Genomic signatures of cold adaptation in the family Colwelliaceae.

IF 2.6 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Anais Gentilhomme, Charles Sweet, Gwenn M M Hennon, R Eric Collins
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Abstract

Psychrophily is a phenotype describing microbial growth at low temperatures; elucidating the biomolecular and genomic adaptations necessary for survival in the cold is important for understanding life in extreme environments on Earth and in outer space. We used comparative genomics and temperature growth experiments of bacteria from the family Colwelliaceae to identify genomic factors correlated with optimal growth temperature (OGT). A phylogenomic analysis of 67 public and 39 newly sequenced strains revealed three main clades of Colwelliaceae. Temperature growth experiments revealed significant differences in mean OGT by clade, wherein strains of Colwelliaceae had similar growth rates at -1 °C but varied in their ability to tolerate 17 °C. Using amino acid compositional indices, a multiple linear regression model was constructed to predict the OGT of these organisms (RMSE 5.2 °C). Investigation of Colwelliaceae functional genes revealed a putative cold-adaptive gene cassette that was present in psychrophilic strains but absent in a closely related strain with a significantly higher OGT. This study also presents genomic evidence suggesting that the clade of Colwelliaceae containing Colwellia hornerae should be investigated as a new genus. These contributions offer key insights into the psychrophily phenotype and its underlying genomic foundation in the family Colwelliaceae.

Abstract Image

高良姜科植物适应寒冷的基因组特征。
心理营养是一种描述微生物在低温下生长的表型;阐明在低温下生存所需的生物分子和基因组适应性对于了解地球和外太空极端环境中的生命非常重要。我们利用比较基因组学和高良姜科细菌的温度生长实验来确定与最适生长温度(OGT)相关的基因组因素。对 67 个公开菌株和 39 个新测序菌株进行的系统进化分析表明,高良姜科细菌有三个主要支系。温度生长实验显示,各支系的平均最适生长温度(OGT)存在显著差异。利用氨基酸组成指数,构建了一个多线性回归模型来预测这些生物的 OGT(RMSE 5.2 °C)。对高韦氏菌功能基因的研究发现了一个假定的冷适应基因盒,该基因盒存在于精神嗜性菌株中,但不存在于OGT显著较高的近缘菌株中。本研究还提供了基因组证据,表明包含角柱香(Colwellia hornerae)的高良姜科(Colwelliaceae)支系应作为一个新属进行研究。这些贡献为研究精神嗜性表型及其在高良姜科中的潜在基因组基础提供了重要见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Extremophiles
Extremophiles 生物-生化与分子生物学
CiteScore
6.80
自引率
6.90%
发文量
28
审稿时长
2 months
期刊介绍: Extremophiles features original research articles, reviews, and method papers on the biology, molecular biology, structure, function, and applications of microbial life at high or low temperature, pressure, acidity, alkalinity, salinity, or desiccation; or in the presence of organic solvents, heavy metals, normally toxic substances, or radiation.
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