FEMS yeast research最新文献_第10页

How do engineered Yarrowia lipolytica strains secrete free fatty acids: hints from comparative transcriptomics. 工程多脂耶氏菌菌株如何分泌游离脂肪酸:来自比较转录组学的提示。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad027

José Manuel Salvador López, Lea Vidal, Michelle Patricia Adiutama, Filip Van Nieuwerburgh, Dieter Deforce, Jean-Marc Nicaud, Inge Noëlle Adrienne Van Bogaert

{"title":"How do engineered Yarrowia lipolytica strains secrete free fatty acids: hints from comparative transcriptomics.","authors":"José Manuel Salvador López, Lea Vidal, Michelle Patricia Adiutama, Filip Van Nieuwerburgh, Dieter Deforce, Jean-Marc Nicaud, Inge Noëlle Adrienne Van Bogaert","doi":"10.1093/femsyr/foad027","DOIUrl":"https://doi.org/10.1093/femsyr/foad027","url":null,"abstract":"Yarrowia lipolytica has been considered one of the most promising platforms for the microbial production of fatty acids and derived products. The deletion of the faa1 gene coding for an acyl-CoA synthetase leads to the accumulation and secretion of free fatty acids (FFAs) into the extracellular space. The secretion of products is beneficial for the development of microbial cell factories to avoid intracellular inhibitory effects and reduce downstream processing costs. However, the mechanism behind the secretion of fatty acids is not well known. As a starting point, we compared the transcriptome of this mutant showing FFA secretion to a wildtype-like strain not showing this phenotype. The 12 most upregulated genes were evaluated for involvement in FFA secretion by the creation of deletion and overexpression mutants, among them MCH2, YMOH, three cell wall proteins CWP3, CWP4, and CWP11, M12B, and three proteins with unknown functions YUP1, YUP2, and YUP3. None of these proteins take a clear or isolated role in FFA export. As the transcriptomic data revealed an overrepresentation of cell wall-related proteins, some of them were further examined on a theoretical and experimental way. Surprisingly, overexpression of Ygpi led to the production of FFAs in the wildtype-like genetic background. Finally, some of the evaluated genes showed involvement in resistance to FFA toxicity.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":"23 ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10033779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tuning the expression of the bacterial relBE toxin-antitoxin system in Saccharomyces cerevisiae allows characterizing the subsequent growth inhibition. 调节酿酒酵母中细菌relBE毒素-抗毒素系统的表达，可以表征随后的生长抑制。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad009

Maëlle Duperray, Jean-Marie François, Jean-Pascal Capp

{"title":"Tuning the expression of the bacterial relBE toxin-antitoxin system in Saccharomyces cerevisiae allows characterizing the subsequent growth inhibition.","authors":"Maëlle Duperray, Jean-Marie François, Jean-Pascal Capp","doi":"10.1093/femsyr/foad009","DOIUrl":"https://doi.org/10.1093/femsyr/foad009","url":null,"abstract":"The bacterial toxin-antitoxin systems are each composed of a toxin, which severely inhibits bacterial cells growth, and a specific neutralizing antitoxin. Some toxin-antitoxin systems are functional when expressed in the yeast Saccharomyces cerevisiae. For instance, the expression of the relE toxin gene leads to a strong growth defect in yeast, whereas the expression of the relB antitoxin gene restores growth. Nevertheless, there is no available data regarding the required expression levels of each component of the relBE system leading to these growth phenotypes, neither their effects on cell viability. Here we used a double inducible plasmid-based system to independently modulate the relative amounts of relB and relE, and performed growth and gene expression analyses. These results allow us to correlate growth phenotypes to the expression levels of the toxin and the antitoxin, and to determine the levels necessary to observe either a strong growth inhibition or a normal growth. We also showed that the relE expression produces cell cycle progression defect without affecting cell viability. These results provide a detailed characterization of the functioning of the relBE system in S. cerevisiae, and open applicative perspectives of yeast growth control by bacterial toxin-antitoxin systems.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":"23 ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10822199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Finding a correct species assignment for a Metschnikowia strain: insights from the genome sequencing of strain DBT012. 为Metschnikowia菌株寻找正确的物种分配:来自菌株DBT012基因组测序的见解。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad024

Eleonora Troiano, Ilaria Larini, Renato L Binati, Veronica Gatto, Sandra Torriani, Pietro Buzzini, Benedetta Turchetti, Elisa Salvetti, Giovanna E Felis

{"title":"Finding a correct species assignment for a Metschnikowia strain: insights from the genome sequencing of strain DBT012.","authors":"Eleonora Troiano, Ilaria Larini, Renato L Binati, Veronica Gatto, Sandra Torriani, Pietro Buzzini, Benedetta Turchetti, Elisa Salvetti, Giovanna E Felis","doi":"10.1093/femsyr/foad024","DOIUrl":"https://doi.org/10.1093/femsyr/foad024","url":null,"abstract":"Metschnikowia pulcherrima is an important yeast species that is attracting increased interest thanks to its biotechnological potential, especially in agri-food applications. Phylogenetically related species of the so-called 'pulcherrima clade' were first described and then reclassified in one single species, which makes the identification an intriguing issue. Starting from the whole-genome sequencing of the protechnological strain Metschnikowia sp. DBT012, this study applied comparative genomics to calculate similarity with the M. pulcherrima clade publicly available genomes with the aim to verify if novel single-copy putative phylogenetic markers could be selected, in comparison with the commonly used primary and secondary barcodes. The genome-based bioinformatic analysis allowed the identification of 85 consensus single-copy orthologs, which were reduced to three after split decomposition analysis. However, wet-lab amplification of these three genes in nonsequenced type strains revealed the presence of multiple copies, which made them unsuitable as phylogenetic markers. Finally, average nucleotide identity (ANI) was calculated between strain DBT012 and available genome sequences of the M. pulcherrima clade, although the genome dataset is still rather limited. Presence of multiple copies of phylogenetic markers as well as ANI values were compatible with the recent reclassification of the clade, allowing the identification of strain DBT012 as M. pulcherrima.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":"23 ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Importance and mechanisms of S-adenosylmethionine and folate accumulation in sake yeast. s -腺苷蛋氨酸和叶酸在清酒酵母中积累的重要性及其机制。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad004

Muneyoshi Kanai, Masaki Mizunuma, Tsutomu Fujii, Haruyuki Iefuji

{"title":"Importance and mechanisms of S-adenosylmethionine and folate accumulation in sake yeast.","authors":"Muneyoshi Kanai, Masaki Mizunuma, Tsutomu Fujii, Haruyuki Iefuji","doi":"10.1093/femsyr/foad004","DOIUrl":"https://doi.org/10.1093/femsyr/foad004","url":null,"abstract":"Sake yeasts have a range of brewing characteristics that are particularly beneficial for sake making including high ethanol fermentability, high proliferative capacity at low temperatures, lactic acid tolerance, and high ester productivity. On the other hand, sake yeasts also accumulate a diverse range of functional components. For example, significantly greater accumulation of S-adenosylmethionine (SAM), a compound that plays important regulatory roles in a range of biological processes as a major donor of methyl groups, occurs in sake yeasts compared to other microorganisms. Significantly greater accumulation of folate, a bioactive water-soluble vitamin (vitamin B9), also occurs in sake yeasts compared to laboratory yeasts, and the methyl group on SAM is supplied by folate. Accordingly, fully characterizing 'sake yeast identity' requires detailed understanding of the mechanisms underlying both the nutritional characteristics (functional components) and the brewing characteristics in sake yeasts. Therefore, this mini-review focuses on the accumulation of SAM and folate in sake yeast including descriptions of the genes known to contribute to SAM and folate accumulation and the underlying mechanisms.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":"23 ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9072103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biology and physiology of Hanseniaspora vineae: metabolic diversity and increase flavour complexity for food fermentation. 汉森菌的生物学和生理学:食物发酵的代谢多样性和增加风味的复杂性。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad010

Francisco Carrau, Eduardo Dellacassa, Eduardo Boido, Karina Medina, Maria Jose Valera, Laura Fariña, Gabriel Perez, Valentina Martin, Fernando Alvarez-Valin, Lucia Balestrazzi

{"title":"Biology and physiology of Hanseniaspora vineae: metabolic diversity and increase flavour complexity for food fermentation.","authors":"Francisco Carrau, Eduardo Dellacassa, Eduardo Boido, Karina Medina, Maria Jose Valera, Laura Fariña, Gabriel Perez, Valentina Martin, Fernando Alvarez-Valin, Lucia Balestrazzi","doi":"10.1093/femsyr/foad010","DOIUrl":"https://doi.org/10.1093/femsyr/foad010","url":null,"abstract":"Apiculate yeasts belonging to the genus Hanseniaspora are predominant on grapes and other fruits. While some species, such as Hanseniaspora uvarum, are well known for their abundant presence in fruits, they are generally characterized by their detrimental effect on fermentation quality because the excessive production of acetic acid. However, the species Hanseniaspora vineae is adapted to fermentation and currently is considered as an enhancer of positive flavour and sensory complexity in foods. Since 2002, we have been isolating strains from this species and conducting winemaking processes with them. In parallel, we also characterized this species from genes to metabolites. In 2013, we sequenced the genomes of two H. vineae strains, being these the first apiculate yeast genomes determined. In the last 10 years, it has become possible to understand its biology, discovering very peculiar features compared to the conventional Saccharomyces yeasts, such as a natural and unique G2 cell cycle arrest or the elucidation of the mandelate pathway for benzenoids synthesis. All these characteristics contribute to phenotypes with proved interest from the biotechnological point of view for winemaking and the production of other foods.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":"23 ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9117502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Identification of the main proteins secreted by Kluyveromyces marxianus and their possible roles in antagonistic activity against fungi. 马氏克鲁维菌主要分泌蛋白的鉴定及其可能的拮抗作用。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad007

Ana Maria Dos Santos, Fernanda Matias Albuini, Géssica Cabral Barros, Olinto Liparini Pereira, Wendel Batista da Silveira, Luciano Gomes Fietto

{"title":"Identification of the main proteins secreted by Kluyveromyces marxianus and their possible roles in antagonistic activity against fungi.","authors":"Ana Maria Dos Santos, Fernanda Matias Albuini, Géssica Cabral Barros, Olinto Liparini Pereira, Wendel Batista da Silveira, Luciano Gomes Fietto","doi":"10.1093/femsyr/foad007","DOIUrl":"https://doi.org/10.1093/femsyr/foad007","url":null,"abstract":"Lytic enzymes secreted by Kluyveromyces marxianus can lyse Saccharomyces cerevisiae cells. Their ability to hydrolyze yeast cell walls can be used in biotechnological applications, such as the production of glucans and protoplasts, as well as a biological control agent against plant pathogenic fungi. Herein, 27 proteins secreted by K. marxianus were identified by mass spectrometry analyses. Importantly, 14 out of the 27 proteins were classified as hydrolases. Indeed, the enzyme extract secreted by K. marxianus caused damage to S. cerevisiae cells and reduced yeast cell viability. Moreover, K marxianus inhibited spore germination and mycelial growth of the phytopathogenic fungus Botrytis cinerea in simultaneous cocultivation assays. We suggest that this inhibition may be partially related to the yeast's ability to secrete lytic enzymes. Consistent with the in vitro antagonistic tests, K. marxianus was able to protect strawberry fruits inoculated with B. cinerea. Therefore, these findings suggest that K. marxianus possesses potential as a biocontrol agent against strawberry gray mold during the postharvest stage and may also have potential against other phytopathogenic fungi by means of its lytic enzymatic arsenal.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":"23 ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10810127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metformin extends the chronological lifespan of fission yeast by altering energy metabolism and stress resistance capacity. 二甲双胍通过改变能量代谢和抗逆性来延长裂变酵母的寿命。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad018

Ceren Şeylan, Çağatay Tarhan

{"title":"Metformin extends the chronological lifespan of fission yeast by altering energy metabolism and stress resistance capacity.","authors":"Ceren Şeylan, Çağatay Tarhan","doi":"10.1093/femsyr/foad018","DOIUrl":"https://doi.org/10.1093/femsyr/foad018","url":null,"abstract":"The antiaging properties of metformin used for the treatment of type-2 diabetes mellitus have been studied extensively, but there is more to discover regarding underlying mechanisms. Here, we show that metformin significantly prolongs the chronological lifespan (CLS) of Schizosaccharomyces pombe through mechanisms similar to those observed in mammalian cells and other model organisms. While the presence of metformin in the medium caused an increase in carbohydrate consumption and ATP production, it reduced reactive oxygen species production and alleviate oxidative damage parameters such as lipid peroxidation and carbonylated proteins. We also tested whether the effect of metformin changed with the time it was added to the medium and observed that the lifespan-prolonging effect of metformin was related to the glucose concentration in the medium and did not prolong lifespan when added after glucose was completely depleted in the medium. On the other hand, cells inoculated in glucose-free medium containing metformin also showed extended lifespan suggesting that mechanisms other than that solely depend on glucose availability may be involved in extending the lifespan. These results suggest that metformin prolongs lifespan especially affecting energy metabolism and stress resistance capacity and that fission yeast can be effectively used when investigating the antiaging mechanisms of metformin.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":"23 ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10103822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9678037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Malassezia sympodialis Mala s 1 allergen is a potential KELCH protein that cross reacts with human skin. 马拉色菌马拉 s 1 过敏原是一种潜在的 KELCH 蛋白，会与人体皮肤发生交叉反应。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad028

Dora E Corzo Leon, Annika Scheynius, Donna M MacCallum, Carol A Munro

{"title":"Malassezia sympodialis Mala s 1 allergen is a potential KELCH protein that cross reacts with human skin.","authors":"Dora E Corzo Leon, Annika Scheynius, Donna M MacCallum, Carol A Munro","doi":"10.1093/femsyr/foad028","DOIUrl":"10.1093/femsyr/foad028","url":null,"abstract":"Malassezia are the dominant commensal yeast species of the human skin microbiota and are associated with inflammatory skin diseases, such as atopic eczema (AE). The Mala s 1 allergen of Malassezia sympodialis is a β-propeller protein, inducing both IgE and T-cell reactivity in AE patients. We demonstrate by immuno-electron microscopy that Mala s 1 is mainly located in the M. sympodialis yeast cell wall. An anti-Mala s 1 antibody did not inhibit M. sympodialis growth suggesting Mala s 1 may not be an antifungal target. In silico analysis of the predicted Mala s 1 protein sequence identified a motif indicative of a KELCH protein, a subgroup of β-propeller proteins. To test the hypothesis that antibodies against Mala s 1 cross-react with human skin (KELCH) proteins we examined the binding of the anti-Mala s 1 antibody to human skin explants and visualized binding in the epidermal skin layer. Putative human targets recognized by the anti-Mala s 1 antibody were identified by immunoblotting and proteomics. We propose that Mala s 1 is a KELCH-like β-propeller protein with similarity to human skin proteins. Mala s 1 recognition may trigger cross-reactive responses that contribute to skin diseases associated with M. sympodialis.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":"23 ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10281499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10116538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using the AKAR3-EV biosensor to assess Sch9p- and PKA-signalling in budding yeast. 利用 AKAR3-EV 生物传感器评估芽殖酵母中的 Sch9p 和 PKA 信号。

IF 2.4 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad029

Dennis Botman, Sineka Kanagasabapathi, Philipp Savakis, Bas Teusink

{"title":"Using the AKAR3-EV biosensor to assess Sch9p- and PKA-signalling in budding yeast.","authors":"Dennis Botman, Sineka Kanagasabapathi, Philipp Savakis, Bas Teusink","doi":"10.1093/femsyr/foad029","DOIUrl":"10.1093/femsyr/foad029","url":null,"abstract":"Budding yeast uses the TORC1-Sch9p and cAMP-PKA signalling pathways to regulate adaptations to changing nutrient environments. Dynamic and single-cell measurements of the activity of these cascades will improve our understanding of the cellular adaptation of yeast. Here, we employed the AKAR3-EV biosensor developed for mammalian cells to measure the cellular phosphorylation status determined by Sch9p and PKA activity in budding yeast. Using various mutant strains and inhibitors, we show that AKAR3-EV measures the Sch9p- and PKA-dependent phosphorylation status in intact yeast cells. At the single-cell level, we found that the phosphorylation responses are homogenous for glucose, sucrose, and fructose, but heterogeneous for mannose. Cells that start to grow after a transition to mannose correspond to higher normalized Förster resonance energy transfer (FRET) levels, in line with the involvement of Sch9p and PKA pathways to stimulate growth-related processes. The Sch9p and PKA pathways have a relatively high affinity for glucose (K0.5 of 0.24 mM) under glucose-derepressed conditions. Lastly, steady-state FRET levels of AKAR3-EV seem to be independent of growth rates, suggesting that Sch9p- and PKA-dependent phosphorylation activities are transient responses to nutrient transitions. We believe that the AKAR3-EV sensor is an excellent addition to the biosensor arsenal for illuminating cellular adaptation in single yeast cells.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":"23 ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10237333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9775002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable production of single-cell oil and protein from wastepaper hydrolysate: identification and optimization of a Rhodotorula mucilaginosa strain as a promising yeast. 从废纸水解物中可持续生产单细胞油和蛋白质：一株有前景的粘液红酵母菌株的鉴定和优化。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-01-04 DOI: 10.1093/femsyr/foad044

Amador Campos-Valdez, Manuel R Kirchmayr, Iliana Barrera-Martínez, Leticia Casas-Godoy

{"title":"Sustainable production of single-cell oil and protein from wastepaper hydrolysate: identification and optimization of a Rhodotorula mucilaginosa strain as a promising yeast.","authors":"Amador Campos-Valdez, Manuel R Kirchmayr, Iliana Barrera-Martínez, Leticia Casas-Godoy","doi":"10.1093/femsyr/foad044","DOIUrl":"10.1093/femsyr/foad044","url":null,"abstract":"This study investigated the potential of wastepaper hydrolysate as a sustainable and low-cost carbon source for single-cell oil and protein production, attending to the growing need for alternative feedstocks and waste management strategies. Wastepaper, characterized by its high carbohydrate content, was subjected to enzymatic and chemo-enzymatic treatments for carbohydrate release. The chemo-enzymatic treatment performed better, yielding 65.3 g l-1 of fermentable sugars. A total of 62 yeast strains were screened for single-cell oil accumulation, identifying Rhodotorula mucilaginosa M1K4 as the most advantageous oleaginous yeast. M1K4 lipid production was optimized in liquid culture, and its fatty acid profile was analyzed, showing a high content of industrially valuable fatty acids, particularly palmitic (28%) and oleic (51%). Batch-culture of M1K4 in a 3-l reactor demonstrated the strain's ability to utilize wastepaper hydrolysate as a carbon source, with dry cell weight, total lipid and protein production of 17.7 g l-1, 4.5 g l-1, and 2.1 g l-1, respectively. Wastepaper as a substrate provides a sustainable solution for waste management and bioproduction. This research highlights the potential of R. mucilaginosa for lipid and protein production from wastepaper hydrolysate.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41104581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0