Fungal diversity present in snow sampled in summer in the north-west Antarctic Peninsula and the South Shetland Islands, Maritime Antarctica, assessed using metabarcoding

IF 2.6 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Extremophiles Pub Date : 2024-04-04 DOI:10.1007/s00792-024-01338-2

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引用次数: 0

Abstract

We assessed the fungal diversity present in snow sampled during summer in the north-west Antarctic Peninsula and the South Shetland Islands, maritime Antarctica using a metabarcoding approach. A total of 586,693 fungal DNA reads were obtained and assigned to 203 amplicon sequence variants (ASVs). The dominant phylum was Ascomycota, followed by Basidiomycota, Mortierellomycota, Chytridiomycota and Mucoromycota. Penicillium sp., Pseudogymnoascus pannorum, Coniochaeta sp., Aspergillus sp., Antarctomyces sp., Phenoliferia sp., Cryolevonia sp., Camptobasidiaceae sp., Rhodotorula mucilaginosa and Bannozyma yamatoana were assessed as abundant taxa. The snow fungal diversity indices were high but varied across the different locations sampled. Of the fungal ASVs detected, only 28 were present all sampling locations. The 116 fungal genera detected in the snow were dominated by saprotrophic taxa, followed by symbiotrophic and pathotrophic. Our data indicate that, despite the low temperature and oligotrophic conditions, snow can host a richer mycobiome than previously reported through traditional culturing studies. The snow mycobiome includes a complex diversity dominated by cosmopolitan, cold-adapted, psychrophilic and endemic taxa. While saprophytes dominate this community, a range of other functional groups are present.

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利用代谢编码评估南极洲西北部和南设得兰群岛夏季采样的雪中真菌多样性

摘要我们采用代谢编码方法评估了夏季在南极半岛西北部和南设得兰群岛（南极洲海洋性气候区）采样的雪中真菌的多样性。共获得 586,693 个真菌 DNA 读数，并分配给 203 个扩增子序列变体 (ASV)。主要门类是子囊菌门，其次是担子菌门、毛霉菌门、糜烂菌门和粘菌门。据评估，青霉属（Penicillium sp.）、假青霉属（Pseudogymnoascus pannorum）、疣壳菌属（Coniochaeta sp.）、曲霉属（Aspergillus sp.）、南极真菌属（Antarctomyces sp.）、Phenoliferia sp.、Cryolevonia sp.、Camptobasidiaceae sp.、Rhodotorula mucilaginosa 和 Bannozyma yamatoana 为丰富的分类群。雪地真菌多样性指数很高，但在不同的采样地点有所不同。在检测到的真菌 ASV 中，只有 28 种存在于所有采样地点。在雪地中检测到的 116 个真菌属中，主要是溶菌性类群，其次是共生和病原性类群。我们的数据表明，尽管雪地温度较低且处于低营养状态，但雪地中的真菌生物群比以前传统的培养研究报告的更为丰富。雪地真菌生物群包括复杂的多样性，以世界性、适应寒冷、嗜心理和地方性分类群为主。虽然寄生菌在这个群落中占主导地位，但也存在一系列其他功能群。

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来源期刊

Extremophiles 生物-生化与分子生物学

CiteScore

6.80

自引率

6.90%

发文量

审稿时长

2 months

期刊介绍： Extremophiles features original research articles, reviews, and method papers on the biology, molecular biology, structure, function, and applications of microbial life at high or low temperature, pressure, acidity, alkalinity, salinity, or desiccation; or in the presence of organic solvents, heavy metals, normally toxic substances, or radiation.