Untangling the adaptive strategies of thermophilic bacterium Anoxybacillus rupiensis TPH1 under low temperature.

IF 2.6 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Aditi Mishra, Sindhunath Chakraborty, Tameshwar Prasad Jaiswal, Samujjal Bhattacharjee, Shreya Kesarwani, Arun Kumar Mishra, Satya Shila Singh
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Abstract

The present study investigates the low temperature tolerance strategies of thermophilic bacterium Anoxybacillus rupiensis TPH1, which grows optimally at 55 °C , by subjecting it to a temperature down-shift of 10 °C (45 °C) for 4 and 6 h followed by studying its growth, morphophysiological, molecular and proteomic responses. Results suggested that although TPH1 experienced increased growth inhibition, ROS production, protein oxidation and membrane disruption after 4 h of incubation at 45 °C yet maintained its DNA integrity and cellular structure through the increased expression of DNA damage repair and cell envelop synthesizing proteins and also progressively alleviated growth inhibition by 20% within two hours i.e., 6 h, by inducing the expression of antioxidative enzymes, production of unsaturated fatty acids, capsular and released exopolysaccharides and forming biofilm along with chemotaxis proteins. Conclusively, the adaptation of Anoxybacillus rupiensis TPH1 to lower temperature is mainly mediated by the synthesis of large numbers of defense proteins and exopolysaccharide rich biofilm formation.

Abstract Image

解开嗜热细菌 Anoxybacillus rupiensis TPH1 在低温下的适应策略。
本研究调查了嗜热细菌 Anoxybacillus rupiensis TPH1 的低温耐受策略,该细菌的最佳生长温度为 55 °C,研究方法是将其置于 10 °C(45 °C)的低温环境中 4 小时和 6 小时,然后研究其生长、形态生理学、分子和蛋白质组反应。结果表明,虽然 TPH1 在 45 ℃ 温育 4 小时后,生长抑制、ROS 生成、蛋白质氧化和膜破坏的情况增加,但通过增加 DNA 损伤修复和细胞包膜合成蛋白的表达,保持了其 DNA 的完整性和细胞结构,并通过诱导抗氧化酶的表达、不饱和脂肪酸的生成、胶囊和外多糖的释放、生物膜的形成以及趋化蛋白的表达,在两小时内(即 6 小时)将生长抑制逐步缓解了 20%。综上所述,Anoxybacillus rupiensis TPH1 对低温的适应主要是通过合成大量防御蛋白和形成富含外多糖的生物膜来实现的。
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来源期刊
Extremophiles
Extremophiles 生物-生化与分子生物学
CiteScore
6.80
自引率
6.90%
发文量
28
审稿时长
2 months
期刊介绍: Extremophiles features original research articles, reviews, and method papers on the biology, molecular biology, structure, function, and applications of microbial life at high or low temperature, pressure, acidity, alkalinity, salinity, or desiccation; or in the presence of organic solvents, heavy metals, normally toxic substances, or radiation.
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