FEMS yeast research最新文献_第7页

Exploring the antioxidant activity of Fe(III), Mn(III)Mn(II), and Cu(II) compounds in Saccharomyces cerevisiae and Galleria mellonella models of study 探究铁（III）、锰（III）、锰（II）和铜（II）化合物在酿酒酵母和黑僵菌模型中的抗氧化活性

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-12-21 DOI: 10.1093/femsyr/foad052

Larissa M M Mattos, Hyan M Hottum, Daniele C Pires, Bruna B Segat, Adolfo Horn, Christiane Fernandes, Marcos D Pereira

{"title":"Exploring the antioxidant activity of Fe(III), Mn(III)Mn(II), and Cu(II) compounds in Saccharomyces cerevisiae and Galleria mellonella models of study","authors":"Larissa M M Mattos, Hyan M Hottum, Daniele C Pires, Bruna B Segat, Adolfo Horn, Christiane Fernandes, Marcos D Pereira","doi":"10.1093/femsyr/foad052","DOIUrl":"https://doi.org/10.1093/femsyr/foad052","url":null,"abstract":"Reactive oxygen species (ROS) are closely related to oxidative stress, aging, and the onset of human diseases. To mitigate ROS-induced damages, extensive research has focused on examining the antioxidative attributes of various synthetic/natural substances. Coordination compounds serving as synthetic antioxidants have emerged as a promising approach to attenuate ROS toxicity. Herein, we investigated the antioxidant potential of a series of Fe(III) (1), Mn(III)Mn(II) (2) and Cu(II) (3) coordination compounds synthesized with the ligand N-(2-hydroxybenzyl)-N-(2-pyridylmethyl)[(3-chloro)(2-hydroxy)]-propylamine in Saccharomyces cerevisiae exposed to oxidative stress. We also assessed the antioxidant potential of these complexes in the alternative model of study, Galleria mellonella. DPPH analysis indicated that these complexes presented moderate antioxidant activity. However, treating Saccharomyces cerevisiae with 1, 2 and 3 increased the tolerance against oxidative stress and extended yeast lifespan. The treatment of yeast cells with these complexes decreased lipid peroxidation and catalase activity in stressed cells, whilst no change in SOD activity was observed. Moreover, these complexes induced the Hsp104 expression. In G. mellonella, complex administration extended larval survival under H2O2 stress and did not affect the insect's life cycle. Our results suggest that the antioxidant potential exhibited by these complexes could be further explored to mitigate various oxidative stress-related disorders.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":"10 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138824596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

QTL mapping reveals novel genes and mechanisms underlying variations in H2S production during alcoholic fermentation in Saccharomyces cerevisiae QTL 图谱揭示了酿酒酵母在酒精发酵过程中产生 H2S 的新基因和新机制

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-12-16 DOI: 10.1093/femsyr/foad050

Irene De Guidi, Céline Serre, Jessica Noble, Anne Ortiz-Julien, Bruno Blondin, Jean-Luc Legras

{"title":"QTL mapping reveals novel genes and mechanisms underlying variations in H2S production during alcoholic fermentation in Saccharomyces cerevisiae","authors":"Irene De Guidi, Céline Serre, Jessica Noble, Anne Ortiz-Julien, Bruno Blondin, Jean-Luc Legras","doi":"10.1093/femsyr/foad050","DOIUrl":"https://doi.org/10.1093/femsyr/foad050","url":null,"abstract":"Saccharomyces cerevisiae requirement for reduced sulfur to synthesise methionine and cysteine during alcoholic fermentation, is mainly fulfilled through the sulfur assimilation pathway. S. cerevisiae reduces sulfate into sulfur dioxide (SO2) and sulfide (H2S), whose overproduction is a major issue in winemaking, due to its negative impact on wine aroma. The amount of H2S produced is highly strain-specific and also depends on SO2 concentration, often added to grape must. Applying a Bulk Segregant Analysis to a 96 strain-progeny derived from two strains with different abilities to produce H2S, and comparing allelic frequencies along the genome of pools of segregants producing contrasting H2S quantities, we identified two causative regions involved in H2S production in the presence of SO2. A functional genetic analysis allowed the identification of variants in four genes able to impact H2S formation, viz; ZWF1, ZRT2, SNR2 and YLR125W, and involved in functions and pathways not associated with sulfur metabolism until now. These data point out that, in wine fermentation conditions, redox status and zinc homeostasis are linked to H2S formation while providing new insights into the regulation of H2S production, and a new vision of the interplay between the sulfur assimilation pathway and cell metabolism.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":"1 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138824212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

My Journey with Yeast. 我的酵母之旅。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-06-22 DOI: 10.1093/femsyr/foad035

Charles Abbas

引用次数: 0

Travels with Metschnikowia 与Metschnikowia一起旅行

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-04-18 DOI: 10.1093/femsyr/foad025

M. Lachance

引用次数: 0

Metabolic engineering of Saccharomyces cerevisiae for glycerol utilization. 甘油利用的酿酒酵母代谢工程。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad014

Ziqian Yu, Zhao Chang, Yinhua Lu, Han Xiao

引用次数: 0

Industry and academia-a perfect match. 工业界和学术界是完美的一对。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foac061

Hennie J J van Vuuren, Terrance G Cooper

{"title":"Industry and academia-a perfect match.","authors":"Hennie J J van Vuuren, Terrance G Cooper","doi":"10.1093/femsyr/foac061","DOIUrl":"https://doi.org/10.1093/femsyr/foac061","url":null,"abstract":"My career developed very differently from those of most academic researchers. After school, I worked for 6 years in industries that employed yeast to manufacture ethanol and beer. At university, I was trained as a microbiologist with very little training in molecular biology. I retrained in 1987 in molecular yeast genetics and focused on genetic engineering of industrial yeasts to minimize the production of spoilage compounds in wine and ethyl carbamate, a carcinogen, in wine. The malolactic yeast ML01 and the urea-degrading yeast were the first genetically enhanced yeasts that obtained US FDA approval for commercial applications. Apart from applied research, I was fascinated by classic molecular yeast genetic studies using sophisticated techniques such as transcriptomics, proteomics, and metabolomics. Doing research at the University of British Columbia was stimulating and exciting, we established a core microarray and metabolomics facility that was used by many scientists at UBC and hospitals in Vancouver. I also established a state-of-the-art Wine Library that was used to study aging of wines produced in British Columbia. Finally, I have been fortunate to know and collaborate with leading yeast scientists who motivated me.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":"23 ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9089750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

pCEC-red: a new vector for easier and faster CRISPR-Cas9 genome editing in Saccharomyces cerevisiae. pCEC-red:在酿酒酵母中更容易和更快地进行CRISPR-Cas9基因组编辑的新载体

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad002

Letizia Maestroni, Pietro Butti, Vittorio Giorgio Senatore, Paola Branduardi

{"title":"pCEC-red: a new vector for easier and faster CRISPR-Cas9 genome editing in Saccharomyces cerevisiae.","authors":"Letizia Maestroni, Pietro Butti, Vittorio Giorgio Senatore, Paola Branduardi","doi":"10.1093/femsyr/foad002","DOIUrl":"10.1093/femsyr/foad002","url":null,"abstract":"CRISPR-Cas9 technology is widely used for precise and specific editing of Saccharomyces cerevisiae genome to obtain marker-free engineered hosts. Targeted double-strand breaks are controlled by a guide RNA (gRNA), a chimeric RNA containing a structural segment for Cas9 binding and a 20-mer guide sequence that hybridises to the genomic DNA target. Introducing the 20-mer guide sequence into gRNA expression vectors often requires complex, time-consuming, and/or expensive cloning procedures. We present a new plasmid for CRISPR-Cas9 genome editing in S. cerevisiae, pCEC-red. This tool allows to (i) transform yeast with both Cas9 and gRNA expression cassettes in a single plasmid and (ii) insert the 20-mer sequence in the plasmid with high efficiency, thanks to Golden Gate Assembly and (iii) a red chromoprotein-based screening to speed up the selection of correct plasmids. We tested genome-editing efficiency of pCEC-red by targeting the ADE2 gene. We chose three different 20-mer targets and designed two types of repair fragments to test pCEC-red for precision editing and for large DNA region replacement procedures. We obtained high efficiencies (∼90%) for both engineering procedures, suggesting that the pCEC system can be used for fast and reliable marker-free genome editing.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":"23 ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/35/69/foad002.PMC9906608.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9587562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The genome sequence of the Champagne Epernay Geisenheim wine yeast reveals its hybrid nature. 香槟Epernay Geisenheim葡萄酒酵母的基因组序列揭示了其杂交性质。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad033

Beatrice Bernardi, Florian Michling, Judith Muno-Bender, Katrin Matti, Jürgen Wendland

{"title":"The genome sequence of the Champagne Epernay Geisenheim wine yeast reveals its hybrid nature.","authors":"Beatrice Bernardi, Florian Michling, Judith Muno-Bender, Katrin Matti, Jürgen Wendland","doi":"10.1093/femsyr/foad033","DOIUrl":"https://doi.org/10.1093/femsyr/foad033","url":null,"abstract":"Lager yeasts are hybrids between Saccharomyces cerevisiae and S. eubayanus. Wine yeast biodiversity, however, has only recently been discovered to include besides pure S. cerevisiae strains also hybrids between different Saccharomyces yeasts as well as introgressions from non-Saccharomyces species. Here, we analysed the genome of the Champagne Epernay Geisenheim (CEG) wine yeast. This yeast is an allotetraploid (4n - 1) hybrid of S. cerevisiae harbouring a substantially reduced S. kudriavzevii genome contributing only 1/3 of a full genome equivalent. We identified a novel oligopeptide transporter gene, FOT4, in CEG located on chromosome XVI. FOT genes were originally derived from Torulaspora microellipsoides and FOT4 arose by non-allelic recombination between adjacent FOT1 and FOT2 genes. Fermentations of CEG in Riesling and Müller-Thurgau musts were compared with the S. cerevisiae Geisenheim wine yeast GHM, which does not carry FOT genes. At low temperature (10°C), CEG completed fermentations faster and produced increased levels of higher alcohols (e.g. isoamyl alcohol). At higher temperature (18°C), CEG produced higher amounts of the pineapple-like alkyl esters i-butyric and propionic acid ethyl esters compared to GHM. The hybrid nature of CEG thus provides advantages in grape must fermentations over S. cerevisiae wine yeasts, especially with regard to aroma production.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":"23 ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10117892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

How to think about and do successful research What you probable did not learn when you first entered the laboratory. 如何思考和进行成功的研究你刚进实验室时可能没有学到的东西。

IF 2.4 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foac065

Terrance G Cooper

引用次数: 0

Split-marker-mediated genome editing improves homologous recombination frequency in the CTG clade yeast Candida intermedia. 分裂标记介导的基因组编辑提高了CTG分支酵母假丝酵母中间介质的同源重组频率。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad016

Kameshwara V R Peri, Fábio Faria-Oliveira, Adam Larsson, Alexander Plovie, Nicolas Papon, Cecilia Geijer

{"title":"Split-marker-mediated genome editing improves homologous recombination frequency in the CTG clade yeast Candida intermedia.","authors":"Kameshwara V R Peri, Fábio Faria-Oliveira, Adam Larsson, Alexander Plovie, Nicolas Papon, Cecilia Geijer","doi":"10.1093/femsyr/foad016","DOIUrl":"https://doi.org/10.1093/femsyr/foad016","url":null,"abstract":"Genome-editing toolboxes are essential for the exploration and exploitation of nonconventional yeast species as cell factories, as they facilitate both genome studies and metabolic engineering. The nonconventional yeast Candida intermedia is a biotechnologically interesting species due to its capacity to convert a wide range of carbon sources, including xylose and lactose found in forestry and dairy industry waste and side-streams, into added-value products. However, possibilities of genetic manipulation have so far been limited due to lack of molecular tools for this species. We describe here the development of a genome editing method for C. intermedia, based on electroporation and gene deletion cassettes containing the Candida albicans NAT1 dominant selection marker flanked by 1000 base pair sequences homologous to the target loci. Linear deletion cassettes targeting the ADE2 gene originally resulted in <1% targeting efficiencies, suggesting that C. intermedia mainly uses nonhomologous end joining for integration of foreign DNA fragments. By developing a split-marker based deletion technique for C. intermedia, we successfully improved the homologous recombination rates, achieving targeting efficiencies up to 70%. For marker-less deletions, we also employed the split-marker cassette in combination with a recombinase system, which enabled the construction of double deletion mutants via marker recycling. Overall, the split-marker technique proved to be a quick and reliable method for generating gene deletions in C. intermedia, which opens the possibility to uncover and enhance its cell factory potential.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":"23 ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10035504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9653578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1