热核硝化地杆菌 HBB 111 菌株产生的新型外多糖的特性和生物活性。

IF 2.6 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Mehmet Aytar, Gamze Başbülbül, Deniz Aktaş Uygun
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引用次数: 0

摘要

本研究优化了嗜热微生物 Geobacillus thermodenitrificans HBB 111 的 EPS 生产条件,发现其 EPS(EPS 111)产量为 44.0 mg/L。利用离子交换色谱法和凝胶过滤色谱法纯化了 EPS 111,得到了单一类型的外多糖。通过 TLC、傅立叶变换红外光谱、核磁共振和气相色谱-质谱对纯化的 EPS 111 的结构进行了评估,结果表明它含有六糖(葡萄糖、果糖、半乳糖和甘露糖)和戊糖。从扫描电镜照片可以看出,EPS 111 具有无定形、粗糙和分层结构。研究发现,纯化的 EPS 111 细胞毒性低(2.3%),具有很高的抗氧化活性和显著的抗糖尿病、益生元和纤维蛋白溶解活性。与文献报道的嗜热 EPS 相比,本研究纯化的 EPS 111 具有多种生物活性,在生物技术和生物医学领域具有很大的应用潜力,这一点非常有价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Characterization and biological activities of a novel exopolysaccharide produced from Geobacillus thermodenitrificans HBB 111 strain.

Characterization and biological activities of a novel exopolysaccharide produced from Geobacillus thermodenitrificans HBB 111 strain.

In this study, EPS production conditions of Geobacillus thermodenitrificans HBB 111, a thermophilic microorganism, were optimized and the amount of produced EPS (EPS 111) was found to be 44.0 mg/L. EPS 111 was purified using ion exchange chromatography and gel filtration chromatography, and a single type of exopolysaccharide was obtained. The structure of the purified EPS 111 was evaluated by TLC, FTIR, NMR, and GC-MS, and it was observed that it contained hexose (glucose, fructose, galactose and mannose) and pentose sugars. From the SEM photographs, it was understood that EPS 111 had an amorphous, rough, and layered structure. It was found that purified EPS 111 had low cytotoxicity (2.3%) and exhibited high antioxidant activity and remarkable antidiabetic, prebiotic and fibrinolytic activities. It is very valuable that the purified EPS 111 in this study offers multiple biological activities compared to the thermophilic EPSs reported in the literature and has a high potential for use in biotechnological and biomedical fields.

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来源期刊
Extremophiles
Extremophiles 生物-生化与分子生物学
CiteScore
6.80
自引率
6.90%
发文量
28
审稿时长
2 months
期刊介绍: Extremophiles features original research articles, reviews, and method papers on the biology, molecular biology, structure, function, and applications of microbial life at high or low temperature, pressure, acidity, alkalinity, salinity, or desiccation; or in the presence of organic solvents, heavy metals, normally toxic substances, or radiation.
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