FEMS yeast research最新文献_第10页

Improving the Z3EV promoter system to create the strongest yeast promoter. 改进 Z3EV 启动子系统，创建最强的酵母启动子。

IF 2.4 4区生物学

FEMS yeast research Pub Date : 2024-01-09 DOI: 10.1093/femsyr/foae032

Rina Higuchi, Yuri Fujita, Shotaro Namba, Hisao Moriya

{"title":"Improving the Z3EV promoter system to create the strongest yeast promoter.","authors":"Rina Higuchi, Yuri Fujita, Shotaro Namba, Hisao Moriya","doi":"10.1093/femsyr/foae032","DOIUrl":"10.1093/femsyr/foae032","url":null,"abstract":"Promoters for artificial control of gene expression are central tools in genetic engineering. In the budding yeast Saccharomyces cerevisiae, a variety of constitutive and controllable promoters with different strengths have been constructed using endogenous gene promoters, synthetic transcription factors and their binding sequences, and artificial sequences. However, there have been no attempts to construct the highest strength promoter in yeast cells. In this study, by incrementally increasing the binding sequences of the synthetic transcription factor Z3EV, we were able to construct a promoter (P36) with ~1.4 times the strength of the TDH3 promoter. This is stronger than any previously reported promoter. Although the P36 promoter exhibits some leakage in the absence of induction, the expression induction by estradiol is maintained. When combined with a multicopy plasmid, it can express up to ~50% of total protein as a heterologous protein. This promoter system can be used to gain knowledge about the cell physiology resulting from the ultimate overexpression of excess proteins and is expected to be a useful tool for heterologous protein expression in yeast.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11523633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific growth rates and growth stoichiometries of Saccharomycotina yeasts on ethanol as sole carbon and energy substrate. 乙醇作为唯一碳和能量底物的酵母菌的特定生长速率和生长化学计量学。

IF 2.4 4区生物学

FEMS yeast research Pub Date : 2024-01-09 DOI: 10.1093/femsyr/foae037

Marieke Warmerdam, Marcel A Vieira-Lara, Robert Mans, Jean Marc Daran, Jack T Pronk

{"title":"Specific growth rates and growth stoichiometries of Saccharomycotina yeasts on ethanol as sole carbon and energy substrate.","authors":"Marieke Warmerdam, Marcel A Vieira-Lara, Robert Mans, Jean Marc Daran, Jack T Pronk","doi":"10.1093/femsyr/foae037","DOIUrl":"10.1093/femsyr/foae037","url":null,"abstract":"Emerging low-emission production technologies make ethanol an interesting substrate for yeast biotechnology, but information on growth rates and biomass yields of yeasts on ethanol is scarce. Strains of 52 Saccharomycotina yeasts were screened for growth on ethanol. The 21 fastest strains, among which representatives of the Phaffomycetales order were overrepresented, showed specific growth rates in ethanol-grown shake-flask cultures between 0.12 and 0.46 h-1. Seven strains were studied in aerobic, ethanol-limited chemostats (dilution rate 0.10 h-1). Saccharomyces cerevisiae and Kluyveromyces lactis, whose genomes do not encode Complex-I-type NADH dehydrogenases, showed biomass yields of 0.59 and 0.56 gbiomass gethanol-1, respectively. Different biomass yields were observed among species whose genomes do harbour Complex-I-encoding genes: Phaffomyces thermotolerans (0.58 g g-1), Pichia ethanolica (0.59 g g-1), Saturnispora dispora (0.66 g g-1), Ogataea parapolymorpha (0.67 g g-1), and Cyberlindnera jadinii (0.73 g g-1). Cyberlindnera jadinii biomass showed the highest protein content (59 ± 2%) of these yeasts. Its biomass yield corresponded to 88% of the theoretical maximum that is reached when growth is limited by assimilation rather than by energy availability. This study suggests that energy coupling of mitochondrial respiration and its regulation will become key factors for selecting and improving yeast strains for ethanol-based processes.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11657237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142828164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of an economical xylose-utilizing Saccharomyces cerevisiae and its ethanol fermentation. 构建经济型木糖利用酿酒酵母菌及其乙醇发酵。

IF 2.4 4区生物学

FEMS yeast research Pub Date : 2024-01-09 DOI: 10.1093/femsyr/foae001

Fan Li, Wenxin Bai, Yuan Zhang, Zijian Zhang, Deguo Zhang, Naidong Shen, Jingwei Yuan, Guomiao Zhao, Xiaoyan Wang

{"title":"Construction of an economical xylose-utilizing Saccharomyces cerevisiae and its ethanol fermentation.","authors":"Fan Li, Wenxin Bai, Yuan Zhang, Zijian Zhang, Deguo Zhang, Naidong Shen, Jingwei Yuan, Guomiao Zhao, Xiaoyan Wang","doi":"10.1093/femsyr/foae001","DOIUrl":"10.1093/femsyr/foae001","url":null,"abstract":"Traditional industrial Saccharomyces cerevisiae could not metabolize xylose due to the lack of a specific enzyme system for the reaction from xylose to xylulose. This study aims to metabolically remould industrial S. cerevisiae for the purpose of utilizing both glucose and xylose with high efficiency. Heterologous gene xylA from Piromyces and homologous genes related to xylose utilization were selected to construct expression cassettes and integrated into genome. The engineered strain was domesticated with industrial material under optimizing conditions subsequently to further improve xylose utilization rates. The resulting S. cerevisiae strain ABX0928-0630 exhibits a rapid growth rate and possesses near 100% xylose utilization efficiency to produce ethanol with industrial material. Pilot-scale fermentation indicated the predominant feature of ABX0928-0630 for industrial application, with ethanol yield of 0.48 g/g sugars after 48 hours and volumetric xylose consumption rate of 0.87 g/l/h during the first 24 hours. Transcriptome analysis during the modification and domestication process revealed a significant increase in the expression level of pathways associated with sugar metabolism and sugar sensing. Meanwhile, genes related to glycerol lipid metabolism exhibited a pattern of initial increase followed by a subsequent decrease, providing a valuable reference for the construction of efficient xylose-fermenting strains.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10855017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139546141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using Euf1 transcription factor as a titrator of erythritol-inducible promoters in Yarrowia lipolytica; insight into the structure, splicing, and regulation mechanism. 利用 Euf1 转录因子作为脂肪溶解亚罗藻中赤藓糖醇诱导启动子的滴定剂；深入了解结构、剪接和调控机制。

IF 2.4 4区生物学

FEMS yeast research Pub Date : 2024-01-09 DOI: 10.1093/femsyr/foae027

Ewelina Celińska, Paulina Korpys-Woźniak, Maria Gorczyca, Jean-Marc Nicaud

{"title":"Using Euf1 transcription factor as a titrator of erythritol-inducible promoters in Yarrowia lipolytica; insight into the structure, splicing, and regulation mechanism.","authors":"Ewelina Celińska, Paulina Korpys-Woźniak, Maria Gorczyca, Jean-Marc Nicaud","doi":"10.1093/femsyr/foae027","DOIUrl":"10.1093/femsyr/foae027","url":null,"abstract":"Controllable regulatory elements, like inducible, titratable promoters, are highly desired in synthetic biology toolboxes. A set of previously developed erythritol-inducible promoters along with an engineered Yarrowia lipolytica host strain were shown to be a very potent expression platform. In this study, we push the previously encountered limits of the synthetic promoters' titratability (by the number of upstream motifs) by using a compatible transcription factor, Euf1, as the promoter titrator. Overexpression of spliced EUF1 turned out to be very efficient in promoting expression from the compatible promoter, however, the erythritol-inducible character of the promoter was then lost. Analysis of the EUF1's splicing pattern suggests that the intron removal is promoted in the presence of erythritol, but is not dependent on it. The 3D structures of spliced versus unspliced Euf1 were modeled, and ligand-binding strength was calculated and compared. Furthermore, the EUF1-dependent expression profile under different chemical stimulants was investigated. Depletion of carbon source was identified as the significant factor upregulating the expression from the Euf1-dependent promoter (2-10-fold). Considering these findings and transcriptomics data, a new mechanism of the Euf1-regulated promoter action is proposed, involving a 'catabolite repression' transcription factor-Adr1, both acting on the same ERY-inducible promoter.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11394100/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142016873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fermenting the future - on the benefits of a bioart collaboration. 发酵未来--生物艺术合作的益处。

IF 2.4 4区生物学

FEMS yeast research Pub Date : 2024-01-09 DOI: 10.1093/femsyr/foae004

Diethard Mattanovich, Martin Altvater, Özge Ata, Simone Bachleitner

引用次数: 0

A novel fluorescence-activated cell sorting (FACS)-based screening identified ATG14, the gene required for pexophagy in the methylotrophic yeast. 一种基于荧光激活细胞分选（FACS）的新型筛选方法发现了ATG14，它是甲基营养酵母中进行pexophagy所需的基因。

IF 2.4 4区生物学

FEMS yeast research Pub Date : 2024-01-09 DOI: 10.1093/femsyr/foae022

Kosuke Shiraishi, Yumi Arima, Motoharu Nakamura, Takumi Nakatsuji, Masahide Oku, Yasuyoshi Sakai

{"title":"A novel fluorescence-activated cell sorting (FACS)-based screening identified ATG14, the gene required for pexophagy in the methylotrophic yeast.","authors":"Kosuke Shiraishi, Yumi Arima, Motoharu Nakamura, Takumi Nakatsuji, Masahide Oku, Yasuyoshi Sakai","doi":"10.1093/femsyr/foae022","DOIUrl":"10.1093/femsyr/foae022","url":null,"abstract":"Pexophagy is a type of autophagy that selectively degrades peroxisomes and can be classified as either macropexophagy or micropexophagy. During macropexophagy, individual peroxisomes are sequestered by pexophagosomes and transported to the vacuole for degradation, while in micropexophagy, peroxisomes are directly engulfed by the septated vacuole. To date, some autophagy-related genes (ATGs) required for pexophagy have been identified through plate-based assays performed primarily under micropexophagy-induced conditions. Here, we developed a novel high-throughput screening system using fluorescence-activated cell sorting (FACS) to identify genes required for macropexophagy. Using this system, we discovered KpATG14, a gene that could not be identified previously in the methylotrophic yeast Komagataella phaffii due to technical limitations. Microscopic and immunoblot analyses found that KpAtg14 was required for both macropexophagy and micropexophagy. We also revealed that KpAtg14 was necessary for recruitment of the downstream factor KpAtg5 at the preautophagosomal structure (PAS), and consequently, for bulk autophagy. We anticipate our assay to be used to identify novel genes that are exclusively required for macropexophagy, leading to better understanding of the physiological significance of the existing two types of autophagic degradation pathways for peroxisomes.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11305268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141723350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Data integration strategies for whole-cell modeling. 全细胞建模的数据整合策略。

IF 2.4 4区生物学

FEMS yeast research Pub Date : 2024-01-09 DOI: 10.1093/femsyr/foae011

Katja Tummler, Edda Klipp

引用次数: 0

Heterologous pulcherrimin production in Saccharomyces cerevisiae confers inhibitory activity on Botrytis conidiation. 在酿酒酵母中异源生产 pulcherrimin 可对肉毒菌分生孢子产生抑制作用。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2024-01-09 DOI: 10.1093/femsyr/foad053

Florian M Freimoser, Marina Mahler, Mark McCullough, Alexander O Brachmann, Lukas Nägeli, Maja Hilber-Bodmer, Jörn Piel, Stefan A Hoffmann, Yizhi Cai

{"title":"Heterologous pulcherrimin production in Saccharomyces cerevisiae confers inhibitory activity on Botrytis conidiation.","authors":"Florian M Freimoser, Marina Mahler, Mark McCullough, Alexander O Brachmann, Lukas Nägeli, Maja Hilber-Bodmer, Jörn Piel, Stefan A Hoffmann, Yizhi Cai","doi":"10.1093/femsyr/foad053","DOIUrl":"10.1093/femsyr/foad053","url":null,"abstract":"Pulcherrimin is an iron (III) chelate of pulcherriminic acid that plays a role in antagonistic microbial interactions, iron metabolism, and stress responses. Some bacteria and yeasts produce pulcherriminic acid, but so far, pulcherrimin could not be produced in Saccharomyces cerevisiae. Here, multiple integrations of the Metschnikowia pulcherrima PUL1 and PUL2 genes in the S. cerevisiae genome resulted in red colonies, which indicated pulcherrimin formation. The coloration correlated positively and significantly with the number of PUL1 and PUL2 genes. The presence of pulcherriminic acid was confirmed by mass spectrometry. In vitro competition assays with the plant pathogenic fungus Botrytis caroliana revealed inhibitory activity on conidiation by an engineered, strong pulcherrimin-producing S. cerevisiae strain. We demonstrate that the PUL1 and PUL2 genes from M. pulcherrima, in multiple copies, are sufficient to transfer pulcherrimin production to S. cerevisiae and represent the starting point for engineering and optimizing this biosynthetic pathway in the future.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10786192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138884786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Yca1 metacaspase: diverse functions determine how yeast live and let die. 更正：Yca1元天冬酶：多种功能决定酵母如何生存和死亡

IF 2.4 4区生物学

FEMS yeast research Pub Date : 2024-01-09 DOI: 10.1093/femsyr/foae019

引用次数: 0

Riboflavin overproduction on lignocellulose hydrolysate by the engineered yeast Candida famata. 工程酵母法氏念珠菌在木质纤维素水解物上过量产生核黄素。

IF 2.4 4区生物学

FEMS yeast research Pub Date : 2024-01-09 DOI: 10.1093/femsyr/foae020

Ljubov S Dzanaeva, Dominik Wojdyła, Dariya V Fedorovych, Justyna Ruchala, Kostyantyn V Dmytruk, Andriy A Sibirny

{"title":"Riboflavin overproduction on lignocellulose hydrolysate by the engineered yeast Candida famata.","authors":"Ljubov S Dzanaeva, Dominik Wojdyła, Dariya V Fedorovych, Justyna Ruchala, Kostyantyn V Dmytruk, Andriy A Sibirny","doi":"10.1093/femsyr/foae020","DOIUrl":"10.1093/femsyr/foae020","url":null,"abstract":"Lignocellulose (dry plant biomass) is an abundant cheap inedible residue of agriculture and wood industry with great potential as a feedstock for biotechnological processes. Lignocellulosic substrates can serve as valuable resources in fermentation processes, allowing the production of a wide array of chemicals, fuels, and food additives. The main obstacle for cost-effective conversion of lignocellulosic hydrolysates to target products is poor metabolism of the major pentoses, xylose and L-arabinose, which are the second and third most abundant sugars of lignocellulose after glucose. We study the oversynthesis of riboflavin in the flavinogenic yeast Candida famata and found that all major lignocellulosic sugars, including xylose and L-arabinose, support robust growth and riboflavin synthesis in the available strains of C. famata. To further increase riboflavin production from xylose and lignocellulose hydrolysate, genes XYL1 and XYL2 coding for xylose reductase and xylitol dehydrogenase were overexpressed. The resulting strains exhibited increased riboflavin production in both shake flasks and bioreactors using diluted hydrolysate, reaching 1.5 g L-1.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11283204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0