FEMS microbiology ecology最新文献_第5页

Dissolved organic phosphorus bond-class utilization by Synechococcus. Synechococcus 对溶解有机磷键类的利用。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-08-13 DOI: 10.1093/femsec/fiae099

Emily M Waggoner, Kahina Djaoudi, Julia M Diaz, Solange Duhamel

{"title":"Dissolved organic phosphorus bond-class utilization by Synechococcus.","authors":"Emily M Waggoner, Kahina Djaoudi, Julia M Diaz, Solange Duhamel","doi":"10.1093/femsec/fiae099","DOIUrl":"10.1093/femsec/fiae099","url":null,"abstract":"Dissolved organic phosphorus (DOP) contains compounds with phosphoester, phosphoanhydride, and phosphorus-carbon bonds. While DOP holds significant nutritional value for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by Synechococcus strains from open and coastal oceans. Both strains exhibited comparable growth rates when provided phosphate, a phosphoanhydride [3-polyphosphate and 45-polyphosphate], or a DOP compound with both phosphoanhydride and phosphoester bonds (adenosine 5'-triphosphate). Growth rates on phosphoesters [glucose-6-phosphate, adenosine 5'-monophosphate, bis(4-methylumbelliferyl) phosphate] were variable, and neither strain grew on selected phosphorus-carbon compounds. Both strains hydrolyzed 3-polyphosphate, then adenosine 5'-triphosphate, and lastly adenosine 5'-monophosphate, exhibiting preferential enzymatic hydrolysis of phosphoanhydride bonds. The strains' exoproteomes contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5'-triphosphate under phosphate deficiency, suggests active mineralization of phosphoanhydride bonds by these exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity toward the phosphoanhydride 3-polyphosphate, with varying affinities between strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition and highlight varied growth and enzymatic responses to molecular diversity within DOP bond-classes, thereby expanding our understanding of microbially mediated DOP cycling in marine ecosystems.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11319936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Total RNA analysis of the active microbiome on moving bed biofilm reactor carriers under incrementally increasing micropollutant concentrations. 在微污染物浓度逐渐增加的情况下，对移动床生物膜反应器载体上的活性微生物群进行总 RNA 分析。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-08-13 DOI: 10.1093/femsec/fiae098

Joseph Donald Martin, Selina Tisler, Maria Scheel, Sif Svendsen, Muhammad Zohaib Anwar, Athanasios Zervas, Flemming Ekelund, Kai Bester, Lars Hestbjerg Hansen, Carsten Suhr Jacobsen, Lea Ellegaard-Jensen

{"title":"Total RNA analysis of the active microbiome on moving bed biofilm reactor carriers under incrementally increasing micropollutant concentrations.","authors":"Joseph Donald Martin, Selina Tisler, Maria Scheel, Sif Svendsen, Muhammad Zohaib Anwar, Athanasios Zervas, Flemming Ekelund, Kai Bester, Lars Hestbjerg Hansen, Carsten Suhr Jacobsen, Lea Ellegaard-Jensen","doi":"10.1093/femsec/fiae098","DOIUrl":"10.1093/femsec/fiae098","url":null,"abstract":"Micropollutants are increasingly prevalent in the aquatic environment. A major part of these originates from wastewater treatment plants since traditional treatment technologies do not remove micropollutants sufficiently. Moving bed biofilm reactors (MBBRs), however, have been shown to aid in micropollutant removal when applied to conventional wastewater treatment as a polishing step. Here, we used Total RNA sequencing to investigate both the active microbial community and functional dynamics of MBBR biofilms when these were exposed to increasing micropollutant concentrations over time. Concurrently, we conducted batch culture experiments using biofilm carriers from the MBBRs to assess micropollutant degradation potential. Our study showed that biofilm eukaryotes, in particular protozoa, were negatively influenced by micropollutant exposure, in contrast to prokaryotes that increased in relative abundance. Further, we found several functional genes that were differentially expressed between the MBBR with added micropollutants and the control. These include genes involved in aromatic and xenobiotic compound degradation. Moreover, the biofilm carrier batch experiment showed vastly different alterations in benzotriazole and diclofenac degradation following the increased micropollutant concentrations in the MBBR. Ultimately, this study provides essential insights into the microbial community and functional dynamics of MBBRs and how an increased load of micropollutants influences these dynamics.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11385203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial anabolic and catabolic utilization of hydrocarbons in deep subseafloor sediments of Guaymas Basin. 瓜伊马斯盆地深层海底沉积物中微生物对碳氢化合物的合成代谢和分解代谢利用。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae093

Toshiki Nagakura, Yuki Morono, Motoo Ito, Kai Mangelsdorf, Stefanie Pötz, Ellen Schnabel, Jens Kallmeyer

{"title":"Microbial anabolic and catabolic utilization of hydrocarbons in deep subseafloor sediments of Guaymas Basin.","authors":"Toshiki Nagakura, Yuki Morono, Motoo Ito, Kai Mangelsdorf, Stefanie Pötz, Ellen Schnabel, Jens Kallmeyer","doi":"10.1093/femsec/fiae093","DOIUrl":"10.1093/femsec/fiae093","url":null,"abstract":"Guaymas Basin, located in the Gulf of California, is a hydrothermally active marginal basin. Due to steep geothermal gradients and localized heating by sill intrusions, microbial substrates like short-chain fatty acids and hydrocarbons are abiotically produced from sedimentary organic matter at comparatively shallow depths. We analyzed the effect of hydrocarbons on uptake of hydrocarbons by microorganisms via nano-scale secondary ion mass spectrometry (NanoSIMS) and microbial sulfate reduction rates (SRR), using samples from two drill sites sampled by IODP Expedition 385 (U1545C and U1546D). These sites are in close proximity of each other (ca. 1 km) and have very similar sedimentology. Site U1546D experienced the intrusion of a sill that has since then thermally equilibrated with the surrounding sediment. Both sites currently have an identical geothermal gradient, despite their different thermal history. The localized heating by the sill led to thermal cracking of sedimentary organic matter and formation of potentially bioavailable organic substrates. There were low levels of hydrocarbon and nitrogen uptake in some samples from both sites, mostly in surficial samples. Hydrocarbon and methane additions stimulated SRR in near-seafloor samples from Site U1545C, while samples from Site U1546D reacted positively only on methane. Our data indicate the potential of microorganisms to metabolize hydrocarbons even in the deep subsurface of Guaymas Basin.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11250447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microeukaryote community coalescence strengthens community stability and elevates diversity. 微核生物群落的凝聚加强了群落的稳定性并提高了多样性。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae100

Máté Vass, Anna J Székely, Ulla Carlsson-Graner, Johan Wikner, Agneta Andersson

{"title":"Microeukaryote community coalescence strengthens community stability and elevates diversity.","authors":"Máté Vass, Anna J Székely, Ulla Carlsson-Graner, Johan Wikner, Agneta Andersson","doi":"10.1093/femsec/fiae100","DOIUrl":"10.1093/femsec/fiae100","url":null,"abstract":"Mixing of entire microbial communities represents a frequent, yet understudied phenomenon. Here, we mimicked estuarine condition in a microcosm experiment by mixing a freshwater river community with a brackish sea community and assessed the effects of both environmental and community coalescences induced by varying mixing processes on microeukaryotic communities. Signs of shifted community composition of coalesced communities towards the sea parent community suggest asymmetrical community coalescence outcome, which, in addition, was generally less impacted by environmental coalescence. Community stability, inferred from community cohesion, differed among river and sea parent communities, and increased following coalescence treatments. Generally, community coalescence increased alpha diversity and promoted competition from the introduction (or emergence) of additional (or rare) species. These competitive interactions in turn had community stabilizing effect as evidenced by the increased proportion of negative cohesion. The fate of microeukaryotes was influenced by mixing ratios and frequencies (i.e. one-time versus repeated coalescence). Namely, diatoms were negatively impacted by coalescence, while fungi, ciliates, and cercozoans were promoted to varying extents, depending on the mixing ratios of the parent communities. Our study suggests that the predictability of coalescence outcomes was greater when the sea parent community dominated the final community, and this predictability was further enhanced when communities collided repeatedly.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11287207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A common ericoid shrub modulates the diversity and structure of fungal communities across an arbuscular to ectomycorrhizal tree dominance gradient. 一种常见的啮齿类灌木调节了从树根到外生菌根树木优势梯度上真菌群落的多样性和结构。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae092

Alexander Polussa, Elisabeth B Ward, Mark A Bradford, Angela M Oliverio

{"title":"A common ericoid shrub modulates the diversity and structure of fungal communities across an arbuscular to ectomycorrhizal tree dominance gradient.","authors":"Alexander Polussa, Elisabeth B Ward, Mark A Bradford, Angela M Oliverio","doi":"10.1093/femsec/fiae092","DOIUrl":"10.1093/femsec/fiae092","url":null,"abstract":"Differences between arbuscular (AM) and ectomycorrhizal (EcM) trees strongly influence forest ecosystem processes, in part through their impact on saprotrophic fungal communities. Ericoid mycorrhizal (ErM) shrubs likely also impact saprotrophic communities given that they can shape nutrient cycling by slowing decomposition rates and intensifying nitrogen limitation. We investigated the depth distributions of saprotrophic and EcM fungal communities in paired subplots with and without a common understory ErM shrub, mountain laurel (Kalmia latifolia L.), across an AM to EcM tree dominance gradient in a temperate forest by analyzing soils from the organic, upper mineral (0-10 cm), and lower mineral (cumulative depth of 30 cm) horizons. The presence of K. latifolia was strongly associated with the taxonomic and functional composition of saprotrophic and EcM communities. Saprotrophic richness was consistently lower in the Oa horizon when this ErM shrub species was present. However, in AM tree-dominated plots, the presence of the ErM shrub was associated with a higher relative abundance of saprotrophs. Given that EcM trees suppress both the diversity and relative abundance of saprotrophic communities, our results suggest that separate consideration of ErM shrubs and EcM trees may be necessary when assessing the impacts of plant mycorrhizal associations on belowground communities.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11250453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141456184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Substrate complexity buffers negative interactions in a synthetic community of leaf litter degraders. 底物的复杂性可缓冲枯落叶降解生物合成群落中的负面相互作用。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae102

Parmis Abdoli, Clément Vulin, Miriam Lepiz, Alexander B Chase, Claudia Weihe, Alejandra Rodríguez-Verdugo

{"title":"Substrate complexity buffers negative interactions in a synthetic community of leaf litter degraders.","authors":"Parmis Abdoli, Clément Vulin, Miriam Lepiz, Alexander B Chase, Claudia Weihe, Alejandra Rodríguez-Verdugo","doi":"10.1093/femsec/fiae102","DOIUrl":"10.1093/femsec/fiae102","url":null,"abstract":"Leaf litter microbes collectively degrade plant polysaccharides, influencing land-atmosphere carbon exchange. An open question is how substrate complexity-defined as the structure of the saccharide and the amount of external processing by extracellular enzymes-influences species interactions. We tested the hypothesis that monosaccharides (i.e. xylose) promote negative interactions through resource competition, and polysaccharides (i.e. xylan) promote neutral or positive interactions through resource partitioning or synergism among extracellular enzymes. We assembled a three-species community of leaf litter-degrading bacteria isolated from a grassland site in Southern California. In the polysaccharide xylan, pairs of species stably coexisted and grew equally in coculture and in monoculture. Conversely, in the monosaccharide xylose, competitive exclusion and negative interactions prevailed. These pairwise dynamics remained consistent in a three-species community: all three species coexisted in xylan, while only two species coexisted in xylose, with one species capable of using peptone. A mathematical model showed that in xylose these dynamics could be explained by resource competition. Instead, the model could not predict the coexistence patterns in xylan, suggesting other interactions exist during biopolymer degradation. Overall, our study shows that substrate complexity influences species interactions and patterns of coexistence in a synthetic microbial community of leaf litter degraders.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11289631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141633114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of turbulence on diatoms of the genus Pseudo-nitzschia spp. and associated bacteria. 湍流对假尼氏硅藻属硅藻及相关细菌的影响

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae094

Yanis Maire, François G Schmitt, Konstantinos Kormas, Sotirios Vasileiadis, Amandine Caruana, Dimitra-Ioli Skouroliakou, Vasileios Bampouris, Lucie Courcot, Fabienne Hervé, Muriel Crouvoisier, Urania Christaki

{"title":"Effects of turbulence on diatoms of the genus Pseudo-nitzschia spp. and associated bacteria.","authors":"Yanis Maire, François G Schmitt, Konstantinos Kormas, Sotirios Vasileiadis, Amandine Caruana, Dimitra-Ioli Skouroliakou, Vasileios Bampouris, Lucie Courcot, Fabienne Hervé, Muriel Crouvoisier, Urania Christaki","doi":"10.1093/femsec/fiae094","DOIUrl":"10.1093/femsec/fiae094","url":null,"abstract":"Turbulence is one of the least investigated environmental factors impacting the ecophysiology of phytoplankton, both at the community and individual species level. Here, we investigated, for the first time, the effect of a turbulence gradient (Reynolds number, from Reλ = 0 to Reλ = 360) on two species of the marine diatom Pseudo-nitzschia and their associated bacterial communities under laboratory conditions. Cell abundance, domoic acid (DA) production, chain formation, and Chl a content of P. fraudulenta and P. multiseries were higher for intermediate turbulence (Reλ = 160 or 240). DA was detectable only in P. multiseries samples. These observations were supported by transcriptomic analyses results, which suggested the turbulence related induction of the expression of the DA production locus, with a linkage to an increased photosynthetic activity of the total metatranscriptome. This study also highlighted a higher richness of the bacterial community associated with the nontoxic strain of P. fraudulenta in comparison to the toxic strain of P. multiseries. Bacillus was an important genus in P. multiseries cultures (relative abundance 15.5%) and its highest abundances coincided with the highest DA levels. However, associated bacterial communities of both Pseudo-nitzschia species did not show clear patterns relative to turbulence intensity.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11264304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of successive summer drought periods on bacterial diversity along a plant species richness gradient. 夏季连续干旱期对植物物种丰富度梯度上细菌多样性的影响

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae096

Yuri Pinheiro Alves de Souza, Roberto Siani, Cynthia Albracht, Yuanyuan Huang, Nico Eisenhauer, Anja Vogel, Cameron Wagg, Michael Schloter, Stefanie Schulz

{"title":"The effect of successive summer drought periods on bacterial diversity along a plant species richness gradient.","authors":"Yuri Pinheiro Alves de Souza, Roberto Siani, Cynthia Albracht, Yuanyuan Huang, Nico Eisenhauer, Anja Vogel, Cameron Wagg, Michael Schloter, Stefanie Schulz","doi":"10.1093/femsec/fiae096","DOIUrl":"10.1093/femsec/fiae096","url":null,"abstract":"Drought is a major stressor to soil microbial communities, and the intensification of climate change is predicted to increase hydric stress worldwide in the coming decades. As a possible mitigating factor for the consequences of prolonged drought periods, above and belowground biodiversity can increase ecosystem resistance and resilience by improving metabolic redundancy and complementarity as biodiversity increases. Here, we investigated the interaction effect between plant richness and successive, simulated summer drought on soil microbial communities during a period of 9 years.To do that, we made use of a well-established biodiversity experiment (The Jena Experiment) to investigate the response of microbial richness and community composition to successive drought periods alongside a plant richness gradient, which covers 1-, 2-, 4-, 8-, 16-, and 60-species plant communities. Plots were covered from natural precipitation by installing rain shelters 6 weeks every summer. Bulk soil samples were collected 1 year after the last summer drought was simulated. Our data indicate that bacterial richness increased after successive exposure to drought, with the increase being stable along the plant richness gradient. We identified a significant effect of plant species richness on the soil microbial community composition and determined the taxa significantly impacted by drought at each plant richness level. Our data successfully demonstrates that summer drought might have a legacy effect on soil bacterial communities.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11264299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial: thematic issue on microbial ecotoxicology. 编辑：微生物生态毒理学专题。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae097

Stéphane Vuilleumier, Lise Barthelmebs, Natàlia Corcoll, Marina Hery, Dimitrios G Karpouzas, Lukas Y Wick

引用次数: 0

Spatio-temporal changes of small protist and free-living bacterial communities in a temperate dimictic lake: insights from metabarcoding and machine learning. 温带二叠纪湖泊中小型原生生物和自由生活细菌群落的时空变化：代谢标码和机器学习的启示。

IF 3.5 3区生物学

FEMS microbiology ecology Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae104

Michał Karlicki, Anna Bednarska, Paweł Hałakuc, Kacper Maciszewski, Anna Karnkowska

{"title":"Spatio-temporal changes of small protist and free-living bacterial communities in a temperate dimictic lake: insights from metabarcoding and machine learning.","authors":"Michał Karlicki, Anna Bednarska, Paweł Hałakuc, Kacper Maciszewski, Anna Karnkowska","doi":"10.1093/femsec/fiae104","DOIUrl":"10.1093/femsec/fiae104","url":null,"abstract":"Microbial communities, which include prokaryotes and protists, play an important role in aquatic ecosystems and influence ecological processes. To understand these communities, metabarcoding provides a powerful tool to assess their taxonomic composition and track spatio-temporal dynamics in both marine and freshwater environments. While marine ecosystems have been extensively studied, there is a notable research gap in understanding eukaryotic microbial communities in temperate lakes. Our study addresses this gap by investigating the free-living bacteria and small protist communities in Lake Roś (Poland), a dimictic temperate lake. Metabarcoding analysis revealed that both the bacterial and protist communities exhibit distinct seasonal patterns that are not necessarily shaped by dominant taxa. Furthermore, machine learning and statistical methods identified crucial amplicon sequence variants (ASVs) specific to each season. In addition, we identified a distinct community in the anoxic hypolimnion. We have also shown that the key factors shaping the composition of analysed community are temperature, oxygen, and silicon concentration. Understanding these community structures and the underlying factors is important in the context of climate change potentially impacting mixing patterns and leading to prolonged stratification.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11302952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0