Extremophiles最新文献_第5页

Genome-based classification of the family Haloferacaceae and description of five novel species of Halobaculum. 基于基因组的 Haloferacaceae 科分类和 Halobaculum 的五个新物种描述。

IF 2.6 3区生物学

Extremophiles Pub Date : 2024-03-28 DOI: 10.1007/s00792-024-01337-3

Shun Tan, Ling-Rui Zhu, Qing-Ke Zhang, Xin-Yue Dong, Jing Hou, Heng-Lin Cui

{"title":"Genome-based classification of the family Haloferacaceae and description of five novel species of Halobaculum.","authors":"Shun Tan, Ling-Rui Zhu, Qing-Ke Zhang, Xin-Yue Dong, Jing Hou, Heng-Lin Cui","doi":"10.1007/s00792-024-01337-3","DOIUrl":"10.1007/s00792-024-01337-3","url":null,"abstract":"The taxonomic status of some species of Halobellus, Haloferax, Halogranum, and Haloplanus within the family Haloferacaceae was elucidated by phylogenetic, phylogenomic, and comparative genomic analyses. The relative species of each genus should constitute a single species based on the overall genome-related indexes proposed for species demarcation. The cutoff values of AAI (72.1%), ANI (82.2%), and rpoB' gene similarity (90.7%) were proposed to differentiate genera within the family Haloferacaceae. According to these standards, a novel genus related to the genus Halobaculum was proposed to accommodate Halobaculum halophilum Gai3-2 T and Halobaculum salinum NJ-3-1 T. Five halophilic archaeal strains, DT31T, DT55T, DT92T, SYNS20T, and YSMS11T, isolated from a tidal flat and a marine solar saltern in China, were subjected to polyphasic classification. The phenotypic, phylogenetic, phylogenomic, and comparative genomic analyses revealed that strains DT31T (= CGMCC 1.18923 T = JCM 35417 T), DT55T (= CGMCC 1.19048 T = JCM 36147 T), DT92T (= CGMCC 1.19057 T = JCM 36148 T), SYNS20T (= CGMCC 1.62628 T = JCM 36154 T), and YSMS11T (= CGMCC 1.18927 T = JCM 34912 T) represent five novel species of the genus Halobaculum, for which the names, Halobaculum lipolyticum sp. nov., Halobaculum marinum sp. nov., Halobaculum litoreum sp. nov., Halobaculum halobium sp. nov., and Halobaculum limi sp. nov., are proposed.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 2","pages":"22"},"PeriodicalIF":2.6,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140318133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antarctic marine sediment as a source of filamentous fungi-derived antimicrobial and antitumor compounds of pharmaceutical interest. 南极海洋沉积物是丝状真菌产生的具有药用价值的抗菌和抗肿瘤化合物的来源。

IF 2.6 3区生物学

Extremophiles Pub Date : 2024-03-27 DOI: 10.1007/s00792-024-01339-1

Karine Fernandes Camacho, Layssa de Melo Carlos, Suzan Prado Fernandes Bernal, Valéria M de Oliveira, Jorge Luiz Maria Ruiz, Júlia Ronzella Ottoni, Rosemary Vieira, Arthur Neto, Luiz Henrique Rosa, Michel Rodrigo Zambrano Passarini

{"title":"Antarctic marine sediment as a source of filamentous fungi-derived antimicrobial and antitumor compounds of pharmaceutical interest.","authors":"Karine Fernandes Camacho, Layssa de Melo Carlos, Suzan Prado Fernandes Bernal, Valéria M de Oliveira, Jorge Luiz Maria Ruiz, Júlia Ronzella Ottoni, Rosemary Vieira, Arthur Neto, Luiz Henrique Rosa, Michel Rodrigo Zambrano Passarini","doi":"10.1007/s00792-024-01339-1","DOIUrl":"10.1007/s00792-024-01339-1","url":null,"abstract":"Antarctica harbors a microbial diversity still poorly explored and of inestimable biotechnological value. Cold-adapted microorganisms can produce a diverse range of metabolites stable at low temperatures, making these compounds industrially interesting for biotechnological use. The present work investigated the biotechnological potential for antimicrobial and antitumor activity of filamentous fungi and bacteria isolated from marine sediment samples collected at Deception Island, Antarctica. A total of 89 microbial isolates were recovered from marine sediments and submitted to an initial screening for L-glutaminase with antitumoral activity and for antimicrobial metabolites. The isolates Pseudogymnoascus sp. FDG01, Pseudogymnoascus sp. FDG02, and Penicillium sp. FAD33 showed potential antiproliferative action against human pancreatic carcinoma cells while showing no toxic effect on non-tumor cells. The microbial extracts from unidentified three bacteria and four filamentous fungi showed antibacterial activity against at least one tested pathogenic bacterial strain. The isolate FDG01 inhibited four bacterial species, while the isolate FDG01 was active against Micrococcus luteus in the minimal inhibitory concentration of 0.015625 μg mL -1. The results pave the way for further optimization of enzyme production and characterization of enzymes and metabolites found and reaffirm Antarctic marine environments as a wealthy source of compounds potentially applicable in the healthcare and pharmaceutical industry.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 2","pages":"21"},"PeriodicalIF":2.6,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140293235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and enzymatic, biosurfactant and phytotoxic activities of culturable Ascomycota fungi present in marine sediments obtained near the South Shetland Islands, maritime Antarctica. 南极洲南设得兰群岛附近海洋沉积物中可培养 Ascomycota 真菌的多样性及酶活性、生物表面活性剂和植物毒性。

IF 2.6 3区生物学

Extremophiles Pub Date : 2024-03-17 DOI: 10.1007/s00792-024-01336-4

Mayanne Karla da Silva, Débora Luiza Costa Barreto, Rosemary Vieira, Arthur Ayres Neto, Fábio Soares de Oliveira, Peter Convey, Carlos Augusto Rosa, Alysson Wagner Fernandes Duarte, Luiz Henrique Rosa

{"title":"Diversity and enzymatic, biosurfactant and phytotoxic activities of culturable Ascomycota fungi present in marine sediments obtained near the South Shetland Islands, maritime Antarctica.","authors":"Mayanne Karla da Silva, Débora Luiza Costa Barreto, Rosemary Vieira, Arthur Ayres Neto, Fábio Soares de Oliveira, Peter Convey, Carlos Augusto Rosa, Alysson Wagner Fernandes Duarte, Luiz Henrique Rosa","doi":"10.1007/s00792-024-01336-4","DOIUrl":"10.1007/s00792-024-01336-4","url":null,"abstract":"We studied the culturable fungal community recovered from deep marine sediments in the maritime Antarctic, and assessed their capabilities to produce exoenzymes, emulsifiers and metabolites with phytotoxic activity. Sixty-eight Ascomycota fungal isolates were recovered and identified. The most abundant taxon recovered was the yeast Meyerozyma guilliermondii, followed by the filamentous fungi Penicillium chrysogenum, P. cf. palitans, Pseudeurotium cf. bakeri, Thelebolus balaustiformis, Antarctomyces psychrotrophicus and Cladosporium sp. Diversity indices displayed low values overall, with the highest values obtained at shallow depth, decreasing to the deepest location sampled. Only M. guilliermondii and P. cf. palitans were detected in the sediments at all depths sampled, and were the most abundant taxa at all sample sites. The most abundant enzymes detected were proteases, followed by invertases, cellulases, lipases, carrageenases, agarases, pectinases and esterases. Four isolates showed good biosurfactant activity, particularly the endemic species A. psychrotrophicus. Twenty-four isolates of P. cf. palitans displayed strong phytotoxic activities against the models Lactuca sativa and Allium schoenoprasum. The cultivable fungi recovered demonstrated good biosynthetic activity in the production of hydrolytic exoenzymes, biosurfactant molecules and metabolites with phytotoxic activity, reinforcing the importance of documenting the taxonomic, ecological and biotechnological properties of fungi present in deep oceanic sediments of the Southern Ocean.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 2","pages":"20"},"PeriodicalIF":2.6,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140140101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Astrobiological implications of the organic and inorganic cyanide utilization by a novel Antarctic hyperthermophilic Pyrococcus strain. 新型南极嗜热火球菌株利用有机和无机氰化物的天体生物学意义。

IF 2.6 3区生物学

Extremophiles Pub Date : 2024-03-01 DOI: 10.1007/s00792-024-01335-5

Patricio A Uribe-Redlich, Maximiliano J Amenabar, Geraldine V Dennett, Jenny M Blamey

{"title":"Astrobiological implications of the organic and inorganic cyanide utilization by a novel Antarctic hyperthermophilic Pyrococcus strain.","authors":"Patricio A Uribe-Redlich, Maximiliano J Amenabar, Geraldine V Dennett, Jenny M Blamey","doi":"10.1007/s00792-024-01335-5","DOIUrl":"10.1007/s00792-024-01335-5","url":null,"abstract":"Organic and inorganic cyanides are widely distributed in nature, yet not much is known about the ability of microorganisms to use these compounds as a source of nitrogen and/or carbon at high temperatures (>80 °C). Here we studied the capacity of organic and inorganic cyanides to support growth of an hyperthermophilic Pyrococcus strain isolated from Deception Island, Antarctica. This microorganism was capable of growing with aromatic nitriles, aliphatic nitriles, heterocyclic nitriles, amino aromatic nitriles and inorganic cyanides as nitrogen and/or carbon source. This is the first report of an hyperthermophilic microorganism able to incorporate these compounds in its nitrogen and carbon metabolism. Based on enzymatic activity and genomic information, it is possibly that cells of this Pyrococcus strain growing with nitriles or cyanide, might use the carboxylic acid and/or the ammonia generated through the nitrilase enzymatic activity, as a carbon and/or nitrogen source respectively. This work expands the temperature range at which microorganisms can use organic and inorganic cyanides to growth, having important implications to understand microbial metabolisms that can support life on Earth and the possibility to support life elsewhere.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 2","pages":"19"},"PeriodicalIF":2.6,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139996029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel alkane monooxygenase evolved from a broken piece of ribonucleotide reductase in Geobacillus kaustophilus HTA426 isolated from Mariana Trench. 从马里亚纳海沟分离的高嗜地气芽孢杆菌（Geobacillus kaustophilus HTA426）中的核糖核苷酸还原酶断片演化出一种新型烷烃单氧化酶。

IF 2.9 3区生物学

Extremophiles Pub Date : 2024-02-14 DOI: 10.1007/s00792-024-01332-8

Tanasap Nithimethachoke, Chanita Boonmak, Masaaki Morikawa

引用次数: 0

Physiological characterization of polyextremotolerant yeasts from cold environments of Patagonia. 巴塔哥尼亚寒冷环境中多耐寒酵母菌的生理特征。

IF 2.9 3区生物学

Extremophiles Pub Date : 2024-02-11 DOI: 10.1007/s00792-024-01334-6

Rubí A Duo Saito, Martín Moliné, Virginia de Garcia

{"title":"Physiological characterization of polyextremotolerant yeasts from cold environments of Patagonia.","authors":"Rubí A Duo Saito, Martín Moliné, Virginia de Garcia","doi":"10.1007/s00792-024-01334-6","DOIUrl":"10.1007/s00792-024-01334-6","url":null,"abstract":"Yeasts from cold environments have a wide range of strategies to prevent the negative effects of extreme conditions, including the production of metabolites of biotechnological interest. We investigated the growth profile and production of metabolites in yeast species isolated from cold environments. Thirty-eight strains were tested for their ability to grow at different temperatures (5-30 °C) and solute concentrations (3-12.5% NaCl and 50% glucose). All strains tested were able to grow at 5 °C, and 77% were able to grow with 5% NaCl at 18 °C. We were able to group strains based on different physicochemical/lifestyle profiles such as polyextremotolerant, osmotolerant, psychrotolerant, or psychrophilic. Five strains were selected to study biomass and metabolite production (glycerol, trehalose, ergosterol, and mycosporines). These analyses revealed that the accumulation pattern of trehalose and ergosterol was related to each lifestyle profile. Also, our findings would suggest that mycosporines does not have a role as an osmolyte. Non-conventional fermentative yeasts such as Phaffia tasmanica and Saccharomyces eubayanus may be of interest for trehalose production. This work contributes to the knowledge of non-conventional yeasts with biotechnological application from cold environments, including their growth profile, metabolites, and biomass production under different conditions.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 1","pages":"17"},"PeriodicalIF":2.9,"publicationDate":"2024-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139717475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Memoriam: Mosè Rossi (1938–2023) 悼念：摩西-罗西（1938-2023）

IF 2.9 3区生物学

Extremophiles Pub Date : 2024-02-03 DOI: 10.1007/s00792-023-01331-1

Marco Moracci, Beatrice Cobucci-Ponzano, Giuseppe Perugino, Garabed Antranikian

引用次数: 0

Biochemical and in silico structural characterization of a cold-active arginase from the psychrophilic yeast, Glaciozyma antarctica PI12. 精神嗜热酵母 Glaciozyma antarctica PI12 冷活性精氨酸酶的生物化学和硅学结构特征。

IF 2.9 3区生物学

Extremophiles Pub Date : 2024-02-01 DOI: 10.1007/s00792-024-01333-7

Nik Yusnoraini Yusof, Doris Huai Xia Quay, Shazilah Kamaruddin, Mohd Anuar Jonet, Rosli Md Illias, Nor Muhammad Mahadi, Mohd Firdaus-Raih, Farah Diba Abu Bakar, Abdul Munir Abdul Murad

{"title":"Biochemical and in silico structural characterization of a cold-active arginase from the psychrophilic yeast, Glaciozyma antarctica PI12.","authors":"Nik Yusnoraini Yusof, Doris Huai Xia Quay, Shazilah Kamaruddin, Mohd Anuar Jonet, Rosli Md Illias, Nor Muhammad Mahadi, Mohd Firdaus-Raih, Farah Diba Abu Bakar, Abdul Munir Abdul Murad","doi":"10.1007/s00792-024-01333-7","DOIUrl":"10.1007/s00792-024-01333-7","url":null,"abstract":"Glaciozyma antarctica PI12 is a psychrophilic yeast isolated from Antarctica. In this work, we describe the heterologous production, biochemical properties and in silico structure analysis of an arginase from this yeast (GaArg). GaArg is a metalloenzyme that catalyses the hydrolysis of L-arginine to L-ornithine and urea. The cDNA of GaArg was reversed transcribed, cloned, expressed and purified as a recombinant protein in Escherichia coli. The purified protein was active against L-arginine as its substrate in a reaction at 20 °C, pH 9. At 10-35 °C and pH 7-9, the catalytic activity of the protein was still present around 50%. Mn2+, Ni2+, Co2+ and K+ were able to enhance the enzyme activity more than two-fold, while GaArg is most sensitive to SDS, EDTA and DTT. The predicted structure model of GaArg showed a very similar overall fold with other known arginases. GaArg possesses predominantly smaller and uncharged amino acids, fewer salt bridges, hydrogen bonds and hydrophobic interactions compared to the other counterparts. GaArg is the first reported arginase that is cold-active, facilitated by unique structural characteristics for its adaptation of catalytic functions at low-temperature environments. The structure and function of cold-active GaArg provide insights into the potentiality of new applications in various biotechnology and pharmaceutical industries.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"28 1","pages":"15"},"PeriodicalIF":2.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139650645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the multiplicity of geranylgeranyl reductases in Archaea: potential roles in saturation of terpenoids. 揭示古细菌中香叶醇还原酶的多样性：在萜类饱和中的潜在作用。

IF 2.9 3区生物学

Extremophiles Pub Date : 2024-01-27 DOI: 10.1007/s00792-023-01330-2

Alka Rao, Arnold J M Driessen

引用次数: 0

Correction: Limits to the three domains of life: lessons from community assembly along an Antarctic salinity gradient. 更正：生命三大领域的极限：南极盐度梯度群落集结的启示。

IF 2.9 3区生物学

Extremophiles Pub Date : 2024-01-22 DOI: 10.1007/s00792-023-01325-z

Xiaoben Jiang, David J Van Horn, Jordan G Okie, Heather N Buelow, Egbert Schwartz, Daniel R Colman, Kelli L Feeser, Cristina D Takacs-Vesbach

引用次数: 0