Extremophiles最新文献_第6页

Comparative genomics reveals probable adaptations for xylose use in Thermoanaerobacterium saccharolyticum. 比较基因组学揭示了糖化热杆菌利用木糖的可能适应性。

IF 2.9 3区生物学

Extremophiles Pub Date : 2024-01-08 DOI: 10.1007/s00792-023-01327-x

Mateus Bernabe Fiamenghi, Juliana Silveira Prodonoff, Guilherme Borelli, Marcelo Falsarella Carazzolle, Gonçalo Amarante Guimaraes Pereira, Juliana José

{"title":"Comparative genomics reveals probable adaptations for xylose use in Thermoanaerobacterium saccharolyticum.","authors":"Mateus Bernabe Fiamenghi, Juliana Silveira Prodonoff, Guilherme Borelli, Marcelo Falsarella Carazzolle, Gonçalo Amarante Guimaraes Pereira, Juliana José","doi":"10.1007/s00792-023-01327-x","DOIUrl":"10.1007/s00792-023-01327-x","url":null,"abstract":"Second-generation ethanol, a promising biofuel for reducing greenhouse gas emissions, faces challenges due to the inefficient metabolism of xylose, a pentose sugar. Overcoming this hurdle requires exploration of genes, pathways, and organisms capable of fermenting xylose. Thermoanaerobacterium saccharolyticum is an organism capable of naturally fermenting compounds of industrial interest, such as xylose, and understanding evolutionary adaptations may help to bring novel genes and information that can be used for industrial yeast, increasing production of current bio-platforms. This study presents a deep evolutionary study of members of the firmicutes clade, focusing on adaptations in Thermoanaerobacterium saccharolyticum that may be related to overall fermentation metabolism, especially for xylose fermentation. One highlight is the finding of positive selection on a xylose-binding protein of the xylFGH operon, close to the annotated sugar binding site, with this protein already being found to be expressed in xylose fermenting conditions in a previous study. Results from this study can serve as basis for searching for candidate genes to use in industrial strains or to improve Thermoanaerobacterium saccharolyticum as a new microbial cell factory, which may help to solve current problems found in the biofuels' industry.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 1","pages":"9"},"PeriodicalIF":2.9,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139377360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metataxonomy of acid mine drainage microbiomes from the Santa Catarina Carboniferous Basin (Southern Brazil). 圣卡塔琳娜石炭纪盆地（巴西南部）酸性矿井排水微生物组元分类学。

IF 2.9 3区生物学

Extremophiles Pub Date : 2023-12-22 DOI: 10.1007/s00792-023-01324-0

Estácio Jussie Odisi, Robert Cardoso de Freitas, Diego Serrasol do Amaral, Saulo Britto da Silva, Marcus Adonai Castro da Silva, William de Oliveira Sant Ana, André Oliveira de Souza Lima, Leonardo Rubi Rörig

{"title":"Metataxonomy of acid mine drainage microbiomes from the Santa Catarina Carboniferous Basin (Southern Brazil).","authors":"Estácio Jussie Odisi, Robert Cardoso de Freitas, Diego Serrasol do Amaral, Saulo Britto da Silva, Marcus Adonai Castro da Silva, William de Oliveira Sant Ana, André Oliveira de Souza Lima, Leonardo Rubi Rörig","doi":"10.1007/s00792-023-01324-0","DOIUrl":"10.1007/s00792-023-01324-0","url":null,"abstract":"Mining activities generate large quantities of wastes that significantly alter the biogeochemistry and ecological structure of entire river basins. Microbial communities that develop in these areas present a variety of survival and adaptation mechanisms. Knowing this diversity at the molecular level is strategic both for understanding adaptive processes and for identifying genomes with potential use in bioremediation and bioprospecting. In this work, prokaryotic and eukaryotic communities were evaluated by meta-taxonomics (16S and 18S amplicons) in sediments and water bodies impacted by acid mine drainage in an important coal mining area in southern Brazil. Five sampling stations were defined on a gradient of impacts (pH 2.7-4.25). Taxon diversity was directly proportional to pH, being greater in sediments than in water. The dominant prokaryotic phyla in the samples were Proteobacteria, Actinobacteria, Acidobacteria, OD1, Nitrospirae, and Euryarchaeota, and among the eukaryotes, algae (Ochrophyta, Chlorophyta, Cryptophyceae), fungi (Basidiomycota, Ascomycota, and Cryptomycota), and protists (Ciliophora, Heterolobosea, Cercozoa). The prokaryotic genera Leptospirillum, Acidithiobacillus, Acidiphilium, Thiomonas, Thermogymnomonas, and Acidobacterium, and the eukaryotic genera Pterocystis and Poteriospumella were associated with more acidic conditions and higher metal concentrations, while the prokaryotic genera Sediminibacterium, Gallionella Geothrix, and Geobacter were more abundant in transitional environments.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 1","pages":"8"},"PeriodicalIF":2.9,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138829133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential cellular localization of DNA gyrase and topoisomerase IB in response to DNA damage in Deinococcus radiodurans 放射球孢子菌中 DNA 回旋酶和拓扑异构酶 IB 在 DNA 损伤时的细胞定位差异

IF 2.9 3区生物学

Extremophiles Pub Date : 2023-12-07 DOI: 10.1007/s00792-023-01323-1

Shruti Mishra, Himani Tewari, Reema Chaudhary, Hari S.Misra, Swathi Kota

{"title":"Differential cellular localization of DNA gyrase and topoisomerase IB in response to DNA damage in Deinococcus radiodurans","authors":"Shruti Mishra, Himani Tewari, Reema Chaudhary, Hari S.Misra, Swathi Kota","doi":"10.1007/s00792-023-01323-1","DOIUrl":"https://doi.org/10.1007/s00792-023-01323-1","url":null,"abstract":"Topoisomerases are crucial enzymes in genome maintenance that modulate the topological changes during DNA metabolism. Deinococcus radiodurans, a Gram-positive bacterium is characterized by its resistance to many abiotic stresses including gamma radiation. Its multipartite genome encodes both type I and type II topoisomerases. Time-lapse studies using fluorescently tagged topoisomerase IB (drTopoIB-RFP) and DNA gyrase (GyrA-RFP) were performed to check the dynamics and localization with respect to DNA repair and cell division under normal and post-irradiation growth conditions. Results suggested that TopoIB and DNA gyrase are mostly found on nucleoid, highly dynamic, and show growth phase-dependent subcellular localization. The drTopoIB-RFP was also present at peripheral and septum regions but does not co-localize with the cell division protein, drFtsZ. On the other hand, DNA gyrase co-localizes with PprA a pleiotropic protein involved in radioresistance, on the nucleoid during the post-irradiation recovery (PIR). The topoIB mutant was found to be sensitive to hydroxyurea treatment, and showed more accumulation of single-stranded DNA during the PIR, compared to the wild type suggesting its role in DNA replication stress. Together, these results suggest differential localization of drTopoIB-RFP and GyrA-RFP in D. radiodurans and their interaction with PprA protein, emphasizing the functional significance and role in radioresistance.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"17 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into the rapid metabolism of Geobacillus sp. LC300: unraveling metabolic requirements and optimal growth conditions. Geobacillus sp. LC300快速代谢的研究:阐明代谢需求和最佳生长条件。

IF 2.9 3区生物学

Extremophiles Pub Date : 2023-12-01 DOI: 10.1007/s00792-023-01319-x

Emil Ljungqvist, Jeanett Daga-Quisbert, Antonius van Maris, Martin Gustavsson

{"title":"Insights into the rapid metabolism of Geobacillus sp. LC300: unraveling metabolic requirements and optimal growth conditions.","authors":"Emil Ljungqvist, Jeanett Daga-Quisbert, Antonius van Maris, Martin Gustavsson","doi":"10.1007/s00792-023-01319-x","DOIUrl":"10.1007/s00792-023-01319-x","url":null,"abstract":"This study investigated the metabolism of Geobacillus sp. LC300, a promising biorefinery host organism with high substrate utilization rates. A new defined medium was designed and tested that allows for exponential growth to elevated cell densities suitable for quantitative physiological studies. Screening of the metabolic requirements of G. sp. LC300 revealed prototrophy for all essential amino acids and most vitamins and only showed auxotrophy for vitamin B12 and biotin. The effect of temperature and pH on growth rate was investigated, adjusting the optimal growth temperature to several degrees lower than previously reported. Lastly, studies on carbon source utilization revealed a capability for fast growth on several common carbon sources, including monosaccharides, oligosaccharides, and polysaccharides, and the highest ever reported growth rate in defined medium on glucose (2.20 h-1) or glycerol (1.95 h-1). These findings provide a foundation for further exploration of G. sp. LC300's physiology and metabolic regulation, and its potential use in bioproduction processes.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 1","pages":"6"},"PeriodicalIF":2.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138458887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mining thermophiles for biotechnologically relevant enzymes: evaluating the potential of European and Caucasian hot springs. 为生物技术相关的酶挖掘嗜热菌:评价欧洲和高加索温泉的潜力。

IF 2.9 3区生物学

Extremophiles Pub Date : 2023-11-22 DOI: 10.1007/s00792-023-01321-3

Christin Burkhardt, Leon Baruth, Neele Meyer-Heydecke, Barbara Klippel, Armine Margaryan, Ani Paloyan, Hovik H Panosyan, Garabed Antranikian

{"title":"Mining thermophiles for biotechnologically relevant enzymes: evaluating the potential of European and Caucasian hot springs.","authors":"Christin Burkhardt, Leon Baruth, Neele Meyer-Heydecke, Barbara Klippel, Armine Margaryan, Ani Paloyan, Hovik H Panosyan, Garabed Antranikian","doi":"10.1007/s00792-023-01321-3","DOIUrl":"10.1007/s00792-023-01321-3","url":null,"abstract":"The development of sustainable and environmentally friendly industrial processes is becoming very crucial and demanding for the rapid implementation of innovative bio-based technologies. Natural extreme environments harbor the potential for discovering and utilizing highly specific and efficient biocatalysts that are adapted to harsh conditions. This review focuses on extremophilic microorganisms and their enzymes (extremozymes) from various hot springs, shallow marine vents, and other geothermal habitats in Europe and the Caucasus region. These hot environments have been partially investigated and analyzed for microbial diversity and enzymology. Hotspots like Iceland, Italy, and the Azores harbor unique microorganisms, including bacteria and archaea. The latest results demonstrate a great potential for the discovery of new microbial species and unique enzymes that can be explored for the development of Circular Bioeconomy.Different screening approaches have been used to discover enzymes that are active at extremes of temperature (up 120 °C), pH (0.1 to 11), high salt concentration (up to 30%) as well as activity in the presence of solvents (up to 99%). The majority of published enzymes were revealed from bacterial or archaeal isolates by traditional activity-based screening techniques. However, the latest developments in molecular biology, bioinformatics, and genomics have revolutionized life science technologies. Post-genomic era has contributed to the discovery of millions of sequences coding for a huge number of biocatalysts. Both strategies, activity- and sequence-based screening approaches, are complementary and contribute to the discovery of unique enzymes that have not been extensively utilized so far.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 1","pages":"5"},"PeriodicalIF":2.9,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10665251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138290695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphonate consumers potentially contributing to methane production in Brazilian soda lakes. 膦酸盐消费者可能会增加巴西苏打湖中的甲烷产量。

IF 2.9 3区生物学

Extremophiles Pub Date : 2023-11-21 DOI: 10.1007/s00792-023-01318-y

C R Carvalho, B C E Souza, W Bieluczyk, Y B Feitosa, E Delbaje, P B Camargo, S M Tsai, Marli F Fiore

{"title":"Phosphonate consumers potentially contributing to methane production in Brazilian soda lakes.","authors":"C R Carvalho, B C E Souza, W Bieluczyk, Y B Feitosa, E Delbaje, P B Camargo, S M Tsai, Marli F Fiore","doi":"10.1007/s00792-023-01318-y","DOIUrl":"10.1007/s00792-023-01318-y","url":null,"abstract":"Oxic methane production (OMP) has been reported to significantly contribute to methane emissions from oxic surface waters. Demethylation of organic compounds, photosynthesis-associated methane production, and (bacterio)chlorophyll reduction activity are some of the investigated mechanisms as potential OMP sources related to photosynthetic organisms. Recently, cyanobacteria have often been correlated with methane accumulation and emission in freshwater, marine, and saline systems. The Brazilian Pantanal is the world's largest wetland system, with approximately 10,000 shallow lakes, most of which are highly alkaline and saline extreme environments. We initiated this study with an overall investigation using genetic markers, from which we explored metagenomic and limnological data from the Pantanal soda for five potential OMP pathways. Our results showed a strong positive correlation between dissolved methane concentrations and bloom events. Metagenomic data and nutrients, mainly orthophosphate, nitrogen, iron, and methane concentrations, suggest that the organic phosphorous demethylation pathway has the most potential to drive OMP in lakes with blooms. A specialized bacterial community was identified, including the Cyanobacteria Raphidiopsis, although the bloom does not contain the genes to carry out this process. These data showed enough evidence to infer the occurrence of an OMP pathway at Pantanal soda lakes, including the microbial sources and their relation to the cyanobacterial blooms.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 1","pages":"4"},"PeriodicalIF":2.9,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138176032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating non-fungal eukaryotic diversity in snow in the Antarctic Peninsula region using DNA metabarcoding. 利用DNA元条形码研究南极半岛地区积雪中非真菌真核生物的多样性。

IF 2.9 3区生物学

Extremophiles Pub Date : 2023-11-14 DOI: 10.1007/s00792-023-01322-2

Paulo E A S Câmara, Graciéle C A de Menezes, Fabyano A C Lopes, Thiago da Silva Paiva, Micheline Carvalho-Silva, Peter Convey, Eduardo T Amorim, Luiz H Rosa

{"title":"Investigating non-fungal eukaryotic diversity in snow in the Antarctic Peninsula region using DNA metabarcoding.","authors":"Paulo E A S Câmara, Graciéle C A de Menezes, Fabyano A C Lopes, Thiago da Silva Paiva, Micheline Carvalho-Silva, Peter Convey, Eduardo T Amorim, Luiz H Rosa","doi":"10.1007/s00792-023-01322-2","DOIUrl":"10.1007/s00792-023-01322-2","url":null,"abstract":"Snow is a unique microhabitat, despite being a harsh environment, multiple life forms have adapted to survive in it. While algae, bacteria and fungi are dominant microorganisms in Antarctic snow, little is known about other organisms that may be present in this habitat. We used metabarcoding to investigate DNA sequence diversity of non-fungal eukaryotes present in snow obtained from six different sites across the Maritime Antarctica. A total of 20 taxa were assigned to obtained sequences, representing five Kingdoms (Chromista, Protozoa, Viridiplantae and Metazoa) and four phyla (Ciliophora, Cercozoa, Chlorophyta and Cnidaria). The highest diversity indices were detected in Trinity Peninsula followed by Robert Island, Arctowski Peninsula, Deception Island, King George Island and Snow Island. The most abundant assignments were to Trebouxiophyceae, followed by Chlamydomonas nivalis and Chlamidomonadales. No taxa were detected at all sites. Three potentially new records for Antarctica were detected: two Ciliophora (Aspidisca magna and Stokesia sp.) and the green algae Trebouxia potteri. Our data suggested that similarities found between the sites may be more related with snow physicochemical properties rather than geographic proximity or latitude. This study provides new insights into the diversity and distribution of eukaryotic organisms in Antarctic snow.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 1","pages":"3"},"PeriodicalIF":2.9,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92153422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lignocellulolytic extremozymes and their biotechnological applications. 木质纤维素分解极端酶及其生物技术应用。

IF 2.9 3区生物学

Extremophiles Pub Date : 2023-11-11 DOI: 10.1007/s00792-023-01314-2

Nikita Sharma, Aditi Agarwal, Ananya Bijoy, Sunidhi Pandit, Rakesh Kumar Sharma

{"title":"Lignocellulolytic extremozymes and their biotechnological applications.","authors":"Nikita Sharma, Aditi Agarwal, Ananya Bijoy, Sunidhi Pandit, Rakesh Kumar Sharma","doi":"10.1007/s00792-023-01314-2","DOIUrl":"10.1007/s00792-023-01314-2","url":null,"abstract":"Lignocellulolytic enzymes are used in different industrial and environmental processes. The rigorous operating circumstances of these industries, however, might prevent these enzymes from performing as intended. On the other side, extremozymes are enzymes produced by extremophiles that can function in extremely acidic or basic; hot or cold; under high or low salinity conditions. These severe conditions might denature the normal enzymes that are produced by mesophilic microorganisms. The increased stability of these enzymes has been contributed to a number of conformational modifications in their structures. These modifications may result from a few amino acid substitutions, an improved hydrophobic core, the existence of extra ion pairs and salt bridges, an increase in compactness, or an increase in positively charged amino acids. These enzymes are the best option for industrial and bioremediation activities that must be carried out under difficult conditions due to their improved stability. The review, therefore, discusses lignocellulolytic extremozymes, their structure and mechanisms along with industrial and biotechnological applications.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 1","pages":"2"},"PeriodicalIF":2.9,"publicationDate":"2023-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89717600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Halorubrum hochsteinianum sp. nov., an ancient haloarchaeon from a natural experiment. Halorbrum hochsteinianum sp.nov.，一种来自自然实验的古代卤古生物。

IF 2.9 3区生物学

Extremophiles Pub Date : 2023-10-31 DOI: 10.1007/s00792-023-01320-4

Russell H Vreeland, Ya-Ping Sun, Bei-Bei Wang, Jing Hou, Heng-Lin Cui

{"title":"Halorubrum hochsteinianum sp. nov., an ancient haloarchaeon from a natural experiment.","authors":"Russell H Vreeland, Ya-Ping Sun, Bei-Bei Wang, Jing Hou, Heng-Lin Cui","doi":"10.1007/s00792-023-01320-4","DOIUrl":"10.1007/s00792-023-01320-4","url":null,"abstract":"A single extremely halophilic strain was isolated from salt brine produced when a fresh water lake flooded a large salt mine located beneath the lake. The water that entered this mine contained less than 0.34 M NaCl, but over time, this sealed brine became saturated by Cenozoic age salt (121-125 million-year BCE). The isolated strain requires at least 1.7 M NaCl for survival and grows optimally in 3.1 M NaCl. Therefore, it could not have survived or been present in the waters that flooded this salt mine. The strain grows at a pH range from 6.5 to 9.0 and has a wide tolerance to temperatures from 25 ℃ to at least 60 ℃. The comparison of 16S rRNA and rpoB' genes revealed that strain 1-13-28T is related to Halorubrum tebenquichense DSM 14210T showing 98.6% and 98.1% similarities, respectively. Phylogenetic analyses based on 16S rRNA, rpoB' genes and 122 concatenated archaeal genes show that the strain 1-13-28T consistently forms a cluster with Halorubrum tebenquichense of the genus Halorubrum. Strain 1-13-28T contained sulfated mannosyl glucosyl diether, and the polar lipid profile was identical to those of most Halorubrum species. Based on the overall combination of physiological, phylogenetic, polar lipids and phylogenomic characteristics, strain 1-13-28T (= ATCC 700083T = CGMCC 1.62627T) represents a newly identified species within the genus Halorubrum for which the name Halorubrum hochsteinianum is proposed.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 1","pages":"1"},"PeriodicalIF":2.9,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71422181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of poly (3-hydroxybutyrate) and extracellular polymeric substances from glycerol by the acidophile Acidiphilium cryptum. 嗜酸性隐窝嗜酸性菌从甘油中生产聚（3-羟基丁酸酯）和胞外聚合物。

IF 2.9 3区生物学

Extremophiles Pub Date : 2023-10-17 DOI: 10.1007/s00792-023-01313-3

Ernesto González, Camila Zuleta, Guiselle Zamora, Nataly Maturana, Belén Ponce, María Virginia Rivero, Alberto Rodríguez, Juan Pablo Soto, Felipe Scott, Álvaro Díaz-Barrera

{"title":"Production of poly (3-hydroxybutyrate) and extracellular polymeric substances from glycerol by the acidophile Acidiphilium cryptum.","authors":"Ernesto González, Camila Zuleta, Guiselle Zamora, Nataly Maturana, Belén Ponce, María Virginia Rivero, Alberto Rodríguez, Juan Pablo Soto, Felipe Scott, Álvaro Díaz-Barrera","doi":"10.1007/s00792-023-01313-3","DOIUrl":"10.1007/s00792-023-01313-3","url":null,"abstract":"Acidiphilium cryptum is an acidophilic, heterotrophic, and metallotolerant bacteria able to use dissolved oxygen or Fe(III) as an electron sink. The ability of this extremophile to accumulate poly(3-hydroxybutyrate) (PHB) and secrete extracellular polymeric substances (EPS) has also been reported. Hence, the aim of this work is to characterize the production of PHB and EPS by the wild strain DSM2389 using glycerol in shaken flasks and bioreactor. Results showed that maximum PHB accumulation (37-42% w/w) was obtained using glycerol concentrations of 9 and 15 g L-1, where maximum dry cell weight titers reached 3.6 and 3.9 g L-1, respectively. The culture in the bioreactor showed that PHB accumulation takes place under oxygen limitation, while the redox potential of the culture medium could be used for online monitoring of the PHB production. Recovered EPS was analyzed by Fourier-transform infrared spectroscopy and subjected to gas chromatography-mass spectrometry after cleavage and derivatization steps. These analyses showed the presence of sugars which were identified as mannose, rhamnose and glucose, in a proportion near to 3.2:2.3:1, respectively. Since glycerol had not been used in previous works, these findings suggest the potential of A. cryptum to produce biopolymers from this compound at a large scale with a low risk of microbial contamination due to the low pH of the fermentation process.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"27 3","pages":"30"},"PeriodicalIF":2.9,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41233754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0