FEMS yeast research最新文献

Evolutionary Engineering and Molecular Characterization of an Antimycin A-Resistant Saccharomyces cerevisiae Strain: the Key Role of Pleiotropic Drug Resistance (PDR1). 一株抗霉素a耐药酿酒酵母的进化工程和分子特征：多效耐药的关键作用。

IF 2.7 4区生物学

FEMS yeast research Pub Date : 2025-10-17 DOI: 10.1093/femsyr/foaf062

A Topaloğlu, C Holyavkin, Ö Esen, O Morkoç, K Persson, C Geijer, Z P Çakar

{"title":"Evolutionary Engineering and Molecular Characterization of an Antimycin A-Resistant Saccharomyces cerevisiae Strain: the Key Role of Pleiotropic Drug Resistance (PDR1).","authors":"A Topaloğlu, C Holyavkin, Ö Esen, O Morkoç, K Persson, C Geijer, Z P Çakar","doi":"10.1093/femsyr/foaf062","DOIUrl":"https://doi.org/10.1093/femsyr/foaf062","url":null,"abstract":"Antimycin A, an antifungal agent that inhibits mitochondrial respiration, provides a useful model for studying resistance mechanisms. Antifungal resistance is an escalating clinical concern with limited treatment options available. To understand the molecular mechanisms of antimycin A-resistance, a genetically stable, antimycin A-resistant Saccharomyces cerevisiae strain was successfully developed for the first time through an evolutionary engineering strategy, based on long-term systematic application of gradually increasing antimycin A-stress in repetitive batch cultures without prior chemical mutagenesis. Comparative whole genome re-sequencing analysis of the evolved strain ant905-9 revealed two missense mutations in PDR1 and PRP8 genes involved in pleiotropic drug resistance and RNA splicing, respectively. Using CRISPR/Cas9 genome editing tools, the identified mutations were introduced individually and together into the reference strain, and it was confirmed that the Pdr1p.M732R mutation alone confers antimycin A-resistance in S. cerevisiae. Comparative transcriptomic analysis of the reverse engineered Pdr1p.M732R strain showed alterations in PDR (pleiotropic drug response), transmembrane transport, vesicular trafficking and autophagy pathways. Our results highlight the potential key role of PDR1 in antifungal drug resistance. This study provides new insights into mitochondrial drug resistance and the adaptive potential of yeast under respiratory stress.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Southern Hemisphere Yeast Frontier: From nature dwellers to accomplished fermenters. 南半球酵母前沿：从自然居民到成熟的发酵者。

IF 2.7 4区生物学

FEMS yeast research Pub Date : 2025-10-11 DOI: 10.1093/femsyr/foaf063

T Knezevic, P Villarreal, F A Cubillos, C Varela

引用次数: 0

Mechanism of the synergistic action of oxythiamine and ketoconazole against the yeast Malassezia pachydermatis. 氧硫胺素与酮康唑协同防治厚皮马拉色菌的作用机制。

IF 2.7 4区生物学

FEMS yeast research Pub Date : 2025-10-11 DOI: 10.1093/femsyr/foaf059

Magdalena Czerniecka, Adam Więcko, Adam Tylicki

{"title":"Mechanism of the synergistic action of oxythiamine and ketoconazole against the yeast Malassezia pachydermatis.","authors":"Magdalena Czerniecka, Adam Więcko, Adam Tylicki","doi":"10.1093/femsyr/foaf059","DOIUrl":"https://doi.org/10.1093/femsyr/foaf059","url":null,"abstract":"This article explains the biochemical basis of the synergistic effect of oxythiamine and ketoconazole against Malassezia pachydermatis yeast, which was isolated from dogs exhibiting clinical signs of otitis externa. All strains were incubated on MLNA medium supplemented with oxythiamine, ketoconazole or a mixture of both compounds. We found that the ergosterol content was reduced by the compounds tested, both separately (20-50%) and in combination (80%). Oxythiamine alone and in combination with ketoconazole reduced NADPH levels. However, we found no differences in acetyl-CoA levels under the influence of the compounds tested. We suggest that the synergism of oxythiamine and ketoconazole is due to a reduction in the rate of the mevalonate pathway by inhibition of NADPH influx from the pentose phosphate pathway (transketolase inhibition by oxythiamine) and inhibition of C14-α-lanosterol demethylase by ketoconazole. The proposed mechanism may be versatile for other yeasts-like species, making the combination of oxythiamine and ketoconazole a promising treatment option for superficial, opportunistic yeast-like infections.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145274332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yeast - a handy multi-tool in your research: a report from ISSY38. 酵母-在你的研究中一个方便的多工具：来自ISSY38的报告。

IF 2.7 4区生物学

FEMS yeast research Pub Date : 2025-10-08 DOI: 10.1093/femsyr/foaf060

Ewelina Celińska

引用次数: 0

Functional analysis of Saccharomyces cerevisiae FLO genes through optogenetic control. 酿酒酵母FLO基因的光遗传功能分析。

IF 2.7 4区生物学

FEMS yeast research Pub Date : 2025-09-24 DOI: 10.1093/femsyr/foaf057

Denzel G L Ignacia, Nicole X Bennis, Caitlyn Wheeler, Lylyna C L Tu, Jelle Keijzer, Clara Carqueija Cardoso, Jean-Marc G Daran

{"title":"Functional analysis of Saccharomyces cerevisiae FLO genes through optogenetic control.","authors":"Denzel G L Ignacia, Nicole X Bennis, Caitlyn Wheeler, Lylyna C L Tu, Jelle Keijzer, Clara Carqueija Cardoso, Jean-Marc G Daran","doi":"10.1093/femsyr/foaf057","DOIUrl":"https://doi.org/10.1093/femsyr/foaf057","url":null,"abstract":"Flocculation in Saccharomyces cerevisiae is a critical phenotype with ecological and industrial significance. This study aimed to functionally dissect the contributions of individual FLO genes (FLO1, FLO5, FLO9, FLO10) to flocculation by employing an optogenetic circuit (OptoQ-AMP5) for precise, light-inducible control of gene expression. A FLO-null platform yeast strain was engineered allowing the expression of individual FLO genes without native background interference. Each FLO gene was reintroduced into the FLO-null background under the control of OptoQ-AMP5. Upon light induction, strains expressing FLO1, FLO5, or FLO10 demonstrated strong flocculation, with FLO1 and FLO5 forming large and structurally distinct aggregates. FLO9 induced a weaker phenotype. Sugar inhibition assays revealed distinct sensitivities among flocculins, notably FLO9's novel sensitivity to fructose and maltotriose. Additionally, FLO-induced changes in cell surface hydrophobicity were quantified, revealing that FLO10 and FLO1 conferred the greatest hydrophobicity, correlating with their aggregation strength. This work establishes a robust platform for investigating flocculation mechanisms in yeast with temporal precision. It highlights the phenotypic diversity encoded within the FLO gene family and their differential responses to environmental cues. The optogenetic system provides a valuable tool for both fundamental studies and the rational engineering of yeast strains for industrial fermentation processes requiring controlled flocculation.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145130367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel method for telomere length detection in fission yeast. 裂变酵母端粒长度检测的新方法。

IF 2.4 4区生物学

FEMS yeast research Pub Date : 2025-01-30 DOI: 10.1093/femsyr/foae040

Hadeel A B Elnaim Mohamed, Hizlan Hincal Agus, Bedia Palabiyik

{"title":"A novel method for telomere length detection in fission yeast.","authors":"Hadeel A B Elnaim Mohamed, Hizlan Hincal Agus, Bedia Palabiyik","doi":"10.1093/femsyr/foae040","DOIUrl":"10.1093/femsyr/foae040","url":null,"abstract":"Fission yeast is the ideal model organism for studying telomere maintenance in higher eukaryotes. Telomere length has been directly correlated with life expectancy and the onset of aging-related diseases in mammals. In this study, we developed a novel simple, and reproducible method to measure the telomere length, by investigating the effect of caffeine and cisplatin on the telomere length in fission yeast. Hydroxyurea-synchronized fission yeast cells were exposed to 62 µM cisplatin and 8.67 mM caffeine treatments for 2 h, then their telomere lengths were evaluated with two different methods. First, the quantitative polymerase chain reaction (qPCR) assay was used as a confirmative method, where telomere length was determined relative to a single-copy gene in the genome. Second, the newly developed method standard polymerase chain reaction (PCR)/ImageJ assay assessed the telomere length based on the amplified PCR band intensity using a set of telomere primers, reflecting telomeric sequence availability in the genome. Both methods show a significant decrease and a notable telomere lengthening in response to cisplatin and caffeine treatments, respectively. The finding supports the accuracy and productivity of the standard PCR/ImageJ assay as it can serve as a quick screening tool to study the effect of suspected chemotherapeutic and antiaging drugs on telomere length in fission yeast.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11781191/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and phenotypic diversity of wine-associated Hanseniaspora species. 葡萄酒相关菌的遗传和表型多样性。

IF 2.4 4区生物学

FEMS yeast research Pub Date : 2025-01-30 DOI: 10.1093/femsyr/foaf031

Cristobal A Onetto, Chris M Ward, Cristian Varela, Laura Hale, Simon A Schmidt, Anthony R Borneman

{"title":"Genetic and phenotypic diversity of wine-associated Hanseniaspora species.","authors":"Cristobal A Onetto, Chris M Ward, Cristian Varela, Laura Hale, Simon A Schmidt, Anthony R Borneman","doi":"10.1093/femsyr/foaf031","DOIUrl":"10.1093/femsyr/foaf031","url":null,"abstract":"The genus Hanseniaspora includes apiculate yeasts commonly found in fruit- and fermentation-associated environments. Their genetic diversity and evolutionary adaptations remain largely unexplored despite their ecological and oenological significance. This study investigated the phylogenetic relationships, genome structure, selection patterns, and phenotypic diversity of Hanseniaspora species isolated primarily from Australian wine environments, focusing on Hanseniaspora uvarum, the most abundant non-Saccharomyces yeast in wine fermentation. A total of 151 isolates were sequenced, including long-read genomes for representatives of the main phylogenetic clades. Comparative genomics revealed ancestral chromosomal rearrangements between the slow-evolving lineage (SEL) and fast-evolving lineage (FEL) that could have contributed to their evolutionary split, as well as significant loss of genes associated with mRNA splicing, chromatid segregation and signal recognition particle protein targeting in the FEL. Pangenome analysis within H. uvarum identified extensive copy number variation, particularly in genes related to xenobiotic tolerance and nutrient transport. Investigation into the selective landscape following the FEL/SEL divergence identified diversifying selection in 229 genes in the FEL, with significant enrichment in genes within the lysine biosynthetic pathway. Furthermore, phenotypic screening of 116 isolates revealed substantial intraspecific diversity, with specific species exhibiting enhanced ethanol, osmotic, copper, SO₂, and cold tolerance.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12199727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic versatility of the Wickerhamiella/Starmerella yeast clade: from sugar utilization to lipid metabolism. Wickerhamiella/Starmerella酵母分支的代谢多样性：从糖利用到脂质代谢。

IF 2.7 4区生物学

FEMS yeast research Pub Date : 2025-01-30 DOI: 10.1093/femsyr/foaf048

Meriam Vandeputte, Emma Diependaele, Inge Noëlle Adriënne Van Bogaert

{"title":"Metabolic versatility of the Wickerhamiella/Starmerella yeast clade: from sugar utilization to lipid metabolism.","authors":"Meriam Vandeputte, Emma Diependaele, Inge Noëlle Adriënne Van Bogaert","doi":"10.1093/femsyr/foaf048","DOIUrl":"10.1093/femsyr/foaf048","url":null,"abstract":"The Wickerhamiella/Starmerella (W/S) yeast clade has recently gained attention as a \"treasure trove\" of metabolic diversity, characterized by unusual pathways shaped through complex evolutionary events. One of their most distinctive traits is their specialized sugar metabolism, which allows them to thrive in sugar-rich environments. While their role in sugar utilization is well established, emerging evidence suggests that some W/S species can also metabolize hydrophobic compounds, indicating a broader capacity for lipid-related processes. For instance, several W/S species produce sophorolipids (SLs)-a class of glycolipids with commercial applications as sustainable biosurfactants. This ability has sparked growing interest in their potential to synthesize a diverse range of lipids, including glycolipids and dicarboxylic acids. This mini-review explores the evolution and distinctive metabolic features of the W/S clade, focusing not only on its well-characterized sugar metabolism but also on its lesser-known lipid metabolism. Particular attention is given to SL production and the expansion and diversity of cytochrome P450 family 52 enzymes within this underexplored group, emphasizing their biotechnological potential in lipid biosynthesis and their promising applications in sustainable bioprocesses.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12461148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reviving Élie Metschnikoff's Monospora: the obligately parasitic yeast Australozyma monospora sp. nov. 复兴Élie Metschnikoff的单孢子菌：专性寄生酵母菌单孢子Australozyma。

IF 2.7 4区生物学

FEMS yeast research Pub Date : 2025-01-30 DOI: 10.1093/femsyr/foaf041

Marc-André Lachance, Carla E Cáceres, Molly J Fredericks, Meghan A Duffy, Tara E Stewart Merrill

{"title":"Reviving Élie Metschnikoff's Monospora: the obligately parasitic yeast Australozyma monospora sp. nov.","authors":"Marc-André Lachance, Carla E Cáceres, Molly J Fredericks, Meghan A Duffy, Tara E Stewart Merrill","doi":"10.1093/femsyr/foaf041","DOIUrl":"10.1093/femsyr/foaf041","url":null,"abstract":"A vast literature explores a model system that consists of a prey crustacean, the water flea Daphnia spp., and an obligately pathogenic yeast that has been referred to as Metschnikowia bicuspidata and thought to represent the material used by Metschnikoff in his study of innate immunity. Typification of species bearing that name and indeed the whole genus has been problematic as regards yeasts that only grow or form aciculate ascospores in hospite. The neotype of M. bicuspidata, unlike the Daphnia parasite, is easily cultured on a variety of laboratory media, although it too can cause serious infections in a variety of mostly aquatic animals. It has become evident that the Daphnia parasite studied by Metschnikoff or current workers is not closely related to M. bicuspidata as currently understood. Analysis of whole genome DNA extracted from the yeast repeatedly found in infected Daphnia specimens shows that it belongs to the recently circumscribed genus Australozyma. The yeast is described here as Australozyma monospora sp. nov. The species, although haplontic and heterothallic, forms single-spored asci without mating. It also appears that all species in the genus are restricted to asexual reproduction, which may explain their rare status. The holotype is MICH 346683. The name is registered in Mycobank under the number MB 859667.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":"25 ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12359140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144872197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermotolerant yeasts promoting climate-resilient bioproduction. 促进气候适应型生物生产的耐热酵母。

IF 2.7 4区生物学

FEMS yeast research Pub Date : 2025-01-30 DOI: 10.1093/femsyr/foaf047

Roghayeh Shirvani, Maryam Babaei, Motahare Baladi, Matthias G Steiger, Mohammad Barshan-Tashnizi

{"title":"Thermotolerant yeasts promoting climate-resilient bioproduction.","authors":"Roghayeh Shirvani, Maryam Babaei, Motahare Baladi, Matthias G Steiger, Mohammad Barshan-Tashnizi","doi":"10.1093/femsyr/foaf047","DOIUrl":"10.1093/femsyr/foaf047","url":null,"abstract":"The growing challenges posed by global warming and the demand for sustainable food and feed resources underscore the need for robust microbial platforms in bioprocessing. Thermotolerant yeasts have emerged as promising candidates due to their ability to thrive at elevated temperatures and other industrially relevant stresses. This review examines the industrial potential of thermotolerant yeasts in the context of climate change, emphasizing how their resilience can lead to more energy-efficient and cost-effective bioprocesses. Particular attention is paid to the thermodynamic implications of yeast metabolism under heat stress, especially in bioethanol production and methanol metabolism in methylotrophic yeasts, where metabolic heat generation plays a critical role. The cellular and molecular mechanisms underlying thermotolerance are also reviewed, including heat shock sensing mechanisms, the protection of biomolecules, and membrane and cell wall integrity. Advances in genetic and metabolic engineering aimed at enhancing these traits are also highlighted. By integrating current insights into the molecular and cellular mechanisms of thermotolerance, along with recent technological advancements, this review outlines the advantages of high-temperature operations and positions thermotolerant yeasts as vital components of future sustainable bioproduction systems.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12449156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0