Bioenergetic characterization of hyperthermophilic archaean Methanocaldococcus sp. FS406-22.

IF 2.6 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Addien C Wray, Autum R Downey, Andrea A Nodal, Katherine K Park, Drew Gorman-Lewis
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Abstract

Hyperthermophilic archaean Methanocaldococcus sp. FS406-22 (hereafter FS406) is a hydrogenotrophic methanogen isolated from a deep-sea hydrothermal vent. To better understand the energetic requirements of hydrogen oxidation under extreme conditions, the thermodynamic characterization of FS406 incubations is necessary and notably underexplored. In this work, we quantified the bioenergetics of FS406 incubations at a range of temperatures (65, 76, and 85 ℃) and hydrogen concentrations (1.1, 1.4, and 2.1 mm). The biomass yields (C-mol of biomass per mol of H2 consumed) ranged from 0.02 to 0.19. Growth rates ranged from 0.4 to 1.5 h-1. Gibbs energies of incubation based on macrochemical equations of cell growth ranged from - 198 kJ/C-mol to - 1840 kJ/C-mol. Enthalpies of incubation determined from calorimetric measurements ranged from - 4150 kJ/C-mol to - 36333 kJ/C-mol. FS406 growth rates were most comparable to hyperthermophilic methanogen Methanocaldococcus jannaschii. Maintenance energy calculations from the thermodynamic parameters of FS406 and previously determined heterotrophic methanogen data revealed that temperature is a primary determinant rather than an electron donor. This work provides new insights into the thermodynamic underpinnings of a hyperthermophilic hydrothermal vent methanogen and helps to better constrain the energetic requirements of life in extreme environments.

Abstract Image

超嗜热古菌 Methanocaldococcus sp. FS406-22 的生物能特征。
嗜热古菌 Methanocaldococcus sp.FS406-22(以下简称 FS406)是从深海热液喷口中分离出来的一种亲氢甲烷菌。为了更好地了解极端条件下氢氧化的能量需求,有必要对 FS406 培养过程进行热力学表征,但这方面的研究明显不足。在这项工作中,我们对一系列温度(65、76 和 85 ℃)和氢浓度(1.1、1.4 和 2.1 毫米)下 FS406 培养的生物能进行了量化。生物量产量(每摩尔氢消耗的 C 摩尔生物量)从 0.02 到 0.19 不等。生长速率从 0.4 到 1.5 h-1 不等。根据细胞生长的宏观化学方程式得出的培养吉布斯能介于 - 198 kJ/C-mol 到 - 1840 kJ/C-mol 之间。通过热量测量确定的培养焓介于 - 4150 kJ/C-mol 到 - 36333 kJ/C-mol 之间。FS406 的生长率与超嗜热甲烷菌 Methanocaldococcus jannaschii 的生长率最为接近。根据 FS406 的热力学参数和先前确定的异养甲烷菌数据进行的维持能计算显示,温度是主要的决定因素,而不是电子供体。这项工作为了解热液喷口超嗜热甲烷菌的热力学基础提供了新的视角,有助于更好地限制极端环境中生命的能量需求。
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来源期刊
Extremophiles
Extremophiles 生物-生化与分子生物学
CiteScore
6.80
自引率
6.90%
发文量
28
审稿时长
2 months
期刊介绍: Extremophiles features original research articles, reviews, and method papers on the biology, molecular biology, structure, function, and applications of microbial life at high or low temperature, pressure, acidity, alkalinity, salinity, or desiccation; or in the presence of organic solvents, heavy metals, normally toxic substances, or radiation.
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