Extremophiles最新文献

Proteome-wide mapping of cysteine persulfidation in deep-sea hyperthermophilic archaea. 深海嗜热古细菌中半胱氨酸过硫化的蛋白质组图谱。

IF 2.6 3区生物学

Extremophiles Pub Date : 2026-05-03 DOI: 10.1007/s00792-026-01428-3

Ling Fu, Xuegong Li, Shaowei Liu, Yunhan Zhang, Jing Yang

{"title":"Proteome-wide mapping of cysteine persulfidation in deep-sea hyperthermophilic archaea.","authors":"Ling Fu, Xuegong Li, Shaowei Liu, Yunhan Zhang, Jing Yang","doi":"10.1007/s00792-026-01428-3","DOIUrl":"https://doi.org/10.1007/s00792-026-01428-3","url":null,"abstract":"Protein persulfidation, a major post-translational modification mediated by the gaseous signaling molecule hydrogen sulfide (H₂S), regulates diverse physiological processes in eukaryotes and bacteria. However, its existence and functional roles in archaea, the third domain of life, remain entirely unexplored. Here, we investigate persulfidation in the deep-sea hyperthermophilic archaeon Thermococcus aciditolerans SY113, which thrives in sulfur rich hydrothermal vents and endogenously produces substantial H₂S. Our profiling not only delineated the unique reactivity landscape of this modification but also enabled quantitative analysis of its dynamic regulation by H₂S. A total of 204 persulfidation sites on 171 proteins were identified, of which over 65% were dynamically regulated by H₂S. Further functional analysis suggests that persulfidation represents an ancient and conserved regulatory mechanism in primordial life, including the regulation of catalytic activity and maintenance of protein conformation. Collectively, our findings provide a valuable dataset and a theoretical foundation for understanding the role of persulfidation in the physiological regulation of deep-sea hyperthermophilic archaea.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"30 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147812849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic and functional profiles of two Antarctic chitin-degrading Arthrobacter strains. 两种南极降解几丁质节杆菌菌株的基因组和功能分析。

IF 2.6 3区生物学

Extremophiles Pub Date : 2026-04-23 DOI: 10.1007/s00792-026-01424-7

Yesenia M Santa-Cruz Vasquez, Luis Gabriel Cueva-Yesquen, Luiz Henrique Rosa, Alysson Wagner Fernandes Duarte, Valéria Maia de Oliveira

引用次数: 0

Cultivation and characterization of haloarchaea from Moroccan Atlantic solar salterns: insights into diversity and bioactive potential. 摩洛哥大西洋太阳盐沼盐古菌的培养和特性：对多样性和生物活性潜力的见解。

IF 2.6 3区生物学

Extremophiles Pub Date : 2026-04-17 DOI: 10.1007/s00792-026-01425-6

Imane Charroud, Naima Boum'handi, Mohamed Alouani, Altaf El Blidi, Yannick Fleury, Mohamed Jebbar

引用次数: 0

Fe-driven ROS mitigation in Leptolyngbya JSC-1: optimizing growth using response surface method. 铁驱动的lepolylybya JSC-1活性氧抑制：利用响应面法优化生长

IF 2.6 3区生物学

Extremophiles Pub Date : 2026-04-17 DOI: 10.1007/s00792-026-01427-4

Sikandar Khan, Maryam Akhoundian, Pengcheng Fu

{"title":"Fe-driven ROS mitigation in Leptolyngbya JSC-1: optimizing growth using response surface method.","authors":"Sikandar Khan, Maryam Akhoundian, Pengcheng Fu","doi":"10.1007/s00792-026-01427-4","DOIUrl":"https://doi.org/10.1007/s00792-026-01427-4","url":null,"abstract":"Leptolyngbya JSC-1 is a thermophilic and siderophilic cyanobacterium inhabiting iron-rich hot springs. Response surface method (RSM) is being reported for the first time for optimizing growth conditions of this thermophilic and siderophilic cyanobacterium. Using response surface quadratic model of Box-Behnken design, optimal culture conditions (A: temperature, 45 °C; B: Fe concentration, 42 µM; and C: light intensity, 2000 lx which is equivalent to 27 µmol photons m⁻² s⁻¹ intensity of cool white fluorescent lamp) were determined. The significant model terms were found to be B, AB, A2, B2, and C2. The model R2 value (coefficient of determination) was 0.939, suggesting that the fitted model could explain 93.9% of the total variation. Both the predicted response (OD730 = 2.133) and experimental response (OD730 = 2.1) were in proximity, suggested the appropriateness of the model and RSM. Moreover, an unusual inverse proportion was observed between the Fe concentration and ROS generation with the least ROS generation in JSC-1 grown with 42 µM Fe concentration. Hence, RSM allows evaluating the effects of multiple factors and their interactions on one or more response variables and is recommended to be used for multifactorial optimization studies.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"30 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147716486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extreme Arabian environments and their microbiomes: new frontiers for astrobiology and biosignature discovery. 极端阿拉伯环境及其微生物组：天体生物学和生物特征发现的新前沿。

IF 2.6 3区生物学

Extremophiles Pub Date : 2026-04-09 DOI: 10.1007/s00792-026-01426-5

Júnia Schultz, Paula M García-Martínez, Sharifah Altalhi, Nicholas Kontis, Alef Dos Santos, Alexandre Soares Rosado

引用次数: 0

Optimization of cellulase production using Bacillus thuringiensis strain TS-04-A halophilic bacterium. 苏云金芽孢杆菌TS-04-A嗜盐菌生产纤维素酶的优化。

IF 2.6 3区生物学

Extremophiles Pub Date : 2026-03-16 DOI: 10.1007/s00792-026-01423-8

Vaishnavi Balaji, Aparna Ganapathy Vilasam Sreekala, Vinod Kumar Nathan

引用次数: 0

Common capacity for far-red light photosynthesis in a canyon thermophilic freshwater system. 峡谷嗜热淡水系统中远红光光合作用的一般能力。

IF 2.6 3区生物学

Extremophiles Pub Date : 2026-02-21 DOI: 10.1007/s00792-026-01422-9

Ivan J Moreno, Alexander Bogdanov, Brian Palenik

引用次数: 0

Integration of ploidy, Orc1/Cdc6 homolog function, and lysine acetylation with phosphate limitation and UV stress responses of Haloferax volcanii. 火山盐铁倍性、Orc1/Cdc6同源功能和赖氨酸乙酰化与磷酸盐限制和紫外线胁迫响应的整合。

IF 2.6 3区生物学

Extremophiles Pub Date : 2026-02-10 DOI: 10.1007/s00792-026-01420-x

Daniel Gal, Colton Smith, Kelsey Cooper, Ricardo L Couto-Rodríguez, Julie A Maupin-Furlow

{"title":"Integration of ploidy, Orc1/Cdc6 homolog function, and lysine acetylation with phosphate limitation and UV stress responses of Haloferax volcanii.","authors":"Daniel Gal, Colton Smith, Kelsey Cooper, Ricardo L Couto-Rodríguez, Julie A Maupin-Furlow","doi":"10.1007/s00792-026-01420-x","DOIUrl":"10.1007/s00792-026-01420-x","url":null,"abstract":"Haloarchaea thrive in extreme environments where hypersalinity, DNA damage, and nutrient scarcity pose significant stresses. Here, we provide insight into how defined phosphate levels, UV stress, and Orc1/Cdc6 homolog type influence growth and genome dynamics (ploidy) in the model haloarchaeon Haloferax volcanii. During phosphate limitation, cells exhibited reduced growth, heightened UV sensitivity and dynamic changes in chromosome ploidy, with an early log-phase increase followed by a reduction at later growth stages. To uncover underlying mechanisms, we examined the sixteen Orc1/Cdc6 homologs of H. volcanii using bioinformatics, AI-based structural predictions, genetics, site-directed mutagenesis, and biochemical assays. When constitutively expressed, Orc10 and Orc14 were found to increase ploidy, while Orc1 and Orc10 impaired growth in a manner exacerbated by low phosphate and UV stress. Substitutions at the lysine acetylation site within the Walker A motif (Orc1 K165) and conserved DNA-binding residues (R498 in Orc1 and R304 in Orc14) were found to affect Orc/Cdc6 homolog functions. Moreover, a ∆pat2 lysine acetyltransferase mutant was found to exhibit reduced ploidy and a striking phosphate-dependent flocking phenotype during transition to stationary phase. These results highlight the interplay between phosphate availability, chromosomal regulation, and stress adaptation. Together, this study provides insight into mechanisms that promote H. volcanii survival in conditions that induce DNA damage and phosphate limitation.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"30 1","pages":"10"},"PeriodicalIF":2.6,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12890983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146156720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic insights into abiotic stress tolerance in polar lichen-associated Sphingomonas sp. PAMC26617 and Sphingomonas sp. PAMC26621. 极地地衣相关Sphingomonas sp. PAMC26617和Sphingomonas sp. PAMC26621非生物胁迫耐受性的基因组分析。

IF 2.6 3区生物学

Extremophiles Pub Date : 2026-02-03 DOI: 10.1007/s00792-026-01419-4

Anamika Khanal, So-Ra Han, Jun Hyuck Lee, Tae-Jin Oh

{"title":"Genomic insights into abiotic stress tolerance in polar lichen-associated Sphingomonas sp. PAMC26617 and Sphingomonas sp. PAMC26621.","authors":"Anamika Khanal, So-Ra Han, Jun Hyuck Lee, Tae-Jin Oh","doi":"10.1007/s00792-026-01419-4","DOIUrl":"https://doi.org/10.1007/s00792-026-01419-4","url":null,"abstract":"In this study, Sphingomonas sp. PAMC26617 and Sphingomonas sp. PAMC26621 isolated from rocks of an Arctic lichen, Umbillicaria sp. and Cetraria sp. respectively, were evaluated for their tolerance to abiotic stress of heavy metal(loid)s (HMMs), salt, and osmotic (drought-like) stress at two different temperatures. HMMs resistance protein (CzcC, CzcD, CopB, CopC, CopD, ArsH, and CorC), and osmoprotectant enzymes such as gamma-glutamyl phosphate reductase (ProA), glutamate 5-kinase (ProB), and pyrroline-5-carboxylate reductase (ProC) were identified in both strains during genome analysis. Heavy metal (HM) resistance protein (CorA) was only identified in Sphingomonas sp. PAMC26621. In addition, both strains showed tolerance to CuSO4·5H2O (0.1 mM at 15 °C and 0.25 mM at 25 °C), CoCl2·6H2O (0.25 mM at 15 °C and 0.1 mM at 25 °C), ZnSO4·7H2O (0.1 mM at 15 °C and 25 °C) and Na2HAsO4·7H2O (10 mM at 15 °C and 25 °C), except for Cd(NO3)2·4H2O. Sphingomonas sp. PAMC26617 showed 0.05 mM Cd(NO3)2·4H2O at 15 °C and 0.01 mM at 25 °C and Sphingomonas sp. PAMC26621 showed 0.01 mM Cd(NO3)2·4H2O at 15 °C and 25 °C, respectively. Furthermore, both strains showed tolerance up to 100 mM NaCl and 100 mM D-mannitol at both temperatures. This study is very informative and helpful in understanding the diversity and adaptability of lichen-associated bacteria in harsh environments.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"30 1","pages":"9"},"PeriodicalIF":2.6,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146112729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of anaerobic thermophilic bacterium Caldisericum insulae sp.nov., the second cultivated representative of the phylum Caldisericota. 厌氧嗜热细菌Caldisericum insule sp.nov的分离与特性研究。Caldisericota门的第二个栽培代表。

IF 2.6 3区生物学

Extremophiles Pub Date : 2026-02-03 DOI: 10.1007/s00792-026-01418-5

Nataliya M Ratnikova, A Y Merkel, A A Novikov, A I Slobodkin

{"title":"Isolation and characterization of anaerobic thermophilic bacterium Caldisericum insulae sp.nov., the second cultivated representative of the phylum Caldisericota.","authors":"Nataliya M Ratnikova, A Y Merkel, A A Novikov, A I Slobodkin","doi":"10.1007/s00792-026-01418-5","DOIUrl":"https://doi.org/10.1007/s00792-026-01418-5","url":null,"abstract":"The bacterial phylum Caldisericota has a wide geographical and environmental distribution, but cultivated representatives of this phylum have so far been limited to a single strain. We have isolated a novel anaerobic, thermophilic, fermentative bacterium, strain AR60T from a thermal spring in Sakhalin Island, Russia. Cells of strain AR60T were long rods and tended to form aggregates. Growth was observed between 50 and 70 °C (optimum at 60 °C) and between pH 6.0 and 7.5 (optimum pH 6.5). The isolate was capable for fermentative growth on yeast extract, tryptone and peptone. Thiosulfate, S0, DMSO stimulated growth. The strain contained iso-C17:0 as the major fatty acid. Genome size of strain AR60T was 1.58 Mb; G + C content of the genomic DNA was 34.47%. Phylogenetic analysis revealed that strain AR60T belongs to the genus Caldisericum of the phylum Caldisericota and has 98.85% identity to C. exile. Based on physiological properties and phylogenetic analyses including an average nucleotide identity values and in silico DNA-DNA hybridization, strain AR60T is considered as a new species of the genus Caldisericum. We propose to name strain AR60T (= CGMCC 1.18100T = VKM B-3836T) Caldisericum insulae sp. nov. It is the second cultivated representative of the phylum Caldisericota.","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"30 1","pages":"7"},"PeriodicalIF":2.6,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146112676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0