5%) and its highest abundances coincided with the highest DA levels. However, associated bacterial communities of both Pseudo-nitzschia species did not show clear patterns relative to turbulence intensity.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11264304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of successive summer drought periods on bacterial diversity along a plant species richness gradient. 夏季连续干旱期对植物物种丰富度梯度上细菌多样性的影响

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae096

Yuri Pinheiro Alves de Souza, Roberto Siani, Cynthia Albracht, Yuanyuan Huang, Nico Eisenhauer, Anja Vogel, Cameron Wagg, Michael Schloter, Stefanie Schulz

{"title":"The effect of successive summer drought periods on bacterial diversity along a plant species richness gradient.","authors":"Yuri Pinheiro Alves de Souza, Roberto Siani, Cynthia Albracht, Yuanyuan Huang, Nico Eisenhauer, Anja Vogel, Cameron Wagg, Michael Schloter, Stefanie Schulz","doi":"10.1093/femsec/fiae096","DOIUrl":"10.1093/femsec/fiae096","url":null,"abstract":"Drought is a major stressor to soil microbial communities, and the intensification of climate change is predicted to increase hydric stress worldwide in the coming decades. As a possible mitigating factor for the consequences of prolonged drought periods, above and belowground biodiversity can increase ecosystem resistance and resilience by improving metabolic redundancy and complementarity as biodiversity increases. Here, we investigated the interaction effect between plant richness and successive, simulated summer drought on soil microbial communities during a period of 9 years.To do that, we made use of a well-established biodiversity experiment (The Jena Experiment) to investigate the response of microbial richness and community composition to successive drought periods alongside a plant richness gradient, which covers 1-, 2-, 4-, 8-, 16-, and 60-species plant communities. Plots were covered from natural precipitation by installing rain shelters 6 weeks every summer. Bulk soil samples were collected 1 year after the last summer drought was simulated. Our data indicate that bacterial richness increased after successive exposure to drought, with the increase being stable along the plant richness gradient. We identified a significant effect of plant species richness on the soil microbial community composition and determined the taxa significantly impacted by drought at each plant richness level. Our data successfully demonstrates that summer drought might have a legacy effect on soil bacterial communities.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11264299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0