FEMS yeast research最新文献_第7页

Correction to: Elevated energy costs of biomass production in mitochondrial respiration-deficient Saccharomyces cerevisiae. 更正:线粒体呼吸缺陷的酿酒酵母生物质生产的能源成本升高。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad015

引用次数: 0

Grape-associated fungal community patterns persist from berry to wine on a fine geographical scale. 葡萄相关的真菌群落模式从浆果到葡萄酒在一个良好的地理尺度上持续存在。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foac067

Jonathan T Martiniuk, Jonah Hamilton, Thomas Dodsworth, Vivien Measday

{"title":"Grape-associated fungal community patterns persist from berry to wine on a fine geographical scale.","authors":"Jonathan T Martiniuk, Jonah Hamilton, Thomas Dodsworth, Vivien Measday","doi":"10.1093/femsyr/foac067","DOIUrl":"https://doi.org/10.1093/femsyr/foac067","url":null,"abstract":"Wine grape fungal community composition is influenced by abiotic factors including geography and vintage. Compositional differences may correlate with different wine metabolite composition and sensory profiles, suggesting a microbial role in the shaping of a wine's terroir, or regional character. While grape and wine-associated fungal community composition has been studied extensively at a regional and sub-regional scale, it has not been explored in detail on fine geographical scales over multiple harvests. Over two years, we examined the fungal communities on Vitis Vinifera cv. Pinot noir grape berry surfaces, in crushed grapes, and in lab spontaneous fermentations from three vineyards within a < 1 km radius in Canada's Okanagan Valley wine region. We also evaluated the effect of winery environment exposure on fungal community composition by sampling grapes crushed and fermented in the winery at commercial scale. Spatiotemporal community structure was evident among grape berry surface, crushed grape and fermentation samples, with each vineyard exhibiting a distinct fungal community signature. Crushed grape fungal populations were richer in fermentative yeast species compared to grape berry surface fungal populations. Our study suggests that, as on a regional level, fungal populations may contribute to fine-scale -terroir,' with significant implications for single-vineyard wines.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/69/35/foac067.PMC9876423.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10697578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The prevalence of killer yeasts and double-stranded RNAs in the budding yeast Saccharomyces cerevisiae. 酿酒酵母芽中杀伤性酵母和双链RNA的普遍性。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad046

Angela M Crabtree, Nathan T Taggart, Mark D Lee, Josie M Boyer, Paul A Rowley

{"title":"The prevalence of killer yeasts and double-stranded RNAs in the budding yeast Saccharomyces cerevisiae.","authors":"Angela M Crabtree, Nathan T Taggart, Mark D Lee, Josie M Boyer, Paul A Rowley","doi":"10.1093/femsyr/foad046","DOIUrl":"10.1093/femsyr/foad046","url":null,"abstract":"Killer toxins are antifungal proteins produced by many species of \"killer\" yeasts, including the brewer's and baker's yeast Saccharomyces cerevisiae. Screening 1270 strains of S. cerevisiae for killer toxin production found that 50% are killer yeasts, with a higher prevalence of yeasts isolated from human clinical samples and winemaking processes. Since many killer toxins are encoded by satellite double-stranded RNAs (dsRNAs) associated with mycoviruses, S. cerevisiae strains were also assayed for the presence of dsRNAs. This screen identified that 51% of strains contained dsRNAs from the mycovirus families Totiviridae and Partitiviridae, as well as satellite dsRNAs. Killer toxin production was correlated with the presence of satellite dsRNAs but not mycoviruses. However, in most killer yeasts, whole genome analysis identified the killer toxin gene KHS1 as significantly associated with killer toxin production. Most killer yeasts had unique spectrums of antifungal activities compared to canonical killer toxins, and sequence analysis identified mutations that altered their antifungal activities. The prevalence of mycoviruses and killer toxins in S. cerevisiae is important because of their known impact on yeast fitness, with implications for academic research and industrial application of this yeast species.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10664976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71479905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modern yeast development: finding the balance between tradition and innovation in contemporary winemaking. 现代酵母开发：在当代葡萄酒酿造中寻找传统与创新之间的平衡。

IF 2.4 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foac049

Jennifer M Gardner, Lucien Alperstein, Michelle E Walker, Jin Zhang, Vladimir Jiranek

{"title":"Modern yeast development: finding the balance between tradition and innovation in contemporary winemaking.","authors":"Jennifer M Gardner, Lucien Alperstein, Michelle E Walker, Jin Zhang, Vladimir Jiranek","doi":"10.1093/femsyr/foac049","DOIUrl":"10.1093/femsyr/foac049","url":null,"abstract":"A key driver of quality in wines is the microbial population that undertakes fermentation of grape must. Winemakers can utilise both indigenous and purposefully inoculated yeasts to undertake alcoholic fermentation, imparting wines with aromas, flavours and palate structure and in many cases contributing to complexity and uniqueness. Importantly, having a toolbox of microbes helps winemakers make best use of the grapes they are presented with, and tackle fermentation difficulties with flexibility and efficiency. Each year the number of strains available commercially expands and more recently, includes strains of non-Saccharomyces, strains that have been improved using both classical and modern yeast technology and mixed cultures. Here we review what is available commercially, and what may be in the future, by exploring recent advances in fermentation relevant strain improvement technologies. We also report on the current use of microbes in the Australian wine industry, as reported by winemakers, as well as regulations around, and sentiment about the potential use of genetically modified organisms in the future.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9990983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9485838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering a fermenting yeast able to produce the fragrant β-ionone apocarotenoid for enhanced aroma properties in wine. 设计一种能够产生芳香的β-离子酮类伪胡萝卜素的发酵酵母，以增强葡萄酒的香气特性。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad001

J J B Timmins, N van Wyk, H Kroukamp, R S K Walker, S Fritsch, D Rauhut, C von Wallbrunn, I S Pretorius, I T Paulsen

{"title":"Engineering a fermenting yeast able to produce the fragrant β-ionone apocarotenoid for enhanced aroma properties in wine.","authors":"J J B Timmins, N van Wyk, H Kroukamp, R S K Walker, S Fritsch, D Rauhut, C von Wallbrunn, I S Pretorius, I T Paulsen","doi":"10.1093/femsyr/foad001","DOIUrl":"https://doi.org/10.1093/femsyr/foad001","url":null,"abstract":"Wine is composed of multitudinous flavour components and volatile organic compounds that provide this beverage with its attractive properties of taste and aroma. The perceived quality of a wine can be attributed to the absolute and relative concentrations of favourable aroma compounds; hence, increasing the detectable levels of an attractive aroma, such as β-ionone with its violet and berry notes, can improve the organoleptic qualities of given wine styles. We here describe the generation of a new grape-must fermenting strain of Saccharomyces cerevisiae that is capable of releasing β-ionone through the heterologous expression of both the enzyme carotenoid cleavage dioxygenase 1 (CCD1) and its substrate, β-carotene. Haploid laboratory strains of S. cerevisiae were constructed with and without integrated carotenogenic genes and transformed with a plasmid containing the genes of CCD1. These strains were then mated with a sporulated diploid wine industry yeast, VIN13, and four resultant crosses-designated MQ01-MQ04-which were capable of fermenting the must to dryness were compared for their ability to release β-ionone. Analyses of their fermentation products showed that the MQ01 strain produced a high level of β-ionone and offers a fermenting hybrid yeast with the potential to enhance the organoleptic qualities of wine.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10692068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How do engineered Yarrowia lipolytica strains secrete free fatty acids: hints from comparative transcriptomics. 工程多脂耶氏菌菌株如何分泌游离脂肪酸:来自比较转录组学的提示。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad027

José Manuel Salvador López, Lea Vidal, Michelle Patricia Adiutama, Filip Van Nieuwerburgh, Dieter Deforce, Jean-Marc Nicaud, Inge Noëlle Adrienne Van Bogaert

{"title":"How do engineered Yarrowia lipolytica strains secrete free fatty acids: hints from comparative transcriptomics.","authors":"José Manuel Salvador López, Lea Vidal, Michelle Patricia Adiutama, Filip Van Nieuwerburgh, Dieter Deforce, Jean-Marc Nicaud, Inge Noëlle Adrienne Van Bogaert","doi":"10.1093/femsyr/foad027","DOIUrl":"https://doi.org/10.1093/femsyr/foad027","url":null,"abstract":"Yarrowia lipolytica has been considered one of the most promising platforms for the microbial production of fatty acids and derived products. The deletion of the faa1 gene coding for an acyl-CoA synthetase leads to the accumulation and secretion of free fatty acids (FFAs) into the extracellular space. The secretion of products is beneficial for the development of microbial cell factories to avoid intracellular inhibitory effects and reduce downstream processing costs. However, the mechanism behind the secretion of fatty acids is not well known. As a starting point, we compared the transcriptome of this mutant showing FFA secretion to a wildtype-like strain not showing this phenotype. The 12 most upregulated genes were evaluated for involvement in FFA secretion by the creation of deletion and overexpression mutants, among them MCH2, YMOH, three cell wall proteins CWP3, CWP4, and CWP11, M12B, and three proteins with unknown functions YUP1, YUP2, and YUP3. None of these proteins take a clear or isolated role in FFA export. As the transcriptomic data revealed an overrepresentation of cell wall-related proteins, some of them were further examined on a theoretical and experimental way. Surprisingly, overexpression of Ygpi led to the production of FFAs in the wildtype-like genetic background. Finally, some of the evaluated genes showed involvement in resistance to FFA toxicity.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10033779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Finding a correct species assignment for a Metschnikowia strain: insights from the genome sequencing of strain DBT012. 为Metschnikowia菌株寻找正确的物种分配:来自菌株DBT012基因组测序的见解。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad024

Eleonora Troiano, Ilaria Larini, Renato L Binati, Veronica Gatto, Sandra Torriani, Pietro Buzzini, Benedetta Turchetti, Elisa Salvetti, Giovanna E Felis

{"title":"Finding a correct species assignment for a Metschnikowia strain: insights from the genome sequencing of strain DBT012.","authors":"Eleonora Troiano, Ilaria Larini, Renato L Binati, Veronica Gatto, Sandra Torriani, Pietro Buzzini, Benedetta Turchetti, Elisa Salvetti, Giovanna E Felis","doi":"10.1093/femsyr/foad024","DOIUrl":"https://doi.org/10.1093/femsyr/foad024","url":null,"abstract":"Metschnikowia pulcherrima is an important yeast species that is attracting increased interest thanks to its biotechnological potential, especially in agri-food applications. Phylogenetically related species of the so-called 'pulcherrima clade' were first described and then reclassified in one single species, which makes the identification an intriguing issue. Starting from the whole-genome sequencing of the protechnological strain Metschnikowia sp. DBT012, this study applied comparative genomics to calculate similarity with the M. pulcherrima clade publicly available genomes with the aim to verify if novel single-copy putative phylogenetic markers could be selected, in comparison with the commonly used primary and secondary barcodes. The genome-based bioinformatic analysis allowed the identification of 85 consensus single-copy orthologs, which were reduced to three after split decomposition analysis. However, wet-lab amplification of these three genes in nonsequenced type strains revealed the presence of multiple copies, which made them unsuitable as phylogenetic markers. Finally, average nucleotide identity (ANI) was calculated between strain DBT012 and available genome sequences of the M. pulcherrima clade, although the genome dataset is still rather limited. Presence of multiple copies of phylogenetic markers as well as ANI values were compatible with the recent reclassification of the clade, allowing the identification of strain DBT012 as M. pulcherrima.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Production of Rhizopus oryzae lipase using optimized Yarrowia lipolytica expression system. 利用优化的解脂耶氏菌表达体系生产米根霉脂肪酶。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad037

Lea Vidal, Zehui Dong, Kim Olofsson, Eva Nordberg Karlsson, Jean-Marc Nicaud

{"title":"Production of Rhizopus oryzae lipase using optimized Yarrowia lipolytica expression system.","authors":"Lea Vidal, Zehui Dong, Kim Olofsson, Eva Nordberg Karlsson, Jean-Marc Nicaud","doi":"10.1093/femsyr/foad037","DOIUrl":"https://doi.org/10.1093/femsyr/foad037","url":null,"abstract":"Yarrowia lipolytica is an alternative yeast for heterologous protein production. Based on auto-cloning vectors, a set of 18 chromogenic cloning vectors was developed, each containing one of the excisable auxotrophic selective markers URA3ex, LYS5ex, and LEU2ex, and one of six different promoters: the constitutive pTEF, the phase dependent hybrid pHp4d, and the erythritol-inducible promoters from pEYK1 and pEYL1 derivatives. These vectors allowed to increase the speed of cloning of the gene of interest. In parallel, an improved new rProt recipient strain JMY8647 was developed by abolishing filamentation and introducing an auxotrophy for lysine (Lys-), providing an additional marker for genetic engineering. Using this cloning strategy, the optimal targeting sequence for Rhizopus oryzae ROL lipase secretion was determined. Among the eight targeting sequences, the SP6 signal sequence resulted in a 23% improvement in the lipase activity compared to that obtained with the wild-type ROL signal sequence. Higher specific lipase activities were obtained using hybrid erythritol-inducible promoters pHU8EYK and pEYL1-5AB, 1.9 and 2.2 times, respectively, when compared with the constitutive pTEF promoter. Two copy strains produce a 3.3 fold increase in lipase activity over the pTEF monocopy strain (266.7 versus 79.7 mU/mg).","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9899748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tuning the expression of the bacterial relBE toxin-antitoxin system in Saccharomyces cerevisiae allows characterizing the subsequent growth inhibition. 调节酿酒酵母中细菌relBE毒素-抗毒素系统的表达，可以表征随后的生长抑制。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad009

Maëlle Duperray, Jean-Marie François, Jean-Pascal Capp

{"title":"Tuning the expression of the bacterial relBE toxin-antitoxin system in Saccharomyces cerevisiae allows characterizing the subsequent growth inhibition.","authors":"Maëlle Duperray, Jean-Marie François, Jean-Pascal Capp","doi":"10.1093/femsyr/foad009","DOIUrl":"https://doi.org/10.1093/femsyr/foad009","url":null,"abstract":"The bacterial toxin-antitoxin systems are each composed of a toxin, which severely inhibits bacterial cells growth, and a specific neutralizing antitoxin. Some toxin-antitoxin systems are functional when expressed in the yeast Saccharomyces cerevisiae. For instance, the expression of the relE toxin gene leads to a strong growth defect in yeast, whereas the expression of the relB antitoxin gene restores growth. Nevertheless, there is no available data regarding the required expression levels of each component of the relBE system leading to these growth phenotypes, neither their effects on cell viability. Here we used a double inducible plasmid-based system to independently modulate the relative amounts of relB and relE, and performed growth and gene expression analyses. These results allow us to correlate growth phenotypes to the expression levels of the toxin and the antitoxin, and to determine the levels necessary to observe either a strong growth inhibition or a normal growth. We also showed that the relE expression produces cell cycle progression defect without affecting cell viability. These results provide a detailed characterization of the functioning of the relBE system in S. cerevisiae, and open applicative perspectives of yeast growth control by bacterial toxin-antitoxin systems.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10822199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of Torulaspora delbrueckii on beer fermentation. 德尔布鲁氏菌对啤酒发酵的影响。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad006

Firuze Kayadelen, Bilal Agirman, Neil P Jolly, Huseyin Erten

{"title":"The influence of Torulaspora delbrueckii on beer fermentation.","authors":"Firuze Kayadelen, Bilal Agirman, Neil P Jolly, Huseyin Erten","doi":"10.1093/femsyr/foad006","DOIUrl":"https://doi.org/10.1093/femsyr/foad006","url":null,"abstract":"In this study, the effect of five different Torulaspora delbrueckii strains in combination with an ale type Saccharomyces cerevisiae on physical, chemical, microbiological, aroma composition, and sensory profiles of beer were examined. The ethyl alcohol content of produced beers ranged from 5.46% (v/v) to 5.93% (v/v), while the highest alcohol amount was obtained using a pure culture of S. cerevisiae. The major volatiles among beer aroma compounds was acetaldehyde, n-propanol, 3-methyl-butanol, 2-methyl-butanol, ethyl acetate, isoamyl acetate, 2,3-butanedione, and 2,3-pentanedione. It was ascertained that the total amount of higher alcohols was higher in the S. cerevisiae control beer compared to all mixed fermentations. Total ester levels were higher in all the mixed culture beers than the control beer. Sensory evaluation showed that all the mixed cultures of S. cerevisiae and T. delbrueckii positively influenced the sensory profile of the beers. Strain Y1031 was the most preferred and was characterized as rich in hop aroma and full bodied. It is therefore a suitable strategy to use T. delbrueckii in mixed fermentations with S. cerevisiae to produce beer with a distinctive flavor. The results demonstrate that, T. delbrueckii strains isolated or commercialized for winemaking can be equally employed as well in brewing.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10875088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1